LOCUS       sequence-419745-       14331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A vector backbone (pCC1BAC-based) containing a single SapI 
            recognition site. When digested, it exposes overhangs that allow 
            integration of the vector into the P. tricornutum chloroplast 
            genome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14331)
  AUTHORS   Walker EJL, Pampuch M, Chang N, Cochrane RR, Karas BJ
  TITLE     Design and assembly of the 117-kb Phaeodactylum tricornutum 
            chloroplast genome.
  JOURNAL   Plant Physiol. 2023 Dec 19:kiad670. doi: 10.1093/plphys/kiad670.
  PUBMED    38114089
REFERENCE   2  (bases 1 to 14331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 Dec 19:kiad670. doi: 10.1093/plphys/kiad670."
COMMENT     Sequence Label: pINTO_7/8
FEATURES             Location/Qualifiers
     source          1..14331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..756
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    759..1009
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     misc_feature    1171..1674
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        1675..1861
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             1862..2524
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     CDS             2714..3889
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             3889..4860
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    4933..5406
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    5666..6064
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(6082..6115)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     6183..6202
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(6282..6557)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGETIEYSSLHACRSTLEDPRVPSSNSPYSESYYNSL
                     AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS"
     primer_bind     6395..6417
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6409..6426
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6410..6426
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6433..6452
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6433..6451
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    6454..6510
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(6553..6569)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6553..6569)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6566..6588)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6577..6593
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6601..6607,6608..6625,6626..6631))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6646..6667
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(6887..7546)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7461..7480
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(7547..7649)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             9102..9671
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGFT
                     LREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNRR
                     LTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFTL
                     CGLDTALYDGTASDGEQALYMSMPCP"
     oriT            10147..10256
                     /note="incP origin of transfer"
     CDS             10289..10660
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERKAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      13332..13946
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      13423..13954
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      14022..14241
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
ORIGIN
        1 atggcggaaa cagcggttat caatcacaag aaacgtaaaa atagcccgcg aatcgtccag
       61 tcaaacgacc tcactgaggc ggcatatagt ctctcccggg atcaaaaacg tatgctgtat
      121 ctgttcgttg accagatcag aaaatctgat ggcaccctac aggaacatga cggtatctgc
      181 gagatccatg ttgctaaata tgctgaaata ttcggattga cctctgcgga agccagtaag
      241 gatatacggc aggcattgaa gagtttcgcg gggaaggaag tggtttttta tcgccctgaa
      301 gaggatgccg gcgatgaaaa aggctatgaa tcttttcctt ggtttatcaa acgtgcgcac
      361 agtccatcca gagggcttta cagtgtacat atcaacccat atctcattcc cttctttatc
      421 gggttacaga accggtttac gcagtttcgg cttagtgaaa caaaagaaat caccaatccg
      481 tatgccatgc gtttatacga atccctgtgt cagtatcgta agccggatgg ctcaggcatc
      541 gtctctctga aaatcgactg gatcatagag cgttaccagc tgcctcaaag ttaccagcgt
      601 atgcctgact tccgccgccg cttcctgcag gtctgtgtta atgagatcaa cagcagaact
      661 ccaatgcgcc tctcatacat tgagaaaaag aaaggccgcc agacgactca tatcgtattt
      721 tccttccgcg atatcacttc catgacgaca ggatagtctg agggttatct gtcacagatt
      781 tgagggtggt tcgtcacatt tgttctgacc tactgagggt aatttgtcac agttttgctg
      841 tttccttcag cctgcatgga ttttctcata ctttttgaac tgtaattttt aaggaagcca
      901 aatttgaggg cagtttgtca cagttgattt ccttctcttt cccttcgtca tgtgacctga
      961 tatcgggggt tagttcgtca tcattgatga gggttgatta tcacagttta ttactctgaa
     1021 ttggctatcc gcgtgtgtac ctctacctgg agtttttccc acggtggata tttcttcttg
     1081 cgctgagcgt aagagctatc tgacagaaca gttcttcttt gcttcctcgc cagttcgctc
     1141 gctatgctcg gttacacggc tgcggcgagc atcacgtgct ataaaaataa ttataattta
     1201 aattttttaa tataaatata taaattaaaa atagaaagta aaaaaagaaa ttaaagaaaa
     1261 aatagttttt gttttccgaa gatgtaaaag actctagggg gatcgccaac aaatactacc
     1321 ttttaccttg ctcatcctgc tctcaggtat taatgccgaa ttgtttcatc ttgtctgtgt
     1381 agaagaccac acacgaaaat cctgtgattt tacattttac ttatcgttaa tcgaatgtat
     1441 atctatttaa tctgcttttc ttgtctaata aatatatatg taaagtacgc tttttgttga
     1501 aattttttaa acctttgttt attttttttt cttcattccg taactcttct accttcttta
     1561 tttactttct aaaatccaaa tacaaaacat aaaaataaat aaacacagag taaattccca
     1621 aattattcca tcattaaaag atacgaggcg cgtgtaagtt acaggcaagc gatcctagta
     1681 cactctatat ttttttatgc ctcggtaatg attttcattt tttttttcca cctagcggat
     1741 gactcttttt ttttcttagc gattggcatt atcacataat gaattataca ttatataaag
     1801 taatgtgatt tcttcgaaga atatactaaa aaatgagcag gcaagataaa cgaaggcaaa
     1861 gatgacagag cagaaagccc tagtaaagcg tattacaaat gaaaccaaga ttcagattgc
     1921 gatctcttta aagggtggtc ccctagcgat agagcactcg atcttcccag aaaaagaggc
     1981 agaagcagta gcagaacagg ccacacaatc gcaagtgatt aacgtccaca caggtatagg
     2041 gtttctggac catatgatac atgctctggc caagcattcc ggctggtcgc taatcgttga
     2101 gtgcattggt gacttacaca tagacgacca tcacaccact gaagactgcg ggattgctct
     2161 cggtcaagct tttaaagagg ccctaggggc cgtgcgtgga gtaaaaaggt ttggatcagg
     2221 atttgcgcct ttggatgagg cactttccag agcggtggta gatctttcga acaggccgta
     2281 cgcagttgtc gaacttggtt tgcaaaggga gaaagtagga gatctctctt gcgagatgat
     2341 cccgcatttt cttgaaagct ttgcagaggc tagcagaatt accctccacg ttgattgtct
     2401 gcgaggcaag aatgatcatc accgtagtga gagtgcgttc aaggctcttg cggttgccat
     2461 aagagaagcc acctcgccca atggtaccaa cgatgttccc tccaccaaag gtgttcttat
     2521 gtagttttac acaggagtct ggacttgacg ctagtgataa taagtgactg aggtatgtgc
     2581 tcatcttatc tccttttgta gtgttgctct tattttaaac aactttgcgg ttttttgatg
     2641 actttgcgat tttgttgttg ctttgcagta aattgcaaga tttaataaaa aaacgcaaag
     2701 caatgattaa aggatgttca gaatgaaact catggaaaca cttaaccagt gcataaacgc
     2761 tggtcatgaa atgacgaagg ctatcgccat tgcacagttt aatgatgaca gcccggaagc
     2821 gaggaaaata acccggcgct ggagaatagg tgaagcagcg gatttagttg gggtttcttc
     2881 tcaggctatc agagatgccg agaaagcagg gcgactaccg cacccggata tggaaattcg
     2941 aggacgggtt gagcaacgtg ttggttatac aattgaacaa attaatcata tgcgtgatgt
     3001 gtttggtacg cgattgcgac gtgctgaaga cgtatttcca ccggtgatcg gggttgctgc
     3061 ccataaaggt ggcgtttaca aaacctcagt ttctgttcat cttgctcagg atctggctct
     3121 gaaggggcta cgtgttttgc tcgtggaagg taacgacccc cagggaacag cctcaatgta
     3181 tcacggatgg gtaccagatc ttcatattca tgcagaagac actctcctgc ctttctatct
     3241 tggggaaaag gacgatgtca cttatgcaat aaagcccact tgctggccgg ggcttgacat
     3301 tattccttcc tgtctggctc tgcaccgtat tgaaactgag ttaatgggca aatttgatga
     3361 aggtaaactg cccaccgatc cacacctgat gctccgactg gccattgaaa ctgttgctca
     3421 tgactatgat gtcatagtta ttgacagcgc gcctaacctg ggtatcggca cgattaatgt
     3481 cgtatgtgct gctgatgtgc tgattgttcc cacgcctgct gagttgtttg actacacctc
     3541 cgcactgcag tttttcgata tgcttcgtga tctgctcaag aacgttgatc ttaaagggtt
     3601 cgagcctgat gtacgtattt tgcttaccaa atacagcaat agcaatggct ctcagtcccc
     3661 gtggatggag gagcaaattc gggatgcctg gggaagcatg gttctaaaaa atgttgtacg
     3721 tgaaacggat gaagttggta aaggtcagat ccggatgaga actgtttttg aacaggccat
     3781 tgatcaacgt tcttcaactg gtgcctggag aaatgctctt tctatttggg aacctgtctg
     3841 caatgaaatt ttcgatcgtc tgattaaacc acgctgggag attagataat gaagcgtgcg
     3901 cctgttattc caaaacatac gctcaatact caaccggttg aagatacttc gttatcgaca
     3961 ccagctgccc cgatggtgga ttcgttaatt gcgcgcgtag gagtaatggc tcgcggtaat
     4021 gccattactt tgcctgtatg tggtcgggat gtgaagttta ctcttgaagt gctccggggt
     4081 gatagtgttg agaagacctc tcgggtatgg tcaggtaatg aacgtgacca ggagctgctt
     4141 actgaggacg cactggatga tctcatccct tcttttctac tgactggtca acagacaccg
     4201 gcgttcggtc gaagagtatc tggtgtcata gaaattgccg atgggagtcg ccgtcgtaaa
     4261 gctgctgcac ttaccgaaag tgattatcgt gttctggttg gcgagctgga tgatgagcag
     4321 atggctgcat tatccagatt gggtaacgat tatcgcccaa caagtgctta tgaacgtggt
     4381 cagcgttatg caagccgatt gcagaatgaa tttgctggaa atatttctgc gctggctgat
     4441 gcggaaaata tttcacgtaa gattattacc cgctgtatca acaccgccaa attgcctaaa
     4501 tcagttgttg ctcttttttc tcaccccggt gaactatctg cccggtcagg tgatgcactt
     4561 caaaaagcct ttacagataa agaggaatta cttaagcagc aggcatctaa ccttcatgag
     4621 cagaaaaaag ctggggtgat atttgaagct gaagaagtta tcactctttt aacttctgtg
     4681 cttaaaacgt catctgcatc aagaactagt ttaagctcac gacatcagtt tgctcctgga
     4741 gcgacagtat tgtataaggg cgataaaatg gtgcttaacc tggacaggtc tcgtgttcca
     4801 actgagtgta tagagaaaat tgaggccatt cttaaggaac ttgaaaagcc agcaccctga
     4861 tgcgacctcg ttttagtcta cgtttatctg tctttactta atgtcctttg ttacaggcca
     4921 gaaagcataa ctggcctgaa tattctctct gggcccactg ttccacttgt atcgtcggtc
     4981 tgataatcag actgggacca cggtcccact cgtatcgtcg gtctgattat tagtctggga
     5041 ccacggtccc actcgtatcg tcggtctgat tattagtctg ggaccacggt cccactcgta
     5101 tcgtcggtct gataatcaga ctgggaccac ggtcccactc gtatcgtcgg tctgattatt
     5161 agtctgggac catggtccca ctcgtatcgt cggtctgatt attagtctgg gaccacggtc
     5221 ccactcgtat cgtcggtctg attattagtc tggaaccacg gtcccactcg tatcgtcggt
     5281 ctgattatta gtctgggacc acggtcccac tcgtatcgtc ggtctgatta ttagtctggg
     5341 accacgatcc cactcgtgtt gtcggtctga ttatcggtct gggaccacgg tcccacttgt
     5401 attgtcgatc agactatcag cgtgagacta cgattccatc aatgcctgtc aagggcaagt
     5461 attgacatgt cgtcgtaacc tgtagaacgg agtaacctcg gtgtgcggtt gtatgcctgc
     5521 tgtggattgc tgctgtgtcc tgcttatcca caacattttg cgcacggtta tgtggacaaa
     5581 atacctggtt acccaggccg tgccggcacg ttaaccgggc tgcatccgat gcaagtgtgt
     5641 cgctgtcgac gagctcgcga gctcggacat gaggttgccc cgtattcagt gtcgctgatt
     5701 tgtattgtct gaagttgttt ttacgttaag ttgatgcaga tcaattaata cgatacctgc
     5761 gtcataattg attatttgac gtggtttgat ggcctccacg cacgttgtga tatgtagatg
     5821 ataatcatta tcactttacg ggtcctttcc ggtgatccga caggttacgg ggcggcgacc
     5881 tcgcgggttt tcgctattta tgaaaatttt ccggtttaag gcgtttccgt tcttcttcgt
     5941 cataacttaa tgtttttatt taaaataccc tctgaaaaga aaggaaacga caggtgctga
     6001 aagcgagctt tttggcctct gtcgtttcct ttctctgttt ttgtccgtgg aatgaacaat
     6061 ggaagtccga gctcatcgct aataacttcg tatagcatac attatacgaa gttatattcg
     6121 atgcggccgc aaggggttcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     6181 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccacacag
     6241 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcagctgcg caactgttgg
     6301 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     6361 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     6421 gccagtgaat tgtaatacga ctcactatag ggcgaattcg agctcggtac ccggggatcc
     6481 tctagagtcg acctgcaggc atgcaagctt gagtattcta tagtctcacc taaatagctt
     6541 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     6601 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6661 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     6721 gcattaatga atcggccaac gcgaacccct tgcggccgcc cgggccgtcg accaattctc
     6781 atgtttgaca gcttatcatc gaatttctgc cattcatccg cttattatca cttattcagg
     6841 cgtagcaacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac gccccgccct
     6901 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     6961 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     7021 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     7081 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     7141 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     7201 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     7261 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     7321 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     7381 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     7441 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     7501 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     7561 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     7621 gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg
     7681 gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca
     7741 caggtattta ttcgcgataa gctcatggag cggcgtaacc gtcgcacagg aaggacagag
     7801 aaagcgcgga tctgggaagt gacggacaga acggtcagga cctggattgg ggaggcggtt
     7861 gccgccgctg ctgctgacgg tgtgacgttc tctgttccgg tcacaccaca tacgttccgc
     7921 cattcctatg cgatgcacat gctgtatgcc ggtataccgc tgaaagttct gcaaagcctg
     7981 atgggacata agtccatcag ttcaacggaa gtctacacga aggtttttgc gctggatgtg
     8041 gctgcccggc accgggtgca gtttgtgatg ccggagtctg atgcggttgc gatgctgaaa
     8101 caattatcct gagaataaat gccttggcct ttatatggaa atgtggaact gagtggatat
     8161 gctgtttttg tctgttaaac agagaagctg gctgttatcc actgagaagc gaacgaaaca
     8221 gtcgggaaaa tctcccatta tcgtagagat ccgcattatt aatctcagga gcctgtgtag
     8281 cgtttatagg aagtagtgtt ctgtcatgat gcctgcaagc ggtaacgaaa acgatttgaa
     8341 tatgccttca ggaacaatag aaatcttcgt gcggtgttac gttgaagtgg agcggattat
     8401 gtcagcaatg gacagaacaa cctaatgaac acagaaccat gatgtggtct gtccttttac
     8461 agccagtagt gctcgccgca gtcgagcgac agggcgaagc ccatcgatac tagcttgatt
     8521 gggatatctc gctcatgttt gtcgcgtgct atgtcttttt aggtactttg aacctacgtt
     8581 cgtacttgta taatatgatc atcgtattat cgtttttcat ccgtccagcg caaaatgcat
     8641 tagcagctag tcctagcgtg cggagctacc tggacaggtg catgacggat gcgtgtcctt
     8701 cagtgacttt ctaattaaca gtaacttctt tacttatgtt tcagtttgta agaagcggga
     8761 ttcgctcgtc gcttgacatc tgattggact gcgtcggcac gtgaaaacta cattgtgaaa
     8821 tctgctaaaa ctccgggtat ctctgacaca aaacgattcg gcttcgcaat ttcaacatta
     8881 cggtcaaggc taacgtatct ttctcggtca acttcagatt atgccgatta aattgtcgta
     8941 gctttcaagg cgttttgagt actgcggcag ttgttgaacc tgcaaggaga agatctcgac
     9001 aacagaataa agcgaaaaat gggtctcatg cactaacact caggcctccc tcataatctc
     9061 tgtttgagtt taccaacaac acatatatac atttcgacaa aatgaccact cttgacgaca
     9121 cggcttaccg gtaccgcacc agtgtcccgg gggacgccga ggccatcgag gcactggatg
     9181 ggtccttcac caccgacacc gtcttccgcg tcaccgccac cggggacggc ttcaccctgc
     9241 gggaggtgcc ggtggacccg cccctgacca aggtgttccc cgacgacgaa tcggacgacg
     9301 aatcggacga cggggaggac ggcgacccgg actcccggac gttcgtcgcg tacggggacg
     9361 acggcgacct ggcgggcttc gtggtcgtct cgtactccgg ctggaaccgc cggctgaccg
     9421 tcgaggacat cgaggtcgcc ccggagcacc gggggcacgg ggtcgggcgc gcgttgatgg
     9481 ggctcgcgac ggagttcgcc cgcgagcggg gcgccgggca cctctggctg gaggtcacca
     9541 acgtcaacgc accggcgatc cacgcgtacc ggcggatggg gttcaccctc tgcggcctgg
     9601 acaccgccct gtacgacggc accgcctcgg acggcgagca ggcgctctac atgagcatgc
     9661 cctgcccctg accgacgccg accaacaccg ccggtccgac gcggcccgac gggtccgagg
     9721 cctcggagat ctgggcccat gcggccgcaa caactacctc gactttggct gggacacttt
     9781 cagtgaggac aagaagcttc agaagcgtgc tatcgaactc aaccagggac gtgcggcaca
     9841 aatgggcatc cttgctctca tggtgcacga acagttggga gtctctatcc ttccttaaaa
     9901 atttaatttt cattagttgc agtcactccg ctttggttta agaattactc tttctctaag
     9961 tttattttga agagcgctct tctgtattaa gtaaataatt aacgaatttt tatcttccgc
    10021 tgcataaccc tgcttcgggg tcattatagc gattttttcg gtatatccat cctttttcgc
    10081 acgatataca ggattttgcc aaagggttcg tgtagacttt ccttggtgta tccaacggcg
    10141 tcagccgggc aggataggtg aagtaggccc acccgcgagc gggtgttcct tcttcactgt
    10201 cccttattcg cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct ggccggctac
    10261 cgccggcgta acagatgagg gcaagcggat ggctgatgaa accaagccaa ccaggaaggg
    10321 cagcccacct atcaaggtgt actgccttcc agacgaacga aaagcgattg aggaaaaggc
    10381 ggcggcggcc ggcatgagcc tgtcggccta cctgctggcc gtcggccagg gctacaaaat
    10441 cacgggcgtc gtggactatg agcacgtccg cgagctggcc cgcatcaatg gcgacctggg
    10501 ccgcctgggc ggcctgctga aactctggct caccgacgac ccgcgcacgg cgcggttcgg
    10561 tgatgccacg atcctcgccc tgctggcgaa gatcgaagag aagcaggacg agcttggcaa
    10621 ggtcatgatg ggcgtggtcc gcccgagggc agagccatga cttttttagc cgctaaaacg
    10681 gccggggggt gcgcgtgatt gccaagcacg tccccatgcg ctccatcaag aagaggcact
    10741 tcgagctgta agtacatcac cgacgagcaa ggcaagacga tccgcgcctg ttttattgag
    10801 aacgttgttc gtgttggcct caatggtagc gatgcgtcat tcagcgaagt tctggtgctg
    10861 atgatgtggt tcgctttgcc actggtcaat gtggtaagcc cgtgtaatgt cagtaacctt
    10921 tttactgatc tcagcttgag cacggtcgct gatgagctta tccatggccc cacggtaacg
    10981 gatatgatcc tctagggcgt tgacaaattc tttgtcggtt ttcatggggt agacgtcagt
    11041 gaccagggaa cgtctcccta caaaaagttg cgcatacttt gctccgttgt cgacggcagg
    11101 ggtgtcggac cagattgtat ctgtggcaac ggcctcattg cgtcaatgga cctttaaagc
    11161 cgggaaacga gacttgaaat gtttacgtag aggtgcatta tagacctcac gcgcatattg
    11221 agtggttgca aaaatggttt tgcgaacggt atcactggag acccatgcca ggcaaggacg
    11281 gagggcatca taatcatgtt cattgcgttg gacagcatgc ttgttacacg taagtatacg
    11341 gtcgagagca tcgtgacgtt gagaattaca gatggcaacg tgtcggtgca tgtattggcc
    11401 gagattgtca aatcgtggct caatatatgg catgccaggt aagtcatgga tgtcggtatg
    11461 ccattcagcc ttcatgtcga tttcgttgtc gagaactgat gggtcccagt caacgtcaga
    11521 agtaagaaca acatggggaa gggaatgaag ttcatgactg gtgggtgcgc gcatatccat
    11581 gtacggaaga ccctgtcgta tgtttagtgg gatgatgtaa tcatccaggg taacaatacg
    11641 ttggttgccg cccacggtac gagaccgatc ttgaacacaa ttgtggcaat gttcaagttg
    11701 ggcgctggaa tggatggttt tacctttacc aagatgcgcg tattgatgca tgataacaac
    11761 aatgggccct cgttgcgatt cgacaagacc ggcagccgtg acaatgtcta gattggacaa
    11821 ggtatgttca tttataccgg ttatgttggc tgaacgtcca gttttgtgca gtacggtaac
    11881 atcactcccc gcaagtccac cgttggcacc gcgatcgaca agtgcagatg tggtattttg
    11941 tactgtgtgt cgggagactt ggtactgtaa cacatttgac tgaagtgact tggtggtacc
    12001 atgaggacgg agggaacccg ttgatacggt gttgcgtgac gctgcaagta ccttacgtat
    12061 gtcgcctgga tccatacgtc caacccgatc agaaagatgt gctaggagtt ccgtttctgg
    12121 tgcacaatca tgaaaggtat ccacgggagt tccattgttg tcgtccgggg ctgcgtcgcg
    12181 actgccagtt gcatgtacat gcgccatcaa gggacgtgcc aatccattac taaccgggag
    12241 aaccttagct ggggcagcca atccctgaag tatagcctta gcatcatctg agagctggtt
    12301 ccacatatct ctagggatat aaggtcgttc acggttagtg ggcgtactat gatgtcggtt
    12361 attagtacaa ttctccctgc gggcatgcgc attggcttca tagagtacgg aaggtgacaa
    12421 gtcaatattg tagttaacat ccggatcagt gtcaaagtct gtaggatggt aagaaagatc
    12481 agtagaatga atactacgct tttccttggg actacgggaa ttagagaagt tgtttccttt
    12541 attgtagagt gacgctgaag caagtagaag actaaggtaa ctctcgtagc taataggatt
    12601 acctcctttg gctaggtcaa gagtggctgt gatcttcact tgacaaagtt ccggtacatt
    12661 gtggacagca ttctccaaaa gactaagaca cagttgcttt gggagttgct cagccattgg
    12721 tacagtattg tggtagatac aaaggtggtt cttccaatga aggataaatc cttctgctgt
    12781 gcctgtccat gaggatccat atttcgccgt agttaggtaa ccaagcgtgg tggctgaact
    12841 gatcttcgca cttgctgatt ccgtatagtg tttgacaact ttacaaaaca cttcttgcgc
    12901 agttcgctct agcctggaca gagaagggac aatcttcgtc gttaagactc gtgcgaatag
    12961 caaaagatca caaaatagca catcggcacc gaccaacgat tatttccaag gaaaaaaaga
    13021 atgcttcact acaagaaatt gtgtcatccc tatacagagt cttgttactg tgacagaaaa
    13081 ttgatggaag atgtggcgga ttgcctttac actagccaac ttgttcgact aattgcagct
    13141 tcttctgaga ggcttcaccg agtaacgcga agaacaccgg tgtctcgtac atgctcgtcg
    13201 gtgaacgctc gtccaatgac accccccact ttgtatcaat atcccaactt ggtagtgaac
    13261 tggaatgata catgcaattt cgcgccgcat caacagccac gggccaccat cgacgaatag
    13321 actcggtcga gctggttgcc ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg
    13381 cgccagaaac gccgtcgaag ccgtgtgcga gacaccgcgg ccggccgccg gcgttgtgga
    13441 tacctcgcgg aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg
    13501 gccgactcac ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg
    13561 tggagctggc cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag
    13621 atgatgtgga caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact
    13681 actgacagat gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg
    13741 gcgcacctat tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt
    13801 ttccgcccgt ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt
    13861 ataaaccttg tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg
    13921 ggtgcccccc cttctcgaac cctcccggtc gagtgagcga ggaagcacca gggaacagca
    13981 cttatatatt ctgcttacac acgatgcctg aaaaaacttc ccttggggtt atccacttat
    14041 ccacggggat atttttataa ttattttttt tatagttttt agatcttctt ttttagagcg
    14101 ccttgtaggc ctttatccat gctggttcta gagaaggtgt tgtgacaaat tgccctttca
    14161 gtgtgacaaa ttaccctcaa atgacagtcc tgtctgtgac aaattgccct taaccctgtg
    14221 acaaattgcc ctcagaagaa gctgtttttt cacaaagtta tccctgctta ttgactcttt
    14281 tttatttagt gtgacaatct aaaaacttgt cacacttcac atggatctgt c
//
