LOCUS       sequence-419431-        3010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector to store Level 0 broad-host range L. plantarum 
            replication origin parts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Li X, Schonberg PY, Wucherpfennig T, Hinze C, Sulaj F, Henle T, 
            Mascher T
  TITLE     Development of a Golden Gate Assembly-Based Genetic Toolbox for 
            Lactiplantibacillus plantarum and Its Application for Engineering 
            Monoterpenoid Biosynthesis.
  JOURNAL   ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 
            10.1021/acssynbio.4c00075. Epub 2024 Sep 10.
  PUBMED    39254046
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00075"; journalName: "ACS Synth Biol"; date: 
            "2024-09-20- 20"; volume: "13"; issue: "9"; pages: "2764-2779"
COMMENT     Sequence Label: pLP3
FEATURES             Location/Qualifiers
     source          1..3010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(167..261)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(282..941)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     856..875
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1103..1121
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1125..1168)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1171..1192
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             1363..1374
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1381..2094
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2106..2177
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2193..2220
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(2235..2252)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2406..2994)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2486..2505)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttctacggg gtctgacgga agcgtctcat gagacccacg ttaagggatt ttggtcatga
       61 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
      121 tctaaagtat atatgagtaa acttggtctg acagctcgag gcttggattc tcaccaataa
      181 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
      241 ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac
      301 tcgtcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
      361 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
      421 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
      481 gtgaaactca cccagggatt ggctgatacg aaaaacatat tctcaataaa ccctttaggg
      541 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
      601 cggaaatcgt cgtggtattc actccagagt gatgaaaacg tttcagtttg ctcatggaaa
      661 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
      721 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
      781 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
      841 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
      901 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
      961 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     1021 ttggaacctc ttacgtgccc gatcaactcg agtgccacct gacgtctaag aaaccattat
     1081 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
     1141 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agcgatgagt
     1201 ggtctcgtga gacggaaagt gaaacgtgat ttcatgcgtc attttgaaca ttttgtaaat
     1261 cttatttaat aatgtgtgcg gcaattcaca tttaatttat gaatgttttc ttaacatcgc
     1321 ggcaactcaa gaaacggcag gttcggatct tagctactag agaaagagga gaaatactag
     1381 atgcgtaaag gcgaagagct gttcactggt gtcgtcccta ttctggtgga actggatggt
     1441 gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt
     1501 aaactgacgc tgaagttcat ctgtactact ggtaaactgc cggttccttg gccgactctg
     1561 gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt atccggacca tatgaagcag
     1621 catgacttct tcaagtccgc catgccggaa ggctatgtgc aggaacgcac gatttccttt
     1681 aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta
     1741 aaccgcattg agctgaaagg cattgacttt aaagaggacg gcaatatcct gggccataag
     1801 ctggaataca attttaacag ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc
     1861 attaaagcga attttaaaat tcgccacaac gtggaggatg gcagcgtgca gctggctgat
     1921 cactaccagc aaaacactcc aatcggtgat ggtcctgttc tgctgccaga caatcactat
     1981 ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga aacgcgatca tatggttctg
     2041 ctggagttcg taaccgcagc gggcatcacg catggtatgg atgaactgta caaatgacca
     2101 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     2161 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     2221 tttatacagg attcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2281 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2341 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2401 gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2461 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2521 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2581 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     2641 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     2701 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     2761 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     2821 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     2881 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     2941 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3001 cctttgatct
//
