LOCUS       sequence-419497-        9032 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing gRNA targeting human SMARCA4 and AAVS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9032)
  AUTHORS   Aoki K, Hyuga M, Tarumoto Y, Nishibuchi G, Ueda A, Ochi Y, Sugino S,
            Mikami T, Kobushi H, Kato I, Akahane K, Inukai T, Takaori-Kondo A, 
            Takita J, Ogawa S, Yusa K
  TITLE     Canonical BAF complex regulates the oncogenic program in human 
            T-cell acute lymphoblastic leukemia.
  JOURNAL   Blood. 2023 Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857.
  PUBMED    37922452
REFERENCE   2  (bases 1 to 9032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2023
            Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857."
COMMENT     Sequence Label: 
            pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(AAVS1)-PGKpuroBFP-W
FEATURES             Location/Qualifiers
     source          1..9032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(31..486)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(168..189)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     380..399
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     613..635
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     627..644
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     628..644
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     654..673
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        654..672
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        988..1186
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1141..1161
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1204..1384
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1431..1556
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2055..2288
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2473..2517
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2815..2932
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3389..3637
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3389..3409
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3560..3579
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        3766..4265
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3801..3824)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4198..4217
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4305..4901
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4305..4324)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4801..4821
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4920..4973
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4974..5675
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(5735..5755)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6153..6164)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6616..6633)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6622..6846
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6872..6892)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6872..6890)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6911..6927)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6911..6927)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6924..6946)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6935..6951
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6959..6965,6966..6983,6984..6989))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7004..7025
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7142..7159)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7313..7901)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7393..7412)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8072..8863,8864..8932))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8695..8714
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8933..9032,1..5))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat
       61 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
      121 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
      181 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
      241 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
      301 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
      361 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
      421 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
      481 gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
      541 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
      601 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg
      661 actcactata gggcgaattg actagtacat aacttacggt aaatggcccg cctggctgac
      721 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      781 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      841 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      901 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      961 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     1021 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     1081 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     1141 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
     1201 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     1261 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     1321 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1381 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     1441 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     1501 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     1561 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1621 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1681 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1741 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1801 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaac
     1861 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct
     1921 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     1981 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     2041 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     2101 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     2161 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     2221 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     2281 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     2341 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     2401 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     2461 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     2521 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     2581 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     2641 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     2701 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     2761 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
     2821 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2881 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     2941 ttacagggac agcagagatc cagtttggtt agtaccgggc cctacgcgtc tgcagtattt
     3001 agcatgcccc acccatctgc aaggcattct ggatagtgtc aaaacagccg gaaatcaagt
     3061 ccgtttatct caaactttag cattttggga ataaatgata tttgctatgc tggttaaatt
     3121 agattttagt taaatttcct gctgaagctc tagtacgata agcaacttga cctaagtgta
     3181 aagttgagac ttccttcagg tttatatagc ttgtgcgccg cttgggtacc tcgatcagaa
     3241 cgctgtccaa ctgtttaaga gctatgctgg aaacagcata gcaagtttaa ataaggctag
     3301 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttagccg aactgtttca
     3361 cactcacgcg tccaaggtcg ggcaggaaga gggcctattt cccatgattc cttcatattt
     3421 gcatatacga tacaaggctg ttagagagat aattagaatt aatttgactg taaacacaaa
     3481 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
     3541 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt
     3601 cttggcttta tatatcttgt ggaaaggacg aaacaccggt ccctagtggc cccactggtt
     3661 taagagctat gctggaaaca gcatagcaag tttaaataag gctagtccgt tatcaacttg
     3721 aaaaagtggc accgagtcgg tgcttttttt ggatccaatt ctaccgggta ggggaggcgc
     3781 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
     3841 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     3901 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     3961 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     4021 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     4081 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     4141 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     4201 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     4261 tttcgacctg catccatcta gatctcgagc agctgaagct taccatgacc gagtacaagc
     4321 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     4381 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     4441 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     4501 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     4561 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     4621 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     4681 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     4741 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     4801 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     4861 aaggaccgcg cacctggtgc atgacccgca agcccggtgc cggcggcggg tccggaggag
     4921 agggcagagg aagtctccta acatgcggtg acgtggagga gaatcctggc ccaatgagcg
     4981 agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc
     5041 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
     5101 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc
     5161 tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt
     5221 ccttccctga gggcttcaca tgggagagag tcaccacata cgaggacggg ggcgtgctga
     5281 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
     5341 gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct
     5401 tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga
     5461 agctcgtggg cgggagccat ctgatcgcaa acatcaagac cacatataga tccaagaaac
     5521 ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca
     5581 aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg
     5641 acctccctag caaactgggg cacaagctta attgagcggc cgctcaacct ctggattaca
     5701 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     5761 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     5821 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     5881 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     5941 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     6001 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     6061 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     6121 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     6181 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct acgccttcgc cctcagacga
     6241 gtcggatctc cctttgggcc gcctccccgc aggtaccttt aagaccaatg acttacaagg
     6301 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     6361 cccaaagaag tcaagatctg ctttttgcct gtactgggtc tctctggtta gaccagagtc
     6421 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6481 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6541 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagttt
     6601 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     6661 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     6721 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     6781 aggacagcaa gggggaggat tgggaagtca atagcaggca tgctggggat gcggtgggct
     6841 ctatgggcgg ccgttaatta agcttttgtt ccctttagtg agggttaatt gcgcgcttgg
     6901 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6961 acatacgagc cgggagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7021 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7081 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tgttccgctt
     7141 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7201 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7261 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7321 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7381 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7441 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7501 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7561 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7621 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7681 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7741 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7801 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7861 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7921 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7981 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8041 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8101 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8161 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8221 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8281 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8341 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8401 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8461 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8521 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8581 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8641 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8701 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8761 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8821 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8881 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8941 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9001 aatgtattta gaaaaataaa caaatagggg tt
//
