LOCUS       sequence-419617-        9777 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing BFP fused with CDK6 cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9777)
  AUTHORS   Aoki K, Hyuga M, Tarumoto Y, Nishibuchi G, Ueda A, Ochi Y, Sugino S,
            Mikami T, Kobushi H, Kato I, Akahane K, Inukai T, Takaori-Kondo A, 
            Takita J, Ogawa S, Yusa K
  TITLE     Canonical BAF complex regulates the oncogenic program in human 
            T-cell acute lymphoblastic leukemia.
  JOURNAL   Blood. 2023 Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857.
  PUBMED    37922452
REFERENCE   2  (bases 1 to 9777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2023
            Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857."
COMMENT     Sequence Label: pKLV-EF1a-BFPCDK6-W
FEATURES             Location/Qualifiers
     source          1..9777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..38
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        19..37
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        108..411
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        413..611
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     566..586
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             629..809
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    856..981
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1478..1711
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1896..1940
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2238..2355
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2826..4007
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3057..3999
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3956..3976
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4028..4726
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4745..4798
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(5845..5865)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6263..6274)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6726..6743)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6732..6956
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6982..7002)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6982..7000)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7021..7037)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7021..7037)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7034..7056)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7045..7061
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7069..7075,7076..7093,7094..7099))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7114..7135
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7252..7269)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7423..8011)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7503..7522)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8182..8973,8974..9042))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8805..8824
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9043..9147)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9173..9628)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9310..9331)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9522..9541
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9755..9777
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(9769..9777,1..9)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     join(9770..9777,1..9)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acggccagtg agcgcgcgta atacgactca ctatagggcg aattgactag ttattaatag
       61 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      121 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      181 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      241 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      301 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      361 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      421 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      481 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      541 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      601 tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
      661 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
      721 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
      781 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
      841 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
      901 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
      961 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     1021 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     1081 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     1141 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     1201 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     1261 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     1321 gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     1381 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     1441 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     1501 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     1561 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     1621 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     1681 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     1741 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     1801 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     1861 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     1921 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     1981 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     2041 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     2101 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     2161 acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg cagacaaatg
     2221 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2281 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2341 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gttagtaccg
     2401 ggccctacgc gtgaggggcg cggaagccgg ggcggaggag gagccaaccc cgggctgggc
     2461 tgagacccgc agaggaagac gctctaggga tttgtcccgg actagcgaga tggcaaggct
     2521 gaggacggga ggctgattga gaggcgaagg tacaccctaa tctcaataca acctttggag
     2581 ctaagccagc aatggtagag ggaagattct gcacgtccct tccaggcggc ctccccgtca
     2641 ccaccccccc caacccgccc cgaccggagc tgagagtaat tcatacaaaa ggactcgccc
     2701 ctgccttggg gaatcccagg gaccgtcgtt aaactcccac taacgtagaa cccagagatc
     2761 gctgcgttcc cgccccctca cccgcccgct ctcgtcatca ctgaggtgga gaagagcatg
     2821 cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
     2881 tggggggagg ggtcggcaat tgaaccggtg cctagagaaa gtggcgcggg gtaaactggg
     2941 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     3001 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
     3061 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
     3121 gaattacttc cacgcccctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
     3181 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
     3241 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
     3301 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
     3361 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
     3421 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
     3481 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
     3541 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
     3601 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
     3661 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
     3721 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
     3781 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
     3841 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     3901 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     3961 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtggga agcttggcgc
     4021 gcccaccatg agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac
     4081 cgtggacaac catcacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
     4141 ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct
     4201 ggctactagc ttcctctacg gcagcaagac cttcatcaac cacacccagg gcatccccga
     4261 cttcttcaag cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga
     4321 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa
     4381 cgtcaagatc agaggggtga acttcacatc caacggccct gtgatgcaga agaaaacact
     4441 cggctgggag gccttcaccg agacgctgta ccccgctgac ggcggcctgg aaggcagaaa
     4501 cgacatggcc ctgaagctcg tgggcgggag ccatctgatc gcaaacatca agaccacata
     4561 tagatccaag aaacccgcta agaacctcaa gatgcctggc gtctactatg tggactacag
     4621 actggaaaga atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt
     4681 ggccagatac tgcgacctcc ctagcaaact ggggcacaag cttaatggcg gcgggtccgg
     4741 aggagagggc agaggatctc ttctaacatg cggtgacgtg gaggagaatc ctggcccaat
     4801 ggagaaggac ggcctgtgcc gcgctgacca gcagtacgaa tgcgtggcgg agatcgggga
     4861 gggcgcctat gggaaggtgt tcaaggcccg cgacttgaag aacggaggcc gtttcgtggc
     4921 gttgaagcgc gtgcgggtgc agaccggcga ggagggcatg ccgctctcca ccatccgcga
     4981 ggtggcggtg ctgaggcacc tggagacctt cgagcacccc aacgtggtca ggttgtttga
     5041 tgtgtgcaca gtgtcacgaa cagacagaga aaccaaacta actttagtgt ttgaacatgt
     5101 cgatcaagac ttgaccactt acttggataa agttccagag cctggagtgc ccactgaaac
     5161 cataaaggat atgatgtttc agcttctccg aggtctggac tttcttcatt cacaccgagt
     5221 agtgcatcgc gatctaaaac cacagaacat tctggtgacc agcagcggac aaataaaact
     5281 cgctgacttc ggccttgccc gcatctatag tttccagatg gctctaacct cagtggtcgt
     5341 cacgctgtgg tacagagcac ccgaagtctt gctccagtcc agctacgcca cccccgtgga
     5401 tctctggagt gttggctgca tatttgcaga aatgtttcgt agaaagcctc tttttcgtgg
     5461 aagttcagat gttgatcaac taggaaaaat cttggacgtg attggactcc caggagaaga
     5521 agactggcct agagatgttg cccttcccag gcaggctttt cattcaaaat ctgcccaacc
     5581 aattgagaag tttgtaacag atatcgatga actaggcaaa gacctacttc tgaagtgttt
     5641 gacatttaac ccagccaaaa gaatatctgc ctacagtgcc ctgtctcacc catacttcca
     5701 ggacctggaa aggtgcaaag aaaacctgga ttcccacctg ccgcccagcc agaacacctc
     5761 ggagctgaat acagcctgag aattcgcggc cgctcaacct ctggattaca aaatttgtga
     5821 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     5881 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     5941 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6001 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6061 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6121 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6181 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6241 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6301 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     6361 cctttgggcc gcctccccgc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     6421 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaaagaag
     6481 tcaagatctg ctttttgcct gtactgggtc tctctggtta gaccagagtc tctctggtta
     6541 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     6601 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6661 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagttt aaacccgctg
     6721 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     6781 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     6841 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     6901 gggggaggat tgggaagtca atagcaggca tgctggggat gcggtgggct ctatgggcgg
     6961 ccgttaatta agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     7021 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7081 cgggagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7141 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7201 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7261 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7321 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7381 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7441 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7501 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7561 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7621 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7681 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7741 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7801 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7861 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7921 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7981 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8041 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8101 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8161 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8221 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8281 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8341 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8401 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8461 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8521 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8581 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8641 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8701 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8761 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     8821 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8881 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8941 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9001 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9061 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9121 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     9181 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     9241 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     9301 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     9361 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     9421 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     9481 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     9541 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     9601 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
     9661 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     9721 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacg
//
