LOCUS       sequence-419733-        9992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A vector backbone domesticated for SapI (pCC1BAC-based), contains 
            elements for selection, maintenance, and propagation in S. 
            cerevisiae (TRP1) and E. coli (cat)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9992)
  AUTHORS   Walker EJL, Pampuch M, Chang N, Cochrane RR, Karas BJ
  TITLE     Design and assembly of the 117-kb Phaeodactylum tricornutum 
            chloroplast genome.
  JOURNAL   Plant Physiol. 2023 Dec 19:kiad670. doi: 10.1093/plphys/kiad670.
  PUBMED    38114089
REFERENCE   2  (bases 1 to 9992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 Dec 19:kiad670. doi: 10.1093/plphys/kiad670."
COMMENT     Sequence Label: pSAP
FEATURES             Location/Qualifiers
     source          1..9992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..615
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      92..623
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      691..910
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             1001..1756
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    1759..2009
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     misc_feature    2171..2674
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        2728..2875
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             2876..3550
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     CDS             3946..5121
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             5121..6092
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    7156..7554
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(7572..7605)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     7673..7692
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(7772..8047)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGETIEYSSLHACRSTLEDPRVPSSNSPYSESYYNSL
                     AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS"
     primer_bind     7885..7907
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7899..7916
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7900..7916
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7923..7942
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7923..7941
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7944..8000
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8043..8059)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8043..8059)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8056..8078)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8067..8083
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8091..8097,8098..8115,8116..8121))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8136..8157
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(8377..9036)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     8951..8970
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(9037..9139)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ctggttgccc tcgccgctgg gctggcggcc gtctatggcc ctgcaaacgc gccagaaacg
       61 ccgtcgaagc cgtgtgcgag acaccgcggc cggccgccgg cgttgtggat acctcgcgga
      121 aaacttggcc ctcactgaca gatgaggggc ggacgttgac acttgagggg ccgactcacc
      181 cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc
      241 agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga tgatgtggac
      301 aagcctgggg ataagtgccc tgcggtattg acacttgagg ggcgcgacta ctgacagatg
      361 aggggcgcga tccttgacac ttgaggggca gagtgctgac agatgagggg cgcacctatt
      421 gacatttgag gggctgtcca caggcagaaa atccagcatt tgcaagggtt tccgcccgtt
      481 tttcggccac cgctaacctg tcttttaacc tgcttttaaa ccaatattta taaaccttgt
      541 ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc
      601 ttctcgaacc ctcccggtcg agtgagcgag gaagcaccag ggaacagcac ttatatattc
      661 tgcttacaca cgatgcctga aaaaacttcc cttggggtta tccacttatc cacggggata
      721 tttttataat tatttttttt atagttttta gatcttcttt tttagagcgc cttgtaggcc
      781 tttatccatg ctggttctag agaaggtgtt gtgacaaatt gccctttcag tgtgacaaat
      841 caccctcaaa tgacagtcct gtctgtgaca aattgccctt aaccctgtga caaattgccc
      901 tcagaagaag ctgttttttc acaaagttat ccctgcttat tgactctttt ttatttagtg
      961 tgacaatcta aaaacttgtc acacttcaca tggatctgtc atggcggaaa cagcggttat
     1021 caatcacaag aaacgtaaaa atagcccgcg aatcgtccag tcaaacgacc tcactgaggc
     1081 ggcatatagt ctctcccggg atcaaaaacg tatgctgtat ctgttcgttg accagatcag
     1141 aaaatctgat ggcaccctac aggaacatga cggtatctgc gagatccatg ttgctaaata
     1201 tgctgaaata ttcggattga cctctgcgga agccagtaag gatatacggc aggcattgaa
     1261 gagtttcgcg gggaaggaag tggtttttta tcgccctgaa gaggatgccg gcgatgaaaa
     1321 aggctatgaa tcttttcctt ggtttatcaa acgtgcgcac agtccatcca gagggcttta
     1381 cagtgtacat atcaacccat atctcattcc cttctttatc gggttacaga accggtttac
     1441 gcagtttcgg cttagtgaaa caaaagaaat caccaatccg tatgccatgc gtttatacga
     1501 atccctgtgt cagtatcgta agccggatgg ctcaggcatc gtctctctga aaatcgactg
     1561 gatcatagag cgttaccagc tgcctcaaag ttaccagcgt atgcctgact tccgccgccg
     1621 cttcctgcag gtctgtgtta atgagatcaa cagcagaact ccaatgcgcc tctcatacat
     1681 tgagaaaaag aaaggccgcc agacgactca tatcgtattt tccttccgcg atatcacttc
     1741 catgacgaca ggatagtctg agggttatct gtcacagatt tgagggtggt tcgtcacatt
     1801 tgttctgacc tactgagggt aatttgtcac agttttgctg tttccttcag cctgcatgga
     1861 ttttctcata ctttttgaac tgtaattttt aaggaagcca aatttgaggg cagtttgtca
     1921 cagttgattt ccttctcttt cccttcgtca tgtgacctga tatcgggggt tagttcgtca
     1981 tcattgatga gggttgatta tcacagttta ttactctgaa ttggctatcc gcgtgtgtac
     2041 ctctacctgg agtttttccc acggtggata tttcttcttg cgctgagcgt aagagctatc
     2101 tgacagaaca gttcttcttt gcttcctcgc cagttcgctc gctatgctcg gttacacggc
     2161 tgcggcgagc atcacgtgct ataaaaataa ttataattta aattttttaa tataaatata
     2221 taaattaaaa atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa
     2281 gatgtaaaag actctagggg gatcgccaac aaatactacc ttttaccttg ctctacctgc
     2341 tctcaggtat taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat
     2401 cctgtgattt tacattttac ttatcgttaa tcgaatgtat atctatttaa tctgcttttc
     2461 ttgtctaata aatatatatg taaagtacgc tttttgttga aattttttaa acctttgttt
     2521 attttttttt cttcattccg taactcttct accttcttta tttactttct aaaatccaaa
     2581 tacaaaacat aaaaataaat aaacacagag taaattccca aattattcca tcattaaaag
     2641 atacgaggcg cgtgtaagtt acaggcaagc gatccagatg gcagtagtgg aagatattct
     2701 ttattgaaaa atagcttgtc accttacgta caatcttgat ccggagcttt tctttttttg
     2761 ccgattaaga attaattcgg tcgaaaaaag aaaaggagag ggccaagagg gagggcattg
     2821 gtgactattg agcacgtgag tatacgtgat taagcacaca aaggcagctt ggagtatgtc
     2881 tgttattaat ttcacaggta gttctggtcc attggtgaaa gtttgcggct tgcagagcac
     2941 agaggccgca gaatgtgctc tagattccga tgctgacttg ctgggtatta tatgtgtgcc
     3001 caatagaaag agaacaattg acccggttat tgcaaggaaa atttcaagtc ttgtaaaagc
     3061 atataaaaat agttcaggca ctccgaaata cttggttggc gtgtttcgta atcaacctaa
     3121 ggaggatgtt ttggctctgg tcaatgatta cggcattgat atcgtccaac tgcatggaga
     3181 tgagtcgtgg caagaatacc aagagttcct cggtttgcca gttattaaaa gactcgtatt
     3241 tccaaaagac tgcaacatac tactcagtgc agcttcacag aaacctcatt cgtttattcc
     3301 cttgtttgat tcagaagcag gtgggacagg tgaacttttg gattggaact cgatttctga
     3361 ctgggttgga aggcaagaga gccccgaaag cttacatttt atgttagctg gtggactgac
     3421 gccagaaaat gttggtgatg cgcttagatt aaatggcgtt attggtgttg atgtaagcgg
     3481 aggtgtggag acaaatggtg taaaagactc taacaaaata gcaaatttcg tcaaaaatgc
     3541 taagaaatag gttattactg agtagtattt atttaagtat tgtttgtgca cttgccgatc
     3601 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta
     3661 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     3721 caataggccg aaatcggcaa aatcccttat aaatcatttt acacaggagt ctggacttga
     3781 cgctagtgat aataagtgac tgaggtatgt gcgcttctta tctccttttg tagtgttgct
     3841 cttattttaa acaactttgc ggttttttga tgactttgcg attttgttgt tgctttgcag
     3901 taaattgcaa gatttaataa aaaaacgcaa agcaatgatt aaaggatgtt cagaatgaaa
     3961 ctcatggaaa cacttaacca gtgcataaac gctggtcatg aaatgacgaa ggctatcgcc
     4021 attgcacagt ttaatgatga cagcccggaa gcgaggaaaa taacccggcg ctggagaata
     4081 ggtgaagcag cggatttagt tggggtttct tctcaggcta tcagagatgc cgagaaagca
     4141 gggcgactac cgcacccgga tatggaaatt cgaggacggg ttgagcaacg tgttggttat
     4201 acaattgaac aaattaatca tatgcgtgat gtgtttggta cgcgattgcg acgtgctgaa
     4261 gacgtatttc caccggtgat cggggttgct gcccataaag gtggcgttta caaaacctca
     4321 gtttctgttc atcttgctca ggatctggct ctgaaggggc tacgtgtttt gctcgtggaa
     4381 ggtaacgacc cccagggaac agcctcaatg tatcacggat gggtaccaga tcttcatatt
     4441 catgcagaag acactctcct gcctttctat cttggggaaa aggacgatgt cacttatgca
     4501 ataaagccca cttgctggcc ggggcttgac attattcctt cctgtctggc tctgcaccgt
     4561 attgaaactg agttaatggg caaatttgat gaaggtaaac tgcccaccga tccacacctg
     4621 atgctccgac tggccattga aactgttgct catgactatg atgtcatagt tattgacagc
     4681 gcgcctaacc tgggtatcgg cacgattaat gtcgtatgtg ctgctgatgt gctgattgtt
     4741 cccacgcctg ctgagttgtt tgactacacc tccgcactgc agtttttcga tatgcttcgt
     4801 gatctgctca agaacgttga tcttaaaggg ttcgagcctg atgtacgtat tttgcttacc
     4861 aaatacagca atagcaatgg ctctcagtcc ccgtggatgg aggagcaaat tcgggatgcc
     4921 tggggaagca tggttctaaa aaatgttgta cgtgaaacgg atgaagttgg taaaggtcag
     4981 atccggatga gaactgtttt tgaacaggcc attgatcaac gctcatcaac tggtgcctgg
     5041 agaaatgctc tttctatttg ggaacctgtc tgcaatgaaa ttttcgatcg tctgattaaa
     5101 ccacgctggg agattagata atgaagcgtg cgcctgttat tccaaaacat acgctcaata
     5161 ctcaaccggt tgaagatact tcgttatcga caccagctgc cccgatggtg gattcgttaa
     5221 ttgcgcgcgt aggagtaatg gctcgcggta atgccattac tttgcctgta tgtggtcggg
     5281 atgtgaagtt tactcttgaa gtgctccggg gtgatagtgt tgagaagacc tctcgggtat
     5341 ggtcaggtaa tgaacgtgac caggagctgc ttactgagga cgcactggat gatctcatcc
     5401 cttcttttct actgactggt caacagacac cggcgttcgg tcgaagagta tctggtgtca
     5461 tagaaattgc cgatgggagt cgccgtcgta aagctgctgc acttaccgaa agtgattatc
     5521 gtgttctggt tggcgagctg gatgatgagc agatggctgc attatccaga ttgggtaacg
     5581 attatcgccc aacaagtgct tatgaacgtg gtcagcgtta tgcaagccga ttgcagaatg
     5641 aatttgctgg aaatatttct gcgctggctg atgcggaaaa tatttcacgt aagattatta
     5701 cccgctgtat caacaccgcc aaattgccta aatcagttgt tgctcttttt tctcaccccg
     5761 gtgaactatc tgcccggtca ggtgatgcac ttcaaaaagc ctttacagat aaagaggaat
     5821 tacttaagca gcaggcatct aaccttcatg agcagaaaaa agctggggtg atatttgaag
     5881 ctgaagaagt tatcactctt ttaacttctg tgcttaaaac gtcatctgca tcaagaacta
     5941 gtttaagctc acgacatcag tttgctcctg gagcgacagt attgtataag ggcgataaaa
     6001 tggtgcttaa cctggacagg tctcgtgttc caactgagtg tatagagaaa attgaggcca
     6061 ttcttaagga acttgaaaag ccagcaccct gatgcgacct cgttttagtc tacgtttatc
     6121 tgtctttact taatgtcctt tgttacaggc cagaaagcat aactggcctg aatattctct
     6181 ctgggcccac tgttccactt gtatcgtcgg tctgataatc agactgggac cacggtccca
     6241 ctcgtatcgt cggtctgatt attagtctgg gaccacggtc ccactcgtat cgtcggtctg
     6301 attattagtc tgggaccacg gtcccactcg tatcgtcggt ctgataatca gactgggacc
     6361 acggtcccac tcgtatcgtc ggtctgatta ttagtctggg accatggtcc cactcgtatc
     6421 gtcggtctga ttattagtct gggaccacgg tcccactcgt atcgtcggtc tgattattag
     6481 tctggaacca cggtcccact cgtatcgtcg gtctgattat tagtctggga ccacggtccc
     6541 actcgtatcg tcggtctgat tattagtctg ggaccacggt cccactcgta tcgtcggtct
     6601 gataatcaga ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac
     6661 catggtccca ctcgtatcgt cggtctgatt attagtctgg gaccacggtc ccactcgtat
     6721 cgtcggtctg attattagtc tggaaccacg gtcccactcg tatcgtcggt ctgattatta
     6781 gtctgggacc acggtcccac tcgtatcgtc ggtctgatta ttagtctggg accacgatcc
     6841 cactcgtgtt gtcggtctga ttatcggtct gggaccacgg tcccacttgt attgtcgatc
     6901 agactatcag cgtgagacta cgattccatc aatgcctgtc aagggcaagt attgacatgt
     6961 cgtcgtaacc tgtagaacgg agtaacctcg gtgtgcggtt gtatgcctgc tgtggattgc
     7021 tgctgtgtcc tgcttatcca caacattttg cgcacggtta tgtggacaaa atacctggtt
     7081 acccaggccg tgccggcacg ttaaccgggc tgcatccgat gcaagtgtgt cgctgtcgac
     7141 gagctcgcga gctcggacat gaggttgccc cgtattcagt gtcgctgatt tgtattgtct
     7201 gaagttgttt ttacgttaag ttgatgcaga tcaattaata cgatacctgc gtcataattg
     7261 attatttgac gtggtttgat ggcctccacg cacgttgtga tatgtagatg ataatcatta
     7321 tcactttacg ggtcctttcc ggtgatccga caggttacgg ggcggcgacc tcgcgggttt
     7381 tcgctattta tgaaaatttt ccggtttaag gcgtttccgt tcttcttcgt cataacttaa
     7441 tgtttttatt taaaataccc tctgaaaaga aaggaaacga caggtgctga aagcgagctt
     7501 tttggcctct gtcgtttcct ttctctgttt ttgtccgtgg aatgaacaat ggaagtccga
     7561 gctcatcgct aataacttcg tatagcatac attatacgaa gttatattcg atgcggccgc
     7621 aaggggttcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     7681 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccacacag atgcgtaagg
     7741 agaaaatacc gcatcaggcg ccattcgcca ttcagctgcg caactgttgg gaagggcgat
     7801 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     7861 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
     7921 tgtaatacga ctcactatag ggcgaattcg agctcggtac ccggggatcc tctagagtcg
     7981 acctgcaggc atgcaagctt gagtattcta tagtctcacc taaatagctt ggcgtaatca
     8041 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8101 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     8161 gcgttgcgct cactgcccgc tttctagtcg ggaaacctgt cgtgccagct gcattaatga
     8221 atcggccaac gcgaacccct tgcggccgcc cgggccgtcg accaattctc atgtttgaca
     8281 gcttatcatc gaatttctgc cattcatccg cttattatca cttattcagg cgtagcaacc
     8341 aggcgtttaa gggcaccaat aactgcctta aaaaaattac gccccgccct gccactcatc
     8401 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     8461 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     8521 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     8581 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata
     8641 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     8701 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     8761 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
     8821 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     8881 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     8941 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     9001 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     9061 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     9121 acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt
     9181 atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta
     9241 ttcgcgataa gctcatggag cggcgtaacc gtcgcacagg aaggacagag aaagcgcgga
     9301 tctgggaagt gacggacaga acggtcagga cctggattgg ggaggcggtt gccgccgctg
     9361 ctgctgacgg tgtgacgttc tctgttccgg tcacaccaca tacgttccgc cattcctatg
     9421 cgatgcacat gctgtatgcc ggtataccgc tgaaagttct gcaaagcctg atgggacata
     9481 agtccatcag ttcaacggaa gtctacacga aggtttttgc gctggatgtg gctgcccggc
     9541 accgggtgca gtttgcgatg ccggagtctg atgcggttgc gatgctgaaa caattatcct
     9601 gagaataaat gccttggcct ttatatggaa atgtggaact gagtggatat gctgtttttg
     9661 tctgttaaac agagaagctg gctgttatcc actgagaagc gaacgaaaca gtcgggaaaa
     9721 tctcccatta tcgtagagat ccgcattatt aatctcagga gcctgtgtag cgtttatagg
     9781 aagtagtgtt ctgtcatgat gcctgcaagc ggtaacgaaa acgatttgaa tatgccttca
     9841 ggaacaatag aaatcttcgt gcggtgttac gttgaagtgg agcggattat gtcagcaatg
     9901 gacagaacaa cctaatgaac acagaaccat gatgtggtct gtccttttac agccagtagt
     9961 gctcgccgca gtcgagcgac agggcgaagc cc
//
