LOCUS       sequence-41947-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, 
            Eichtinger M, Jiang T, Foley JE, Winfrey RJ, Townsend JA, 
            Unger-Wallace E, Sander JD, Muller-Lerch F, Fu F, Pearlberg J, 
            Goebel C, Dassie JP, Pruett-Miller SM, Porteus MH, Sgroi DC, Iafrate
            AJ, Dobbs D, McCray PB, Cathomen T, Voytas DF, Joung JK
  TITLE     Rapid ""open-source"" engineering of customized zinc-finger 
            nucleases for highly efficient gene modification.
  JOURNAL   Mol Cell. 2008 Jul 25. 31(2):294-301.
  PUBMED    18657511
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Jul 25. 31(2):294-301."
COMMENT     Sequence Label: dio2_L (OZ569)
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..68
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(557..576)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attgtgcaaa ggccctgaga aaaatccaag ctttggcggc cgctgactac aaagacgatg
       61 atgacaaatc tagacccggg gagcgcccct tccagtgtcg catttgcatg cggaactttt
      121 cgaccaaaca gattttgggt cgtcataccc gtactcatac cggtgaaaaa ccgtttcagt
      181 gtcggatctg tatgcgaaat ttctcccagt ctaccacctt gaaacgtcat ctacgtacgc
      241 acaccggcga gaagccattc caatgccgaa tatgcatgcg caacttcagt cagaaaccac
      301 atttgtctcg tcacctaaaa acccacctga ggggatcctc tgcacagtag taactagaga
      361 ctagaaaaag gccgacaagt cccgctccgc tgaagatcct ggcgtaatag cgaagaggcc
      421 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
      481 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
      541 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
      601 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
      661 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
      721 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actct
//
