LOCUS       sequence-419621-        9032 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing gRNA targeting human ARID1A and ARID1B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9032)
  AUTHORS   Aoki K, Hyuga M, Tarumoto Y, Nishibuchi G, Ueda A, Ochi Y, Sugino S,
            Mikami T, Kobushi H, Kato I, Akahane K, Inukai T, Takaori-Kondo A, 
            Takita J, Ogawa S, Yusa K
  TITLE     Canonical BAF complex regulates the oncogenic program in human 
            T-cell acute lymphoblastic leukemia.
  JOURNAL   Blood. 2023 Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857.
  PUBMED    37922452
REFERENCE   2  (bases 1 to 9032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2023
            Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857."
COMMENT     Sequence Label: 
            pKLV2.2-h7SKgRNA5(ARID1A(44))-hU6gRNA5(ARID1B(23))-PGKpuroBFP-W
FEATURES             Location/Qualifiers
     source          1..9032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(22..477)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(159..180)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     371..390
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     604..626
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     618..635
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     619..635
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     645..664
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        645..663
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        979..1177
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1132..1152
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1195..1375
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1422..1547
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2046..2279
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2464..2508
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2806..2923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3380..3628
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3380..3400
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3551..3570
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        3757..4256
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3792..3815)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4189..4208
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4296..4892
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4296..4315)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4792..4812
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4911..4964
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4965..5666
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(5726..5746)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6144..6155)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6607..6624)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6613..6837
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6863..6883)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6863..6881)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6902..6918)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6902..6918)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6915..6937)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6926..6942
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6950..6956,6957..6974,6975..6980))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6995..7016
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7133..7150)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7304..7892)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7384..7403)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8063..8854,8855..8923))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8686..8705
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8924..9028)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
       61 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
      121 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
      181 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
      241 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
      301 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
      361 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
      421 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
      481 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      541 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      601 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
      661 agggcgaatt gactagtaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      721 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      781 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      841 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      901 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      961 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1021 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1081 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1141 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
     1201 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     1261 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     1321 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1381 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1441 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1501 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1561 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1621 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1681 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1741 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1801 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa cagcaaaaca
     1861 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga
     1921 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1981 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     2041 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     2101 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     2161 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     2221 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     2281 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     2341 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     2401 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     2461 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     2521 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     2581 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2641 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2701 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     2761 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2821 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2881 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2941 cagcagagat ccagtttggt tagtaccggg ccctacgcgt ctgcagtatt tagcatgccc
     3001 cacccatctg caaggcattc tggatagtgt caaaacagcc ggaaatcaag tccgtttatc
     3061 tcaaacttta gcattttggg aataaatgat atttgctatg ctggttaaat tagattttag
     3121 ttaaatttcc tgctgaagct ctagtacgat aagcaacttg acctaagtgt aaagttgaga
     3181 cttccttcag gtttatatag cttgtgcgcc gcttgggtac ctcgagggca aactgcccag
     3241 tgtgtttaag agctatgctg gaaacagcat agcaagttta aataaggcta gtccgttatc
     3301 aacttgaaaa agtggcaccg agtcggtgct ttttttagcc gaactgtttc acactcacgc
     3361 gtccaaggtc gggcaggaag agggcctatt tcccatgatt ccttcatatt tgcatatacg
     3421 atacaaggct gttagagaga taattagaat taatttgact gtaaacacaa agatattagt
     3481 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
     3541 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
     3601 atatatcttg tggaaaggac gaaacaccgt ttgacgcaga cgtagagtgt ttaagagcta
     3661 tgctggaaac agcatagcaa gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg
     3721 caccgagtcg gtgctttttt tggatccaat tctaccgggt aggggaggcg cttttcccaa
     3781 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
     3841 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
     3901 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
     3961 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
     4021 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
     4081 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
     4141 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
     4201 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct
     4261 gcatccatct agatctcgag cagctgaagc ttaccatgac cgagtacaag cccacggtgc
     4321 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     4381 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     4441 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     4501 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     4561 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     4621 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     4681 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     4741 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     4801 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     4861 gcacctggtg catgacccgc aagcccggtg ccggcggcgg gtccggagga gagggcagag
     4921 gaagtctcct aacatgcggt gacgtggagg agaatcctgg cccaatgagc gagctgatta
     4981 aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat cacttcaagt
     5041 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg
     5101 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc ctctacggca
     5161 gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag tccttccctg
     5221 agggcttcac atgggagaga gtcaccacat acgaggacgg gggcgtgctg accgctaccc
     5281 aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact
     5341 tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga
     5401 cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg aagctcgtgg
     5461 gcgggagcca tctgatcgca aacatcaaga ccacatatag atccaagaaa cccgctaaga
     5521 acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc aaggaggcca
     5581 acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatactgc gacctcccta
     5641 gcaaactggg gcacaagctt aattgagcgg ccgctcaacc tctggattac aaaatttgtg
     5701 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     5761 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     5821 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     5881 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     5941 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     6001 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     6061 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     6121 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     6181 tgctgccggc tctgcggcct cttccgcgtc tacgccttcg ccctcagacg agtcggatct
     6241 ccctttgggc cgcctccccg caggtacctt taagaccaat gacttacaag gcagctgtag
     6301 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaaagaa
     6361 gtcaagatct gctttttgcc tgtactgggt ctctctggtt agaccagagt ctctctggtt
     6421 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6481 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6541 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtt taaacccgct
     6601 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     6661 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     6721 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     6781 agggggagga ttgggaagtc aatagcaggc atgctgggga tgcggtgggc tctatgggcg
     6841 gccgttaatt aagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     6901 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6961 ccgggagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7021 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7081 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctgttccgct tcctcgctca
     7141 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7201 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7261 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7321 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7381 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7441 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7501 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7561 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7621 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7681 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7741 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7801 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7861 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7921 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7981 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8041 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8101 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8161 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8221 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8281 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8341 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8401 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8461 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8521 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8581 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8641 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8701 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8761 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8821 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8881 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8941 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9001 agaaaaataa acaaataggg gttccgcgca ca
//
