LOCUS       sequence-419635-        8650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing gRNA targeting human BCL11B-E2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Aoki K, Hyuga M, Tarumoto Y, Nishibuchi G, Ueda A, Ochi Y, Sugino S,
            Mikami T, Kobushi H, Kato I, Akahane K, Inukai T, Takaori-Kondo A, 
            Takita J, Ogawa S, Yusa K
  TITLE     Canonical BAF complex regulates the oncogenic program in human 
            T-cell acute lymphoblastic leukemia.
  JOURNAL   Blood. 2023 Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857.
  PUBMED    37922452
REFERENCE   2  (bases 1 to 8650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2023
            Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857."
COMMENT     Sequence Label: pKLV2-U6gRNA5(BCL11B-E2(24))-PGKpuro2ABFP-W
FEATURES             Location/Qualifiers
     source          1..8650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        254..452
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     407..427
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             470..650
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    697..822
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1321..1554
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1739..1783
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2081..2198
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2273..2521
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2273..2293
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2444..2463
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2650..3149
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2685..2708)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3082..3101
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3189..3785
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3189..3208)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3685..3705
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3804..3857
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3858..4559
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(4619..4639)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5037..5048)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5500..5517)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5506..5730
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5756..5776)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5756..5774)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5795..5811)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5795..5811)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5808..5830)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5819..5835
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5843..5849,5850..5867,5868..5873))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5888..5909
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6026..6043)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6197..6785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6277..6296)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6956..7747,7748..7816))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7579..7598
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7817..7921)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7947..8402)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8084..8105)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8296..8315
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8529..8551
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8543..8560
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8544..8560
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8570..8589
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8570..8588
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
       61 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      121 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      181 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      241 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      301 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      361 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      421 aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg
      481 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      541 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      601 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      661 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      721 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      781 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      841 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      901 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      961 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1021 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1081 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1141 aagaccaccg cacagcaagc ggccggccgc gctgatcttc agacctggag gaggagatat
     1201 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1261 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1321 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1381 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1441 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1501 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1561 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1621 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1681 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1741 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     1801 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     1861 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     1921 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     1981 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg
     2041 tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     2101 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     2161 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     2221 gagatccagt ttggttagta ccgggcccta cgcgtccaag gtcgggcagg aagagggcct
     2281 atttcccatg attccttcat atttgcatat acgatacaag gctgttagag agataattag
     2341 aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata
     2401 atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac
     2461 cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac
     2521 cgcctttttc tcccgcggtt cgtttaagag ctatgctgga aacagcatag caagtttaaa
     2581 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttggatcc
     2641 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     2701 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
     2761 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     2821 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     2881 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     2941 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
     3001 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
     3061 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     3121 tctcctcttc ctcatctccg ggcctttcga cctgcatcca tctagatctc gagcagctga
     3181 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     3241 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     3301 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     3361 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     3421 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     3481 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     3541 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     3601 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     3661 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     3721 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     3781 gtgccggcgg cgggtccgga ggagagggca gaggaagtct cctaacatgc ggtgacgtgg
     3841 aggagaatcc tggcccaatg agcgagctga ttaaggagaa catgcacatg aagctgtaca
     3901 tggagggcac cgtggacaac catcacttca agtgcacatc cgagggcgaa ggcaagccct
     3961 acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct
     4021 tcgacatcct ggctactagc ttcctctacg gcagcaagac cttcatcaac cacacccagg
     4081 gcatccccga cttcttcaag cagtccttcc ctgagggctt cacatgggag agagtcacca
     4141 catacgagga cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc
     4201 tcatctacaa cgtcaagatc agaggggtga acttcacatc caacggccct gtgatgcaga
     4261 agaaaacact cggctgggag gccttcaccg agacgctgta ccccgctgac ggcggcctgg
     4321 aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag ccatctgatc gcaaacatca
     4381 agaccacata tagatccaag aaacccgcta agaacctcaa gatgcctggc gtctactatg
     4441 tggactacag actggaaaga atcaaggagg ccaacaacga gacctacgtc gagcagcacg
     4501 aggtggcagt ggccagatac tgcgacctcc ctagcaaact ggggcacaag cttaattgag
     4561 cggccgctca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     4621 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4681 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     4741 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     4801 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     4861 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     4921 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     4981 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     5041 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     5101 gtctacgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcaggtac
     5161 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     5221 ggggactgga agggctaatt cactcccaaa gaagtcaaga tctgcttttt gcctgtactg
     5281 ggtctctctg gttagaccag agtctctctg gttagaccag atctgagcct gggagctctc
     5341 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     5401 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     5461 agtgtggaaa atctctagca gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     5521 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     5581 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     5641 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gtcaatagca
     5701 ggcatgctgg ggatgcggtg ggctctatgg gcggccgtta attaagcttt tgttcccttt
     5761 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     5821 gttatccgct cacaattcca cacaacatac gagccgggag cataaagtgt aaagcctggg
     5881 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     5941 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6001 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6061 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6121 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6181 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6241 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6301 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6361 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6421 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6481 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6541 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6601 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     6661 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6721 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6781 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6841 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6901 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6961 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7021 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7081 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7141 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7201 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7261 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7321 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7381 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7441 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7501 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7561 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7621 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7681 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7741 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7801 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7861 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7921 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
     7981 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     8041 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     8101 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     8161 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     8221 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     8281 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     8341 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     8401 gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     8461 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     8521 agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc
     8581 actatagggc gaattgacta gtacataact tacggtaaat ggcccgcctg gctgaccgcc
     8641 caacgacccc
//
