LOCUS       sequence-419601-        8342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A SCHEMA evolved AAV variant effective at targeting subventricular 
            NSCs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Ojala DS, Sun S, Santiago-Ortiz JL, Shapiro MG, Romero PA, Schaffer 
            DV
  TITLE     In Vivo Selection of a Computationally Designed SCHEMA AAV Library 
            Yields a Novel Variant for Infection of Adult Neural Stem Cells in 
            the SVZ.
  JOURNAL   Mol Ther. 2018 Jan 3;26(1):304-319. doi: 
            10.1016/j.ymthe.2017.09.006. Epub 2017 Sep 8.
  PUBMED    28988711
REFERENCE   2  (bases 1 to 8342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymthe.2017.09.006"; journalName: "Mol Ther"; date: 
            "2018-01-3- 3"; volume: "26"; issue: "1"; pages: "304-319"
COMMENT     Sequence Label: pAAV SCH9
FEATURES             Location/Qualifiers
     source          1..8342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     repeat_region   243..345
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     CDS             4421..4432
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     rep_origin      5358..5871
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5445..5464)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5655..5676
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6353..6372)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6472..6494
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6532..6550)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6618..6722
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6723..6791,6792..7583)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6941..6960)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7754..8342
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8243..8262
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagaatt
      241 cccatcatca ataatatacc ttattttgga ttgaagccaa tatgataatg agggggtgga
      301 gtttgtgacg tggcgcgggg cgtgggaacg gggcgggtga cgtagtagct ctagaggtcc
      361 tgtattagag gtcacgtgag tgttttgcga cattttgcga caccatgtgg tcacgctggg
      421 tatttaagcc cgagtgagca cgcagggtct ccattttgaa gcgggaggtt tgaacgcgca
      481 gccaccacgg cggggtttta cgagattgtg attaaggtcc ccagcgacct tgacgagcat
      541 ctgcccggca tttctgacag ctttgtgaac tgggtggccg agaaggaatg ggagttgccg
      601 ccagattctg acatggatct gaatctgatt gagcaggcac ccctgaccgt ggccgagaag
      661 ctgcagcgcg actttctgac ggaatggcgc cgtgtgagta aggccccgga ggcccttttc
      721 tttgtgcaat ttgagaaggg agagagctac ttccacatgc acgtgctcgt ggaaaccacc
      781 ggggtgaaat ccatggtttt gggacgtttc ctgagtcaga ttcgcgaaaa actgattcag
      841 agaatttacc gcgggatcga gccgactttg ccaaactggt tcgcggtcac aaagaccaga
      901 aatggcgccg gaggcgggaa caaggtggtg gatgagtgct acatccccaa ttacttgctc
      961 cccaaaaccc agcctgagct ccagtgggcg tggactaata tggaacagta tttaagcgcc
     1021 tgtttgaatc tcacggagcg taaacggttg gtggcgcagc atctgacgca cgtgtcgcag
     1081 acgcaggagc agaacaaaga gaatcagaat cccaattctg atgcgccggt gatcagatca
     1141 aaaacttcag ccaggtacat ggagctggtc gggtggctcg tggacaaggg gattacctcg
     1201 gagaagcagt ggatccagga ggaccaggcc tcatacatct ccttcaatgc ggcctccaac
     1261 tcgcggtccc aaatcaaggc tgccttggac aatgcgggaa agattatgag cctgactaaa
     1321 accgcccccg actacctggt gggccagcag cccgtggagg acatttccag caatcggatt
     1381 tataaaattt tggaactaaa cgggtacgat ccccaatatg cggcttccgt ctttctggga
     1441 tgggccacga aaaagttcgg caagaggaac accatctggc tgtttgggcc tgcaactacc
     1501 gggaagacca acatcgcgga ggccatagcc cacactgtgc ccttctacgg gtgcgtaaac
     1561 tggaccaatg agaactttcc cttcaacgac tgtgtcgaca agatggtgat ctggtgggag
     1621 gaggggaaga tgaccgccaa ggtcgtggag tcggccaaag ccattctcgg aggaagcaag
     1681 gtgcgcgtgg accagaaatg caagtcctcg gcccagatag acccgactcc cgtgatcgtc
     1741 acctccaaca ccaacatgtg cgccgtgatt gacgggaact caacgacctt cgaacaccag
     1801 cagccgttgc aagaccggat gttcaaattt gaactcaccc gccgtctgga tcatgacttt
     1861 gggaaggtca ccaagcagga agtcaaagac tttttccggt gggcaaagga tcacgtggtt
     1921 gaggtggagc atgaattcta cgtcaaaaag ggtggagcca agaaaagacc cgcccccagt
     1981 gacgcagata taagtgagcc caaacgggtg cgcgagtcag ttgcgcagcc atcgacgtca
     2041 gacgcggaag cttcgatcaa ctacgcagac aggtaccaaa acaaatgttc tcgtcacgtg
     2101 ggcatgaatc tgatgctgtt tccctgcaga caatgcgaga gaatgaatca gaattcaaat
     2161 atctgcttca ctcacggaca gaaagactgt ttagagtgct ttcccgtgtc agaatctcaa
     2221 cccgtttctg tcgtcaaaaa ggcgtatcag aaactgtgct acattcatca tatcatggga
     2281 aaggtgccag acgcttgcac tgcctgcgat ctggtcaatg tggatttgga tgactgcatc
     2341 tttgaacaat aaatgattta aatcaggtat ggctgccgat ggttatcttc cagattggct
     2401 cgaggacaac ctctctgagg gcattcgcga gtggtgggac ctgaaacctg gagccccgaa
     2461 acccaaagcc aaccagcaaa agcaggacga cggccggggt ctggtgcttc ctggctacaa
     2521 gtacctcgga cccttcaacg gactcgacaa gggggagccc gtcaacgcgg cggatgcagc
     2581 ggccctcgaa cacgacaagg cctacgacca gcagctcaaa gcgggtgaca atccgtacct
     2641 gcggtataac cacgccgacg ccgagtttca ggagcgtctg caagaagata cgtcttttgg
     2701 gggcaacctc gggcgagcag tcttccaggc caagaagagg gttctcgaac ctcttggtct
     2761 ggttgaggaa gcggctaaga cggctcctgg aaagaagagg cctgtagagc agtctcctca
     2821 ggaaccggac tcctccgcgg gtattggcaa atcgggtgca cagcccgcta aaaagagact
     2881 caatttcggt cagactggcg acacagagtc agtcccagac cctcaaccaa tcggagaacc
     2941 tcccgcagcc ccctcaggtg tgggatctct tacaatggct tcaggtggtg gcgcaccagt
     3001 ggcagacaat aacgaaggtg ccgatggagt gggtagttcc tcgggaaatt ggcattgcga
     3061 ttcccaatgg ctgggggaca gagtcatcac caccagcacc cgaacctggg ccctgcccac
     3121 ctacaacaat cacctctaca agcaaatctc caacagcaca tctggaggat cttcaaatga
     3181 caacgcctac ttcggctaca gcaccccctg ggggtatttt gactttaaca gattccactg
     3241 ccacttttca ccacgtgact ggcagcgact catcaacaac aactggggat tccggcccaa
     3301 gagactcagc ttcaagctct tcaacatcca ggtcaaggag gtcacgcaga atgaaggcac
     3361 caagaccatc gccaataacc tcaccagcac catccaggtc tttacggact cagactatca
     3421 gctcccgtac gtgctcgggt cggctcacga gggctgcctc ccgccgttcc cagcggacgt
     3481 tttcatgatt cctcagtacg ggtatctgac gcttaatgat ggaagccagg ccgtgggtcg
     3541 ttcgtccttt tactgcctgg aatatttccc gtcgcaaatg ctaagaacgg gtaacaactt
     3601 ccagttcagc tacgagtttg agaacgtacc tttccatagc agctacgctc acagccaaag
     3661 cctggaccga ctaatgaatc cactcatcga ccaatacttg tactatctct caaagactat
     3721 taacggttct ggacagaatc aacaaacgct aaaattcagt gtggccggac ccagcaacat
     3781 ggctgtccag ggaagaaact ggcttcctgg accctgttac cgccagcagc gagtatcaaa
     3841 gacatctgcg gataacaaca acagtgaata ctcgtggact ggagctacca agtaccacct
     3901 caatggcaga gactctctgg tgaatccggg cccggccatg gcaagccaca aggacgatga
     3961 agaaaagttt tttcctcaga gcggggttct catctttggg aagcaaggct cagagaaaac
     4021 aaatgtggac attgaaaagg tcatgattac agacgaagag gaaatcagga caaccaatcc
     4081 cgtggctacg gagcagtatg gttctgtatc taccaacctc cagagaggca acagacaagc
     4141 agctaccgca gatgtcaaca cacaaggcgt tcttccaggc atggtctggc aggacagaga
     4201 cgtgtacctg caaggaccca tttgggccaa aattcctcac acggacggca actttcaccc
     4261 ttctccgctg atgggagggt ttggaatgaa gcacccgcct cctcagatcc tcatcaaaaa
     4321 cacacctgta cctgcggatc ctccaacggc cttcaacaag gacaagctga actctttcat
     4381 cacccagtat tctactggcc aagtcagcgt ggagatcgag tgggagctgc agaaggaaaa
     4441 cagcaagcgc tggaacccgg agatccagta cacttccaac tattacaagt ctaataatgt
     4501 tgaatttgct gttaatactg aaggtgtata tagtgaaccc cgccccattg gcaccagata
     4561 cctgactcgt aatctgtaat tgcggccgct tgttaatcaa taaaccgttt aattcgtttc
     4621 agttgaactt tggtctctgc gtatttcttt cttatctagt ttccatgctc tagaggtcct
     4681 gtattagagg tcacgtgagt gttttgcgac attttgcgac accatgtggt cacgctgggt
     4741 atttaagccc gagtgagcac gcagggtctc cattttgaag cgggaggttt gaacgcgcag
     4801 ccaccacggc ggggttttac gagattgtga ttaaggtccc cagcgacctt gacgagcatc
     4861 tgcccggcat ttctgacagc tttgtgaact gggtggccga gaaggaatgg gagttgccgc
     4921 cagattctga catggatctg aatctgattg agcaggcacc cctgaccgtg gccgagaagc
     4981 tgcatcgctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     5041 gcctgaatgg cgaatggcga ttccgttgca atggctggcg gtaatattgt tctggatatt
     5101 accagcaagg ccgatagttt gagttcttct actcaggcaa gtgatgttat tactaatcaa
     5161 agaagtattg cgacaacggt taatttgcgt gatggacaga ctcttttact cggtggcctc
     5221 actgattata aaaacacttc tcaggattct ggcgtaccgt tcctgtctaa aatcccttta
     5281 atcggcctcc tgtttagctc ccgctctgat tctaacgagg aaagcacgtt atacgtgctc
     5341 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     5401 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     5461 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5521 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     5581 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5641 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     5701 ctattctttt gatttataag ggattttgcc gatttcggca tattggttaa aaaatgagct
     5761 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaaatatt
     5821 tgcttataca atcttcctgt ttttggggct tttctgatta tcaaccgggg tacatatgat
     5881 tgacatgcta gttttacgat taccgttcat cgattctctt gtttgctcca gactctcagg
     5941 caatgacctg atagcctttg tagagacctc tcaaaaatag ctaccctctc cggcatgaat
     6001 ttatcagcta gaacggttga atatcatatt gatggtgatt tgactgtctc cggcctttct
     6061 cacccgtttg aatctttacc tacacattac tcaggcattg catttaaaat atatgagggt
     6121 tctaaaaatt tttatccttg cgttgaaata aaggcttctc ccgcaaaagt attacagggt
     6181 cataatgttt ttggtacaac cgatttagct ttatgctctg aggctttatt gcttaatttt
     6241 gctaattctt tgccttgcct gtatgattta ttggatgttg gaatcgcctg atgcggtatt
     6301 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     6361 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     6421 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     6481 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     6541 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     6601 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6661 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6721 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6781 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6841 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     6901 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     6961 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7021 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7081 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     7141 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7201 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7261 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7321 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7381 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7441 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7501 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7561 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7621 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7681 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7741 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7801 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7861 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     7921 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7981 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8041 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8101 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8161 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8221 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8281 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8341 aa
//
