LOCUS       sequence-419493-        2120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Level 0 Part: 5' Connector (5C2SN) for the construction of 
            Level 1 plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Stukenberg D, Hensel T, Hoff J, Daniel B, Inckemann R, Tedeschi JN, 
            Nousch F, Fritz G
  TITLE     The Marburg Collection: A Golden Gate DNA Assembly Framework for 
            Synthetic Biology Applications in Vibrio natriegens.
  JOURNAL   ACS Synth Biol. 2021 Aug 20;10(8):1904-1919. doi: 
            10.1021/acssynbio.1c00126. Epub 2021 Jul 13.
  PUBMED    34255476
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00126"; journalName: "ACS Synth Biol"; date: 
            "2021-08-20- 20"; volume: "10"; issue: "8"; pages: "1904-1919"
COMMENT     Sequence Label: pLPC52
FEATURES             Location/Qualifiers
     source          1..2120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      94..151
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(175..192)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(346..934)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(426..445)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1117..1211)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1232..1891)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1806..1825
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2053..2071
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2075..2118)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gaattcgcgg ccgcttctag agcgatgagt ggtctcgaac acgtctcgta ctggagtgag
       61 accactcatc gcgactagta gcggccgctg cagtccggca aaaaagggca aggtgtcacc
      121 accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct
      181 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      241 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      301 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg
      361 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      421 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      481 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      541 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      601 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      661 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
      721 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
      781 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
      841 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
      901 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
      961 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     1021 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1081 atatgagtaa acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg
     1141 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
     1201 caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcgtcgcagt
     1261 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
     1321 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
     1381 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     1441 cccagggatt ggctgatacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     1501 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     1561 cgtggtattc actccagagt gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     1621 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg
     1681 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     1741 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     1801 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     1861 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     1921 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
     1981 ttacgtgccc gatcaactcg agtgccacct gacgtctaag aaaccattat tatcatgaca
     2041 ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct
     2101 ttcgctaagg atgatttctg
//
