LOCUS       sequence-419573-        8694 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Soybean APEX2 anchored to cytosolic face of ER membrane with 
            N-terminal targeting sequence of residues 1-27 of ER-resident P450 
            oxidase 2C1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, 
            Ting AY
  TITLE     Directed evolution of APEX2 for electron microscopy and proximity 
            labeling.
  JOURNAL   Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
  PUBMED    25419960
REFERENCE   2  (bases 1 to 8694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179."
COMMENT     Sequence Label: ERM-APEX2
FEATURES             Location/Qualifiers
     source          1..8694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(261..272)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             450..683
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    761..882
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(798..817)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     852..871
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      922..1057
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1077..1096)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1078..1096)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1106..1123)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1106..1122)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1115..1137)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1264..1719
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1351..1370)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1561..1582
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1745..1849
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1850..1918,1919..2710)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2068..2087)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2881..3469
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3370..3389
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3623..3640
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3757..3778
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3793..3798,3799..3816,3817..3823)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3831..3847
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3836..3858
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3855..3871
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3855..3871
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3890..3910
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3892..3910
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3938..4164
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4165..4345
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4392..4517
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5010..5243
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5428..5472
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5647..6147
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6066..6084
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6159..6554
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    6614..6731
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6848..7151
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7152..7355
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7306..7326
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    7378..7402
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7579..8325
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             8326..8367
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(8375..8399)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8401..8442
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    join(8484..8694,1..378)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8537..8557)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
       61 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
      121 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
      181 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
      241 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
      301 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
      361 tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct
      421 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
      481 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
      541 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
      601 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
      661 gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta
      721 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
      781 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
      841 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
      901 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      961 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     1021 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     1081 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     1141 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     1201 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     1261 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     1321 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     1381 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     1441 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     1501 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     1561 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     1621 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     1681 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     1741 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     1801 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     1861 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     1921 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     1981 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     2041 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     2101 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     2161 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     2221 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     2281 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     2341 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     2401 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     2461 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     2521 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     2581 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     2641 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     2701 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     2761 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     2821 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2881 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2941 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3001 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3061 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3121 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3181 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3241 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3301 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3361 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3421 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3481 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3541 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     3601 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     3661 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     3721 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     3781 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     3841 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     3901 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     3961 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     4021 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     4081 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     4141 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     4201 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     4261 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     4321 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     4381 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     4441 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     4501 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     4561 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     4621 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     4681 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     4741 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     4801 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     4861 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     4921 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     4981 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     5041 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     5101 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     5161 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     5221 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     5281 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     5341 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     5401 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     5461 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     5521 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     5581 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     5641 ggacccttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     5701 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     5761 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     5821 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     5881 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     5941 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     6001 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     6061 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     6121 gaccgaatca ccgacctctc tccccagggg gtaccaccat ggccaagcct ttgtctcaag
     6181 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag
     6241 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg
     6301 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg
     6361 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga
     6421 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag
     6481 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt
     6541 atgtgtggga gggcctgcag ctgcagtagt aagaattcta gatcttgaga caaatggcag
     6601 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     6661 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     6721 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg
     6781 gggcccgggg ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     6841 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     6901 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     6961 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     7021 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     7081 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     7141 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     7201 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     7261 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     7321 ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactgtc gggatcaaca
     7381 agtttgtaca aaaaagcagg cttcgaagga gatagaacca tggaccctgt ggtcgtgctg
     7441 gggctgtgcc tgagctgtct gctgctgctg agcctgtgga agcagtccta cggaggcggg
     7501 ggatcaggaa gcggcggccg cggatcagga agcggatcag gaagcggatc aggaagcgga
     7561 tcaggaagcg gatcaggagg aaagtcttac ccaactgtga gtgctgatta ccaggacgcc
     7621 gttgagaagg cgaagaagaa gctcagaggc ttcatcgctg agaagagatg cgctcctcta
     7681 atgctccgtt tggcattcca ctctgctgga acctttgaca agggcacgaa gaccggtgga
     7741 cccttcggaa ccatcaagca ccctgccgaa ctggctcaca gcgctaacaa cggtcttgac
     7801 atcgctgtta ggcttttgga gccactcaag gcggagttcc ctattttgag ctacgccgat
     7861 ttctaccagt tggctggcgt tgttgccgtt gaggtcacgg gtggacctaa ggttccattc
     7921 caccctggaa gagaggacaa gcctgagcca ccaccagagg gtcgcttgcc cgatcccact
     7981 aagggttctg accatttgag agatgtgttt ggcaaagcta tggggcttac tgaccaagat
     8041 atcgttgctc tatctggggg tcacactatt ggagctgcac acaaggagcg ttctggattt
     8101 gagggtccct ggacctctaa tcctcttatt ttcgacaact catacttcac ggagttgttg
     8161 agtggtgaga aggaaggtct ccttcagcta ccttctgaca aggctctttt gtctgaccct
     8221 gtattccgcc ctctcgttga caaatatgca gcggacgaag atgccttctt tgctgattac
     8281 gctgaggctc accaaaagct ttccgagctt gggtttgctg atgccggcaa gcccatcccc
     8341 aaccccctgc tgggcctgga cagcacctga taagacccag ctttcttgta caaagtggtt
     8401 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct
     8461 aaccggtggc gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     8521 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     8581 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     8641 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgt
//
