LOCUS       sequence-419491-        3127 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector to store Level 0 parts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Stukenberg D, Hensel T, Hoff J, Daniel B, Inckemann R, Tedeschi JN, 
            Nousch F, Fritz G
  TITLE     The Marburg Collection: A Golden Gate DNA Assembly Framework for 
            Synthetic Biology Applications in Vibrio natriegens.
  JOURNAL   ACS Synth Biol. 2021 Aug 20;10(8):1904-1919. doi: 
            10.1021/acssynbio.1c00126. Epub 2021 Jul 13.
  PUBMED    34255476
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00126"; journalName: "ACS Synth Biol"; date: 
            "2021-08-20- 20"; volume: "10"; issue: "8"; pages: "1904-1919"
COMMENT     Sequence Label: pLP1
FEATURES             Location/Qualifiers
     source          1..3127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             163..174
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             181..894
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      906..977
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      993..1020
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      1071..1128
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1152..1169)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1323..1911)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1403..1422)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2094..2188)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2209..2868)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2783..2802
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3030..3048
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3052..3095)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3098..3119
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgtga gacggaaagt gaaacgtgat ttcatgcgtc attttgaaca ttttgtaaat
       61 cttatttaat aatgtgtgcg gcaattcaca tttaatttat gaatgttttc ttaacatcgc
      121 ggcaactcaa gaaacggcag gttcggatct tagctactag agaaagagga gaaatactag
      181 atgcgtaaag gcgaagagct gttcactggt gtcgtcccta ttctggtgga actggatggt
      241 gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt
      301 aaactgacgc tgaagttcat ctgtactact ggtaaactgc cggttccttg gccgactctg
      361 gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt atccggacca tatgaagcag
      421 catgacttct tcaagtccgc catgccggaa ggctatgtgc aggaacgcac gatttccttt
      481 aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta
      541 aaccgcattg agctgaaagg cattgacttt aaagaggacg gcaatatcct gggccataag
      601 ctggaataca attttaacag ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc
      661 attaaagcga attttaaaat tcgccacaac gtggaggatg gcagcgtgca gctggctgat
      721 cactaccagc aaaacactcc aatcggtgat ggtcctgttc tgctgccaga caatcactat
      781 ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga aacgcgatca tatggttctg
      841 ctggagttcg taaccgcagc gggcatcacg catggtatgg atgaactgta caaatgacca
      901 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      961 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1021 tttatacgtc tcatgagacc actcatcgcg actagtagcg gccgctgcag tccggcaaaa
     1081 aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt
     1141 atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     1201 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     1261 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1321 tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1381 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1441 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1501 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1561 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1621 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1681 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1741 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1801 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1861 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1921 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1981 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     2041 aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct tggattctca
     2101 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     2161 gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc
     2221 ctgccactcg tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     2281 acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     2341 atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
     2401 aaaactggtg aaactcaccc agggattggc tgatacgaaa aacatattct caataaaccc
     2461 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
     2521 aaactgccgg aaatcgtcgt ggtattcact ccagagtgat gaaaacgttt cagtttgctc
     2581 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
     2641 tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
     2701 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
     2761 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
     2821 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
     2881 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
     2941 gtgaaagttg gaacctctta cgtgcccgat caactcgagt gccacctgac gtctaagaaa
     3001 ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat
     3061 aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg cttctagagc
     3121 gatgagt
//
