LOCUS       sequence-419669-        1963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  (Empty Backbone) pCOLA-Spec Empty Vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Hennigan JN, Menacho-Melgar R, Sarkar P, Golovsky M, Lynch MD
  TITLE     
  JOURNAL   Metab Eng. 2024 Sep;85:116-130. doi: 10.1016/j.ymben.2024.07.012. 
            Epub 2024 Jul 24.
  PUBMED    39059674
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2024-09"; volume: "85:116-130. doi"; pages: " 
            10.1016/j.ymben.2024.07.012. Epub 2024 Jul 24"
COMMENT     Sequence Label: pCOLA-Spec-EV
FEATURES             Location/Qualifiers
     source          1..1963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(181..816)
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin of replication 
                     can be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     primer_bind     complement(326..345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      1025..1052
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        1058..1148
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1163..1954
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gaagtgccag acgatggaag gacgcaatga agctggagga ttggcgtggg aatcgtgctt
       61 ctgtctaagc aagaatgcct agcgtacagg gtgcactttg taacgatttg ggagtccaga
      121 gactcgctgt tttcgaaatt ctcggtcgct acgctccggg cgtgagacta tgcagcgcag
      181 aaacgtccta gaagatgcca ggaggatact tagcagagag acaataaggc cggagcgaag
      241 ccgtttttcc ataggctccg cccccctgac gaacatcacg aaatctgacg ctcaaatcag
      301 tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctga tggctccctc
      361 ttgcgctctc ctgttcccgt cctgcggcgt ccgtgttgtg gtggaggctt tacccaaatc
      421 accacgtccc gttccgtgta gacagttcgc tccaagctgg gctgtgtgca agaacccccc
      481 gttcagcccg actgctgcgc cttatccggt aactatcatc ttgagtccaa cccggaaaga
      541 cacgacaaaa cgccactggc agcagccatt ggtaactgag aattagtgga tttagatatc
      601 gagagtcttg aagtggtggc ctaacagagg ctacactgaa aggacagtat ttgatatctg
      661 cgctccacta aagccagtta ccaggttaag cagttcccca actgacttaa ccttcgatca
      721 aaccgcctcc ccaggcggtt ttttcgttta cagagcagga gattacgacg atcgtaaaag
      781 gatctcaaga agatccttta cggattcccg acaccatcac tctagatttc agtgcaattt
      841 atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgta attctcatgt
      901 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg
      961 gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgtgg atccgagtca
     1021 cactggctca ccttcgggtg ggcctttctg cgtttattga tcggcacgta agaggttcca
     1081 actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt
     1141 caggagctgg aaggaagtaa aaatgaggga agcggtgatc gccgaagtat cgactcaact
     1201 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     1261 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     1321 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     1381 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     1441 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     1501 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     1561 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     1621 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     1681 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     1741 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     1801 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     1861 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     1921 gaaaggcgag atcaccaagg tagtcggcaa ataatagaag ctt
//
