LOCUS       sequence-41973-e        1237 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Dillin A, Crawford DK, Kenyon C
  TITLE     Timing requirements for insulin/IGF-1 signaling in C. elegans.
  JOURNAL   Science. 2002 Oct 25;298(5594):830-4.
  PUBMED    12399591
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2002-10-25"; volume: "298(5594)"; pages: "830-4"
COMMENT     Sequence Label: pAD12
FEATURES             Location/Qualifiers
     source          1..1237
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..53
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     38..54
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(106..125)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(107..125)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(147..167)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(147..165)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(186..202)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(186..202)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(199..221)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    210..226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(234..240,241..258,259..264))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    279..300
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(417..434)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(679..698)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcacgaatc tatgggcgat tgggtccggg cccccctcga ggtcgacggt atcgataagc
       61 ttgatatcga attcctgcag cccgggggat ccactagtgc ttcgccccta tagtgagtcg
      121 tattacgcga gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
      181 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      241 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      301 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      361 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
      421 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      481 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      541 ggccagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      601 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      661 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      721 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      781 tggcgctttc tcatagctca cgctgtaggt atctcagatc ggtgtaggtc gttcgctcca
      841 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      901 atcgtcttga gttcacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
      961 caggattatc atagcgagtt atgtaagcng tgctacaaag ttcttgaagt ggtggtctaa
     1021 ctacggctac cttagaagaa cacaattggg tatctgccct ctgctgaagc cagttacctt
     1081 ccggaaaaga gttggtaggc tctgatccgg caaaacaaac caccctggta acggggggtt
     1141 ttttttgttg gcaagcacca aatttccccc cgaaaaaaag gattttccaa aagatccttt
     1201 gaattttttt ctacggggct tgaaaccctc tggggaa
//
