LOCUS       sequence-419600-       10237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NIS-Seq-compatible lentiviral vector for expressing sgRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10237)
  AUTHORS   Fandrey CI, Jentzsch M, Konopka P, Hoch A, Blumenstock K, Zackria A,
            Maasewerd S, Lovotti M, Lapp DJ, Gohr FN, Suwara P, Swiezewski J, 
            Rossnagel L, Gobs F, Cristodaro M, Muhandes L, Behrendt R, Lam MC, 
            Walgenbach KJ, Bald T, Schmidt FI, Latz E, Schmid-Burgk JL
  TITLE     NIS-Seq enables cell-type-agnostic optical perturbation screening.
  JOURNAL   Nat Biotechnol. 2024 Dec 19. doi: 10.1038/s41587-024-02516-5.
  PUBMED    39702735
REFERENCE   2  (bases 1 to 10237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Dec 19. doi: 10.1038/s41587-024-02516-5."
COMMENT     Sequence Label: CROPseq-iT7
FEATURES             Location/Qualifiers
     source          1..10237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     240..260
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        288..514
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             515..695
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    742..867
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1360..1593
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1778..1822
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2003..2120
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2259..3437
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2490..3428
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3385..3405
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3450..4049
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3450..3469)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3946..3966
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4065..4653
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4118..4138)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4536..4547)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4809..5057
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4809..4829
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4980..4999
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        6939..7014
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(7025..7043)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     LTR             7090..7270
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7348..7469
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7385..7404)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7439..7458
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7509..7644
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7665..7683)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7693..7710)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7693..7709)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7702..7724)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7851..8306
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7938..7957)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8148..8169
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8332..8436
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8437..8505,8506..9297)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8655..8674)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9468..10056
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9957..9976
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10210..10227
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
      301 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
      361 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
      421 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
      481 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
      541 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      601 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      661 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      721 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      781 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      841 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      901 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      961 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     1021 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1081 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1141 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1201 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1261 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1321 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1381 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1441 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1501 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1561 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1621 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1681 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1741 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1801 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1861 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1921 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga atcttgagac
     1981 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2041 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2101 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
     2161 ggctcgaggg ggcccgggtg caaagatgga taaagtttta aacagagagg aatctttgca
     2221 gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg
     2281 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg
     2341 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2401 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2461 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2521 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2581 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2641 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2701 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2761 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2821 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2881 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2941 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     3001 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     3061 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     3121 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     3181 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     3241 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3301 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3361 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3421 catttcaggt gtcgtgacgt acggccacca tgaccgagta caagcccacg gtgcgcctcg
     3481 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     3541 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     3601 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     3661 ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     3721 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     3781 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggagtct
     3841 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     3901 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     3961 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     4021 ggtgcatgac ccgcaagccc ggtgcctgaa cgcgttaagt cgacaatcaa cctctggatt
     4081 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     4141 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     4201 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     4261 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     4321 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     4381 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     4441 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     4501 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     4561 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     4621 cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa
     4681 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     4741 ctcccaacga agacaagatt tcaccattat cgtttcagac ccacctccca accccgaggg
     4801 gacccagaga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
     4861 ttagagagat aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt
     4921 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
     4981 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     5041 ggaaaggacg aaacaccgga gacggttgta aatgagcaca caaaatacac atgctaaaat
     5101 attatattct atgaccttta taaaatcaac caaaatcttc tttttaataa ctttagtatc
     5161 aataattaga atttttatgt tcctttttgc aaacttttaa taaaaatgag caaaataaaa
     5221 aaacgctagt tttagtaact cgcgttgttt tcttcacctt taataatagc tactccacca
     5281 cttgttccta agcggtcagc tcctgcttca atcatttttt gagcatcttc aaatgttcta
     5341 actccaccag ctgctttaac taaagcattg tctttaacaa ctgacttcat tagtttaaca
     5401 tcttcaaatg ttgcacctga ttttgaaaat cctgttgatg ttttaacaaa ttctaatcca
     5461 gcttcaacag ctatttcaca agctttcatg atttcttctt ttgttaataa acaattttcc
     5521 ataatacatt taacaacatg tgatccagct gcttttttta cagctttcat gtcttctaaa
     5581 actaattcat aatttttgtc ttttaatgca ccaatattta ataccatatc aatttctgtt
     5641 gcaccatctt taattgcttc agaaacttcg aatgcttttg tagctgttgt gcatgcacct
     5701 agaggaaaac ctacaacatt tgttattcct acatttgtgc cttttaataa ttctttacaa
     5761 tagcttgttc aatatgaatt aacacaaact gttgcaaaat caaattcaat tgcttcatca
     5821 cataattgtt taatttcagc tttcgtagca tcttgtttta ataatgtgtg atctatatat
     5881 ttgtttagtt tcattttttc tcctatatat tcatttttaa ttttaattct ttaataattt
     5941 cgtctacttt aactttagcg ttttgaacag attcaccaac acctataaaa taaattttta
     6001 gtttaggttc agttccactt gggcgaacag caaatcatga cttatcttct aaataaaatt
     6061 ttagtaagtc ttgtcctggc atattataca ttccatcgat gtagtcttca acattaacaa
     6121 ctttaagtcc agcaatttga gttaagggtg ttgctctcaa tgatttcatt aatggttcaa
     6181 tttttaattt cttttcttct ggtttaaaat tcaagtttaa agtgaaagtg taatatgcac
     6241 ccatttcttt aaataaatct tctaaatagt ctactaatgt tttattttgt tttttataaa
     6301 atcaagcagc ctctgctatt aatatagaag cttgtattcc atctttatct ctagctgagt
     6361 catcaattac atatccataa ctttcttcat aagcaaaaac aaaatttaat ccgttatctt
     6421 cttctttagc aatttctcta cccattcatt taaatccagt taaagttttt acaatattaa
     6481 ctccatattt ttcatgagcg attctatcac ccaaatcact tgttacaaaa cttgaatata
     6541 gagccggatt ttttggaatg ctatttaagc gttttagatt tgataatttt caatcaatta
     6601 aaattggtcc tgtttgattt ccatctaatc ttacaaaatg accatcatgt tttattgcca
     6661 ttccaaatct gtcagcatct gggtcattca taataataat atctgcatca tgtttaatac
     6721 catattcaag cggtattttt catgcaggat caaattctgg atttggattt acaacatttt
     6781 taaatgtttc atcttcaaat gcatgctctt caacctcaat aacgttatat cctgattcac
     6841 gtaatatttt tggggtaaat ttagttcctg ttccattaac tgcgctaaaa ataattttta
     6901 aatctttttt agcttcttgc tcttttttgt acgtctctgt tttagagcta gaaatagcaa
     6961 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
     7021 gatccctata gtgagtcgta ttaaagcttg gcgtaactag atcttgagac actgcttttt
     7081 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7141 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7201 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7261 atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca
     7321 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     7381 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7441 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     7501 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7561 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     7621 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     7681 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     7741 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     7801 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     7861 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     7921 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     7981 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     8041 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     8101 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     8161 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     8221 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     8281 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     8341 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     8401 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     8461 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     8521 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     8581 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     8641 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     8701 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     8761 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     8821 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     8881 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     8941 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     9001 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     9061 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     9121 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     9181 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     9241 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     9301 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     9361 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     9421 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     9481 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     9541 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     9601 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     9661 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     9721 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     9781 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     9841 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     9901 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     9961 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    10021 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    10081 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    10141 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    10201 accgagcgca gcgagtcagt gagcgaggaa gcggaag
//
