LOCUS       sequence-419682-       10216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Deliver gRNA through lentivirus (compatible with 10x capture 
            Sequence 1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10216)
  TITLE     LentiGuide-10X-CS1-3'-puro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LentiGuide-10X-CS1-3'-puro
FEATURES             Location/Qualifiers
     source          1..10216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        162..410
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     162..182
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     333..352
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    2456..2573
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2712..3890
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2943..3881
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3838..3858
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3903..4502
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3903..3922)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4399..4419
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4518..5106
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4571..4591)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4989..5000)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5178..5411
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5489..5610
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5526..5545)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5580..5599
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5650..5785
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5805..5824)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5806..5824)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5834..5851)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5834..5850)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5843..5865)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5992..6447
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6079..6098)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6289..6310
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6473..6577
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6578..6646,6647..7438)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6796..6815)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7609..8197
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8098..8117
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8351..8368
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8485..8506
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8521..8526,8527..8544,8545..8551)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8559..8575
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8564..8586
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8583..8599
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8583..8599
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8618..8638
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8620..8638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8666..8892
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8893..9073
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9120..9245
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9738..9971
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10156..10200
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
       61 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
      121 tatcgtttca gacccacctc ccaaccccga ggggacccag agagggccta tttcccatga
      181 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga
      241 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
      301 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
      361 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc ggagacggtt
      421 gtaaatgagc acacaaaata cacatgctaa aatattatat tctatgacct ttataaaatc
      481 aaccaaaatc ttctttttaa taactttagt atcaataatt agaattttta tgttcctttt
      541 tgcaaacttt taataaaaat gagcaaaata aaaaaacgct agttttagta actcgcgttg
      601 ttttcttcac ctttaataat agctactcca ccacttgttc ctaagcggtc agctcctgct
      661 tcaatcattt tttgagcatc ttcaaatgtt ctaactccac cagctgcttt aactaaagca
      721 ttgtctttaa caactgactt cattagttta acatcttcaa atgttgcacc tgattttgaa
      781 aatcctgttg atgttttaac aaattctaat ccagcttcaa cagctatttc acaagctttc
      841 atgatttctt cttttgttaa taaacaattt tccataatac atttaacaac atgtgatcca
      901 gctgcttttt ttacagcttt catgtcttct aaaactaatt cataattttt gtcttttaat
      961 gcaccaatat ttaataccat atcaatttct gttgcaccat ctttaattgc ttcagaaact
     1021 tcgaatgctt ttgtagctgt tgtgcatgca cctagaggaa aacctacaac atttgttatt
     1081 cctacatttg tgccttttaa taattcttta caatagcttg ttcaatatga attaacacaa
     1141 actgttgcaa aatcaaattc aattgcttca tcacataatt gtttaatttc agctttcgta
     1201 gcatcttgtt ttaataatgt gtgatctata tatttgttta gtttcatttt ttctcctata
     1261 tattcatttt taattttaat tctttaataa tttcgtctac tttaacttta gcgttttgaa
     1321 cagattcacc aacacctata aaataaattt ttagtttagg ttcagttcca cttgggcgaa
     1381 cagcaaatca tgacttatct tctaaataaa attttagtaa gtcttgtcct ggcatattat
     1441 acattccatc gatgtagtct tcaacattaa caactttaag tccagcaatt tgagttaagg
     1501 gtgttgctct caatgatttc attaatggtt caatttttaa tttcttttct tctggtttaa
     1561 aattcaagtt taaagtgaaa gtgtaatatg cacccatttc tttaaataaa tcttctaaat
     1621 agtctactaa tgttttattt tgttttttat aaaatcaagc agcctctgct attaatatag
     1681 aagcttgtat tccatcttta tctctagctg agtcatcaat tacatatcca taactttctt
     1741 cataagcaaa aacaaaattt aatccgttat cttcttcttt agcaatttct ctacccattc
     1801 atttaaatcc agttaaagtt tttacaatat taactccata tttttcatga gcgattctat
     1861 cacccaaatc acttgttaca aaacttgaat atagagccgg attttttgga atgctattta
     1921 agcgttttag atttgataat tttcaatcaa ttaaaattgg tcctgtttga tttccatcta
     1981 atcttacaaa atgaccatca tgttttattg ccattccaaa tctgtcagca tctgggtcat
     2041 tcataataat aatatctgca tcatgtttaa taccatattc aagcggtatt tttcatgcag
     2101 gatcaaattc tggatttgga tttacaacat ttttaaatgt ttcatcttca aatgcatgct
     2161 cttcaacctc aataacgtta tatcctgatt cacgtaatat ttttggggta aatttagttc
     2221 ctgttccatt aactgcgcta aaaataattt ttaaatcttt tttagcttct tgctcttttt
     2281 tgtacgtctc tgtttaagag ctaagctgga aacagcatag caagtttaaa taaggctagt
     2341 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcgct ttaaggccgg tcctagcaat
     2401 ttttttaagc ttggcgtaac tagatcttga gacaaatggc agtattcatc cacaatttta
     2461 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     2521 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     2581 attacaggga cagcagagat ccactttggc gccggctcga gggggcccgg gtgcaaagat
     2641 ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag
     2701 gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     2761 agaagttggg gggaggggtc ggcaattgat ccggtgccta gagaaggtgg cgcggggtaa
     2821 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     2881 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     2941 aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg
     3001 tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg
     3061 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag
     3121 ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct
     3181 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc
     3241 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg
     3301 tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc
     3361 ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg
     3421 ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc
     3481 ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct
     3541 caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa
     3601 gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca
     3661 ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt
     3721 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc
     3781 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca
     3841 agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga cgtacggcca
     3901 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     3961 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     4021 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     4081 acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg
     4141 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     4201 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     4261 agcccgcgtg gttcctggcc accgtcggag tctcgcccga ccaccagggc aagggtctgg
     4321 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     4381 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     4441 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     4501 gaacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4561 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4621 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     4681 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     4741 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     4801 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     4861 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     4921 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     4981 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5041 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     5101 ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta gccacttttt
     5161 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     5221 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     5281 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     5341 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     5401 aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc
     5461 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     5521 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     5581 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     5641 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     5701 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     5761 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     5821 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     5881 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     5941 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     6001 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6061 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6121 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     6181 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     6241 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     6301 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     6361 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     6421 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     6481 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     6541 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     6601 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     6661 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     6721 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     6781 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     6841 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     6901 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     6961 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     7021 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     7081 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     7141 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     7201 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     7261 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     7321 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     7381 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     7441 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     7501 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7561 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7621 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7681 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7741 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7801 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7861 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7921 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7981 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8041 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8101 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8161 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8221 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     8281 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     8341 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     8401 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     8461 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     8521 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     8581 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     8641 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
     8701 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
     8761 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
     8821 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
     8881 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8941 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     9001 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     9061 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     9121 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     9181 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     9241 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     9301 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     9361 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     9421 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     9481 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     9541 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     9601 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     9661 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     9721 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     9781 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     9841 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     9901 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     9961 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
    10021 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
    10081 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
    10141 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
    10201 tggaattggt ttaaca
//
