LOCUS       sequence-41962-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a single-wavelength maltose sensor constructed from E. coli MalE and
            cpGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Marvin JS, Schreiter ER, Echevarria IM, Looger LL
  TITLE     A genetically encoded, high-signal-to-noise maltose sensor.
  JOURNAL   Proteins. 2011 Nov;79(11):3025-36. doi: 10.1002/prot.23118.
  PUBMED    21989929
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/prot"; 
            journalName: "Proteins"; date: "2011-11"; volume: "79"; issue: "11";
            pages: "3025-36"
COMMENT     Sequence Label: EcMBP165-cpGFP.PPYF.T203V.pRSET
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             64..96
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(100..108,109..123)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacat atgcggggtt ctcatcatca tcatcatcat
       61 ggtatggcta gcatgactgg tggacagcaa atgggtcggg atctgtacga cgatgacgat
      121 aaggatcgat ggggatccaa aatcgaagaa ggtaaactgg taatctggat taacggcgat
      181 aaaggctata acggtctcgc tgaagtcggt aagaaattcg agaaagatac cggaattaaa
      241 gtcaccgttg agcatccgga taaactggaa gagaaattcc cacaggttgc ggcaactggc
      301 gatggccctg acattatctt ctgggcacac gaccgctttg gtggctacgc tcaatctggc
      361 ctgttggctg aaatcacccc ggacaaagcg ttccaggaca agctgtatcc gtttacctgg
      421 gatgccgtac gttacaacgg caagctgatt gcttacccga tcgctgttga agcgttatcg
      481 ctgatttata acaaagatct gctgccgaac ccgccaaaaa cctgggaaga gatcccggcg
      541 ctggataaag aactgaaagc gaaaggtaag agcgcgctga tgttcaacct gcaagaaccg
      601 tacttcacct ggccgctgat tgctgctgac ccccccagct acaacgtctt catcatggcc
      661 gacaagcaga agaacggcat caaggcgaac ttcaagatcc gccacaacat cgaggacggc
      721 ggcgtgcagc tcgcctacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      781 ctgcccgaca accactacct gagcgt
//
