LOCUS       sequence-419870-        9333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses Wild type BRD4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Shu S, Lin CY, He HH, Witwicki RM, Tabassum DP, Roberts JM, 
            Janiszewska M, Huh SJ, Liang Y, Ryan J, Doherty E, Mohammed H, Guo 
            H, Stover DG, Ekram MB, Peluffo G, Brown J, D'Santos C, Krop IE, 
            Dillon D, McKeown M, Ott C, Qi J, Ni M, Rao PK, Duarte M, Wu SY, 
            Chiang CM, Anders L, Young RA, Winer EP, Letai A, Barry WT, Carroll 
            JS, Long HW, Brown M, Liu XS, Meyer CA, Bradner JE, Polyak K
  TITLE     Response and resistance to BET bromodomain inhibitors in 
            triple-negative breast cancer.
  JOURNAL   Nature. 2016 Jan 21;529(7586):413-7. doi: 10.1038/nature16508. Epub 
            2016 Jan 6.
  PUBMED    26735014
REFERENCE   2  (bases 1 to 9333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature16508"; journalName: "Nature"; date: "2016-01-21- 
            21"; volume: "529"; issue: "7586"; pages: "413-7"
COMMENT     Sequence Label: pcDNA5-Flag-BRD4-WT
FEATURES             Location/Qualifiers
     source          1..9333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1211..1230)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1402..1781
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1782..1985
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1936..1956
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1987..2005
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2008..2026
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2036..2060
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2159..2182
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4400..4417
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6452..6469)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6458..6682
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6815..6834)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6966..7013
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7021..8043
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7021..8043
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8173..8294
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8210..8229)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8264..8283
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8343..8359)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8343..8359)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8356..8378)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8367..8383
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8391..8397,8398..8415,8416..8421))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8436..8457
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8574..8591)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8745..9333)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8825..8844)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     1201 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     1261 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     1321 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     1381 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     1441 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     1501 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     1561 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     1621 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     1681 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     1741 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     1801 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     1861 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     1921 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     1981 gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc
     2041 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
     2101 gacaccggga ccgatccagc ctccggactc tagcgtttaa acttaagctt ccaccatgga
     2161 ttacaaggat gacgacgata agggatctat gtctgcggag agcggccctg ggacgagatt
     2221 gagaaatctg ccagtaatgg gggatggact agaaacttcc caaatgtcta caacacaggc
     2281 ccaggcccaa ccccagccag ccaacgcagc cagcaccaac cccccgcccc cagagacctc
     2341 caaccctaac aagcccaaga ggcagaccaa ccaactgcaa tacctgctca gagtggtgct
     2401 caagacacta tggaaacacc agtttgcatg gcctttccag cagcctgtgg atgccgtcaa
     2461 gctgaacctc cctgattact ataagatcat taaaacgcct atggatatgg gaacaataaa
     2521 gaagcgcttg gaaaacaact attactggaa tgctcaggaa tgtatccagg acttcaacac
     2581 tatgtttaca aattgttaca tctacaacaa gcctggagat gacatagtct taatggcaga
     2641 agctctggaa aagctcttct tgcaaaaaat aaatgagcta cccacagaag aaaccgagat
     2701 catgatagtc caggcaaaag gaagaggacg tgggaggaaa gaaacaggga cagcaaaacc
     2761 tggcgtttcc acggtaccaa acacaactca agcatcgact cctccgcaga cccagacccc
     2821 tcagccgaat cctcctcctg tgcaggccac gcctcacccc ttccctgccg tcaccccgga
     2881 cctcatcgtc cagacccctg tcatgacagt ggtgcctccc cagccactgc agacgccccc
     2941 gccagtgccc ccccagccac aacccccacc cgctccagct ccccagcccg tacagagcca
     3001 cccacccatc atcgcggcca ccccacagcc tgtgaagaca aagaagggag tgaagaggaa
     3061 agcagacacc accaccccca ccaccattga ccccattcac gagccaccct cgctgccccc
     3121 ggagcccaag accaccaagc tgggccagcg gcgggagagc agccggcctg tgaaacctcc
     3181 aaagaaggac gtgcccgact ctcagcagca cccagcacca gagaagagca gcaaggtctc
     3241 ggagcagctc aagtgctgca gcggcatcct caaggagatg tttgccaaga agcacgccgc
     3301 ctacgcctgg cccttctaca agcctgtgga cgtggaggca ctgggcctac acgactactg
     3361 tgacatcatc aagcacccca tggacatgag cacaatcaag tctaaactgg aggcccgtga
     3421 gtaccgtgat gctcaggagt ttggtgctga cgtccgattg atgttctcca actgctataa
     3481 gtacaaccct cctgaccatg aggtggtggc catggcccgc aagctccagg atgtgttcga
     3541 aatgcgcttt gccaagatgc cggacgagcc tgaggagcca gtggtggccg tgtcctcccc
     3601 ggcagtgccc cctcccacca aggttgtggc cccgccctca tccagcgaca gcagcagcga
     3661 tagctcctcg gacagtgaca gttcgactga tgactctgag gaggagcgag cccagcggct
     3721 ggctgagctc caggagcagc tcaaagccgt gcacgagcag cttgcagccc tctctcagcc
     3781 ccagcagaac aaaccaaaga aaaaggagaa agacaagaag gaaaagaaaa aagaaaagca
     3841 caaaaggaaa gaggaagtgg aagagaataa aaaaagcaaa gccaaggaac ctcctcctaa
     3901 aaagacgaag aaaaataata gcagcaacag caatgtgagc aagaaggagc cagcgcccat
     3961 gaagagcaag ccccctccca cgtatgagtc ggaggaagag gacaagtgca agcctatgtc
     4021 ctatgaggag aagcggcagc tcagcttgga catcaacaag ctccccggcg agaagctggg
     4081 ccgcgtggtg cacatcatcc agtcacggga gccctccctg aagaattcca accccgacga
     4141 gattgaaatc gactttgaga ccctgaagcc gtccacactg cgtgagctgg agcgctatgt
     4201 cacctcctgt ttgcggaaga aaaggaaacc tcaagctgag aaagttgatg tgattgccgg
     4261 ctcctccaag atgaagggct tctcgtcctc agagtcggag agctccagtg agtccagctc
     4321 ctctgacagc gaagactccg aaacagagat ggctccgaag tcaaaaaaga aggggcaccc
     4381 cgggagggag cagaagaagc accatcatca ccaccatcag cagatgcagc aggccccggc
     4441 tcctgtgccc cagcagccgc ccccgcctcc ccagcagccc ccaccgcctc cacctccgca
     4501 gcagcaacag cagccgccac ccccgcctcc cccaccctcc atgccgcagc aggcagcccc
     4561 ggcgatgaag tcctcgcccc cacccttcat tgccacccag gtgcccgtcc tggagcccca
     4621 gctcccaggc agcgtctttg accccatcgg ccacttcacc cagcccatcc tgcacctgcc
     4681 gcagcctgag ctgccccctc acctgcccca gccgcctgag cacagcactc caccccatct
     4741 caaccagcac gcagtggtct ctcctccagc tttgcacaac gcactacccc agcagccatc
     4801 acggcccagc aaccgagccg ctgccctgcc tcccaagccc gcccggcccc cagccgtgtc
     4861 accagccttg acccaaacac ccctgctccc acagcccccc atggcccaac ccccccaagt
     4921 gctgctggag gatgaagagc cacctgcccc acccctcacc tccatgcaga tgcagctgta
     4981 cctgcagcag ctgcagaagg tgcagccccc tacgccgcta ctcccttccg tgaaggtgca
     5041 gtcccagccc ccaccccccc tgccgccccc accccacccc tctgtgcagc agcagctgca
     5101 gcagcagccg ccaccacccc caccacccca gccccagcct ccaccccagc agcagcatca
     5161 gccccctcca cggcccgtgc acttgcagcc catgcagttt tccacccaca tccaacagcc
     5221 cccgccaccc cagggccagc agccccccca tccgccccca ggccagcagc cacccccgcc
     5281 gcagcctgcc aagcctcagc aagtcatcca gcaccaccat tcaccccggc accacaagtc
     5341 ggacccctac tcaaccggtc acctccgcga agccccctcc ccgcttatga tacattcccc
     5401 ccagatgtca cagttccaga gcctgaccca ccagtctcca ccccagcaaa acgtccagcc
     5461 taagaaacag gagctgcgtg ctgcctccgt ggtccagccc cagcccctcg tggtggtgaa
     5521 ggaggagaag atccactcac ccatcatccg cagcgagccc ttcagcccct cgctgcggcc
     5581 ggagcccccc aagcacccgg agagcatcaa ggcccccgtc cacctgcccc agcggccgga
     5641 aatgaagcct gtggatgtcg ggaggcctgt gatccggccc ccagagcaga acgcaccgcc
     5701 accaggggcc cctgacaagg acaaacagaa acaggagccg aagactccag ttgcgcccaa
     5761 aaaggacctg aaaatcaaga acatgggctc ctgggccagc ctagtgcaga agcatccgac
     5821 caccccctcc tccacagcca agtcatccag cgacagcttc gagcagttcc gccgcgccgc
     5881 tcgggagaaa gaggagcgtg agaaggccct gaaggctcag gccgagcacg ctgagaagga
     5941 gaaggagcgg ctgcggcagg agcgcatgag gagccgagag gacgaggatg cgctggagca
     6001 ggcccggcgg gcccatgagg aggcacgtcg gcgccaggag cagcagcagc agcagcgcca
     6061 ggagcaacag cagcagcagc aacagcaagc agctgcggtg gctgccgccg ccaccccaca
     6121 ggcccagagc tcccagcccc agtccatgct ggaccagcag agggagttgg cccggaagcg
     6181 ggagcaggag cgaagacgcc gggaagccat ggcagctacc attgacatga atttccagag
     6241 tgatctattg tcaatatttg aagaaaatct tttctgagcg cacctaggtg gcttctgact
     6301 ttgattttct ggcaaaacat tgactttcca tagtgttagg ggcggtggtg gaggtgggat
     6361 cagcggccag gggatgcctc agggcctggc cctcctgcat gcaagcttca gctgctcgag
     6421 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     6481 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     6541 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     6601 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     6661 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     6721 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     6781 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     6841 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     6901 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     6961 acctagaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttccttggcc
     7021 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     7081 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     7141 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     7201 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     7261 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     7321 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
     7381 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     7441 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     7501 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     7561 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     7621 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     7681 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     7741 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     7801 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     7861 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     7921 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     7981 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     8041 tagcacgtac tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     8101 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     8161 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8221 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8281 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     8341 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8401 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     8461 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     8521 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     8581 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     8641 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8701 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8761 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8821 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8881 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8941 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9001 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9061 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9121 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9181 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9241 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9301 cagcagatta cgcgcagaaa aaaaggatct caa
//
