LOCUS       sequence-419678-        9417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Dugbe virus GP protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9417)
  AUTHORS   Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F
  TITLE     Cell type-specific delivery by modular envelope design.
  JOURNAL   Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8.
  PUBMED    37612276
REFERENCE   2  (bases 1 to 9417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-23"; volume: "14(1):5141. doi"; pages: " 
            10.1038/s41467-023-40788-8"
COMMENT     Sequence Label: pHCMV_DUGV_GP
FEATURES             Location/Qualifiers
     source          1..9417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(6122..6141)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6187..6242
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6241..6260)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6600..6616)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6600..6616)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6613..6635)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6624..6640
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6648..6654,6655..6672,6673..6678))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6693..6714
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6831..6848)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7002..7590)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7082..7101)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7761..8552,8553..8621))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8384..8403
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8622..8726)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8752..9207)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8889..8910)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9101..9120
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9334..9356
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9348..9365
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9349..9365
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9375..9394
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9375..9393
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatgagc aaaagggtgc
     1441 tcattattgc cgtcgtcgtg tatctggtgt tcacaaccca gaaccagatc accggcaatc
     1501 acacaacaat taattctagc agccccagca caacagaagc cagctccacc cctacagtgt
     1561 ctagaacccc ccagaccacc accacatcca cagccgtgtc cacaacaatt accgctacaa
     1621 ccacccctac cgctagctgg accacacaga gccaatactt caacaaaaca acccagcacc
     1681 attggagaga agagaccatg atctctagaa atcccaccgt gctggacaga cagagcagag
     1741 ctagcagcgt gagagaactg ctgaacacca aattcctcat gctcctcggc tttatcccca
     1801 aaggcgaggt caaccacctc gaaaacgctt gcaatagaga gggaaaaaac tgtacagaac
     1861 tgattctgaa agaaaggatc gctagattct tcagcgaaac cgaaaaagaa agctgctaca
     1921 acacatatct ggaaaagcat ctgaggagcg tgagccccga agtgagcctc acaccttata
     1981 gagtgctggg actgagagag gacattctcc tcaaggagat tgacagaaga atcattagat
     2041 tcgaaaccga ctcccagaga gtcacatgcc tctccgcttc tctgctgaag cccgacgtgt
     2101 ttattagaga gcagagaatc gacgctaaac cctccaacgg acccaaaatc gtccccgtgg
     2161 actccgtggc ttgcatgaat ctcgaggcta acgtggacgt gaggtccaac aaactggtca
     2221 tccagagcct catgaccacc gtgaaaatta gcctcaagaa ctgcaaggtg gtggtgaata
     2281 gcagacagtg catccatcag caaaccggat ccggcgtgat taaggtgcct aaattcgaaa
     2341 agcagcaagg cggaacatgg tcctcctaca ttgctggcgt gtacacagcc accatcgatc
     2401 tgctggacga gaacaaccag aactgcaagc tgttcaccga gtgcatcgtg aagggcagag
     2461 aactggtgaa gggccagtcc gaactcaaga gcttcaacat tgaagtgctc ctccctaggg
     2521 tgatgaaaac cagaaggaag ctcctcgctg tcaccgacgg aagcacagag tgtaacagcg
     2581 gcacccaact catcgaagga aagtccatcg aagtgcataa gcaagacatc ggcggacccg
     2641 gcaagaaact gaccatctgc aacggcacct ccgtcctcga cgtgcctctc gatgaaggcc
     2701 acggatgtta caccatcaac gtgatcacat ccaagagagc ttgcagacct aagaactcca
     2761 agctccaatg ctccatcgac aaggagctca aaccttgcga tagcggcaaa tgcctctcca
     2821 tctcccaaaa gggagctggc cacatcaagg tgagcagagg caagaccatt ctgattacag
     2881 aatgtaaaga gcactgccaa atccccgtgc ccaccggaaa aggagatatt atggtggatt
     2941 gttccggcgg cagacagcac tacctcgagg tcaatatcgt ggacatccat tgccccaaca
     3001 ccaagtttct gggaggcatc atgctgtact tttgtaggat gagcagcaga cctaccgtcg
     3061 ctctgctgct gggcatttgg attggatgcg gctacatcct cacatgcatc ttcagctttc
     3121 tgctgtatca cctcattctg tttttcgcta actgtattaa gcaatgtaga aagaagggcg
     3181 agaggctggg cgaaatctgt gtcaagtgcg agcaacaaac cgtgaacctc atggatcaag
     3241 agctgcacga tctgaattgt aatttcaatc tgtgtcccta ctgttgcaat aggatgagcg
     3301 acgaaggaat gtctagacac gtgggcaagt gccccaaaag actcgaaaga ctgaacgaga
     3361 ttgaactcta tctgaccacc tccgagtgtc tctgcctcag cgtctgttac cagctgctga
     3421 ttagcgtcgg catttttctg aagagaacaa catggctcgt cgtgctcctc gtgctgctgg
     3481 gcctcgccat cagccccgtg caaggcgctc ctacagaggt cagcaatgtc aagcaagacg
     3541 gcgactacag catttgctac tttatcttcg gatgtctcgt gaccgctgct ctgctgctga
     3601 aggtcaagag gaccaacagc aatggaattg tggtggtcgt ggacagcttc ggcagatgcc
     3661 cttattgcaa cgagttcacc gactctctgt tcgaggaggt cctccacgac acactctgtt
     3721 ccctctgcgt gtgccccttt tgcgagaaac aagccctcga tctggtcaca ctggaagagc
     3781 acgtcaagga gtgctacaaa gtcgccacaa ggaaggacat ttttaagatt ctgggaagaa
     3841 agttcaccaa tgctctcgtg aggagggaaa aactgttcac caccggactg cagctcttta
     3901 tcaataaaac aaacgtggtc gtgttcgctc tgatcatgtg ctttctgctg ctgctgaccg
     3961 gccacaacgc tagcgccttt gattccggcg atctgcccga cggcgtgtgg gaagagtcct
     4021 cccagctggt caagagctgc acccagttct gctacatcga ggaggacgtc tgttattgcc
     4081 ccgccgagga cggcgtggga agaaagctgc tctttttcaa cggactgcaa aacagcgtca
     4141 agaggctcag cgattcccac aaactgctca caagcgtcag catcgacgcc ccttggggca
     4201 gaattaatgt ggagagcaca tggaagccca cactggccgc ttccaacatc gctatgtctt
     4261 ggagcagcac cgatattaaa ggagagaagg tgattctgtc cggaagatcc accagcatca
     4321 tcaaactcaa agaaaagacc ggcgtgatgt ggaaactcgt cggcagcggc ctcgcttccg
     4381 agaagaagaa acccttcaga ttccctatca tggacttcgc ccaagtgtac aacagcgtgt
     4441 tccaatacat caccggcgat agactgctca gcgagtggcc caaagccgtg tgcaccggcg
     4501 actgtcctca cagatgcggc tgtcagacaa gcacatgcat ggccaaagaa tgccacaccc
     4561 aagagtgcgt gagcacccat atggtgctgg gcatcggcac cggctgcaca tgctgtggaa
     4621 tggatgtgga aaggcccttc aacaagtatc tgggcgtcaa gtggtccaca gagtatctga
     4681 gaaccgaagt gctcgtgtgt gtggaagtga ccgaggagga gaggcactgt gagatcgtcg
     4741 aggctggaac aagatttaac atcggcccta tcaccatcac catcagcgac ccccagaata
     4801 ttggctccaa gctccccgaa agcctcatga cagtccaaga gatcgacgac tccaacttcg
     4861 tcgacatcat gcacgtggga aacgtgatct ccgccgacaa cagctgtaga ctgcagagct
     4921 gcacccatgg atccgctgtg acaaccagat ttacagctct gaccgctctg atcaaagacg
     4981 accactcctc cggactgaac ctcgccgtgc tggatcccaa agtcaatagc agctggctca
     5041 gctgggaggg ctgcgacatg gactactatt gtaatgtggg agactggccc acatgcacat
     5101 ataccggcgt cgtcacccaa aagctgagag agttcctcaa gctcgaccag catagaaaga
     5161 ggctgcacac caccctcagc ttttctctga agaagaatct gtccaaaagg tcccacacat
     5221 ccgtgagact cgagggcaaa acagtgacaa gaatggaggt gaaggtcaca gctctgattg
     5281 aagtggacgg catggagctc catagcaaga ccattagact cagcggaatc agactgaccg
     5341 gcctcaagtg cagcggctgt tttagctgca caagcggcat tagctgttcc gtgaacgcca
     5401 agctgacaag ccccgacgag tttacactgc acctcagatc cacatcccct aatgtggtcg
     5461 tggccgagac cagcattatc gccagaaaag gcccctccgc caccacaagc agattcaagg
     5521 tgttcagcgt gagggatacc aagaaaatct gcttcgaagt ggtggagagg gagtactgca
     5581 aagactgcac acccgacgaa ctgacaacat gcaccggcgt cgagctcgaa cctaccaagg
     5641 atattctgct ggaacatagg ggcaccatcg tccagcacca gaacgataca tgcaaatcca
     5701 aaatcgattg ttggagcaat tccatttcca gctttgcctc cggcatcggc gactttttta
     5761 agcattacat cggctccatc gctgtgggag tgctgggaac cgtgctcccc tttgccctcc
     5821 tcattctctt tttcatctat ggcgacaaga tgctctggcc cttcaaagtg ttctgtaggc
     5881 cttgtagaag atgctgtagg aagaacgaag gctacaacaa gctcgctgag gaggaggagc
     5941 tgagagatat cattaggaag tttagcaagt ccggagaact gattaacaag gacgccaagg
     6001 acaaaaggac actggctaga ctcttcatgt ccgataaccc taagctgaag aaggagaaaa
     6061 agctcagcga gatcgcctga tgtcttcctg gagctccagt cattgactat aacgtctcag
     6121 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt
     6181 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
     6241 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt
     6301 agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag
     6361 gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga
     6421 cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa
     6481 aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca
     6541 tagctgtccc tcttctctta tggagatccc tcgacggatc ggccgcaatt cgtaatcatg
     6601 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     6661 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     6721 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     6781 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     6841 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6901 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6961 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7021 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7081 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7141 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7201 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7261 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7321 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7381 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7441 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7501 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7561 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7621 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7681 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7741 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7801 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7861 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggca
     7921 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7981 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8041 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8101 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8161 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8221 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8281 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8341 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8401 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8461 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8521 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8581 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8641 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8701 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
     8761 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     8821 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     8881 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     8941 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     9001 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     9061 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     9121 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     9181 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
     9241 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     9301 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     9361 ccagtgagcg cgcgtaatac gactcactat agggcgaatt ggagctccac cgcggtg
//
