LOCUS       sequence-419495-        2150 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Level 0 Part: Terminator (TB1006) for the construction of 
            Level 1 plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Stukenberg D, Hensel T, Hoff J, Daniel B, Inckemann R, Tedeschi JN, 
            Nousch F, Fritz G
  TITLE     The Marburg Collection: A Golden Gate DNA Assembly Framework for 
            Synthetic Biology Applications in Vibrio natriegens.
  JOURNAL   ACS Synth Biol. 2021 Aug 20;10(8):1904-1919. doi: 
            10.1021/acssynbio.1c00126. Epub 2021 Jul 13.
  PUBMED    34255476
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00126"; journalName: "ACS Synth Biol"; date: 
            "2021-08-20- 20"; volume: "10"; issue: "8"; pages: "1904-1919"
COMMENT     Sequence Label: pLPT1
FEATURES             Location/Qualifiers
     source          1..2150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      124..181
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(205..222)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(376..964)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(456..475)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1147..1241)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1262..1921)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1836..1855
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2083..2101
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2105..2148)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gaattcgcgg ccgcttctag agcgatgagt ggtctcggct taaaaaaaaa aaccccgccc
       61 ctgacagggc ggggtttttt ttcgcttgag accactcatc gcgactagta gcggccgctg
      121 cagtccggca aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
      181 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      241 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      301 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      361 cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg
      421 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      481 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      541 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      601 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      661 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      721 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      781 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      841 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      901 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      961 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     1021 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     1081 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag
     1141 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     1201 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
     1261 attacgcccc gccctgccac tcgtcgcagt actgttgtaa ttcattaagc attctgccga
     1321 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     1381 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     1441 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgatacg aaaaacatat
     1501 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     1561 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagt gatgaaaacg
     1621 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     1681 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
     1741 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     1801 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     1861 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     1921 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     1981 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaactcg agtgccacct
     2041 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     2101 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg
//
