LOCUS       sequence-419580-       11521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of GFP using a self-replicating Venezuelan equine 
            encephalitis (VEE) virus RNA replicon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, 
            Chi NC, Fu XD, Yu BD, Dowdy SF
  TITLE     Efficient generation of human iPSCs by a synthetic self-replicative 
            RNA.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 
            10.1016/j.stem.2013.06.001.
  PUBMED    23910086
REFERENCE   2  (bases 1 to 11521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.06"; journalName: "Cell Stem Cell"; date: 
            "2013-08-1- 1"; volume: "13"; issue: "2"; pages: "246-54"
COMMENT     Sequence Label: T7-VEE-GFP
FEATURES             Location/Qualifiers
     source          1..11521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1863..1872
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1869..1871,1872..1874,1875..2588)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1869..1871,1872..1874,1875..2585)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1914..1935)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2175..2194)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2522..2543
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2673..3222
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2817..2834)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3044..3063
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3225..3824
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3225..3244)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3721..3741
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     promoter        4022..4126
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4127..4195,4196..4987)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4345..4364)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5158..5746
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5647..5666
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     RBS             7319..7327
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 acaacaaaaa tcagtaaggc aaacggtgct atccgaagtg gtgttggaga ggaccgaatt
       61 ggagatttcg tatgccccgc gcctcgacca agaaaaagaa gaattactac gcaagaaatt
      121 acagttaaat cccacacctg ctaacagaag cagataccag tccaggaagg tggagaacat
      181 gaaagccata acagctagac gtattctgca aggcctaggg cattatttga aggcagaagg
      241 aaaagtggag tgctaccgaa ccctgcatcc tgttcctttg tattcatcta gtgtgaaccg
      301 tgccttttca agccccaagg tcgcagtgga agcctgtaac gccatgttga aagagaactt
      361 tccgactgtg gcttcttact gtattattcc agagtacgat gcctatttgg acatggttga
      421 cggagcttca tgctgcttag acactgccag tttttgccct gcaaagctgc gcagctttcc
      481 aaagaaacac tcctatttgg aacccacaat acgatcggca gtgccttcag cgatccagaa
      541 cacgctccag aacgtcctgg cagctgccac aaaaagaaat tgcaatgtca cgcaaatgag
      601 agaattgccc gtattggatt cggcggcctt taatgtggaa tgcttcaaga aatatgcgtg
      661 taataatgaa tattgggaaa cgtttaaaga aaaccccatc aggcttactg aagaaaacgt
      721 ggtaaattac attaccaaat taaaaggacc aaaagctgct gctctttttg cgaagacaca
      781 taatttgaat atgttgcagg acataccaat ggacaggttt gtaatggact taaagagaga
      841 cgtgaaagtg actccaggaa caaaacatac tgaagaacgg cccaaggtac aggtgatcca
      901 ggctgccgat ccgctagcaa cagcgtatct gtgcggaatc caccgagagc tggttaggag
      961 attaaatgcg gtcctgcttc cgaacattca tacactgttt gatatgtcgg ctgaagactt
     1021 tgacgctatt atagccgagc acttccagcc tggggattgt gttctggaaa ctgacatcgc
     1081 gtcgtttgat aaaagtgagg acgacgccat ggctctgacc gcgttaatga ttctggaaga
     1141 cttaggtgtg gacgcagagc tgttgacgct gattgaggcg gctttcggcg aaatttcatc
     1201 aatacatttg cccactaaaa ctaaatttaa attcggagcc atgatgaaat ctggaatgtt
     1261 cctcacactg tttgtgaaca cagtcattaa cattgtaatc gcaagcagag tgttgagaga
     1321 acggctaacc ggatcaccat gtgcagcatt cattggagat gacaatatcg tgaaaggagt
     1381 caaatcggac aaattaatgg cagacaggtg cgccacctgg ttgaatatgg aagtcaagat
     1441 tatagatgct gtggtgggcg agaaagcgcc ttatttctgt ggagggttta ttttgtgtga
     1501 ctccgtgacc ggcacagcgt gccgtgtggc agacccccta aaaaggctgt ttaagcttgg
     1561 caaacctctg gcagcagacg atgaacatga tgatgacagg agaagggcat tgcatgaaga
     1621 gtcaacacgc tggaaccgag tgggtattct ttcagagctg tgcaaggcag tagaatcaag
     1681 gtatgaaacc gtaggaactt ccatcatagt tatggccatg actactctag ctagcagtgt
     1741 taaatcattc agctacctga gaggggcccc tataactctc tacggctaac ctgaatggac
     1801 tacgacatag tctagtccgc caagtctagc atatgggcgc gccctcagca tcgattcaat
     1861 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1921 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1981 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     2041 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     2101 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     2161 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     2221 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     2281 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     2341 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     2401 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     2461 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     2521 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     2581 acaagtagtc tagagtcgac ccgggcggcc gcaactaact taagctagca acggtttccc
     2641 tctagcggga tcaattccgc cccccccccc taacgttact ggccgaagcc gcttggaata
     2701 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     2761 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     2821 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     2881 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     2941 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     3001 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     3061 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     3121 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     3181 gaaccacggg gacgtggttt tcctttgaaa aacacgataa taccatgacc gagtacaagc
     3241 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     3301 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     3361 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     3421 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     3481 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     3541 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     3601 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     3661 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     3721 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     3781 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgagaattg gcaagctgct
     3841 tacatagaac tcgcggcgat tggcatgccg ccttaaaatt tttattttat tttttctttt
     3901 cttttccgaa tcggattttg tttttaatat ttcaaaaaaa aaaaaaaaaa aaaaaaaacg
     3961 cgtcgagggg aattaattct tgaagacgaa agggccaggt ggcacttttc ggggaaatgt
     4021 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4081 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     4141 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     4201 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     4261 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     4321 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     4381 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     4441 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     4501 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     4561 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     4621 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     4681 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     4741 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     4801 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     4861 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     4921 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     4981 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     5041 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5101 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5161 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5221 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5281 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     5341 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5401 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5461 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5521 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5581 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5641 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5701 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5761 gagctctaat acgactcact atagatgggc ggcgcatgag agaagcccag accaattacc
     5821 tacccaaaat ggagaaagtt cacgttgaca tcgaggaaga cagcccattc ctcagagctt
     5881 tgcagcggag cttcccgcag tttgaggtag aagccaagca ggtcactgat aatgaccatg
     5941 ctaatgccag agcgttttcg catctggctt caaaactgat cgaaacggag gtggacccat
     6001 ccgacacgat ccttgacatt ggaagtgcgc ccgcccgcag aatgtattct aagcacaagt
     6061 atcattgtat ctgtccgatg agatgtgcgg aagatccgga cagattgtat aagtatgcaa
     6121 ctaagctgaa gaaaaactgt aaggaaataa ctgataagga attggacaag aaaatgaagg
     6181 agctggccgc cgtcatgagc gaccctgacc tggaaactga gactatgtgc ctccacgacg
     6241 acgagtcgtg tcgctacgaa gggcaagtcg ctgtttacca ggatgtatac gcggttgacg
     6301 gaccgacaag tctctatcac caagccaata agggagttag agtcgcctac tggataggct
     6361 ttgacaccac cccttttatg tttaagaact tggctggagc atatccatca tactctacca
     6421 actgggccga cgaaaccgtg ttaacggctc gtaacatagg cctatgcagc tctgacgtta
     6481 tggagcggtc acgtagaggg atgtccattc ttagaaagaa gtatttgaaa ccatccaaca
     6541 atgttctatt ctctgttggc tcgaccatct accacgagaa gagggactta ctgaggagct
     6601 ggcacctgcc gtctgtattt cacttacgtg gcaagcaaaa ttacacatgt cggtgtgaga
     6661 ctatagttag ttgcgacggg tacgtcgtta aaagaatagc tatcagtcca ggcctgtatg
     6721 ggaagccttc aggctatgct gctacgatgc accgcgaggg attcttgtgc tgcaaagtga
     6781 cagacacatt gaacggggag agggtctctt ttcccgtgtg cacgtatgtg ccagctacat
     6841 tgtgtgacca aatgactggc atactggcaa cagatgtcag tgcggacgac gcgcaaaaac
     6901 tgctggttgg gctcaaccag cgtatagtcg tcaacggtcg cacccagaga aacaccaata
     6961 ccatgaaaaa ttaccttttg cccgtagtgg cccaggcatt tgctaggtgg gcaaaggaat
     7021 ataaggaaga tcaagaagat gaaaggccac taggactacg agatagacag ttagtcatgg
     7081 ggtgttgttg ggcttttaga aggcacaaga taacatctat ttataagcgc ccggataccc
     7141 aaaccatcat caaagtgaac agcgatttcc actcattcgt gctgcccagg ataggcagta
     7201 acacattgga gatcgggctg agaacaagaa tcaggaaaat gttagaggag cacaaggagc
     7261 cgtcacctct cattaccgcc gaggacgtac aagaagctaa gtgcgcagcc gatgaggcta
     7321 aggaggtgcg tgaagccgag gagttgcgcg cagctctacc acctttggca gctgatgttg
     7381 aggagcccac tctggaggca gacgtcgact tgatgttaca agaggctggg gccggctcag
     7441 tggagacacc tcgtggcttg ataaaggtta ccagctacga tggcgaggac aagatcggct
     7501 cttacgctgt gctttctccg caggctgtac tcaagagtga aaaattatct tgcatccacc
     7561 ctctcgctga acaagtcata gtgataacac actctggccg aaaagggcgt tatgccgtgg
     7621 aaccatacca tggtaaagta gtggtgccag agggacatgc aatacccgtc caggactttc
     7681 aagctctgag tgaaagtgcc accattgtgt acaacgaacg tgagttcgta aacaggtacc
     7741 tgcaccatat tgccacacat ggaggagcgc tgaacactga tgaagaatat tacaaaactg
     7801 tcaagcccag cgagcacgac ggcgaatacc tgtacgacat cgacaggaaa cagtgcgtca
     7861 agaaagaact agtcactggg ctagggctca caggcgagct ggtggatcct cccttccatg
     7921 aattcgccta cgagagtctg agaacacgac cagccgctcc ttaccaagta ccaaccatag
     7981 gggtgtatgg cgtgccagga tcaggcaagt ctggcatcat taaaagcgca gtcaccaaaa
     8041 aagatctagt ggtgagcgcc aagaaagaaa actgtgcaga aattataagg gacgtcaaga
     8101 aaatgaaagg gctggacgtc aatgccagaa ctgtggactc agtgctcttg aatggatgca
     8161 aacaccccgt agagaccctg tatattgacg aagcttttgc ttgtcatgca ggtactctca
     8221 gagcgctcat agccattata agacctaaaa aggcagtgct ctgcggggat cccaaacagt
     8281 gcggtttttt taacatgatg tgcctgaaag tgcattttaa ccacgagatt tgcacacaag
     8341 tcttccacaa aagcatctct cgccgttgca ctaaatctgt gacttcggtc gtctcaacct
     8401 tgttttacga caaaaaaatg agaacgacga atccgaaaga gactaagatt gtgattgaca
     8461 ctaccggcag taccaaacct aagcaggacg atctcattct cacttgtttc agagggtggg
     8521 tgaagcagtt gcaaatagat tacaaaggca acgaaataat gacggcagct gcctctcaag
     8581 ggctgacccg taaaggtgtg tatgccgttc ggtacaaggt gaatgaaaat cctctgtacg
     8641 cacccacctc agaacatgtg aacgtcctac tgacccgcac ggaggaccgc atcgtgtgga
     8701 aaacactagc cggcgaccca tggataaaaa cactgactgc caagtaccct gggaatttca
     8761 ctgccacgat agaggagtgg caagcagagc atgatgccat catgaggcac atcttggaga
     8821 gaccggaccc taccgacgtc ttccagaata aggcaaacgt gtgttgggcc aaggctttag
     8881 tgccggtgct gaagaccgct ggcatagaca tgaccactga acaatggaac actgtggatt
     8941 attttgaaac ggacaaagct cactcagcag agatagtatt gaaccaacta tgcgtgaggt
     9001 tctttggact cgatctggac tccggtctat tttctgcacc cactgttccg ttatccatta
     9061 ggaataatca ctgggataac tccccgtcgc ctaacatgta cgggctgaat aaagaagtgg
     9121 tccgtcagct ctctcgcagg tacccacaac tgcctcgggc agttgccact ggaagagtct
     9181 atgacatgaa cactggtaca ctgcgcaatt atgatccgcg cataaaccta gtacctgtaa
     9241 acagaagact gcctcatgct ttagtcctcc accataatga acacccacag agtgactttt
     9301 cttcattcgt cagcaaattg aagggcagaa ctgtcctggt ggtcggggaa aagttgtccg
     9361 tcccaggcaa aatggttgac tggttgtcag accggcctga ggctaccttc agagctcggc
     9421 tggatttagg catcccaggt gatgtgccca aatatgacat aatatttgtt aatgtgagga
     9481 ccccatataa ataccatcac tatcagcagt gtgaagacca tgccattaag cttagcatgt
     9541 tgaccaagaa agcttgtctg catctgaatc ccggcggaac ctgtgtcagc ataggttatg
     9601 gttacgctga cagggccagc gaaagcatca ttggtgctat agcgcggcag ttcaagtttt
     9661 cccgggtatg caaaccgaaa tcctcacttg aagagacgga agttctgttt gtattcattg
     9721 ggtacgatcg caaggcccgt acgcacaatt cttacaagct ttcatcaacc ttgaccaaca
     9781 tttatacagg ttccagactc cacgaagccg gatgtgcacc ctcatatcat gtggtgcgag
     9841 gggatattgc cacggccacc gaaggagtga ttataaatgc tgctaacagc aaaggacaac
     9901 ctggcggagg ggtgtgcgga gcgctgtata agaaattccc ggaaagcttc gatttacagc
     9961 cgatcgaagt aggaaaagcg cgactggtca aaggtgcagc taaacatatc attcatgccg
    10021 taggaccaaa cttcaacaaa gtttcggagg ttgaaggtga caaacagttg gcagaggctt
    10081 atgagtccat cgctaagatt gtcaacgata acaattacaa gtcagtagcg attccactgt
    10141 tgtccaccgg catcttttcc gggaacaaag atcgactaac ccaatcattg aaccatttgc
    10201 tgacagcttt agacaccact gatgcagatg tagccatata ctgcagggac aagaaatggg
    10261 aaatgactct caaggaagca gtggctagga gagaagcagt ggaggagata tgcatatccg
    10321 acgactcttc agtgacagaa cctgatgcag agctggtgag ggtgcatccg aagagttctt
    10381 tggctggaag gaagggctac agcacaagcg atggcaaaac tttctcatat ttggaaggga
    10441 ccaagtttca ccaggcggcc aaggatatag cagaaattaa tgccatgtgg cccgttgcaa
    10501 cggaggccaa tgagcaggta tgcatgtata tcctcggaga aagcatgagc agtattaggt
    10561 cgaaatgccc cgtcgaagag tcggaagcct ccacaccacc tagcacgctg ccttgcttgt
    10621 gcatccatgc catgactcca gaaagagtac agcgcctaaa agcctcacgt ccagaacaaa
    10681 ttactgtgtg ctcatccttt ccattgccga agtatagaat cactggtgtg cagaagatcc
    10741 aatgctccca gcctatattg ttctcaccga aagtgcctgc gtatattcat ccaaggaagt
    10801 atctcgtgga aacaccaccg gtagacgaga ctccggagcc atcggcagag aaccaatcca
    10861 cagaggggac acctgaacaa ccaccactta taaccgagga tgagaccagg actagaacgc
    10921 ctgagccgat catcatcgaa gaggaagaag aggatagcat aagtttgctg tcagatggcc
    10981 cgacccacca ggtgctgcaa gtcgaggcag acattcacgg gccgccctct gtatctagct
    11041 catcctggtc cattcctcat gcatccgact ttgatgtgga cagtttatcc atacttgaca
    11101 ccctggaggg agctagcgtg accagcgggg caacgtcagc cgagactaac tcttacttcg
    11161 caaagagtat ggagtttctg gcgcgaccgg tgcctgcgcc tcgaacagta ttcaggaacc
    11221 ctccacatcc cgctccgcgc acaagaacac cgtcacttgc acccagcagg gcctgctcga
    11281 gaaccagcct agtttccacc ccgccaggcg tgaatagggt gatcactaga gaggagctcg
    11341 aggcgcttac cccgtcacgc actcctagca ggtcggtctc gagaaccagc ctggtctcca
    11401 acccgccagg cgtaaatagg gtgattacaa gagaggagtt tgaggcgttc gtagcacaac
    11461 aacaatgacg gtttgatgcg ggtgcataca tcttttcctc cgacaccggt caagggcatt
    11521 t
//
