LOCUS       sequence-419561-        5086 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pGL3 promoter luciferase reporter vector containing 3 copies of the 
            NurRE POMC promoter DNA response element 
            (5′-GATCGTGATATTTACCTCCAAATGCCA- 3′) used for luciferase reporter 
            assays in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Munoz-Tello P, Lin H, Khan P, de Vera IMS, Kamenecka TM, Kojetin DJ
  TITLE     Assessment of NR4A Ligands That Directly Bind and Modulate the 
            Orphan Nuclear Receptor Nurr1.
  JOURNAL   J Med Chem. 2020 Dec 24;63(24):15639-15654. doi: 
            10.1021/acs.jmedchem.0c00894. Epub 2020 Dec 8.
  PUBMED    33289551
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.0c00894"; journalName: "J Med Chem"; date: 
            "2020-12-24- 24"; volume: "63"; issue: "24"; pages: "15639-15654"
COMMENT     Sequence Label: pGL3-NurRE3
FEATURES             Location/Qualifiers
     source          1..5086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(435..1226,1227..1295))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1058..1077
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1296..1400)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1427..1882
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1514..1533)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1724..1745
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    2013..2061
                     /note="synthetic polyadenylation signal"
     misc_feature    2075..2166
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2115..2134
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        2297..2493
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2344..2479
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      2523..2532
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2529..4181
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2595..2613)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    4222..4343
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4233..4252)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4287..4306
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4591..4608)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(4762..5086,1..264))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4842..4861)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
       61 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
      121 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      181 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      241 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      301 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      361 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
      421 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
      481 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
      541 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
      601 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
      661 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
      721 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
      781 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
      841 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
      901 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
      961 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1021 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     1081 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1141 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1201 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1261 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1321 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1381 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc
     1441 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     1501 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     1561 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     1621 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     1681 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     1741 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     1801 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     1861 aaaatattaa cgcttacaat ttgccattcg ccattcaggc tgcgcaactg ttgggaaggg
     1921 cgatcggtgc gggcctcttc gctattacgc cagcccaagc taccatgata agtaagtaat
     1981 attaaggtac gggaggtact tggagcggcc gcaataaaat atctttattt tcattacatc
     2041 tgtgtgttgg ttttttgtgt gaatcgatag tactaacata cgctctccat caaaacaaaa
     2101 cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat
     2161 ttctctatcg ataggtaccg agctcttacg cgtgctagcg atcgtgatat ttacctccaa
     2221 atgccagatc gtgatattta cctccaaatg ccagatcgtg atatttacct ccaaatgcca
     2281 ctcgagatct gcgatctgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     2341 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatcg ctgactaatt
     2401 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     2461 ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gcattccggt actgttggta
     2521 aagccaccat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg
     2581 aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg
     2641 gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg
     2701 aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa
     2761 tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta
     2821 tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta
     2881 tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga
     2941 acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct aaaacggatt
     3001 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg
     3061 aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact
     3121 cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga
     3181 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt
     3241 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat
     3301 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc
     3361 aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa
     3421 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc
     3481 ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc
     3541 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata
     3601 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata
     3661 ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta
     3721 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc
     3781 tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc
     3841 tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct
     3901 tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg
     3961 gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga
     4021 tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt
     4081 ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga
     4141 tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta attctagagt cggggcggcc
     4201 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     4261 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     4321 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     4381 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg
     4441 ataaggatcc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     4501 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     4561 ggacaggtgc cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     4621 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     4681 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     4741 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4801 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4861 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4921 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4981 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     5041 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggta
//
