LOCUS       sequence-419496-        2823 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Level 0 Part: Coding sequences (sfGFP) for the construction
            of Level 1 plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Stukenberg D, Hensel T, Hoff J, Daniel B, Inckemann R, Tedeschi JN, 
            Nousch F, Fritz G
  TITLE     The Marburg Collection: A Golden Gate DNA Assembly Framework for 
            Synthetic Biology Applications in Vibrio natriegens.
  JOURNAL   ACS Synth Biol. 2021 Aug 20;10(8):1904-1919. doi: 
            10.1021/acssynbio.1c00126. Epub 2021 Jul 13.
  PUBMED    34255476
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00126"; journalName: "ACS Synth Biol"; date: 
            "2021-08-20- 20"; volume: "10"; issue: "8"; pages: "1904-1919"
COMMENT     Sequence Label: pLP-sfgfp
FEATURES             Location/Qualifiers
     source          1..2823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             9..722
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      767..824
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(848..865)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1019..1607)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1099..1118)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1790..1884)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1905..2564)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2479..2498
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2726..2744
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2748..2791)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2794..2815
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgaat gcgtaaaggt gaagaactgt ttaccggtgt tgttccaatt ctggttgaac
       61 tggatggtga tgttaacggt cacaaatttt ctgttcgtgg tgaaggcgaa ggtgatgcaa
      121 ccaacggtaa actgaccctg aaatttatct gtaccactgg taaactgcca gttccatggc
      181 caactctggt taccactctg acctacggtg ttcaatgttt tgcacgttac ccagatcaca
      241 tgaaacaaca cgattttttc aaaagcgcaa tgccagaagg ttacgttcaa gaacgtacca
      301 tctcttttaa agatgacggc acctacaaaa cccgtgcgga agttaaattt gaaggtgata
      361 ccctggttaa ccgcattgaa ctgaaaggca tcgattttaa agaagatggt aacatcctgg
      421 gccacaaact ggaatacaac tttaactctc acaacgtgta catcaccgca gacaaacaaa
      481 aaaacggtat caaagcgaac ttcaagatcc gtcacaacgt tgaagatggt tctgttcaac
      541 tggcagatca ctaccaacaa aacaccccaa ttggtgatgg tccagttctg ctgccagata
      601 accactacct gtctacccaa agcgttctgt ctaaagatcc aaacgaaaaa cgtgatcaca
      661 tggtgctgct ggaatttgtt accgcagcag gtattaccca cggtatggat gaactgtaca
      721 aagcagcttt gagaccactc atcgcgacta gtagcggccg ctgcagtccg gcaaaaaagg
      781 gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc
      841 aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      901 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
      961 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1021 tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1081 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1141 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1201 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1261 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1321 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1381 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1441 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1501 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1561 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1621 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1681 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1741 caatctaaag tatatatgag taaacttggt ctgacagctc gaggcttgga ttctcaccaa
     1801 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
     1861 ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc
     1921 cactcgtcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
     1981 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
     2041 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
     2101 ctggtgaaac tcacccaggg attggctgat acgaaaaaca tattctcaat aaacccttta
     2161 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
     2221 tgccggaaat cgtcgtggta ttcactccag agtgatgaaa acgtttcagt ttgctcatgg
     2281 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
     2341 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
     2401 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
     2461 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     2521 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     2581 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     2641 aagttggaac ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct aagaaaccat
     2701 tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa
     2761 aaaatcctta gctttcgcta aggatgattt ctggaattcg cggccgcttc tagagcgatg
     2821 agt
//
