LOCUS       sequence-419576-        5373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Drecktrah D, Levine-Wilkinson S, Dam T, Winfree S, Knodler LA, 
            Schroer TA, Steele-Mortimer O
  TITLE     Dynamic behavior of Salmonella-induced membrane tubules in 
            epithelial cells.
  JOURNAL   Traffic. 2008 Dec . 9(12):2117-29.
  PUBMED    18785994
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic. 
            2008 Dec . 9(12):2117-29."
COMMENT     Sequence Label: pFPV-mCherry
FEATURES             Location/Qualifiers
     source          1..5373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     312..331
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     565..582
                     /label=L4440
                     /note="L4440 vector, forward primer"
     oriT            1895..1982
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             complement(2596..3306)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2874..2893)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3143..3161
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(3974..3992)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4060..4164
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4165..4233,4234..5025)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4383..4402)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(5196..5373,1..411)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
       61 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
      121 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
      181 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
      241 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      301 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      361 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      421 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      481 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
      541 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgctca
      601 cagcccatgg ccttgagctg tcggcctatg gcctgcaaag tcctgtcgtt cttcatcggg
      661 ccaccaagcg cagccagatc gagccgtcct cggttgtcag tggcgtcagg tcgagcaaga
      721 gcaacgatgc gatcagcagc accaccgtag gcatcatgga agccagcatc acggttagcc
      781 atagcttcca gtgccacccc cgcgacgcgc tccgggcgct ctgcgcggcg ctgctcacct
      841 cggcggctac ctcccgcaac tctttggcca gctccaccca tgccgcccct gtctggcgct
      901 gggctttcag ccactccgcc gcctgcgcct cgctggcctg ctgggtctgg ctcatgacct
      961 gccgggcttc gtcggccagt gtcgccatgc tctgggccag cggttcgatc tgctccgcta
     1021 actcgttgat gcctctggat ttcttcactc tgtcgattgc gttcatggtc tattgcctcc
     1081 cggtattcct gtaagtcgat gatctgggcg ttggcggtgt cgatgttcag ggccacgtct
     1141 gcccggtcgg tgcggatgcc ccggccttcc atctccacca cgttcggccc caggtgaaca
     1201 ccgggcaggc gctcgatgcc ctgcgcctca agtgttctgt ggtcaatgcg ggcgtcgtgg
     1261 ccagcccgct ctaatgcccg gttggcatgg tcggcccatg cctcgcgggt ctgctcaagc
     1321 catgccttgg gcttgagcgc ttcggtcttc tgtgccccgc ccttctccgg ggtcttgccg
     1381 ttgtaccgct tgaaccactg agcggcgggc cgctcgatgc cgtcattgat ccgctcggag
     1441 atcatcaggt ggcagtgcgg gttctcgccg ccaccggcat ggatggccag cgtatacggc
     1501 aggcgctcgg caccggtcag gtgctgggcg aactcggacg ccagcgcctt ctgctggtcg
     1561 agggtcagct cgaccggcag ggcaaattcg acctccttga acagccgccc attggcgcgt
     1621 tcatacaggt cggcagcatc ccagtagtcg gcgggccgct cgacgaactc cggcatgtgc
     1681 ccggattcgg cgtgcaagac ttcatccatg tcgcgggcat acttgccttc gcgctggatg
     1741 tagtcggcct tggccctggc cgattggccg cccgacctgc tgccggtttt cgccgtaagg
     1801 tgataaatcg ccatgctgcc tcgctgttgc ttttgctttt cggctccatg caatggccct
     1861 cggagagcgc accgcccgaa gggtggccgt taggccagtt tctcgaagag aaaccggtaa
     1921 gtgcgccctc ccctacaaag tagggtcggg attgccgccg ctgtgcctcc atgatagcct
     1981 acgagacagc acattaacaa tggggtgtca agatggttaa ggggagcaac aaggcggcgg
     2041 atcggctggc caagctcgaa gaacaacgag cgcgaatcaa tgccgaaatt cagcgggtgc
     2101 gggcaaggga acagcagcaa gagcgcaaga acgaaacaag gcgcaaggtg ctggtggggg
     2161 ccatgatttt ggccaaggtg aacagcagcg agtggccgga ggatcggctc atggcggcaa
     2221 tggatgcgta ccttgaacgc gaccacgacc gcgccttgtt cggtctgccg ccacgccaga
     2281 aggatgagcc gggctgaatg atcgaccgag acaggccctg cggggctgca cacgcgcccc
     2341 cacccttcgg gtagggggaa aggccgctaa agcggctaaa agcgctagca gcacgccata
     2401 gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat
     2461 aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt
     2521 gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca
     2581 ttaaagcttg catgcttact tgtacagctc gtccatgccg ccggtggagt ggcggccctc
     2641 ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccaactt
     2701 gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt aggtggtctt
     2761 gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga tctcgccctt
     2821 cagggcgccg tcctcggggt acatccgctc ggaggaggcc tcccagccca tggtcttctt
     2881 ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct tgtagatgaa
     2941 ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt
     3001 catcacgcgc tcccacttga agccctcggg gaaggacagc ttcaagtagt cggggatgtc
     3061 ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac tgaggggaca ggatgtccca
     3121 ggcgaagggc agggggccac ccttggtcac cttcagcttg gcggtctggg tgccctcgta
     3181 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat
     3241 gtgcaccttg aagcgcatga actccttgat gatggccatg ttatcctcct cgcccttgct
     3301 caccatatgt atatctcctt cttaaatcta gaggatctta acattttcag cgatacccgc
     3361 tgcagccagg atggctttag aacgggtctt gccgacaccg tagatcgaag ttaacgcgat
     3421 cacggcgtgt ttctgatcag gaatgttaat gcctgctata cgggccacta tgcactccta
     3481 ctatttaata tgtacgtacc atgctgaaaa gcccgttttc aggatactca aatggaaacg
     3541 tacagacata caaaagattg gctggctaat ctagccagct caacccaact ttgcaagaaa
     3601 aatatgcgaa aaaatcagcc ttggcgctgt ttatgcttcg gctcggcact gcaaatcaca
     3661 cggatgacac catcacgctt aacgattttg cagttacggc ataatttctt gacggaagca
     3721 cgaactttca tttttactct ccgtaacttc tcaggcgacc aattaacggc cgtagccttt
     3781 caggttcgcc ttcttcaatg cagactcata ctgactggac atcatcagag tttgcacttg
     3841 agccataaag tccataatca cgacaacaac gataagcagt gaggtcccac cgaagtagaa
     3901 cggcactttc attgcatcac gcatgaactc cgggatcccc gggtaccgag ctcgaattct
     3961 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     4021 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4081 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4141 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4201 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4261 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4321 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4381 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     4441 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4501 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4561 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4621 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4681 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
     4741 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4801 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4861 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4921 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     4981 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5041 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5101 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5161 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5221 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5281 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5341 gtccttctag tgtagccgta gttaggccac cac
//
