LOCUS       sequence-419589-       11489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing mCherry fused with BCL11B cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11489)
  AUTHORS   Aoki K, Hyuga M, Tarumoto Y, Nishibuchi G, Ueda A, Ochi Y, Sugino S,
            Mikami T, Kobushi H, Kato I, Akahane K, Inukai T, Takaori-Kondo A, 
            Takita J, Ogawa S, Yusa K
  TITLE     Canonical BAF complex regulates the oncogenic program in human 
            T-cell acute lymphoblastic leukemia.
  JOURNAL   Blood. 2023 Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857.
  PUBMED    37922452
REFERENCE   2  (bases 1 to 11489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2023
            Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857."
COMMENT     Sequence Label: pKLV-EF1a-mCherryBCL11B-W
FEATURES             Location/Qualifiers
     source          1..11489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        116..419
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        421..619
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     574..594
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             637..817
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    864..989
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1486..1719
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1904..1948
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2246..2363
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2834..4015
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3065..4007
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3964..3984
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4035..4742
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNSAIIKEFLRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4180..4198)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4448..4467
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4761..4814
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(7565..7585)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7983..7994)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8446..8463)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8452..8676
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8702..8722)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8702..8720)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8741..8757)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8741..8757)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8754..8776)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8765..8781
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8789..8795,8796..8813,8814..8819))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8834..8855
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8972..8989)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9143..9731)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9223..9242)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9902..10693,10694..10762))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10525..10544
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10763..10867)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10893..11348)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11030..11051)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11242..11261
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(11475..11489,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(11489,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgactagtt
       61 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      121 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      181 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      241 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      301 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      361 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      421 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      481 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      541 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      601 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      661 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      721 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      781 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      841 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      901 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      961 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     1021 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     1081 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     1141 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1201 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1261 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1321 agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     1381 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     1441 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     1501 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     1561 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     1621 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     1681 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     1741 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     1801 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     1861 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     1921 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     1981 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     2041 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     2101 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     2161 agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca
     2221 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     2281 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     2341 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt
     2401 tagtaccggg ccctacgcgt gaggggcgcg gaagccgggg cggaggagga gccaaccccg
     2461 ggctgggctg agacccgcag aggaagacgc tctagggatt tgtcccggac tagcgagatg
     2521 gcaaggctga ggacgggagg ctgattgaga ggcgaaggta caccctaatc tcaatacaac
     2581 ctttggagct aagccagcaa tggtagaggg aagattctgc acgtcccttc caggcggcct
     2641 ccccgtcacc acccccccca acccgccccg accggagctg agagtaattc atacaaaagg
     2701 actcgcccct gccttgggga atcccaggga ccgtcgttaa actcccacta acgtagaacc
     2761 cagagatcgc tgcgttcccg ccccctcacc cgcccgctct cgtcatcact gaggtggaga
     2821 agagcatgcg tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     2881 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaagt ggcgcggggt
     2941 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     3001 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     3061 acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg
     3121 cgtgccttga attacttcca cgcccctggc tgcagtacgt gattcttgat cccgagcttc
     3181 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
     3241 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     3301 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     3361 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     3421 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     3481 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     3541 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     3601 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     3661 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     3721 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     3781 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     3841 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     3901 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     3961 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtggaagc
     4021 ttggcgcgcc caccatggtg agcaagggcg aggaggataa ctccgccatc atcaaggagt
     4081 tcctgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     4141 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     4201 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     4261 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     4321 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     4381 cctctctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     4441 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     4501 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     4561 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     4621 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     4681 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtata
     4741 agggcggcgg gtccggagga gagggcagag gatctcttct aacatgcggt gacgtggagg
     4801 agaatcctgg cccaatgtcc cgccgcaaac agggcaaccc gcagcacttg tcccagaggg
     4861 agctcatcac cccagaggct gaccatgtgg aggccgccat cctcgaagaa gacgagggtc
     4921 tggagataga ggagccaagt ggcctggggc tgatggtggg tggccccgac cctgacctgc
     4981 tcacctgtgg ccagtgtcaa atgaacttcc ccttggggga catcctggtt tttatagagc
     5041 acaaaaggaa gcagtgtggc ggcagcttgg gtgcctgcta tgacaaggcc ctggacaagg
     5101 acagcccgcc accctcctca cgctccgagc tcaggaaagt gtccgagccg gtggagatcg
     5161 ggatccaagt cacccccgac gaagatgacc acctgctctc acccacgaaa ggcatctgtc
     5221 ccaagcagga gaacattgca gggccgtgca ggcctgccca gctgccagcg gtggccccca
     5281 tagctgcctc ctcccaccct cactcatccg tgatcacttc acctctgcgt gccctgggcg
     5341 ctctcccgcc ctgcctcccc ctgccgtgct gcagcgcgcg cccggtctcg ggtgacggga
     5401 ctcagggtga gggtcagacg gaggctccct ttggatgcca gtgtcagttg tcaggtaaag
     5461 atgagccttc cagctacatt tgcacaacat gcaagcagcc cttcaacagc gcgtggttcc
     5521 tgctgcagca cgcgcagaac acgcacggct tccgcatcta cctggagccc gggccggcca
     5581 gcagctcgct cacgccgcgg ctcaccatcc cgccgccgct cgggccggag gccgtggcgc
     5641 agtccccgct catgaatttc ctgggcgaca gcaacccctt caacctgctg cgcatgacgg
     5701 gccccatcct gcgggaccac ccgggcttcg gcgagggccg cctgccgggc acgccgcctc
     5761 tcttcagtcc cccgccgcgc caccacctgg acccgcaccg cctcagtgcc gaggagatgg
     5821 ggctcgtcgc ccagcacccc agtgccttcg accgagtcat gcgcctgaac cccatggcca
     5881 tcgactcgcc cgccatggac ttctcgcggc ggctccgcga gctggcgggc aacagctcca
     5941 cgccgccgcc cgtgtccccg ggccgcggca accctatgca ccggctcctg aaccccttcc
     6001 agcccagccc caagtccccg ttcctgagca cgccgccgct gccgcccatg ccccctggcg
     6061 gcacgccgcc cccgcagccg ccagccaaga gcaagtcgtg cgagttctgc ggcaagacct
     6121 tcaagttcca gagcaatctc atcgtgcacc ggcgcagtca cacgggcgag aagccctaca
     6181 agtgccagct gtgcgaccac gcgtgctcgc aggccagcaa gctcaagcgc cacatgaaga
     6241 cgcacatgca caaggccggc tcgctggccg gccgctccga cgacgggctc tcggccgcca
     6301 gctcccccga gcccggcacc agcgagctgg cgggcgaggg cctcaaggcg gccgacggtg
     6361 acttccgcca ccacgagagc gacccgtcgc tgggccacga gccggaggag gaggacgagg
     6421 aggaggagga ggaggaggag gagctgctac tggagaacga gagccggccc gagtcgagct
     6481 tcagcatgga ctcggagctg agccgcaacc gcgagaacgg cggtggtggg gtgcccgggg
     6541 tcccgggcgc ggggggcggc gcggccaagg cgctggctga cgagaaggcg ctggtgctgg
     6601 gcaaggtcat ggagaacgtg ggcctaggcg cactgccgca gtacggcgag ctcctggccg
     6661 acaagcagaa gcgcggcgcc ttcctgaagc gtgcggcggg cggcggggac gcgggcgacg
     6721 acgacgacgc gggcggctgc ggggacgcgg gcgcgggcgg cgcggtcaac gggcgcgggg
     6781 gcggcttcgc gccaggcacc gagcccttcc ccgggctctt cccgcgcaag cccgcgccgc
     6841 tgcccagccc cgggctcaac agcgccgcca agcgcatcaa ggtggagaag gacctggagc
     6901 tgccgcccgc cgcgctcatc ccgtccgaga acgtgtactc gcagtggctg gtgggctacg
     6961 cggcgtcgcg gcacttcatg aaggacccct tcctgggctt cacggacgca cgacagtcgc
     7021 ccttcgccac gtcgtccgag cactcgtccg agaacggcag cctgcgcttc tccacgccgc
     7081 ccggggacct gctggacggc ggcctctcgg gccgcagcgg cacggccagc ggaggcagca
     7141 ccccgcacct gggcggcccg ggccccgggc ggcccagctc caaggagggc cgccgcagcg
     7201 acacgtgcga gtactgcggc aaggtgttca agaactgcag caacttgacg gtgcaccggc
     7261 ggagccacac cggcgagcgg ccttacaagt gcgagctgtg caactacgcg tgcgcgcaga
     7321 gcagcaagct cacgcgccac atgaagacgc acgggcagat cggcaaggag gtgtaccgct
     7381 gcgacatctg ccagatgccc ttcagcgtct acagcaccct ggagaaacac atgaaaaagt
     7441 ggcacggcga gcacttgctg actaacgacg tcaaaatcga gcaggccgag aggagctaag
     7501 aattcgcggc cgctcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     7561 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     7621 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     7681 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     7741 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7801 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7861 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     7921 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7981 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     8041 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     8101 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     8161 aaaagggggg actggaaggg ctaattcact cccaaagaag tcaagatctg ctttttgcct
     8221 gtactgggtc tctctggtta gaccagagtc tctctggtta gaccagatct gagcctggga
     8281 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     8341 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     8401 ttagtcagtg tggaaaatct ctagcagttt aaacccgctg atcagcctcg actgtgcctt
     8461 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     8521 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     8581 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagtca
     8641 atagcaggca tgctggggat gcggtgggct ctatgggcgg ccgttaatta agcttttgtt
     8701 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     8761 gaaattgtta tccgctcaca attccacaca acatacgagc cgggagcata aagtgtaaag
     8821 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     8881 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     8941 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     9001 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     9061 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     9121 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     9181 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     9241 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     9301 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     9361 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     9421 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     9481 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     9541 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     9601 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9661 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9721 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9781 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9841 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9901 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     9961 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    10021 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    10081 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    10141 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    10201 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    10261 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    10321 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    10381 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    10441 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    10501 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    10561 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    10621 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    10681 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    10741 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    10801 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10861 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
    10921 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
    10981 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
    11041 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
    11101 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
    11161 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
    11221 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
    11281 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
    11341 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
    11401 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
    11461 aacgccaggg ttttcccagt cacgacgtt
//
