LOCUS       sequence-419481-        8687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of pLEX302_circMTO1_OE expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8687)
  AUTHORS   Liu L, Neve M, Perlaza-Jimenez L, Xi X, Purcell J, Hawdon A, Conn 
            SJ, Zenker J, Tamayo P, Goodall GJ, Rosenbluh J
  TITLE     Systematic loss-of-function screens identify pathway-specific 
            functional circular RNAs.
  JOURNAL   Nat Cell Biol. 2024 Aug;26(8):1359-1372. doi: 
            10.1038/s41556-024-01467-y. Epub 2024 Aug 2.
  PUBMED    39095657
REFERENCE   2  (bases 1 to 8687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-024-01467-y"; journalName: "Nat Cell Biol"; date: 
            "2024-08"; volume: "26"; issue: "8"; pages: "1359-1372"
COMMENT     Sequence Label: pLEX302_circMTO1_OE
FEATURES             Location/Qualifiers
     source          1..8687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(258..269)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             447..680
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    758..879
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(795..814)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     849..868
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      919..1054
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1074..1093)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1075..1093)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1103..1120)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1103..1119)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1112..1134)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1261..1716
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1348..1367)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1558..1579
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1742..1846
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1847..1915,1916..2707)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2065..2084)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2878..3466
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3367..3386
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3620..3637
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3754..3775
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3790..3795,3796..3813,3814..3820)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3828..3844
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3833..3855
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3852..3868
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3852..3868
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3887..3907
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3889..3907
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3935..4161
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4162..4342
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4389..4514
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5007..5240
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5425..5469
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5644..6144
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6063..6081
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6166..6765
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6166..6185)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6662..6682
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6807..6924
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        7041..7344
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7345..7548
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7499..7519
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    7571..7595
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    8138..8362
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(8365..8389)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8391..8432
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    join(8474..8687,1..375)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8527..8547)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
       61 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
      121 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
      181 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
      241 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
      301 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
      361 gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag
      421 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
      481 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
      541 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
      601 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
      661 agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt
      721 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
      781 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
      841 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
      901 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
      961 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     1021 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     1081 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     1141 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     1201 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     1261 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     1321 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     1381 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     1441 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     1501 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     1561 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     1621 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     1681 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     1741 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     1801 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     1861 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     1921 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     1981 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     2041 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     2101 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     2161 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     2221 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     2281 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     2341 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     2401 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     2461 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     2521 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     2581 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     2641 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     2701 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     2761 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     2821 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2881 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2941 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3001 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     3061 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3121 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3181 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3241 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3301 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3361 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3421 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3481 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3541 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3601 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     3661 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     3721 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     3781 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     3841 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     3901 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     3961 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     4021 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     4081 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
     4141 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
     4201 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     4261 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     4321 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     4381 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     4441 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     4501 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     4561 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     4621 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     4681 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     4741 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     4801 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     4861 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     4921 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     4981 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     5041 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     5101 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     5161 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     5221 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     5281 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     5341 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     5401 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     5461 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     5521 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     5581 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     5641 cccttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
     5701 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
     5761 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
     5821 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
     5881 gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga
     5941 ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg
     6001 gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg
     6061 cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac
     6121 cgaatcaccg acctctctcc ccagggggta ccaccggagc ttaccatgac cgagtacaag
     6181 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     6241 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6301 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6361 gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6421 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6481 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6541 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6601 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6661 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6721 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgagaatt ctagatcttg
     6781 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     6841 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     6901 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
     6961 cgccggctcg agggggcccg gggttattaa tagtaatcaa ttacggggtc attagttcat
     7021 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     7081 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     7141 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     7201 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     7261 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     7321 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     7381 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     7441 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     7501 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
     7561 gtcgggatca acaagtttgt acaaaaaagt tggcaaagtg ctgagattac aggcgtgagc
     7621 caccaccccc ggcccacttt ttgtaaaggt acgtactaat gacttttttt tatacttcag
     7681 gtcagatgtc atgtaatcct tcctttggtg gcatcggaaa gggacattta atgagggaag
     7741 tagatgcctt ggatggcctg tgttctcgca tctgtgacca gtctggtgta cattataaag
     7801 tattaaaccg gcgtaaggga ccagctgtgt ggggtctgag agctcagatt gataggaaac
     7861 tctataaaca gaacatgcag gtaagaatag ggcatgagca caggaaagat tatagtgatt
     7921 gtttaattac catgtttcaa ctggcatttt cttttgacag aaagaaatct tgaatacacc
     7981 actgcttact gttcaggagg gagctgtaga agatcttatt cttacagaac cagagcctga
     8041 acacactggg aaatgccgtg tcagtggggt tgttttgggt aagaagcaag gaaaagaatt
     8101 aggctcggca cggtagctca cacctgtaat cccagcactg tgccttctag ttgccagcca
     8161 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     8221 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     8281 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     8341 ggggatgcgg tgggctctat ggacccccaa ctttcttgta caaagtggtt ggtaagccta
     8401 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc
     8461 gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     8521 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     8581 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     8641 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgca
//
