LOCUS       sequence-419676-       10340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cRaf fused to miRFP703-eDHFR(69K6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10340)
  AUTHORS   Deguchi E, Lin S, Hirayama D, Matsuda K, Tanave A, Sumiyama K, 
            Tsukiji S, Otani T, Furuse M, Sorkin A, Matsuda M, Terai K
  TITLE     Low-affinity ligands of the epidermal growth factor receptor are 
            long-range signal transmitters in collective cell migration of 
            epithelial cells.
  JOURNAL   Cell Rep. 2024 Nov 14;43(11):114986. doi: 
            10.1016/j.celrep.2024.114986.
  PUBMED    39546398
REFERENCE   2  (bases 1 to 10340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-11-14"; volume: "43(11):114986. doi"; pages: " 
            10.1016/j.celrep.2024.114986"
COMMENT     Sequence Label: pPBbleo-miRFP703-eDHFR(69K6)-cRaf
FEATURES             Location/Qualifiers
     source          1..10340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1713..2729
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2653..2675
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2737..2756
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2853..2862
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2859..3803
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 703 nm (Shcherbakova et al., 2016)"
                     /label=miRFP703
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSLVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPIHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     misc_feature    6458..7007
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6602..6619)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6829..6848
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7010..7384
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(7424..7441)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7430..7654
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7701..7717)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7725..7741
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7749..7755,7756..7773,7774..7779))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7794..7815
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   8049..8111
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8152..8172)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8152..8170)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8191..8207)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8215..8231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8239..8245,8246..8263,8264..8269))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8284..8305
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8422..8439)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8593..9181)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8673..8692)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9352..10143,10144..10212))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9975..9994
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10213..10317)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg ggcgcgcgcc aggcgggggg gggggggggg gggggggggg gggggccgcg
     1561 ggcggggggg gggggggggg ggcgaggggg ggggcggggc gaggcggaga ggtgcggcgg
     1621 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc
     1681 ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg
     1741 tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc
     1801 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat
     1861 gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt
     1921 tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt
     1981 gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc
     2041 tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct
     2101 gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg
     2161 gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc
     2221 cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg
     2281 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg
     2341 gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc
     2401 attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg
     2461 cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt
     2521 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc
     2581 cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac
     2641 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc
     2701 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt
     2761 ctcatcattt tggcaaagaa ttgggatccg aattcctcga gggcggccgc tctagagtcg
     2821 acgggccgcg gtaacaattg ttaacaccgg tcgccaccat ggtagcaggt catgcctctg
     2881 gcagccccgc attcgggacc gcctctcatt cgaattgcga acatgaagag atccacctcg
     2941 ccggctcgat ccagccgcat ggcgcgcttc tggtcgtcag cgaacatgat catcgcgtca
     3001 tccaggccag cgccaacgcc gcggaatttc tgaatctcgg aagcgtactc ggcgttccgc
     3061 tcgccgagat cgacggcgat ctgttgatca agatcctgcc gcatctcgat cccaccgccg
     3121 aaggcatgcc ggtcgcggtg cgctgccgga tcggcaatcc ctctacggag tactgcggtc
     3181 tgatgcatcg gcctccggaa ggcgggctga tcatcgaact cgaacgtgcc ggcccgtcga
     3241 tcgatctgtc aggcacgctg gcgccggcgc tggagcggat ccgcacggcg ggttcactgc
     3301 gcgcgctgtg cgatgacacc gtgctgctgt ttcagcagtg caccggctac gaccgggtga
     3361 tggtgtatcg tttcgatgag caaggccacg gcctggtatt ctccgagtgc catgtgcctg
     3421 ggctcgaatc ctatttcggc aaccgctatc cgtcgtcgct ggtcccgcag atggcgcggc
     3481 agctgtacgt gcggcagcgc gtccgcgtgc tggtcgacgt cacctatcag ccggtgccgc
     3541 tggagccgcg gctgtcgccg ctgaccgggc gcgatctcga catgtcgggc tgcttcctgc
     3601 gctcgatgtc gccgatccat ctgcagttcc tgaaggacat gggcgtgcgc gccaccctgg
     3661 cggtgtcgct ggtggtcggc ggcaagctgt ggggcctggt tgtctgtcac cattatctgc
     3721 cgcgcttcat ccgtttcgag ctgcgggcga tctgcaaacg gctcgccgaa aggatcgcga
     3781 cgcggatcac cgcgcttgag agcctgtaca agtctgggtc cgcagccagt agcgccggaa
     3841 gtatgatcag tctgattgcg gcgttagcgg tagatcgcgt tatcggcatg gaaaacgcca
     3901 tgccgtggaa cctgcctgcc gatctcgcct ggtttaaacg caacacctta aataaacccg
     3961 tgattatggg ccgccatacc tgggaatcaa tcggtcgtcc gttgccagga cgcaaaaata
     4021 ttatcctcag cagtcaaccg ggtacggaca aaaaaaagaa aaagaaagat cgcgtaacgt
     4081 gggtgaagtc ggtggatgaa gccatcgcgg cgtgtggtga cgtaccagaa atcatggtga
     4141 ttggcggcgg tcgcgtttat gaacagttct tgccaaaagc gcaaaaactg tatctgacgc
     4201 atatcgacgc agaagtggaa ggcgacaccc atttcccgga ttacgagccg gatgactggg
     4261 aatcggtatt cagcgaattc cacgatgctg atgcgcagaa ctctcacagc tattgctttg
     4321 agattctgga gcggcggagt gctggtggta gtgctggtgg tagtgctggt ggtagtgctg
     4381 gtggtagtgc tggtggtcct cgaagcctcg agatggagca catacaggga gcttggaaga
     4441 cgatcagcaa tggttttgga ttcaaagatg ccgtgtttga tggctccagc tgcatctctc
     4501 ctacaatagt tcagcagttt ggctatcagc gccgggcatc agatgatggc aaactcacag
     4561 atccttctaa gacaagcaac actatccgtg ttttcttgcc gaacaagcaa agaacagtgg
     4621 tcaatgtgcg aaatggaatg agcttgcatg actgccttat gaaagcactc aaggtgaggg
     4681 gcctgcaacc agagtgctgt gcagtgttca gacttctcca cgaacacaaa ggtaaaaaag
     4741 cacgcttaga ttggaatact gatgctgcgt ctttgattgg agaagaactt caagtagatt
     4801 tcctggatca tgttcccctc acaacacaca actttgctcg gaagacgttc ctgaagcttg
     4861 ccttctgtga catctgtcag aaattcctgc tcaatggatt tcgatgtcag acttgtggct
     4921 acaaatttca tgagcactgt agcaccaaag tacctactat gtgtgtggac tggagtaaca
     4981 tcagacaact cttattgttt ccaaattcca ctattggtga tagtggagtc ccagcactac
     5041 cttctttgac tatgcgtcgt atgcgagagt ctgtttccag gatgcctgtt agttctcagc
     5101 acagatattc tacacctcac gccttcacct ttaacacctc cagtccctca tctgaaggtt
     5161 ccctctccca gaggcagagg tcgacatcca cacctaatgt ccacatggtc agcaccaccc
     5221 tgcctgtgga cagcaggatg attgaggatg caattcgaag tcacagcgaa tcagcctcac
     5281 cttcagccct gtccagtagc cccaacaatc tgagcccaac aggctggtca cagccgaaaa
     5341 cccccgtgcc agcacaaaga gagcgggcac cagtatctgg gacccaggag aaaaacaaaa
     5401 ttaggcctcg tggacagaga gattcaagct attattggga aatagaagcc agtgaagtga
     5461 tgctgtccac tcggattggg tcaggctctt ttggaactgt ttataagggt aaatggcacg
     5521 gagatgttgc agtaaagatc ctaaaggttg tcgacccaac cccagagcaa ttccaggcct
     5581 tcaggaatga ggtggctgtt ctgcgcaaaa cacggcatgt gaacattctg cttttcatgg
     5641 ggtacatgac aaaggacaac ctggcaattg tgacccagtg gtgcgagggc agcagcctct
     5701 acaaacacct gcatgtccag gagaccaagt ttcagatgtt ccagctaatt gacattgccc
     5761 ggcagacggc tcagggaatg gactatttgc atgcaaagaa catcatccat agagacatga
     5821 aatccaacaa tatatttctc catgaaggct taacagtgaa aattggagat tttggtttgg
     5881 caacagtaaa gtcacgctgg agtggttctc agcaggttga acaacctact ggctctgtcc
     5941 tctggatggc cccagaggtg atccgaatgc aggataacaa cccattcagt ttccagtcgg
     6001 atgtctactc ctatggcatc gtattgtatg aactgatgac gggggagctt ccttattctc
     6061 acatcaacaa ccgagatcag atcatcttca tggtgggccg aggatatgcc tccccagatc
     6121 ttagtaagct atataagaac tgccccaaag caatgaagag gctggtagct gactgtgtga
     6181 agaaagtaaa ggaagagagg cctctttttc cccagatcct gtcttccatt gagctgctcc
     6241 aacactctct accgaagatc aaccggagcg cttccgagcc atccttgcat cgggcagccc
     6301 acactgagga tatcaatgct tgcacgctga ccacgtcccc gaggctgcct gtcttctgcg
     6361 gccgctagtc gacggtaccg cgggcccggg ataagtcaac taacttaagc tagcaacggt
     6421 ttccctctag cgggatcaat tccgcccccc ccccctaacg ttactggccg aagccgcttg
     6481 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     6541 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     6601 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     6661 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     6721 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     6781 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     6841 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     6901 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     6961 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gataatacca tggccaagtt
     7021 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
     7081 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
     7141 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
     7201 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
     7261 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
     7321 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
     7381 ctgaaagtcg agatgcatcg atgatctaga gctcgctgat cagcctcgac tgtgccttct
     7441 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     7501 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     7561 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     7621 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aacctgcagc
     7681 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     7741 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     7801 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     7861 tgccagcgga tcttaaaagt tttgttactt tatagaagaa attttgagtt tttgtttttt
     7921 tttaataaat aaataaacat aaataaattg tttgttgaat ttattattag tatgtaagtg
     7981 taaatataat aaaacttaat atctattcaa attaataaat aaacctcgat atacagaccg
     8041 ataaaacaca tgcgtcaatt ttacgcatga ttatctttaa cgtacgtcac aatatgatta
     8101 tctttctagg gttaatctag tatacgcgtt taattaatcc agcttttgtt ccctttagtg
     8161 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     8221 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8281 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8341 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8401 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8461 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8521 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8581 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8641 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8701 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8761 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8821 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8881 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8941 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9001 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     9061 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9121 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9181 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9241 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9301 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9361 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9421 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9481 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9541 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9601 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9661 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9721 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9781 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9841 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9901 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9961 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10021 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10081 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10141 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10201 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10261 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10321 atttccccga aaagtgccac
//
