LOCUS       sequence-419887-       16913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene targeting vector for the mouse Rosa26 locus, including a CAG 
            promoter, loxP flanked stop cassette, GFP, for gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16913)
  AUTHORS   Chu VT, Weber T, Graf R, Sommermann T, Petsch K, Sack U, Volchkov P,
            Rajewsky K, Kuhn R
  TITLE     Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in 
            C57BL/6 zygotes.
  JOURNAL   BMC Biotechnol. 2016 Jan 16;16(1):4. doi: 10.1186/s12896-016-0234-4.
  PUBMED    26772810
REFERENCE   2  (bases 1 to 16913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-01-16"; volume: "16(1):4. doi"; pages: " 
            10.1186/s12896-016-0234-4"
COMMENT     Sequence Label: pR26 CAG/GFP Dest
FEATURES             Location/Qualifiers
     source          1..16913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1140..1163
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    1248..5589
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     polyA_signal    5679..5903
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5922..5938
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5963..6010)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(join(6035..6748,6749..6751,6752..6754))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(6038..6748,6749..6751,6752..6754))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6080..6101)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6429..6448
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6688..6709
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    6766..7318
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6928..6947)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     7157..7174
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    complement(7363..7410)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    7445..7569
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(7610..7915)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(7712..7731)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(8257..8916)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     8831..8850
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(8970..8976,8977..8994,8995..9000))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(9025..9149)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    9162..9195
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    9215..9349
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9239..9258)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9293..9312
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     intron          9924..9989
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10802..11605
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10865..10884)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11475..11494
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11612..11660
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    11667..11700
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(11736..11755)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          11763..12779
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(11817..11839)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(12780..13061)
                     /label=chicken beta-actin promoter
     enhancer        13063..13442
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    13451..13658
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    13672..13879
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    join(13898..13935,13936..14001)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     misc_feature    14008..15092
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     primer_bind     complement(15110..15129)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(15111..15129)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(15139..15156)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(15139..15155)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15148..15170)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(15296..15751)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15433..15454)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15645..15664
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        15778..15882
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(15883..15951,15952..16743)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(16101..16120)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tgggtaccgc ggccgcgatc gccctgcagg gcggcggcgc
     1081 ctaccggtgg atgtggaatg tgtgcgaggc cagaggccac ttgtgtagcg ccaagtgccc
     1141 agcggggctg ctaaagcgca tgctccagac tgccttggga aaagcgcctc ccctacccgg
     1201 tagaattcga tatcaagctt atcgataccg tcgacggtat cgataagcta gatgtcctga
     1261 aatatttcta tcagaacaag gtagtataaa gctggtaggt atacaaaacg ctagactagt
     1321 ttctatccct gacccttaat ctgctagtat atccgtagga agttgcttaa gtgccactag
     1381 taccaacagc ctctgttaga gtgaacagta gattttaatg aagggccaat aaagaccact
     1441 taagactact gactgaatta aaaatccacc cagtggagtt agtattggtt atagttatat
     1501 aacaaaatat ccaacttagc caggcgtggt ggtgcacgcc tttaatcccg gcactcagga
     1561 ggcagaggca ggcggatttc tgagttcgag gccagcctgg tctacaaagt gagttccagg
     1621 acagccaggg ctatacagag aaaccctgtc tcgaaaaacc aaaaaaaaaa aaaaaaaagg
     1681 aaaaaaaata tggcttgaaa acatgctatt gttgtcaaaa ggagatccaa acaagtattt
     1741 ctttgaacaa aaattatgta gaaaacaggg tgataaagaa caagggcttt ttaaaaaagg
     1801 ccagcattat ttgagatata caagtgactc caattcaagc ctaaaggcca ctcaatgctc
     1861 actaacagtg ctaatcagaa tcaattcctt agcccagtca aaaatagtcc tgaatatctt
     1921 ctggataaac atgtcctcca acatataaac tagtatacac taacaaaacg tctcaacttc
     1981 aaggtgaaat gcttgactcc tagacttgtg acccagcaaa gtgctctata ggtagggtta
     2041 ctaggtcaga gagtcttgcc tgcaaaccac aattatacaa ttccaccaaa tgtaagacat
     2101 gtaaaaatga cttatttagc tcaggttttg aaaattaaaa atcaaaccca caaagaccta
     2161 actttcacat taagtacaaa tgtttaacat atataacatg ttttgccttg atatatgaaa
     2221 tcatatgaca tcacctgaca ggaaacttaa gtttatttga acaacaatca gcctaaggta
     2281 ggcctagcac atgatcagga tagtgcaggg aaacccaaag aagtgcttct gagtataaac
     2341 caagagacct cgaaatagca gctttgttct gtatctcatg ggctttaact atcacttgaa
     2401 aacaatttca caaatcacaa aaccaacatt tgtttagttt ccaactctgc ctattcaccg
     2461 gagaaatcca tgtcccaatt tgatggggga aaacttgaat gaaatattat ttggaatatt
     2521 ttaatatccc tgtgtatatg cagatggttt aaagacaggt aaccttaact gtagtttctt
     2581 ggttgtctgc cttgtttttt gaaatatgct ctcaccagga gcctgccaag taactactct
     2641 tgtgtgctca gcaagtccta gggatccctt aagcatgctc taacaggcct ggcttttttt
     2701 tttaaaaagt aggttgcggt gataataatt caagttttca tcacttgaaa cacattttac
     2761 caactatcac ccaagctccc ttagtgtgtc ccctataaaa gaatctgacc tgcaagttcc
     2821 aaaagtatta ctacatttta accttagtat tcaggagaca aaagcaggca gaactttagt
     2881 ctacatatct tgttgcagat caggcagaga tacatagtga gaccctgcct caggaaaagc
     2941 tatcattttt atattcacag taagcaaatt aacattaaaa gtcagaaatt cttaatttga
     3001 ctttggctgt gaagaatttg gattctctgg atttgaaact agaaaatctg attaagcgat
     3061 gcaacagttt ctgtatacta aaactctaag acccttggtt ctaaagatac cacatttaaa
     3121 aaaaaagtta agtataagac aaaaagcctt aatagctctt ttatagggga gggactcatt
     3181 taatattagt ccacctcact cctcataact attttatgag gtttattata taacccattt
     3241 tttaaaaaaa aaattaaagg acaattttag tgtttgaaag atttcccaac cccaccctgg
     3301 aaatcaggct gcaaatctca gcagctgccc ttagaaggca aaaagccatg ccatttaagc
     3361 catgggaagt tagtagcaaa caagagacca catggcagga aggaggcttt aaagaaagcc
     3421 cacagtgtca aaagacccaa ccaacagcag agacagaaaa aataataccc aaatctcatc
     3481 agaaaggtag acggatttag ccacatccat agtggctcat tagggaatgc ttcaattata
     3541 attttatgaa atctcagagc ccccctcccc aacagggttt ctgtgtgtat tcctggctat
     3601 cctagataga acttgatgtg tagaccaggc tgggctcaaa ctcagagatc tgcctgtctc
     3661 tgcctccaga gtgctgggat taaaggcata aaggcaagca ccaccactgg ctggctaaac
     3721 tctggcccta catttttgaa atccaaaatt atcattactt gctttcatgt ttagtaatgg
     3781 ctgcagactt agctttcagc tttgtataga tgaagcacaa aagttaagta aagctagcag
     3841 tttaaacaaa taggatacta gaagttcaat ttttatttct ttattgctta gtgtaagaga
     3901 aaagacatta aagtctgcat tggtcttctg tatcccacaa gtctgcagtt atggctcctc
     3961 tgtccacagt tacacttcaa ataaaaatta gttccttggt aacatttcca gtgcttaaag
     4021 agtaatttga ttattggcta ccatattgga acaaacacaa agtatttcat tacttcagta
     4081 aattctactg gagacagtat tctctttaaa aaaaaaaaaa agaagaagaa ggaggattta
     4141 tttatttaat gtgaatacac ttgtggtctt cagacacacc agaagagggc atcagatccc
     4201 attacagatg gttgtgagcc accatgtggt tgctgggaat tgaactcagg acttctggaa
     4261 gagcagtctg tgctcttgaa ctccgagcca cctctccagc ccctggatag tattattata
     4321 gagcacaagc acacacaaca caacaaccta aaaaccaagc ttcacaaata catttgttgc
     4381 ttaatcattt tacttgtgtt tggctacaag tcaagcaaaa ttataggtcc tgaagaagct
     4441 tggcaaaatc acatttagac cagcaataac gtgtagaatg ccatgagtca agccagtcca
     4501 agagaaagca ctgcttaaat agaaaaaata caagctcaca agaccttagg tcaggaaaga
     4561 caacaaacac ctgaactttg cattccaaaa ggaaccacct tttacagatg tgtacaatga
     4621 caaaatttta gtaagcagta atcaatacca tgtggctcaa taatgaaata taccttttaa
     4681 tgtcttaaaa tatcagcctg gcaatatgta agatacatca ggtaatattg ggggaggaga
     4741 catccacctg gaaaccatta atggttaata gctcagttta taaatggaga aaaaggagag
     4801 aggcattcat gggagtggaa agttaagctt taggttggat tctcaataca tctattgttg
     4861 taaaactccc tggactgaga ataggcccaa atgtggaaca ccacctgacg ggagaggtga
     4921 tagacactga aaattaagga tcaaggcaaa ggatcaacaa aaagtgtact aaggagttat
     4981 aaaagaactg cagtgttgag gccccagcta cagcctcgat ttgtggtgta tgtaactaat
     5041 ctgtctggtt tcatgagtca tcagacttct aagatcagga aagggaaaat gccaatgctc
     5101 tgtctagggg ttggataagc cagtataata aatgaaaatg gggctaaaat gagtgttcta
     5161 aaataccttt tgataaggct gcagaaggag cgggagaaat ggatatgaag tactgggctc
     5221 tttaaaaatg attaaaattc tgcttacata gtctaactcg cgacactgta atttcatact
     5281 gtagtaagga tctcaagcag gagagtataa aactcgggtg agcatgtctt taatctacct
     5341 cgatggaaaa tactccgagg cggatcacaa gcaataataa cctgtagttt tgctgcataa
     5401 aaccccagat gactacctat cctcccattt tccttatttg cccctattaa aaaacttccc
     5461 gacaaaaccg aaaatctgtg ggaagtcttg tccctccaat tttacacctg ttcaattccc
     5521 ctgcaggaca acgcccacac accaggttag cctttaagcc tgcccagaag actcccgccc
     5581 atcttctagc gaattcgata tcggcgcggc ggccgctcta gcctcgaggc tagaactagt
     5641 ggatctcgag ccccagctgg ttctttccgc ctcagaagcc atagagccca ccgcatcccc
     5701 agcatgcctg ctattgtctt cccaatcctc ccccttgctg tcctgcccca ccccaccccc
     5761 cagaatagaa tgacacctac tcagacaatg cgatgcaatt tcctcatttt attaggaaag
     5821 gacagtggga gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga
     5881 tggctggcaa ctagaaggca cagtcgaggc tgatcggccg ctctagaact agtggatccc
     5941 ccgggctgca tccaacctcg aggaagttcc tatactttct agagaatagg aacttcggaa
     6001 taggaacttc gttgggagct ctccggatcc cggattactt gtacagctcg tccatgccga
     6061 gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg
     6121 ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc
     6181 cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga
     6241 tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg gccatgatat
     6301 agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct
     6361 tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct
     6421 cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc
     6481 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga
     6541 agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc
     6601 cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
     6661 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
     6721 ccccggtgaa cagctcctcg cccttgctca ccatggttgt ggccatatta tcatcgtgtt
     6781 tttcaaagga aaaccacgtc cccgtggttc ggggggccta gacgtttttt taacctcgac
     6841 taaacacatg taaagcatgt gcaccgaggc cccagatcag atcccataca atggggtacc
     6901 ttctgggcat ccttcagccc cttgttgaat acgcttgagg agagccattt gactctttcc
     6961 acaactatcc aactcacaac gtggcactgg ggttgtgccg cctttgcagg tgtatcttat
     7021 acacgtggct tttggccgca gaggcacctg tcgccaggtg gggggttccg ctgcctgcaa
     7081 agggtcgcta cagacgttgt ttgtcttcaa gaagcttcca gaggaactgc ttccttcacg
     7141 acattcaaca gaccttgcat tcctttggcg agaggggaaa gacccctagg aatgctcgtc
     7201 aagaagacag ggccaggttt ccgggccctc acattgccaa aagacggcaa tatggtggaa
     7261 aataacatat agacaaacgc acaccggcct tattccaagc ggcttcggcc agtaacgtta
     7321 gggggggggg gggggggggg gcggaattgg ggtaccctcg aggaagttcc tatactttct
     7381 agagaatagg aacttcggaa taggaacttc ggtaccgaat tgatcgcggc cgcggcgcgc
     7441 catcaccact ttgtacaaga aagctgaacg agaaacgtaa aatgatataa atatcaatat
     7501 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
     7561 gtcactatgg tcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc
     7621 agaacatcag gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt
     7681 cttccccgat aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt
     7741 catccccgat atgcaccacc gggtaaagtt cacgggagac tttatctgac agcagacgtg
     7801 cactggccag ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat
     7861 ccacaaacag acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac
     7921 cagcccctgt tctcgtcagc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc
     7981 atcccttcct gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc
     8041 atggttgtgc ttaccagacc ggagatattg acatcatata tgccttgagc aactgatagc
     8101 tgtcgctgtc aactgtcact gtaatacgct gcttcatagc atacctcttt ttgacatact
     8161 tcgggtatac atatcagtat atattcttat accgcaaaaa tcagcgcgca aatacgcata
     8221 ctgttatctg gcttttagta agccggatcc acgcgtttac gccccgccct gccactcatc
     8281 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     8341 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     8401 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     8461 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata
     8521 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     8581 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     8641 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa
     8701 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     8761 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     8821 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     8881 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     8941 tctcgacgga tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa
     9001 gcctggggtg cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat
     9061 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
     9121 tctcgttcag cttttttgta caaacttgtg atggcgcgcc gataacttcg tataatgtat
     9181 gctatacgaa gttataagct tacttaccat gtcagatcca gacatgataa gatacattga
     9241 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     9301 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     9361 ttgcattcat tctatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     9421 aaacctctac aaatgtggta tggctgatta tgatctctag tcaaggcact atacatcaaa
     9481 tattccttat taaccccttt acaaattaaa aagctaaagg tacacaattt ttgagcatag
     9541 ttattaatag cagacactct atgcctgtgt ggagtaagaa aaaacagtat gttatgatta
     9601 taactgttat gcctacttat aaaggttaca gaatattttt ccataatttt cttgtatagc
     9661 agtgcagctt tttcctttgt ggtgtaaata gcaaagcaag caagagttct attactaaac
     9721 acagcatgac tcaaaaaact tagcaattct gaaggaaagt ccttggggtc ttctaccctt
     9781 ctcttctttt ttggaggagt agaatgttga gagtcagcag tagcctcatc atcactagat
     9841 ggcatttctt ctgagcaaaa caggttttcc tcattaaagg cattccacca ctgctcccat
     9901 tcatcagttc cataggttgg aatctaaaat acacaaacaa ttagaatcag tagtttaaca
     9961 cattatacac ttaaaaattt tatatttacc ttagagcttt aaatctctgt aggtagtttg
    10021 tccaattatg tcacaccaca gaagtaaggt tccttcacaa agatccctcg agaaaaaaaa
    10081 tataaaagag atggaggaac gggaaaaagt tagttgtggt gataggtggc aagtggtatt
    10141 ccgtaagaac aacaagaaaa gcatttcata ttatggctga actgagcgaa caagtgcaaa
    10201 atttaagcat caacgacaac aacgagaatg gttatgttcc tcctcactta agaggaaaac
    10261 caagaagtgc cagaaataac agtagcaact acaataacaa caacggcggc tacaacggtg
    10321 gccgtggcgg tggcagcttc tttagcaaca accgtcgtgg tggttacggc aacggtggtt
    10381 tcttcggtgg aaacaacggt ggcagcagat ctaacggccg ttctggtggt agatggatcg
    10441 atggcaaaca tgtcccagct ccaagaaacg aaaaggccga gatcgccata tttggtgtcc
    10501 ccgagtcgac tttaatcact agtcgagcag tgtggttttc aagaggaagc aaaaagcctc
    10561 tccacccagg cctggaatgt ttccacccaa tgtcgagcag tgtggttttg caagaggaag
    10621 caaaaagcct ctccacccag gcctggaatg tttccaccca atgtcgagca aaccccgccc
    10681 agcgtcttgt cattggcgaa ttcgaacacg cagatgcagt cggggcggcg cggtcccagg
    10741 tccacttcgc atattaaggt gacgcgtgtg gcctcgaaca ccgagcgacc ctgcagccaa
    10801 tatgggatcg gccattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
    10861 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
    10921 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
    10981 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
    11041 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
    11101 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
    11161 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
    11221 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
    11281 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
    11341 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
    11401 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
    11461 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
    11521 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
    11581 tcgccttctt gacgagttct tctgagggga tcggcaataa aaagacagaa taaaacgcac
    11641 gggtgttggg tcgtttgttc ggatccataa cttcgtataa tgtatgctat acgaagttat
    11701 ttaattaaga attctttgcc aaaatgatga gacagcacaa taaccagcac gttgcccagg
    11761 agctgtagga aaaagaagaa ggcatgaaca tggttagcag aggctctaga gccgccggtc
    11821 acacgccaga agccgaaccc cgccctgccc cgtccccccc gaaggcagcc gtccccccgc
    11881 ggacagcccc gaggctggag agggagaagg ggacggcggc gcggcgacgc acgaaggccc
    11941 tccccgccca tttccttcct gccggcgccg caccgcttcg ccccgcgccc gctagagggg
    12001 gtgcggcggc gcctcccaga tttcggctcc gcacagattt gggacaaagg aagtccctgc
    12061 gccctctcgc acgattacca taaaaggcaa tggctgcggc tcgccgcgcc tcgacagccg
    12121 ccggcgctcc gggggccgcc gcgcccctcc cccgagccct ccccggcccg aggcggcccc
    12181 gccccgcccg gcacccccac ctgccgccac cccccgcccg gcacggcgag ccccgcgcca
    12241 cgccccgtac ggagccccgc acccgaagcc gggccgtgct cagcaactcg gggagggggg
    12301 tgcagggggg gttgcagccc gaccgacgcg cccacacccc ctgctcaccc ccccacgcac
    12361 acaccccgca cgcagccttt gttcccctcg cagccccccc cgcaccgcgg ggcaccgccc
    12421 ccggccgcgc tcccctcgcg cacactgcgg agcgcacaaa gccccgcgcc gcgcccgcag
    12481 cgctcacagc cgccgggcag cgcggagccg cacgcggcgc tccccacgca cacacacacg
    12541 cacgcacccc ccgagccgct ccccccgcac aaagggccct cccggagccc ctcaaggctt
    12601 tcacgcagcc acagaaaaga aacaagccgt cattaaacca agcgctaatt acagcccgga
    12661 ggagaagggc cgtcccgccc gctcacctgt gggagtaacg cggtcagtca gagccggggc
    12721 gggcggcgcg aggcggcggc ggagcggggc acggggcgaa ggcagcgcgc agcgactccc
    12781 gcccgccgcg cgcttcgctt tttatagggc cgccgccgcc gccgcctcgc cataaaagga
    12841 aactttcgga gcgcgccgct ctgattggct gccgccgcac ctctccgcct cgccccgccc
    12901 cgcccctcgc cccgccccgc cccgcctggc gcgcgccccc cccccccccc ccccgccccc
    12961 atcgctgcac aaaataatta aaaaataaat aaatacaaaa ttgggggtgg ggaggggggg
    13021 ggagatgggg agagtgaagc agaacgtggg gctcacctcg accatggtaa tagcgatgac
    13081 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
    13141 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
    13201 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
    13261 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
    13321 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
    13381 tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg
    13441 tcgacccgca tccccagcat gcctgctatt gtcttcccaa tcctccccct tgctgtcctg
    13501 ccccacccca ccccccagaa tagaatgaca cctactcaga caatgcgatg caatttcctc
    13561 attttattag gaaaggacag tgggagtggc accttccagg gtcaaggaag gcacggggga
    13621 ggggcaaaca acagatggct ggcaactaga aggcacagtc gctagaccgc atccccagca
    13681 tgcctgctat tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga
    13741 atagaatgac acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca
    13801 gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc
    13861 tggcaactag aaggcacagt cctcgagtta attaaccact ggaaagaccg cgaagagttt
    13921 gtcctcaacc gcgagctgtg gaaaaaaaag ggacaggata agtatgacat catcaaggaa
    13981 accctggact actgcgccct acagatgcta gaaagactgg agttgcagat cacgagggaa
    14041 gagggggaag ggattctccc aggcccaggg cggtcctcag aagccaggag gcagcagaga
    14101 actcccagaa aggtattgca acactcccct cccccctccg gagaagggtg cggccttctc
    14161 cccgcctact ccactgcagc tcccttactg ataacaactc agagcgactt tgggagagca
    14221 agtgcttcct gcctccaaaa cagcccaact gagccctcgc tccttccctc cactccccgg
    14281 agtgcgcgat ggaggtctgg ctcagcacgc ccctcttgag gcaactcaag tcggaaacgt
    14341 gcttgcaccc gccccgcagc cgctcagccc tactgcccgt ccccgccccc agcgcgcgct
    14401 tcctgccacg ttgcgcaggg gcgcgcggcc agactctgcg gcgcggggcc gaggggaggg
    14461 ccggaacctg ggagcgcctc ctcgccgccc ccgctggccg gcggatggac tcaacttgca
    14521 cgaacacgag ccaatggcaa gggccagttt tctgggcccc gagagccaat cagacgacga
    14581 ggcccggccg gcggcgggta aaacgactcc cccagaggaa ggggagggtg ggcggccgct
    14641 cgcgcgcgag ctactttcgc tgaccctccc tcccctcccc cgccccgcca gaggccgacc
    14701 gcgcccgcac gtccagctcg cctcacccca cctacctccc gccccaccca gtgggcagag
    14761 cgaggctgcc ggcggctgcg cactccggct gccgttaact gacaggcgcc ttacgccaac
    14821 caaaacacgc catttgtgtt ttcacacacg gcgggaggaa aagaagccaa tcagcgacga
    14881 gacgtcggcc ggaagcgctc ctccgctgcc cccccccccc cccgagccat ggccgcgtcc
    14941 ggtggagact tttccgctcc cttctccctc cccctccggt tgctgcaggg cggaccgcat
    15001 tcctgcccac cacccgcttg ccccttccag cgtcacgact cgtacccggc tgtctcacag
    15061 aacggctcca ccacgctcgg agggcctgcc gcggtggagc tccaattcgc cctatagtga
    15121 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
    15181 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
    15241 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggaaatt
    15301 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
    15361 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
    15421 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
    15481 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
    15541 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
    15601 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
    15661 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
    15721 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
    15781 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
    15841 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
    15901 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
    15961 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
    16021 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
    16081 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
    16141 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
    16201 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
    16261 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
    16321 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
    16381 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
    16441 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
    16501 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
    16561 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
    16621 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
    16681 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
    16741 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
    16801 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
    16861 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttc
//
