LOCUS       sequence-419490-        2130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Level 0 Part: Ribosome binding site (RB0034) for the 
            construction of Level 1 plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Stukenberg D, Hensel T, Hoff J, Daniel B, Inckemann R, Tedeschi JN, 
            Nousch F, Fritz G
  TITLE     The Marburg Collection: A Golden Gate DNA Assembly Framework for 
            Synthetic Biology Applications in Vibrio natriegens.
  JOURNAL   ACS Synth Biol. 2021 Aug 20;10(8):1904-1919. doi: 
            10.1021/acssynbio.1c00126. Epub 2021 Jul 13.
  PUBMED    34255476
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00126"; journalName: "ACS Synth Biol"; date: 
            "2021-08-20- 20"; volume: "10"; issue: "8"; pages: "1904-1919"
COMMENT     Sequence Label: pLPS1
FEATURES             Location/Qualifiers
     source          1..2130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             46..57
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      104..161
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(185..202)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(356..944)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(436..455)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1127..1221)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1242..1901)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1816..1835
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2063..2081
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2085..2128)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gaattcgcgg ccgcttctag agcgatgagt ggtctcgtac tagagaaaga ggagaaataa
       61 tcaatgtgag accactcatc gcgactagta gcggccgctg cagtccggca aaaaagggca
      121 aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg
      181 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      241 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      301 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      361 acaggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      421 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      481 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      541 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      601 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
      661 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
      721 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
      781 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
      841 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      901 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
      961 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     1021 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     1081 tctaaagtat atatgagtaa acttggtctg acagctcgag gcttggattc tcaccaataa
     1141 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
     1201 ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac
     1261 tcgtcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
     1321 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
     1381 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
     1441 gtgaaactca cccagggatt ggctgatacg aaaaacatat tctcaataaa ccctttaggg
     1501 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
     1561 cggaaatcgt cgtggtattc actccagagt gatgaaaacg tttcagtttg ctcatggaaa
     1621 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     1681 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     1741 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     1801 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     1861 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     1921 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     1981 ttggaacctc ttacgtgccc gatcaactcg agtgccacct gacgtctaag aaaccattat
     2041 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
     2101 atccttagct ttcgctaagg atgatttctg
//
