LOCUS       sequence-419572-        9088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens. This is the control vector 
            for the collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9088)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3549
FEATURES             Location/Qualifiers
     source          1..9088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             382..426
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        657..867
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        679..875
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      726..861
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             907..1509
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(907..926)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1403..1423
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1552..1669
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1807..2985
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2038..2976
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2933..2953
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      2996..3005
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3002..3004,3005..3007,3008..3724)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKS"
     primer_bind     complement(3047..3068)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3308..3327)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3655..3676
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3832..4420
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3885..3905)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4303..4314)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4492..4725
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4803..4924
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4840..4859)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4894..4913
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4964..5099
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5119..5138)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5120..5138)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5148..5165)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5148..5164)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5157..5179)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5306..5761
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5393..5412)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5603..5624
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5787..5891
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5892..5960,5961..6752)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6110..6129)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6923..7511
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7412..7431
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7665..7682
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7799..7820
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7835..7840,7841..7858,7859..7865)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7873..7889
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7878..7900
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7897..7913
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7897..7913
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7932..7952
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7934..7952
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7980..8206
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8207..8387
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8434..8559
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    join(9052..9088,1..197)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
       61 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
      121 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
      181 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
      241 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
      301 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
      361 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
      421 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
      481 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
      541 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
      601 atgcattgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
      661 gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac catagtcccg cccctaactc
      721 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
      781 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
      841 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttctagaggt accaccggag
      901 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
      961 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     1021 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     1081 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg
     1141 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     1201 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     1261 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     1321 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     1381 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     1441 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     1501 gccagttaac gaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag
     1561 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     1621 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     1681 cagggacagc agagatccac tttggcgccg gctcgaggcc tgcaggtgca aagatggata
     1741 aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg
     1801 ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     1861 ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg
     1921 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     1981 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta
     2041 agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct
     2101 tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2161 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2221 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2281 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2341 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2401 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2461 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2521 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     2581 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     2641 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     2701 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     2761 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     2821 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     2881 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     2941 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaggcta gcaccgccac
     3001 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     3061 cggcgacgta aacggccaca agttcagcgt gtctggcgag ggcgagggcg atgccaccta
     3121 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     3181 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     3241 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     3301 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3361 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     3421 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     3481 cggcatcaag gcgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     3541 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3601 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     3661 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc
     3721 ctagtaatga actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     3781 tggaaaggac gagctgactc cttcttggga atctgcacgc gttaagtcga caatcaacct
     3841 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     3901 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     3961 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4021 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4081 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4141 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4201 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     4261 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4321 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4381 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     4441 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     4501 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     4561 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     4621 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     4681 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     4741 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     4801 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     4861 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     4921 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     4981 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     5041 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     5101 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     5161 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     5221 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     5281 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5341 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     5401 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5461 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5521 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     5581 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     5641 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     5701 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     5761 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5821 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5881 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5941 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6001 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6061 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6121 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6181 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6241 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6301 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6361 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6421 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6481 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6541 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6601 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6661 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6721 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6781 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6841 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6901 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6961 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7021 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     7081 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7141 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7201 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7261 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7321 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7381 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7441 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7501 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7561 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7621 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7681 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7741 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     7801 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     7861 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     7921 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     7981 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     8041 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     8101 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     8161 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     8221 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8281 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8341 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     8401 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     8461 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     8521 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     8581 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     8641 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     8701 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     8761 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     8821 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     8881 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     8941 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     9001 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     9061 gttccttggg ttcttgggag cagcagga
//
