LOCUS       sequence-419902-        4410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express hcoRBFOX1N-PCP-RBFOX1C(190-397).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Liu Z, Jillette N, Robson P, Cheng AW
  TITLE     Simultaneous multifunctional transcriptome engineering by CRISPR RNA
            scaffold.
  JOURNAL   Nucleic Acids Res. 2023 Aug 11;51(14):e77. doi: 10.1093/nar/gkad547.
  PUBMED    37395412
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2023-08-11"; volume: "51(14):e77. doi"; pages: "
            10.1093/nar/gkad547"
COMMENT     Sequence Label: pmax-hcoRBFOX1N-PCP-RBFOX1C
FEATURES             Location/Qualifiers
     source          1..4410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          813..945
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     protein_bind    960..984
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2439..2463)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2516..2557
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(2583..2602)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    2612..2693
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2637..2656
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(2891..3479)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2971..2990)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3560..4354)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3560..4354)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3671..3690)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4281..4300
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagg
      721 tcgtttagtg aaccgtcaga tcactagtag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctc gactgatcac aggtaagtat caaggttaca agacaggttt
      841 aaggaggcca atagaaactg ggcttgtcga gacagagaag attcttgcgt ttctgatagg
      901 cacctattgg tcttactgac atccactttg cctttctctc cacagggaaa aaacaattga
      961 caagtttgta caaaaaagca ggctccgaat tcaccggtgc cgccaccatg aattgcgagc
     1021 gggaacagct caggggcaat caagaagcgg cagctgcacc tgacacaatg gcacagccgt
     1081 atgcgagtgc tcaatttgcg ccaccccaga atggcatacc cgcagaatac acagcgccgc
     1141 atcctcaccc cgcccctgaa tacactgggc aaactaccgt tcctgaacac acgcttaacc
     1201 tctatcctcc tgctcagact cactcagaac agagtccagc agatacaagt gcgcagacag
     1261 tctctggcac tgcgacccag accgatgacg cagctcctac tgatggccag ccacaaactc
     1321 aaccctctga aaacactgaa aataaaagcc aacccaaggg cggtggaggc tccgggcgcg
     1381 ccatgggttc caaaaccatc gttctttcgg tcggcgaggc tactcgcact ctgactgaga
     1441 tccagtccac cgcagaccgt cagatcttcg aagagaaggt cgggcctctg gtgggtcggc
     1501 tgcgcctcac ggcttcgctc cgtcaaaacg gagccaagac cgcgtatcgc gtcaacctaa
     1561 aactggatca ggcggacgtc gttgattccg gacttccgaa agtgcgctac actcaggtat
     1621 ggtcgcacga cgtgacaatc gttgcgaata gcaccgaggc ctcgcgcaaa tcgttgtacg
     1681 atttgaccaa gtccctcgtc gcgacctcgc aggtcgaaga tcttgtcgtc aaccttgtgc
     1741 cgctgggccg tggtggcgga gggactagtg gaggtggatc cggaagtggg ccggccaatg
     1801 ccacggctag ggtcatgaca aacaagaaga cggttaatcc gtataccaac gggtggaaac
     1861 ttaatcccgt tgtcggcgct gtctattctc ccgagttcta tgctggtaca gtcttgctgt
     1921 gccaagccaa tcaagagggg tcttcaatgt acagtgctcc gagcagcttg gtttacacaa
     1981 gcgcgatgcc aggatttcct taccctgcgg caacggccgc ggctgcgtat cggggtgccc
     2041 accttcgcgg gcggggccga acagtttaca acacgttcag ggccgctgct ccacctcccc
     2101 cgattcccgc gtacggaggt gttgtctacc aagacgggtt ttatggagcc gacatctacg
     2161 gtggttacgc cgcatatcgg tatgcacaac caacccccgc gaccgcggca gcgtacagcg
     2221 actcctacgg acgcgtgtac gctgctgatc cttaccacca cgccctggcc cccgccccaa
     2281 cgtatggtgt cggtgctatg aatgcgttcg cgcccctgac cgatgcaaaa accaggtcac
     2341 atgccgacga cgttggattg gtattgagct ctttgcaagc atccatttac cgggggggat
     2401 acaaccgctt cgcgccttat tgattaatta agaattcgac ccagctttct tgtacaaagt
     2461 ggttgatatc cagcacagtg gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa
     2521 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggttagc aattgttttt
     2581 tcgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     2641 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     2701 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     2761 agtaaaacct ctacaaatgt ggtacttaag agggggagac caaagggcga gacgttaagg
     2821 cctcacgtga catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2881 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2941 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3001 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttacggg atacctgtcc gcctttctcc
     3061 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3121 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3181 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3241 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3301 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     3361 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3421 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3481 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3541 ggattttggt catgccgtct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct
     3601 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
     3661 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
     3721 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
     3781 aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgctcgcc ttgagcctgg
     3841 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
     3901 gaccggcttc catccgagta cgtgctctct cgatgcgatg tttcgcttgg tggtcgaatg
     3961 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
     4021 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
     4081 gccagtccct tcccgcttca gtgacaacgt cgagtacagc tgcgcaagga acgcccgtcg
     4141 tggccagcca cgatagccgc gctgcctcgt cttgcagttc attcagggca ccggacaggt
     4201 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
     4261 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
     4321 gagaacctgc gtgcaatcca tcttgttcaa tcataatatt attgaagcat ttatcagggt
     4381 tcgtctcgtc ccggtctcct cccatgcatg
//
