LOCUS       sequence-419503-        8650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing gRNA targeting human RUNX1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Aoki K, Hyuga M, Tarumoto Y, Nishibuchi G, Ueda A, Ochi Y, Sugino S,
            Mikami T, Kobushi H, Kato I, Akahane K, Inukai T, Takaori-Kondo A, 
            Takita J, Ogawa S, Yusa K
  TITLE     Canonical BAF complex regulates the oncogenic program in human 
            T-cell acute lymphoblastic leukemia.
  JOURNAL   Blood. 2023 Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857.
  PUBMED    37922452
REFERENCE   2  (bases 1 to 8650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2023
            Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857."
COMMENT     Sequence Label: pKLV2-U6gRNA5(RUNX1(11))-PGKpuro2ABFP-W
FEATURES             Location/Qualifiers
     source          1..8650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        230..428
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     383..403
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             446..626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    673..798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1297..1530
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1715..1759
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2057..2174
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2249..2497
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2249..2269
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2420..2439
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2626..3125
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2661..2684)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3058..3077
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3165..3761
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3165..3184)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3661..3681
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3780..3833
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3834..4535
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(4595..4615)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5013..5024)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5476..5493)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5482..5706
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5732..5752)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5732..5750)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5771..5787)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5771..5787)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5784..5806)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5795..5811
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5819..5825,5826..5843,5844..5849))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5864..5885
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6002..6019)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6173..6761)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6253..6272)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6932..7723,7724..7792))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7555..7574
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7793..7897)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7923..8378)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8060..8081)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8272..8291
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8505..8527
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8519..8536
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8520..8536
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8546..8565
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8546..8564
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
       61 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      121 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      181 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      241 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      301 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      361 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      421 ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
      481 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
      541 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
      601 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
      661 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
      721 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
      781 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
      841 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
      901 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
      961 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     1021 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     1081 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     1141 ggccgcgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     1201 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     1261 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     1321 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     1381 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     1441 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     1501 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     1561 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     1621 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     1681 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     1741 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     1801 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     1861 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     1921 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     1981 cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg
     2041 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     2101 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     2161 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg ttagtaccgg
     2221 gccctacgcg tccaaggtcg ggcaggaaga gggcctattt cccatgattc cttcatattt
     2281 gcatatacga tacaaggctg ttagagagat aattagaatt aatttgactg taaacacaaa
     2341 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
     2401 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt
     2461 cttggcttta tatatcttgt ggaaaggacg aaacaccggt aggtggcgac ttgcggtgtt
     2521 taagagctat gctggaaaca gcatagcaag tttaaataag gctagtccgt tatcaacttg
     2581 aaaaagtggc accgagtcgg tgcttttttt ggatccaatt ctaccgggta ggggaggcgc
     2641 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
     2701 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     2761 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     2821 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     2881 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     2941 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     3001 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     3061 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     3121 tttcgacctg catccatcta gatctcgagc agctgaagct taccatgacc gagtacaagc
     3181 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     3241 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     3301 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     3361 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     3421 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     3481 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     3541 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     3601 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     3661 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     3721 aaggaccgcg cacctggtgc atgacccgca agcccggtgc cggcggcggg tccggaggag
     3781 agggcagagg aagtctccta acatgcggtg acgtggagga gaatcctggc ccaatgagcg
     3841 agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc
     3901 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
     3961 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc
     4021 tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt
     4081 ccttccctga gggcttcaca tgggagagag tcaccacata cgaggacggg ggcgtgctga
     4141 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
     4201 gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct
     4261 tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga
     4321 agctcgtggg cgggagccat ctgatcgcaa acatcaagac cacatataga tccaagaaac
     4381 ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca
     4441 aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg
     4501 acctccctag caaactgggg cacaagctta attgagcggc cgctcaacct ctggattaca
     4561 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4621 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4681 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     4741 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     4801 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     4861 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     4921 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     4981 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5041 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct acgccttcgc cctcagacga
     5101 gtcggatctc cctttgggcc gcctccccgc aggtaccttt aagaccaatg acttacaagg
     5161 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     5221 cccaaagaag tcaagatctg ctttttgcct gtactgggtc tctctggtta gaccagagtc
     5281 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5341 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5401 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagttt
     5461 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     5521 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     5581 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     5641 aggacagcaa gggggaggat tgggaagtca atagcaggca tgctggggat gcggtgggct
     5701 ctatgggcgg ccgttaatta agcttttgtt ccctttagtg agggttaatt gcgcgcttgg
     5761 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     5821 acatacgagc cgggagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     5881 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     5941 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     6001 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6061 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     6121 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6181 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     6241 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     6301 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     6361 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     6421 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     6481 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     6541 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     6601 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     6661 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     6721 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     6781 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     6841 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     6901 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     6961 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     7021 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     7081 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     7141 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     7201 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     7261 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     7321 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     7381 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     7441 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     7501 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     7561 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     7621 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     7681 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     7741 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     7801 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     7861 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     7921 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     7981 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     8041 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     8101 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     8161 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     8221 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     8281 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     8341 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
     8401 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     8461 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     8521 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgactagtac
     8581 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     8641 aataatgacg
//
