LOCUS       sequence-419604-       11739 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG-Cyclin D1, SS-Cyclin D2 and HA-Cyclin D3 in mammalian
            cells from an inducible lentiviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11739)
  AUTHORS   Rona G, Miwatani-Minter B, Zhang Q, Goldberg HV, Kerzhnerman MA, 
            Howard JB, Simoneschi D, Lane E, Hobbs JW, Sassani E, Wang AA, 
            Keegan S, Laverty DJ, Piett CG, Pongor LS, Xu ML, Andrade J, Thomas 
            A, Sicinski P, Askenazi M, Ueberheide B, Fenyo D, Nagel ZD, Pagano M
  TITLE     CDK-independent role of D-type cyclins in regulating DNA mismatch 
            repair.
  JOURNAL   Mol Cell. 2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010.
  PUBMED    38458201
REFERENCE   2  (bases 1 to 11739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010."
COMMENT     Sequence Label: FLAG-D1-IRES-SS-D2-IRES-HA-D3
FEATURES             Location/Qualifiers
     source          1..11739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1929..1949
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1955..1973
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1991..2009
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2027..2045
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2063..2081
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2099..2117
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2135..2153
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2171..2189
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2234..2258
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2325..2349
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2350..2359
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2371..2394
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      3017..3026
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(3305..3879,3880..3882,3883..3891)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             join(3904..3909,3910..3933,3934..3969,3970..3993)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     protein_bind    complement(4870..4894)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(4895..5469,5470..5472,5473..5481)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             5494..5520
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(6421..6441)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    6454..7042
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6507..6527)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6925..6936)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7073..7572
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7108..7131)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7505..7524
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7589..8188
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7589..7608)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8085..8105
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8260..8493
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8565..8686
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8602..8621)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8656..8675
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8726..8861
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8881..8900)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8882..8900)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8910..8927)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8910..8926)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8919..8941)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9068..9523
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9155..9174)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9365..9386
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9549..9653
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9654..9722,9723..10514)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9872..9891)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10685..11273
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11174..11193
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11427..11444
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11561..11582
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11597..11602,11603..11620,11621..11627)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11635..11651
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11640..11662
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11659..11675
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11659..11675
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11694..11714
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11696..11714
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtatca actttgtata gaaaagttgt ttactcccta tcagtgatag agaacgtatg
     1981 aagagtttac tccctatcag tgatagagaa cgtatgcaga ctttactccc tatcagtgat
     2041 agagaacgta taaggagttt actccctatc agtgatagag aacgtatgac cagtttactc
     2101 cctatcagtg atagagaacg tatctacagt ttactcccta tcagtgatag agaacgtata
     2161 tccagtttac tccctatcag tgatagagaa cgtataagct ttaggcgtgt acggtgggcg
     2221 cctataaaag cagagctcgt ttagtgaacc gtcagatcgc ctggagcaat tccacaacac
     2281 ttttgtctta taccaacttt ccgtaccact tcctaccctc gtaaacaagt ttgtacaaaa
     2341 aagcaggctg ccaccatggt cgacggcgga gactacaagg acgacgatga caagggcgga
     2401 ggcagcggag gaggatcaga acaccagctc ctgtgctgcg aagtggaaac catccgccgc
     2461 gcgtaccccg atgccaacct cctcaacgac cgggtgctgc gggccatgct gaaggcggag
     2521 gagacctgcg cgccctcggt gtcctacttc aaatgtgtgc agaaggaggt cctgccgtcc
     2581 atgcggaaga tcgtcgccac ctggatgctg gaggtctgcg aggaacagaa gtgcgaggag
     2641 gaggtcttcc cgctggccat gaactacctg gaccgcttcc tgtcgctgga gcccgtgaaa
     2701 aagagccgcc tgcagctgct gggggccact tgcatgttcg tggcctctaa gatgaaggag
     2761 accatccccc tgacggccga gaagctgtgc atctacaccg acaactccat ccggcccgag
     2821 gagctgctgc aaatggagct gctcctggtg aacaagctca agtggaacct ggccgcaatg
     2881 accccgcacg atttcattga acacttcctc tccaaaatgc cagaggcgga ggagaacaaa
     2941 cagatcatcc gcaaacacgc gcagaccttc gttgccctct gtgccacaga tgtgaagttc
     3001 atttccaatc cgccctccat ggtggcagcg gggagcgtgg tggccgcagt gcaaggcctg
     3061 aacctgagga gccccaacaa cttcctgtcc tactaccgcc tcacacgctt cctctccaga
     3121 gtgatcaagt gtgacccgga ctgcctccgg gcctgccagg agcagatcga agccctgctg
     3181 gagtcaagcc tgcgccaggc ccagcagaac atggacccca aggccgccga ggaggaggaa
     3241 gaggaggagg aggaggtgga cctggcttgc acacccaccg acgtgcggga cgtggacatc
     3301 tgagcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     3361 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     3421 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     3481 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     3541 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     3601 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     3661 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     3721 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     3781 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     3841 acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac catgggagga
     3901 ggcagcgcct ggagccaccc tcagttcgag aagggaggag gaagcggcgg aggcagcgga
     3961 ggaagcgcct ggagccaccc gcagttcgag aaaggaggag gagagctgct gtgccacgag
     4021 gtggacccgg tccgcagggc cgtgcgggac cgcaacctgc tccgagacga ccgcgtcctg
     4081 cagaacctgc tcaccatcga ggagcgctac cttccgcagt gctcctactt caagtgcgtg
     4141 cagaaggaca tccaacccta catgcgcaga atggtggcca cctggatgct ggaggtctgt
     4201 gaggaacaga agtgcgaaga agaggtcttc cctctggcca tgaattacct ggaccgtttc
     4261 ttggctgggg tcccgactcc gaagtcccat ctgcaactcc tgggtgctgt ctgcatgttc
     4321 ctggcctcca aactcaaaga gaccagcccg ctgaccgcgg agaagctgtg catttacacc
     4381 gacaactcca tcaagcctca ggagctgctg gagtgggaac tggtggtgct ggggaagttg
     4441 aagtggaacc tggcagctgt cactcctcat gacttcattg agcacatctt gcgcaagctg
     4501 ccccagcagc gggagaagct gtctctgatc cgcaagcatg ctcagacctt cattgctctg
     4561 tgtgccaccg actttaagtt tgccatgtac ccaccgtcga tgatcgcaac tggaagtgtg
     4621 ggagcagcca tctgtgggct ccagcaggat gaggaagtga gctcgctcac ttgtgatgcc
     4681 ctgactgagc tgctggctaa gatcaccaac acagacgtgg attgtctcaa agcttgccag
     4741 gagcagattg aggcggtgct cctcaatagc ctgcagcagt accgtcagga ccaacgtgac
     4801 ggatccaagt cggaggatga actggaccaa gccagcaccc ctacagacgt gcgggatatc
     4861 gacctgtgaa cccagctttc ttgtacaaag tgggcccctc tccctccccc ccccctaacg
     4921 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     4981 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     5041 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     5101 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     5161 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     5221 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     5281 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     5341 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     5401 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     5461 gatgataata tggccacaac catgggaggc ggatacccat acgacgtccc agactacgct
     5521 ggcggaggca gcggaggagg atcagagctg ctgtgttgcg aaggcacccg gcacgcgccc
     5581 cgggccgggc cggacccgcg gctgctgggg gaccagcgtg tcctgcagag cctgctccgc
     5641 ctggaggagc gctacgtacc ccgcgcctcc tacttccagt gcgtgcagcg ggagatcaag
     5701 ccgcacatgc ggaagatgct ggcttactgg atgctggagg tatgtgagga gcagcgctgt
     5761 gaggaggaag tcttccccct ggccatgaac tacctggatc gctacctgtc ttgcgtcccc
     5821 acccgaaagg cgcagttgca gctcctgggt gcggtctgca tgctgctggc ctccaagctg
     5881 cgcgagacca cgcccctgac catcgaaaaa ctgtgcatct acaccgacca cgctgtctct
     5941 ccccgccagt tgcgggactg ggaggtgctg gtcctaggga agctcaagtg ggacctggct
     6001 gctgtgattg cacatgattt cctggccttc attctgcacc ggctctctct gccccgtgac
     6061 cgacaggcct tggtcaaaaa gcatgcccag acctttttgg ccctctgtgc tacagattat
     6121 acctttgcca tgtacccgcc atccatgatc gccacgggca gcattggggc tgcagtgcaa
     6181 ggcctgggtg cctgctccat gtccggggat gagctcacag agctgctggc agggatcact
     6241 ggcactgaag tggactgcct gcgggcctgt caggagcaga tcgaagctgc actcagggag
     6301 agcctcaggg aagcctctca gaccagctcc agcccagcgc ccaaagcccc ccggggctcc
     6361 agcagccaag ggcccagcca gaccagcact cctacagatg tcacagccat acacctgtga
     6421 caactttatt atacatagtt gatcaattcc gataatcaac ctctggatta caaaatttgt
     6481 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6541 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6601 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6661 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6721 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6781 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     6841 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     6901 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     6961 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7021 tccctttggg ccgcctcccc gcatcgggaa ttcccgcggt tcgaattcta ccgggtaggg
     7081 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
     7141 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
     7201 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
     7261 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
     7321 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
     7381 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
     7441 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
     7501 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
     7561 ccgggccttt cgacctcacg tggccaccat gaccgagtac aagcccacgg tgcgcctcgc
     7621 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     7681 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     7741 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     7801 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     7861 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     7921 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     7981 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     8041 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     8101 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     8161 gtgcatgacc cgcaagcccg gtgcctgagg tacctttaag accaatgact tacaaggcag
     8221 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     8281 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     8341 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     8401 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     8461 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     8521 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     8581 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     8641 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     8701 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8761 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     8821 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     8881 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     8941 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     9001 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     9061 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     9121 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     9181 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     9241 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     9301 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     9361 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     9421 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     9481 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     9541 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     9601 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     9661 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     9721 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     9781 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     9841 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     9901 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     9961 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
    10021 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
    10081 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
    10141 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
    10201 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
    10261 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
    10321 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    10381 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    10441 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    10501 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    10561 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    10621 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    10681 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    10741 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    10801 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    10861 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    10921 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    10981 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    11041 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    11101 aagagagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    11161 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    11221 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    11281 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    11341 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    11401 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    11461 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
    11521 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    11581 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
    11641 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
    11701 accctcacta aagggaacaa aagctggagc tgcaagctt
//
