LOCUS       sequence-41949-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, 
            Eichtinger M, Jiang T, Foley JE, Winfrey RJ, Townsend JA, 
            Unger-Wallace E, Sander JD, Muller-Lerch F, Fu F, Pearlberg J, 
            Goebel C, Dassie JP, Pruett-Miller SM, Porteus MH, Sgroi DC, Iafrate
            AJ, Dobbs D, McCray PB, Cathomen T, Voytas DF, Joung JK
  TITLE     Rapid ""open-source"" engineering of customized zinc-finger 
            nucleases for highly efficient gene modification.
  JOURNAL   Mol Cell. 2008 Jul 25. 31(2):294-301.
  PUBMED    18657511
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Jul 25. 31(2):294-301."
COMMENT     Sequence Label: dio2_R (OZ570)
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..69
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     rep_origin      471..851
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(558..577)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     768..789
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attgtgcaaa ggccctgaag aaaaatccaa gctttggcgg ccgctgacta caaagacgat
       61 gatgacaaat ctagacccgg ggagcgcccc ttccagtgtc gcatttgcat gcggaacttt
      121 tcgatgaagc accaccttgt cgtacatacc cgtactcata ccggtgaaaa accgtttcag
      181 tgtcggatct gtatgcgaaa tttctccgtg gcgcaccact tgaagcgcca tctacgtacg
      241 cacaccggcg agaagccatt ccaatgccga atatgcatgc gcaacttcag taacccgccc
      301 cacctgaacg gccacctaaa aacccacctg aggggatcct ctgcacagta gtaactagag
      361 actagaaaaa ggccgacaag tcccgctccg ctgaagatcc tggcgtaata gcgaagaggc
      421 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
      481 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
      541 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
      601 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
      661 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
      721 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
      781 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
      841 gccgatttcg gcctattggt taaaaaatga gctga
//
