LOCUS       sequence-419583-        9869 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing mCherry fused with CXCR4 cDNA at the 
            C-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9869)
  AUTHORS   Aoki K, Hyuga M, Tarumoto Y, Nishibuchi G, Ueda A, Ochi Y, Sugino S,
            Mikami T, Kobushi H, Kato I, Akahane K, Inukai T, Takaori-Kondo A, 
            Takita J, Ogawa S, Yusa K
  TITLE     Canonical BAF complex regulates the oncogenic program in human 
            T-cell acute lymphoblastic leukemia.
  JOURNAL   Blood. 2023 Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857.
  PUBMED    37922452
REFERENCE   2  (bases 1 to 9869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2023
            Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857."
COMMENT     Sequence Label: pKLV-EF1a-mCherryCXCR4-W
FEATURES             Location/Qualifiers
     source          1..9869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     3..22
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3..21
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        92..395
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        397..595
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     550..570
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             613..793
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    840..965
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1462..1695
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1880..1924
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2222..2339
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2810..3991
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3041..3983
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3940..3960
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4011..4718
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNSAIIKEFLRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4156..4174)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4424..4443
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4737..4790
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    5514..5525
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     complement(5921..5941)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6339..6350)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6802..6819)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6808..7032
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7058..7078)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7058..7076)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7097..7113)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7097..7113)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7110..7132)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7121..7137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7145..7151,7152..7169,7170..7175))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7190..7211
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7328..7345)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7499..8087)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7579..7598)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8258..9049,9050..9118))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8881..8900
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9119..9223)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9249..9704)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9386..9407)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9598..9617
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9831..9853
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9845..9862
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9846..9862
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtaatacga ctcactatag ggcgaattga ctagttatta atagtaatca attacggggt
       61 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      121 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      181 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      241 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      301 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      361 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      421 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      481 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      541 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt
      601 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
      661 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
      721 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
      781 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
      841 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
      901 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
      961 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     1021 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     1081 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     1141 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     1201 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     1261 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt
     1321 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     1381 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     1441 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     1501 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     1561 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     1621 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     1681 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     1741 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     1801 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     1861 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     1921 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     1981 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     2041 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     2101 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     2161 agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca
     2221 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     2281 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     2341 gggtttatta cagggacagc agagatccag tttggttagt accgggccct acgcgtgagg
     2401 ggcgcggaag ccggggcgga ggaggagcca accccgggct gggctgagac ccgcagagga
     2461 agacgctcta gggatttgtc ccggactagc gagatggcaa ggctgaggac gggaggctga
     2521 ttgagaggcg aaggtacacc ctaatctcaa tacaaccttt ggagctaagc cagcaatggt
     2581 agagggaaga ttctgcacgt cccttccagg cggcctcccc gtcaccaccc cccccaaccc
     2641 gccccgaccg gagctgagag taattcatac aaaaggactc gcccctgcct tggggaatcc
     2701 cagggaccgt cgttaaactc ccactaacgt agaacccaga gatcgctgcg ttcccgcccc
     2761 ctcacccgcc cgctctcgtc atcactgagg tggagaagag catgcgtgag gctccggtgc
     2821 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     2881 caattgaacc ggtgcctaga gaaagtggcg cggggtaaac tgggaaagtg atgtcgtgta
     2941 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     3001 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc
     3061 ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacgcc
     3121 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt
     3181 cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
     3241 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
     3301 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
     3361 gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
     3421 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
     3481 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
     3541 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
     3601 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
     3661 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
     3721 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     3781 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     3841 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     3901 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     3961 aaagtttttt tcttccattt caggtgtcgt ggaagcttgg cgcgcccacc atggtgagca
     4021 agggcgagga ggataactcc gccatcatca aggagttcct gcgcttcaag gtgcacatgg
     4081 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     4141 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     4201 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     4261 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     4321 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc tctgcaggac ggcgagttca
     4381 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     4441 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     4501 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     4561 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     4621 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     4681 gccgccactc caccggcggc atggacgagc tgtataaggg cggcgggtcc ggaggagagg
     4741 gcagaggatc tcttctaaca tgcggtgacg tggaggagaa tcctggccca atggagggga
     4801 tcagtatata cacttcagat aactacaccg aggaaatggg ctcaggggac tatgactcca
     4861 tgaaggaacc ctgtttccgt gaagaaaatg ctaatttcaa taaaatcttc ctgcccacca
     4921 tctactccat catcttctta actggcattg tgggcaatgg attggtcatt ctggtcatgg
     4981 gttaccagaa gaaactgaga agcatgacgg acaagtacag gctgcacctg tcagtggccg
     5041 acctcctctt tgtcatcacg cttcccttct gggcagttga tgccgtggca aactggtact
     5101 ttgggaactt cctatgcaag gcagtccatg tcatctacac agtcaacctc tacagcagtg
     5161 tcctcatcct ggccttcatc agtctggacc gctacctggc catcgtccac gccaccaaca
     5221 gtcagagacc aaggaagctg ttggctgaaa aggtggtcta tgttggcgtc tggatccctg
     5281 ccctgctgct gactattccc gacttcatct ttgccaacgt cagtgaggca gatgacagat
     5341 atatctgtga ccgcttctac cccaatgact tgtgggtggt tgtgttccag tttcagcaca
     5401 tcatggttgg ccttatcctg cctggtattg tcatcctgtc ctgctattgc attatcatct
     5461 ccaagctgtc acactccaag ggccaccaga agcgcaaggc cctcaagacc acagtcatcc
     5521 tcatcctggc tttcttcgcc tgttggctgc cttactacat tgggatcagc atcgactcct
     5581 tcatcctcct ggaaatcatc aagcaagggt gtgagtttga gaacactgtg cacaagtgga
     5641 tttccatcac cgaggcccta gctttcttcc actgttgtct gaaccccatc ctctatgctt
     5701 tccttggagc caaatttaaa acctctgccc agcacgcact cacctctgtg agcagagggt
     5761 ccagcctcaa gatcctctcc aaaggaaagc gaggtggaca ttcatctgtt tccactgagt
     5821 ctgagtcttc aagttttcac tccagctaaa agtaagaatt cgcggccgct caacctctgg
     5881 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5941 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     6001 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     6061 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     6121 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     6181 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6241 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     6301 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6361 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     6421 agacgagtcg gatctccctt tgggccgcct ccccgcaggt acctttaaga ccaatgactt
     6481 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     6541 ttcactccca aagaagtcaa gatctgcttt ttgcctgtac tgggtctctc tggttagacc
     6601 agagtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6661 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6721 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6781 cagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     6841 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     6901 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     6961 tggggcagga cagcaagggg gaggattggg aagtcaatag caggcatgct ggggatgcgg
     7021 tgggctctat gggcggccgt taattaagct tttgttccct ttagtgaggg ttaattgcgc
     7081 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     7141 cacacaacat acgagccggg agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     7201 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7261 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     7321 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     7381 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     7441 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     7501 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     7561 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     7621 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     7681 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     7741 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     7801 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     7861 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     7921 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     7981 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8041 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8101 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8161 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8221 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8281 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     8341 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     8401 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     8461 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     8521 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     8581 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     8641 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     8701 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     8761 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     8821 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     8881 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     8941 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9001 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9061 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9121 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9181 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     9241 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     9301 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     9361 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     9421 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     9481 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     9541 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     9601 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     9661 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     9721 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     9781 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     9841 acgttgtaaa acgacggcca gtgagcgcg
//
