LOCUS       sequence-419436-        2950 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Level 0 Part: E. coli shuttle part (pMB1_ampR_B0017) for 
            the construction of Level 1 plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Li X, Schonberg PY, Wucherpfennig T, Hinze C, Sulaj F, Henle T, 
            Mascher T
  TITLE     Development of a Golden Gate Assembly-Based Genetic Toolbox for 
            Lactiplantibacillus plantarum and Its Application for Engineering 
            Monoterpenoid Biosynthesis.
  JOURNAL   ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 
            10.1021/acssynbio.4c00075. Epub 2024 Sep 10.
  PUBMED    39254046
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00075"; journalName: "ACS Synth Biol"; date: 
            "2024-09-20- 20"; volume: "13"; issue: "9"; pages: "2764-2779"
COMMENT     Sequence Label: pLPE1
FEATURES             Location/Qualifiers
     source          1..2950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             155..166
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      880..951
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      967..994
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        1047..1151
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1152..1220,1221..2012)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1370..1389)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2183..2771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2672..2691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      2861..2932
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tcaccggaaa gtgaaacgtg atttcatgcg tcattttgaa cattttgtaa atcttattta
       61 ataatgtgtg cggcaattca catttaattt atgaatgttt tcttaacatc gcggcaactc
      121 aagaaacggc aggttcggat cttagctact agagaaagag gagaaatact agatggtttc
      181 taaaggtgaa gcagtgatca aagaatttat gcgcttcaaa gttcacatgg aaggttctat
      241 gaacggccac gaatttgaaa ttgaaggtga aggcgaaggt cgtccatacg aaggtactca
      301 aaccgcaaaa ctgaaagtta ccaaaggtgg tccactgcca ttttcttggg atattctgtc
      361 tccacaattt atgtacggtt ctcgtgcatt tatcaaacac ccagcagata ttccagacta
      421 ctacaaacaa tcttttccgg aaggtttcaa atgggaacgt gttatgaatt ttgaagatgg
      481 tggtgcagtt acggttaccc aagatacctc tctggaagat ggtactctga tctacaaagt
      541 taaactgcgt ggtactaact ttccaccaga tggtccagtt atgcagaaaa aaaccatggg
      601 ttgggaagca tctaccgaac gtctgtaccc agaagatggc gttctgaaag gtgatatcaa
      661 aatggcactg cgtctgaaag atggcggtcg ttacctggca gatttcaaaa ccacctacaa
      721 agcgaaaaaa ccagttcaaa tgccaggtgc atacaacgtt gatcgtaaac tggatattac
      781 cagccacaac gaagattaca ccgttgttga acaatacgaa cgttctgaag gccgtcactc
      841 taccggtggt atggatgaac tgtacaaata accaggcatc aaataaaacg aaaggctcag
      901 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      961 cacactggct caccttcggg tgggcctttc tgcgtttata caggtctcag gtcgaccgaa
     1021 gacgttcctt tttcggggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     1081 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1141 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     1201 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1261 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1321 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1381 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1441 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1501 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1561 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1621 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1681 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1741 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1801 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1861 agcgtggttc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1921 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1981 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     2041 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     2101 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     2161 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     2221 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2281 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     2341 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2401 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2461 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2521 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2581 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2641 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2701 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2761 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2821 ttgctcacat gttctttcct gaagacgttc ctccaggcat caaataaaac gaaaggctca
     2881 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagta
     2941 acatgagacc
//
