LOCUS       sequence-419620-       10457 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing mCherry fused with Luciferase at the 
            C-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10457)
  AUTHORS   Aoki K, Hyuga M, Tarumoto Y, Nishibuchi G, Ueda A, Ochi Y, Sugino S,
            Mikami T, Kobushi H, Kato I, Akahane K, Inukai T, Takaori-Kondo A, 
            Takita J, Ogawa S, Yusa K
  TITLE     Canonical BAF complex regulates the oncogenic program in human 
            T-cell acute lymphoblastic leukemia.
  JOURNAL   Blood. 2023 Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857.
  PUBMED    37922452
REFERENCE   2  (bases 1 to 10457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2023
            Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857."
COMMENT     Sequence Label: pKLV-EF1a-mCherryLuciferase-W
FEATURES             Location/Qualifiers
     source          1..10457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        81..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     539..559
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             602..782
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    829..954
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1451..1684
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1869..1913
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2211..2328
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2799..3980
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3030..3972
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3929..3949
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4000..4707
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNSAIIKEFLRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4145..4163)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4413..4432
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4726..4779
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4780..6432
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(6498..6518)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6916..6927)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7379..7396)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7385..7609
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7635..7655)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7635..7653)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7674..7690)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7674..7690)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7687..7709)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7698..7714
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7722..7728,7729..7746,7747..7752))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7767..7788
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7905..7922)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8076..8664)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8156..8175)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8835..9626,9627..9695))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9458..9477
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9696..9800)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9826..10281)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9963..9984)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10175..10194
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10408..10430
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10422..10439
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10423..10439
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(10449..10457,1..11)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(10449..10457,1..10)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcactatagg gcgaattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      421 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      481 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      541 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac
      601 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
      661 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
      721 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
      781 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
      841 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
      901 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
      961 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     1021 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     1081 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     1141 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     1201 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     1261 caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag
     1321 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     1381 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     1441 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     1501 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     1561 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     1621 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     1681 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     1741 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     1801 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     1861 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     1921 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     1981 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     2041 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     2101 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     2161 cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga
     2221 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2281 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     2341 agggacagca gagatccagt ttggttagta ccgggcccta cgcgtgaggg gcgcggaagc
     2401 cggggcggag gaggagccaa ccccgggctg ggctgagacc cgcagaggaa gacgctctag
     2461 ggatttgtcc cggactagcg agatggcaag gctgaggacg ggaggctgat tgagaggcga
     2521 aggtacaccc taatctcaat acaacctttg gagctaagcc agcaatggta gagggaagat
     2581 tctgcacgtc ccttccaggc ggcctccccg tcaccacccc ccccaacccg ccccgaccgg
     2641 agctgagagt aattcataca aaaggactcg cccctgcctt ggggaatccc agggaccgtc
     2701 gttaaactcc cactaacgta gaacccagag atcgctgcgt tcccgccccc tcacccgccc
     2761 gctctcgtca tcactgaggt ggagaagagc atgcgtgagg ctccggtgcc cgtcagtggg
     2821 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2881 gtgcctagag aaagtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2941 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     3001 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     3061 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacgccc ctggctgcag
     3121 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
     3181 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
     3241 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
     3301 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
     3361 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
     3421 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
     3481 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
     3541 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
     3601 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
     3661 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
     3721 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
     3781 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
     3841 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
     3901 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
     3961 cttccatttc aggtgtcgtg gaagcttggc gcgcccacca tggtgagcaa gggcgaggag
     4021 gataactccg ccatcatcaa ggagttcctg cgcttcaagg tgcacatgga gggctccgtg
     4081 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     4141 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     4201 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     4261 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     4321 ggcgtggtga ccgtgaccca ggactcctct ctgcaggacg gcgagttcat ctacaaggtg
     4381 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     4441 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     4501 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     4561 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     4621 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     4681 accggcggca tggacgagct gtataagggc ggcgggtccg gaggagaggg cagaggatct
     4741 cttctaacat gcggtgacgt ggaggagaat cctggcccaa tggaagatgc caaaaacatt
     4801 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac
     4861 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc
     4921 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg
     4981 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag
     5041 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac
     5101 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc
     5161 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa
     5221 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc
     5281 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac
     5341 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc
     5401 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc
     5461 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc
     5521 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc
     5581 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg
     5641 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc
     5701 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg
     5761 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc
     5821 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc
     5881 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc
     5941 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct
     6001 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac
     6061 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac
     6121 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc
     6181 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg
     6241 gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca
     6301 accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc
     6361 ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag
     6421 atcgccgtgt aagaattcgc ggccgctcaa cctctggatt acaaaatttg tgaaagattg
     6481 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6541 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     6601 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     6661 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     6721 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     6781 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     6841 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     6901 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     6961 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     7021 gccgcctccc cgcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     7081 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaaag aagtcaagat
     7141 ctgctttttg cctgtactgg gtctctctgg ttagaccaga gtctctctgg ttagaccaga
     7201 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7261 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7321 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tttaaacccg ctgatcagcc
     7381 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     7441 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     7501 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     7561 gattgggaag tcaatagcag gcatgctggg gatgcggtgg gctctatggg cggccgttaa
     7621 ttaagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
     7681 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccgggagc
     7741 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7801 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7861 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7921 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7981 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8041 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8101 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8161 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8221 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8281 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8341 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8401 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8461 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     8521 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8581 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8641 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8701 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8761 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8821 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8881 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8941 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9001 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9061 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9121 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9181 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9241 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9301 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9361 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9421 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9481 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9541 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9601 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9661 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9721 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9781 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta
     9841 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     9901 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     9961 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
    10021 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
    10081 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
    10141 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
    10201 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
    10261 atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc
    10321 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
    10381 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
    10441 agcgcgcgta atacgac
//
