LOCUS       sequence-419494-        9032 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing gRNA targeting human SMARCA4 and 
            SMARCA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9032)
  AUTHORS   Aoki K, Hyuga M, Tarumoto Y, Nishibuchi G, Ueda A, Ochi Y, Sugino S,
            Mikami T, Kobushi H, Kato I, Akahane K, Inukai T, Takaori-Kondo A, 
            Takita J, Ogawa S, Yusa K
  TITLE     Canonical BAF complex regulates the oncogenic program in human 
            T-cell acute lymphoblastic leukemia.
  JOURNAL   Blood. 2023 Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857.
  PUBMED    37922452
REFERENCE   2  (bases 1 to 9032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2023
            Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857."
COMMENT     Sequence Label: 
            pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(SMARCA2(46))-PGKpuroBFP-W
FEATURES             Location/Qualifiers
     source          1..9032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     624..643
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        624..642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        958..1156
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1111..1131
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1174..1354
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1401..1526
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2025..2258
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2443..2487
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2785..2902
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3359..3607
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3359..3379
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3530..3549
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        3736..4235
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3771..3794)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4168..4187
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4275..4871
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4275..4294)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4771..4791
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4890..4943
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4944..5645
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(5705..5725)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6123..6134)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6586..6603)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6592..6816
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6842..6862)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6842..6860)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6881..6897)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6881..6897)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6894..6916)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6905..6921
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6929..6935,6936..6953,6954..6959))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6974..6995
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7112..7129)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7283..7871)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7363..7382)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8042..8833,8834..8902))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8665..8684
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8903..9007)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg actagtacat
      661 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      721 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      781 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      841 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      901 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      961 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     1021 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     1081 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     1141 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
     1201 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     1261 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     1321 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     1381 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     1441 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     1501 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     1561 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     1621 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     1681 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1741 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1801 ccaaggaagc tttagacaag atagaggaac agcaaaacaa aagtaagacc accgcacagc
     1861 aagcggccgg ccgcgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     1921 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     1981 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     2041 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     2101 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     2161 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     2221 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     2281 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     2341 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     2401 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     2461 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     2521 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     2581 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     2641 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     2701 gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag
     2761 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2821 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2881 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt
     2941 agtaccgggc cctacgcgtc tgcagtattt agcatgcccc acccatctgc aaggcattct
     3001 ggatagtgtc aaaacagccg gaaatcaagt ccgtttatct caaactttag cattttggga
     3061 ataaatgata tttgctatgc tggttaaatt agattttagt taaatttcct gctgaagctc
     3121 tagtacgata agcaacttga cctaagtgta aagttgagac ttccttcagg tttatatagc
     3181 ttgtgcgccg cttgggtacc tcgatcagaa cgctgtccaa ctgtttaaga gctatgctgg
     3241 aaacagcata gcaagtttaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
     3301 gtcggtgctt tttttagccg aactgtttca cactcacgcg tccaaggtcg ggcaggaaga
     3361 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
     3421 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
     3481 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
     3541 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
     3601 aaacaccgct cccttgtccc ccagctagtt taagagctat gctggaaaca gcatagcaag
     3661 tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt
     3721 ggatccaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
     3781 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
     3841 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     3901 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     3961 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     4021 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     4081 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     4141 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     4201 cgctgttctc ctcttcctca tctccgggcc tttcgacctg catccatcta gatctcgagc
     4261 agctgaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     4321 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     4381 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     4441 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     4501 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     4561 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     4621 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     4681 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     4741 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     4801 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     4861 agcccggtgc cggcggcggg tccggaggag agggcagagg aagtctccta acatgcggtg
     4921 acgtggagga gaatcctggc ccaatgagcg agctgattaa ggagaacatg cacatgaagc
     4981 tgtacatgga gggcaccgtg gacaaccatc acttcaagtg cacatccgag ggcgaaggca
     5041 agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct
     5101 tcgccttcga catcctggct actagcttcc tctacggcag caagaccttc atcaaccaca
     5161 cccagggcat ccccgacttc ttcaagcagt ccttccctga gggcttcaca tgggagagag
     5221 tcaccacata cgaggacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg
     5281 gctgcctcat ctacaacgtc aagatcagag gggtgaactt cacatccaac ggccctgtga
     5341 tgcagaagaa aacactcggc tgggaggcct tcaccgagac gctgtacccc gctgacggcg
     5401 gcctggaagg cagaaacgac atggccctga agctcgtggg cgggagccat ctgatcgcaa
     5461 acatcaagac cacatataga tccaagaaac ccgctaagaa cctcaagatg cctggcgtct
     5521 actatgtgga ctacagactg gaaagaatca aggaggccaa caacgagacc tacgtcgagc
     5581 agcacgaggt ggcagtggcc agatactgcg acctccctag caaactgggg cacaagctta
     5641 attgagcggc cgctcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     5701 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     5761 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     5821 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     5881 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     5941 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6001 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     6061 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6121 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6181 ttccgcgtct acgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     6241 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     6301 aaaagggggg actggaaggg ctaattcact cccaaagaag tcaagatctg ctttttgcct
     6361 gtactgggtc tctctggtta gaccagagtc tctctggtta gaccagatct gagcctggga
     6421 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6481 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6541 ttagtcagtg tggaaaatct ctagcagttt aaacccgctg atcagcctcg actgtgcctt
     6601 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     6661 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     6721 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagtca
     6781 atagcaggca tgctggggat gcggtgggct ctatgggcgg ccgttaatta agcttttgtt
     6841 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     6901 gaaattgtta tccgctcaca attccacaca acatacgagc cgggagcata aagtgtaaag
     6961 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7021 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7081 gcggtttgcg tattgggcgc tgttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7141 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7201 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7261 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7321 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7381 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7441 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7501 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7561 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7621 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7681 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     7741 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7801 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7861 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7921 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7981 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8041 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8101 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8161 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8221 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8281 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8341 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8401 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8461 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8521 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8581 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8641 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8701 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8761 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8821 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8881 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     8941 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9001 ttccgcgcac atttccccga aaagtgccac ct
//
