LOCUS       sequence-419670-        2693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sulfydryl oxidase (Erv1p) after phosphate depletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Hennigan JN, Menacho-Melgar R, Sarkar P, Golovsky M, Lynch MD
  TITLE     
  JOURNAL   Metab Eng. 2024 Sep;85:116-130. doi: 10.1016/j.ymben.2024.07.012. 
            Epub 2024 Jul 24.
  PUBMED    39059674
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2024-09"; volume: "85:116-130. doi"; pages: " 
            10.1016/j.ymben.2024.07.012. Epub 2024 Jul 24"
COMMENT     Sequence Label: pCOLA-Spec-yibDp-Erv1p
FEATURES             Location/Qualifiers
     source          1..2693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(180..815)
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin of replication 
                     can be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     primer_bind     complement(325..344)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      1754..1781
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        1787..1877
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1892..2683
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aagtgccaga cgatggaagg acgcaatgaa gctggaggat tggcgtggga atcgtgcttc
       61 tgtctaagca agaatgccta gcgtacaggg tgcactttgt aacgatttgg gagtccagag
      121 actcgctgtt ttcgaaattc tcggtcgcta cgctccgggc gtgagactat gcagcgcaga
      181 aacgtcctag aagatgccag gaggatactt agcagagaga caataaggcc ggagcgaagc
      241 cgtttttcca taggctccgc ccccctgacg aacatcacga aatctgacgc tcaaatcagt
      301 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgat ggctccctct
      361 tgcgctctcc tgttcccgtc ctgcggcgtc cgtgttgtgg tggaggcttt acccaaatca
      421 ccacgtcccg ttccgtgtag acagttcgct ccaagctggg ctgtgtgcaa gaaccccccg
      481 ttcagcccga ctgctgcgcc ttatccggta actatcatct tgagtccaac ccggaaagac
      541 acgacaaaac gccactggca gcagccattg gtaactgaga attagtggat ttagatatcg
      601 agagtcttga agtggtggcc taacagaggc tacactgaaa ggacagtatt tgatatctgc
      661 gctccactaa agccagttac caggttaagc agttccccaa ctgacttaac cttcgatcaa
      721 accgcctccc caggcggttt tttcgtttac agagcaggag attacgacga tcgtaaaagg
      781 atctcaagaa gatcctttac ggattcccga caccatcact ctagatttca gtgcaattta
      841 tctcttcaaa tgtagcacct gaagtcagcc ccatacgata taagttgtaa ttctcatgtt
      901 agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg
      961 tcgagatccc ggtgcctaat gagtgagcta acttacatta attgcgtgtg cgtaattgtg
     1021 ctgatctctt atatagctgc tctcattatc tctctaccct gaagtgactc tctcacctgt
     1081 aaaaataata tctcacaggc ttaatagttt cttaatacaa agcctgtaaa acgtcaggat
     1141 aacttctgtg taggaggata atctatgaag gcaattgata agatgaccga caatcctcct
     1201 caggaaggct tgtccgggcg caaaatcatc tatgatgagg acgggaagcc gtgccgttcc
     1261 tgcaatactt tgctggactt ccaatacgtc acgggtaaaa tctcgaatgg attgaagaat
     1321 ttgtcttcaa atggaaaact ggcagggact ggggctctta cgggggaggc atcggaactt
     1381 atgcctggtt cccgcactta tcgcaaagta gatccacctg atgttgagca gcttggccgt
     1441 tcttcgtgga ccttgttgca ctctgtcgcc gcgtcgtatc cagctcagcc tacggaccaa
     1501 cagaagggcg agatgaaaca gttcctgaac atcttctcgc acatttaccc ttgcaactgg
     1561 tgcgccaagg acttcgagaa gtacattcgc gagaatgcgc cgcaagtaga atcccgcgaa
     1621 gaattgggac gttggatgtg tgaagcacat aataaggtga ataagaagtt acgcaaacca
     1681 aagttcgact gtaatttctg ggaaaaacgc tggaaagacg ggtgggacga gtaatgagga
     1741 tccgagtcac actggctcac cttcgggtgg gcctttctgc gtttattgat cggcacgtaa
     1801 gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat
     1861 cgagattttc aggagctgga aggaagtaaa aatgagggaa gcggtgatcg ccgaagtatc
     1921 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
     1981 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt
     2041 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
     2101 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
     2161 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
     2221 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
     2281 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
     2341 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
     2401 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     2461 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     2521 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     2581 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     2641 ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatagaagc ttg
//
