LOCUS       sequence-419674-       10351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cRaf fused to miRFP703-eDHFR(69K6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10351)
  AUTHORS   Deguchi E, Lin S, Hirayama D, Matsuda K, Tanave A, Sumiyama K, 
            Tsukiji S, Otani T, Furuse M, Sorkin A, Matsuda M, Terai K
  TITLE     Low-affinity ligands of the epidermal growth factor receptor are 
            long-range signal transmitters in collective cell migration of 
            epithelial cells.
  JOURNAL   Cell Rep. 2024 Nov 14;43(11):114986. doi: 
            10.1016/j.celrep.2024.114986.
  PUBMED    39546398
REFERENCE   2  (bases 1 to 10351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-11-14"; volume: "43(11):114986. doi"; pages: " 
            10.1016/j.celrep.2024.114986"
COMMENT     Sequence Label: pPBbsr2-miRFP703-eDHFR(69K6)-cRaf
FEATURES             Location/Qualifiers
     source          1..10351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1392..1669
                     /label=chicken beta-actin promoter
     intron          1670..2686
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2610..2632
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2694..2713
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2810..2819
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2816..3760
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 703 nm (Shcherbakova et al., 2016)"
                     /label=miRFP703
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSLVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPIHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     misc_feature    6415..6964
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6559..6576)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6786..6805
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(7435..7452)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7441..7665
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7712..7728)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7736..7752
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7760..7766,7767..7784,7785..7790))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7805..7826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   8060..8122
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8163..8183)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8163..8181)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8202..8218)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8226..8242
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8250..8256,8257..8274,8275..8280))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8295..8316
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8433..8450)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8604..9192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8684..8703)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9363..10154,10155..10223))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9986..10005
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10224..10328)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tggggggggg
     1501 gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag
     1561 gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc
     1621 gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
     1681 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
     1741 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa
     1801 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
     1861 gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg
     1921 cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg
     1981 cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc
     2041 gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
     2101 gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg
     2161 agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct
     2221 cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg
     2281 ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg
     2341 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
     2401 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
     2461 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
     2521 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
     2581 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
     2641 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     2701 gctggttatt gtgctgtctc atcattttgg caaagaattg ggatccgaat tcctcgaggg
     2761 cggccgctct agagtcgacg ggccgcggta acaattgtta acaccggtcg ccaccatggt
     2821 agcaggtcat gcctctggca gccccgcatt cgggaccgcc tctcattcga attgcgaaca
     2881 tgaagagatc cacctcgccg gctcgatcca gccgcatggc gcgcttctgg tcgtcagcga
     2941 acatgatcat cgcgtcatcc aggccagcgc caacgccgcg gaatttctga atctcggaag
     3001 cgtactcggc gttccgctcg ccgagatcga cggcgatctg ttgatcaaga tcctgccgca
     3061 tctcgatccc accgccgaag gcatgccggt cgcggtgcgc tgccggatcg gcaatccctc
     3121 tacggagtac tgcggtctga tgcatcggcc tccggaaggc gggctgatca tcgaactcga
     3181 acgtgccggc ccgtcgatcg atctgtcagg cacgctggcg ccggcgctgg agcggatccg
     3241 cacggcgggt tcactgcgcg cgctgtgcga tgacaccgtg ctgctgtttc agcagtgcac
     3301 cggctacgac cgggtgatgg tgtatcgttt cgatgagcaa ggccacggcc tggtattctc
     3361 cgagtgccat gtgcctgggc tcgaatccta tttcggcaac cgctatccgt cgtcgctggt
     3421 cccgcagatg gcgcggcagc tgtacgtgcg gcagcgcgtc cgcgtgctgg tcgacgtcac
     3481 ctatcagccg gtgccgctgg agccgcggct gtcgccgctg accgggcgcg atctcgacat
     3541 gtcgggctgc ttcctgcgct cgatgtcgcc gatccatctg cagttcctga aggacatggg
     3601 cgtgcgcgcc accctggcgg tgtcgctggt ggtcggcggc aagctgtggg gcctggttgt
     3661 ctgtcaccat tatctgccgc gcttcatccg tttcgagctg cgggcgatct gcaaacggct
     3721 cgccgaaagg atcgcgacgc ggatcaccgc gcttgagagc ctgtacaagt ctgggtccgc
     3781 agccagtagc gccggaagta tgatcagtct gattgcggcg ttagcggtag atcgcgttat
     3841 cggcatggaa aacgccatgc cgtggaacct gcctgccgat ctcgcctggt ttaaacgcaa
     3901 caccttaaat aaacccgtga ttatgggccg ccatacctgg gaatcaatcg gtcgtccgtt
     3961 gccaggacgc aaaaatatta tcctcagcag tcaaccgggt acggacaaaa aaaagaaaaa
     4021 gaaagatcgc gtaacgtggg tgaagtcggt ggatgaagcc atcgcggcgt gtggtgacgt
     4081 accagaaatc atggtgattg gcggcggtcg cgtttatgaa cagttcttgc caaaagcgca
     4141 aaaactgtat ctgacgcata tcgacgcaga agtggaaggc gacacccatt tcccggatta
     4201 cgagccggat gactgggaat cggtattcag cgaattccac gatgctgatg cgcagaactc
     4261 tcacagctat tgctttgaga ttctggagcg gcggagtgct ggtggtagtg ctggtggtag
     4321 tgctggtggt agtgctggtg gtagtgctgg tggtcctcga agcctcgaga tggagcacat
     4381 acagggagct tggaagacga tcagcaatgg ttttggattc aaagatgccg tgtttgatgg
     4441 ctccagctgc atctctccta caatagttca gcagtttggc tatcagcgcc gggcatcaga
     4501 tgatggcaaa ctcacagatc cttctaagac aagcaacact atccgtgttt tcttgccgaa
     4561 caagcaaaga acagtggtca atgtgcgaaa tggaatgagc ttgcatgact gccttatgaa
     4621 agcactcaag gtgaggggcc tgcaaccaga gtgctgtgca gtgttcagac ttctccacga
     4681 acacaaaggt aaaaaagcac gcttagattg gaatactgat gctgcgtctt tgattggaga
     4741 agaacttcaa gtagatttcc tggatcatgt tcccctcaca acacacaact ttgctcggaa
     4801 gacgttcctg aagcttgcct tctgtgacat ctgtcagaaa ttcctgctca atggatttcg
     4861 atgtcagact tgtggctaca aatttcatga gcactgtagc accaaagtac ctactatgtg
     4921 tgtggactgg agtaacatca gacaactctt attgtttcca aattccacta ttggtgatag
     4981 tggagtccca gcactacctt ctttgactat gcgtcgtatg cgagagtctg tttccaggat
     5041 gcctgttagt tctcagcaca gatattctac acctcacgcc ttcaccttta acacctccag
     5101 tccctcatct gaaggttccc tctcccagag gcagaggtcg acatccacac ctaatgtcca
     5161 catggtcagc accaccctgc ctgtggacag caggatgatt gaggatgcaa ttcgaagtca
     5221 cagcgaatca gcctcacctt cagccctgtc cagtagcccc aacaatctga gcccaacagg
     5281 ctggtcacag ccgaaaaccc ccgtgccagc acaaagagag cgggcaccag tatctgggac
     5341 ccaggagaaa aacaaaatta ggcctcgtgg acagagagat tcaagctatt attgggaaat
     5401 agaagccagt gaagtgatgc tgtccactcg gattgggtca ggctcttttg gaactgttta
     5461 taagggtaaa tggcacggag atgttgcagt aaagatccta aaggttgtcg acccaacccc
     5521 agagcaattc caggccttca ggaatgaggt ggctgttctg cgcaaaacac ggcatgtgaa
     5581 cattctgctt ttcatggggt acatgacaaa ggacaacctg gcaattgtga cccagtggtg
     5641 cgagggcagc agcctctaca aacacctgca tgtccaggag accaagtttc agatgttcca
     5701 gctaattgac attgcccggc agacggctca gggaatggac tatttgcatg caaagaacat
     5761 catccataga gacatgaaat ccaacaatat atttctccat gaaggcttaa cagtgaaaat
     5821 tggagatttt ggtttggcaa cagtaaagtc acgctggagt ggttctcagc aggttgaaca
     5881 acctactggc tctgtcctct ggatggcccc agaggtgatc cgaatgcagg ataacaaccc
     5941 attcagtttc cagtcggatg tctactccta tggcatcgta ttgtatgaac tgatgacggg
     6001 ggagcttcct tattctcaca tcaacaaccg agatcagatc atcttcatgg tgggccgagg
     6061 atatgcctcc ccagatctta gtaagctata taagaactgc cccaaagcaa tgaagaggct
     6121 ggtagctgac tgtgtgaaga aagtaaagga agagaggcct ctttttcccc agatcctgtc
     6181 ttccattgag ctgctccaac actctctacc gaagatcaac cggagcgctt ccgagccatc
     6241 cttgcatcgg gcagcccaca ctgaggatat caatgcttgc acgctgacca cgtccccgag
     6301 gctgcctgtc ttctgcggcc gctagtcgac ggtaccgcgg gcccgggata agtcaactaa
     6361 cttaagctag caacggtttc cctctagcgg gatcaattcc gccccccccc cctaacgtta
     6421 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     6481 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     6541 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     6601 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     6661 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     6721 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     6781 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     6841 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     6901 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     6961 aataccatgg tcatgaaaac atttaacatt tctcaacaag atctagaatt agtagaagta
     7021 gcgacagaga agattacaat gctttatgag gataataaac atcatgtggg agcggcaatt
     7081 cgtacgaaaa caggagaaat catttcggca gtacatattg aagcgtatat aggacgagta
     7141 actgtttgtg cagaagccat tgcgattggt agtgcagttt cgaatggaca aaaggatttt
     7201 gacacgattg tagctgttag acacccttat tctgacgaag tagatagaag tattcgagtg
     7261 gtaagtcctt gtggtatgtg tagggagttg atttcagact atgcaccaga ttgttttgtg
     7321 ttaatagaaa tgaatggcaa gttagtcaaa actacgattg aagaactcat tccactcaaa
     7381 tatacccgaa attaaaagtc gagatgcatc gatgatctag agctcgctga tcagcctcga
     7441 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     7501 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     7561 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     7621 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     7681 gaacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7741 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7801 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7861 gaaacctgtc gtgccagcgg atcttaaaag ttttgttact ttatagaaga aattttgagt
     7921 ttttgttttt ttttaataaa taaataaaca taaataaatt gtttgttgaa tttattatta
     7981 gtatgtaagt gtaaatataa taaaacttaa tatctattca aattaataaa taaacctcga
     8041 tatacagacc gataaaacac atgcgtcaat tttacgcatg attatcttta acgtacgtca
     8101 caatatgatt atctttctag ggttaatcta gtatacgcgt ttaattaatc cagcttttgt
     8161 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     8221 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     8281 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     8341 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     8401 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     8461 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     8521 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     8581 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     8641 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     8701 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     8761 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     8821 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     8881 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     8941 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     9001 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     9061 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     9121 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     9181 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     9241 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     9301 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     9361 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     9421 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     9481 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     9541 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     9601 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     9661 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     9721 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     9781 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     9841 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     9901 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9961 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    10021 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    10081 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    10141 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    10201 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    10261 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    10321 gttccgcgca catttccccg aaaagtgcca c
//
