LOCUS       sequence-419548-       10061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible lentiviral vector for FOXM1b expression in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Barger CJ, Zhang W, Hillman J, Stablewski AB, Higgins MJ, 
            Vanderhyden BC, Odunsi K, Karpf AR
  TITLE     Genetic determinants of FOXM1 overexpression in epithelial ovarian 
            cancer and functional contribution to cell cycle progression.
  JOURNAL   Oncotarget. 2015 Jul 16.
  PUBMED    26243836
REFERENCE   2  (bases 1 to 10061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncotarget.
            2015 Jul 16."
COMMENT     Sequence Label: pCW57.1-FOXM1b
FEATURES             Location/Qualifiers
     source          1..10061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     33..50
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    167..188
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(203..208,209..226,227..233)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    241..257
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     246..268
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     265..281
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     265..281
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     300..320
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        302..320
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        348..574
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             575..755
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    802..927
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1420..1653
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1838..1882
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2095..2212
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2266..2580
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2274..2292
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2310..2328
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2345..2363
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2381..2399
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2417..2435
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2452..2470
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2488..2506
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2553..2577
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2601..2625
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2673..2699
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     RBS             3987..3998
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    complement(4988..5012)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5030..5047
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        5057..5567
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5486..5504
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5577..6173
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5577..5596)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6073..6093
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6174..6227
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6228..6845,6846..6851,6852..6887,6888..6890,6891..6926,
                     6927..6929,6930..6965,6966..6974)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7014..7034)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7432..7443)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7648..7881
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7953..8074
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7990..8009)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8044..8063
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8114..8249
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8249..8320
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8321..8389,8390..9181)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8539..8558)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9352..9940
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9841..9860
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
       61 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
      121 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      181 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      241 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      301 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
      361 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
      421 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
      481 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
      541 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
      601 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      661 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      721 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      781 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      841 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      901 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      961 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     1021 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     1081 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1141 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1201 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1261 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1321 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1381 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1441 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1501 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1561 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1621 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1681 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1741 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1801 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1861 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1921 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1981 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     2041 aaccccgagg ggacaattct cgacctcgag acaaatggca gtattcatcc acaattttaa
     2101 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2161 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     2221 ttacagggac agcagagatc cactttggcc gcgaatcgat atgtcgagtt tactccctat
     2281 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt
     2341 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
     2401 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
     2461 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
     2521 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2581 tggagaattg gctagcatca acaagtttgt acaaaaaagc aggctcggga attcgtcgac
     2641 tggatccggt accgaggaga tctgccacca tgtacccata cgatgttcca gattacgctg
     2701 cgatcgccgg cgcgccgaaa actagccccc gtcggccact gattctcaaa agacggaggc
     2761 tgccccttcc tgttcaaaat gccccaagtg aaacatcaga ggaggaacct aagagatccc
     2821 ctgcccaaca ggagtctaat caagcagagg cctccaagga agtggcagag tccaactctt
     2881 gcaagtttcc agctgggatc aagattatta accaccccac catgcccaac acgcaagtag
     2941 tggccatccc caacaatgct aatattcaca gcatcatcac agcactgact gccaagggaa
     3001 aagagagtgg cagtagtggg cccaacaaat tcatcctcat cagctgtggg ggagccccaa
     3061 ctcagcctcc aggactccgg cctcaaaccc aaaccagcta tgatgccaaa aggacagaag
     3121 tgaccctgga gaccttggga ccaaaacctg cagctaggga tgtgaatctt cctagaccac
     3181 ctggagccct ttgcgagcag aaacgggaga cctgtgcaga tggtgaggca gcaggctgca
     3241 ctatcaacaa tagcctatcc aacatccagt ggcttcgaaa gatgagttct gatggactgg
     3301 gctcccgcag catcaagcaa gagatggagg aaaaggagaa ttgtcacctg gagcagcgac
     3361 aggttaaggt tgaggagcct tcgagaccat cagcgtcctg gcagaactct gtgtctgagc
     3421 ggccacccta ctcttacatg gccatgatac aattcgccat caacagcact gagaggaagc
     3481 gcatgacttt gaaagacatc tatacgtgga ttgaggacca ctttccctac tttaagcaca
     3541 ttgccaagcc aggctggaag aactccatcc gccacaacct ttccctgcac gacatgtttg
     3601 tccgggagac gtctgccaat ggcaaggtct ccttctggac cattcacccc agtgccaacc
     3661 gctacttgac attggaccag gtgtttaagc agcagaaacg accgaatcca gagctccgcc
     3721 ggaacatgac catcaaaacc gaactccccc tgggcgcacg gcggaagatg aagccactgc
     3781 taccacgggt cagctcatac ctggtaccta tccagttccc ggtgaaccag tcactggtgt
     3841 tgcagccctc ggtgaaggtg ccattgcccc tggcggcttc cctcatgagc tcagagcttg
     3901 cccgccatag caagcgagtc cgcattgccc ccaaggtgct gctagctgag gaggggatag
     3961 ctcctctttc ttctgcagga ccagggaaag aggagaaact cctgtttgga gaagggtttt
     4021 ctcctttgct tccagttcag actatcaagg aggaagaaat ccagcctggg gaggaaatgc
     4081 cacacttagc gagacccatc aaagtggaga gccctccctt ggaagagtgg ccctccccgg
     4141 ccccatcttt caaagaggaa tcatctcact cctgggagga ttcgtcccaa tctcccaccc
     4201 caagacccaa gaagtcctac agtgggctta ggtccccaac ccggtgtgtc tcggaaatgc
     4261 ttgtgattca acacagggag aggagggaga ggagccggtc tcggaggaaa cagcatctac
     4321 tgcctccctg tgtggatgag ccggagctgc tcttctcaga ggggcccagt acttcccgct
     4381 gggccgcaga gctcccgttc ccagcagact cctctgaccc tgcctcccag ctcagctact
     4441 cccaggaagt gggaggacct tttaagacac ccattaagga aacgctgccc atctcctcca
     4501 ccccgagcaa atctgtcctc cccagaaccc ctgaatcctg gaggctcacg cccccagcca
     4561 aagtaggggg actggatttc agcccagtac aaacccccca gggtgcctct gaccccttgc
     4621 ctgaccccct ggggctgatg gatctcagca ccactccctt gcaaagtgct cccccccttg
     4681 aatcaccgca aaggctcctc agttcagaac ccttagacct catctccgtc ccctttggca
     4741 actcttctcc ctcagatata gacgtcccca agccaggctc cccggagcca caggtttctg
     4801 gccttgcagc caatcgttct ctgacagaag gcctggtcct ggacacaatg aatgacagcc
     4861 tcagcaagat cctgctggac atcagctttc ctggcctgga cgaggaccca ctgggccctg
     4921 acaacatcaa ctggtcccag tttattcctg agctacagta gagcccctcg aggtttaaac
     4981 ggccggcacc cagctttctt gtacaaagtg gtttagtaat gaaccggtcc accaccacca
     5041 ccaccactaa ggatccgggg ttggggttgc gccttttcca aggcagccct gggtttgcgc
     5101 agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc
     5161 gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc
     5221 ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt
     5281 gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc
     5341 agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag
     5401 ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag
     5461 tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt
     5521 cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagcaa ttcaccatga
     5581 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     5641 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     5701 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     5761 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     5821 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     5881 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     5941 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     6001 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     6061 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     6121 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccgaaggta
     6181 gaggttctct cctcacttgt ggtgatgttg aagaaaaccc tggtccaatg tctagactgg
     6241 acaagagcaa agtcataaac ggagctctgg aattactcaa tggtgtcggt atcgaaggcc
     6301 tgacgacaag gaaactcgct caaaagctgg gagttgagca gcctaccctg tactggcacg
     6361 tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga gatgctggac aggcatcata
     6421 cccacttctg ccccctggaa ggcgagtcat ggcaagactt tctgcggaac aacgccaagt
     6481 cataccgctg tgctctcctc tcacatcgcg acggggctaa agtgcatctc ggcacccgcc
     6541 caacagagaa acagtacgaa accctggaaa atcagctcgc gttcctgtgt cagcaaggct
     6601 tctccctgga gaacgcactg tacgctctgt ccgccgtggg ccactttaca ctgggctgcg
     6661 tattggagga acaggagcat caagtagcaa aagaggaaag agagacacct accaccgatt
     6721 ctatgccccc acttctgaga caagcaattg agctgttcga ccggcaggga gccgaacctg
     6781 ccttcctttt cggcctggaa ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa
     6841 gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga catgctccca gccgatgccc
     6901 ttgacgactt tgaccttgat atgctgcctg ctgacgctct tgacgatttt gaccttgaca
     6961 tgctccccgg gtaactaagt aaggatcgat ccaagatatc gtattcttaa ctatgttgct
     7021 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7081 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7141 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7201 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7261 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     7321 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     7381 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7441 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     7501 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgtttcgcct
     7561 cggcgtccgg actagaggta cctttaagac caatgactta caaggcagct gtagatctta
     7621 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     7681 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7741 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7801 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7861 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     7921 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     7981 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     8041 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     8101 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     8161 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     8221 gaagtagtga ggaggctttt ttggaggcct ttcaaatatg tatccgctca tgagacaata
     8281 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     8341 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     8401 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     8461 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     8521 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     8581 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     8641 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     8701 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     8761 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     8821 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     8881 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     8941 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     9001 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     9061 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     9121 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     9181 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     9241 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     9301 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     9361 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     9421 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     9481 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     9541 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     9601 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     9661 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     9721 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     9781 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     9841 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     9901 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     9961 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    10021 tgattctgtg gataaccgta ttaccgcctt tgagtgagct g
//
