LOCUS       sequence-419891-       11196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contains human codon optimized sequence of EcSTH-LbNOX 
            which can be used to decrease NADPH/NADP ratio in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11196)
  AUTHORS   Yang R, Yang C, Ma L, Zhao Y, Guo Z, Niu J, Chu Q, Ma Y, Li B
  TITLE     Identification of purine biosynthesis as an NADH-sensing pathway to 
            mediate energy stress.
  JOURNAL   Nat Commun. 2022 Nov 17;13(1):7031. doi: 10.1038/s41467-022-34850-0.
  PUBMED    36396642
REFERENCE   2  (bases 1 to 11196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-11-17"; volume: "13(1):7031. doi"; pages: " 
            10.1038/s41467-022-34850-0"
COMMENT     Sequence Label: EcSTH-LbNOX
FEATURES             Location/Qualifiers
     source          1..11196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2206..2555
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    2214..2232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2250..2268
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2286..2304
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2322..2340
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2358..2376
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2394..2412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2430..2448
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2494..2518
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2622..2631
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3984..4049
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5420..5993
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5443..5995
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5587..5604)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5814..5833
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        6029..6528
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6064..6087)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6461..6480
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6549..7148
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6549..6568)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7045..7065
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7165..7753
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7218..7238)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7636..7647)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7960..8593
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8721..8737)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8721..8737)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8734..8756)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8745..8761
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8769..8775,8776..8793,8794..8799))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8814..8835
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8952..8969)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9123..9711)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9203..9222)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9882..10673,10674..10742))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10505..10524
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10743..10847)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10895..11029
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10932..10951)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10986..11005
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat
     2221 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac
     2281 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga
     2341 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat
     2401 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatgtcgag
     2461 gtaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc
     2521 ctggagcaat tccacaacac ttttgtctta tacttggatc catgccccac tcctacgact
     2581 acgacgccat cgtgatcggc tccggccccg gcggcgaggg cgccgccatg ggcctggtga
     2641 agcagggcgc ccgcgtggcc gtgatcgagc gctaccagaa cgtgggcggc ggctgcaccc
     2701 actggggcac catcccctcc aaggccctgc gccacgccgt gtcccgcatc atcgagttca
     2761 accagaaccc cctgtactcc gaccactccc gcctgctgcg ctcctccttc gccgacatcc
     2821 tgaaccacgc cgacaacgtg atcaaccagc agacccgcat gcgccagggc ttctacgagc
     2881 gcaaccactg cgagatcctg cagggcaacg cccgcttcgt ggacgagcac accctggccc
     2941 tggactgccc cgacggctcc gtggagaccc tgaccgccga gaagttcgtg atcgcctgcg
     3001 gctcccgccc ctaccacccc accgacgtgg acttcaccca cccccgcatc tacgactccg
     3061 actccatcct gtccatgcac cacgagcccc gccacgtgct gatctacggc gccggcgtga
     3121 tcggctgcga gtacgcctcc atcttccgcg gcatggacgt gaaggtggac ctgatcaaca
     3181 cccgcgaccg cctgctggcc ttcctggacc aggagatgtc cgactccctg tcctaccact
     3241 tctggaactc cggcgtggtg atccgccaca acgaggagta cgagaagatc gagggctgcg
     3301 acgacggcgt gatcatgcac ctgaagtccg gcaagaagct gaaggccgac tgcctgctgt
     3361 acgccaacgg ccgcaccggc aacaccgact ccctggccct gcagaacatc ggcctggaga
     3421 ccgactcccg cggccagctg aaggtgaact ccatgtacca gaccgcccag ccccacgtgt
     3481 acgccgtggg cgacgtgatc ggctacccct ccctggcctc cgccgcctac gaccagggcc
     3541 gcatcgccgc ccaggccctg gtgaagggcg aggccaccgc ccacctgatc gaggacatcc
     3601 ccaccggcat ctacaccatc cccgagatct cctccgtggg caagaccgag cagcagctga
     3661 ccgccatgaa ggtgccctac gaggtgggcc gcgcccagtt caagcacctg gcccgcgccc
     3721 agatcgtggg catgaacgtg ggcaccctga agatcctgtt ccaccgcgag accaaggaga
     3781 tcctgggcat ccactgcttc ggcgagcgcg ccgccgagat catccacatc ggccaggcca
     3841 tcatggagca gaagggcggc ggcaacacca tcgagtactt cgtgaacacc accttcaact
     3901 accccaccat ggccgaggcc taccgcgtgg ccgccctgaa cggcctgaac cgcctgttcg
     3961 gaggaggagg atcaggagga ggagactaca aagaccatga cggtgattat aaagatcatg
     4021 acatcgacta caaggatgac gatgacaaga tgaaggtcac cgtggtcgga tgcacccatg
     4081 ccggcacctt cgccatcaag caaatcctcg ctgagcaccc tgacgccgag gtcaccgtct
     4141 acgagaggaa cgatgtgatc tccttcctgt cctgtggcat cgccctctac ctgggcggaa
     4201 aagtggccga tccccaaggc ctcttctaca gctcccctga agaactgcag aagctgggcg
     4261 ctaatgtgca gatgaaccac aacgtgctgg ccatcgaccc tgaccaaaag accgtcacag
     4321 tcgaggacct caccaatcac gcccagacca ccgagtccta cgacaaactg gtgatgacct
     4381 ccggaagctg gcctatcgtg cccaaaatcc ccggcatcga cagcgatagg gtgaagctct
     4441 gcaagaattg ggcccacgcc caggctctga ttgaggacgc caaggaggcc aagaggatca
     4501 ccgtcatcgg cgccggatac atcggagccg aactggccga ggcctactcc acaacaggcc
     4561 acgacgtcac cctgattgac gccatggcta gggtcatgcc caagtacttc gatgccgact
     4621 tcaccgacgt catcgaacag gactacaggg accatggcgt gcaactcgct ctgggcgaga
     4681 cagtggagag cttcaccgac agcgccaccg gcctcacaat caagacagac aagaactcct
     4741 atgagaccga cctggccatc ctctgcattg gctttaggcc caacacagac ctgctgaaag
     4801 gcaaagtgga catggcccct aacggcgcca tcattaccga cgactacatg aggtccagca
     4861 accctgatat tttcgctgct ggcgactccg ccgccgtcca ttacaacccc acacaccaaa
     4921 acgcctacat tcccctcgct accaacgccg tcaggcaggg aatcctcgtc ggaaagaacc
     4981 tcgtcaagcc cacagtgaag tacatgggaa cccagtccag ctccggactg gccctctatg
     5041 acaggacaat tgtctccaca ggcctcacac tggccgccgc caagcaacaa ggcctcaatg
     5101 ccgagcaggt catcgtggag gacaactata ggcccgagtt catgccttcc accgagcccg
     5161 tcctcatgag cctggtcttc gaccccgata cacacagaat cctgggaggc gccctgatgt
     5221 ccaaatacga cgtgtcccag agcgctaata ccctgtccgt ctgcatccag aacgagaaca
     5281 ccatcgatga cctggccatg gtggacatgc tgttccagcc caatttcgac aggcccttca
     5341 actacctgaa cattctcgcc caggctgccc aagctaaagt ggcccaatcc gtcaacgctt
     5401 aagcggccgc cccggccggc ccctctccct cccccccccc taacgttact ggccgaagcc
     5461 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     5521 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     5581 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     5641 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     5701 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     5761 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     5821 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     5881 ctgatctggg gcctcggtac acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     5941 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc
     6001 cccgggacgc gtcatatgga attctaccgg gtaggggagg cgcttttccc aaggcagtct
     6061 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
     6121 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
     6181 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
     6241 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
     6301 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
     6361 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
     6421 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
     6481 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca
     6541 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     6601 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     6661 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     6721 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     6781 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     6841 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     6901 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     6961 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     7021 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     7081 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     7141 gtgcctgacg ggcgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg
     7201 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     7261 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     7321 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7381 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     7441 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7501 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     7561 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     7621 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     7681 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     7741 gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca
     7801 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     7861 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     7921 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc
     7981 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     8041 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     8101 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     8161 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     8221 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     8281 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     8341 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     8401 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8461 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8521 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8581 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     8641 aaatgaatat cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc
     8701 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     8761 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     8821 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     8881 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     8941 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     9001 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     9061 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     9121 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     9181 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     9241 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     9301 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     9361 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     9421 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     9481 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     9541 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     9601 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     9661 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     9721 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     9781 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     9841 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     9901 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9961 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    10021 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    10081 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    10141 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    10201 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    10261 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    10321 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    10381 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    10441 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    10501 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    10561 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    10621 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    10681 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    10741 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    10801 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    10861 aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg
    10921 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    10981 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
    11041 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
    11101 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
    11161 ttgtatttgt taaatatgta ctacaaactt agtagt
//
