LOCUS       sequence-419910-       12341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12341)
  AUTHORS   Naviaux RK, Costanzi E, Haas M, Verma IM
  TITLE     The pCL vector system: rapid production of helper-free, high-titer, 
            recombinant retroviruses.
  JOURNAL   J Virol. 1996 Aug . 70(8):5701-5.
  PUBMED    8764092
REFERENCE   2  (bases 1 to 12341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1996 Aug . 70(8):5701-5."
COMMENT     Sequence Label: pCL-Eco
FEATURES             Location/Qualifiers
     source          1..12341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(6..74,75..866)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(224..243)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1037..1625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1526..1545
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1779..1796
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      1861..1995
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2453..2587
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2477..2496)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2531..2550
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2859..2879)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          3009..3074
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(join(5724..9323,9324..9326,9327..10940))
                     /codon_start=1
                     /gene="gag-pol"
                     /product="Moloney murine leukemia virus Gag-Pol 
                     polyprotein"
                     /label=gag-pol
                     /note="contains a read-through stop codon"
                     /translation="MGQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPT
                     FNVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPK
                     PPPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGAKPKPQVLSDSGGPLIDLLTEDPP
                     PYRDPRPPPSDRDGNGGEATPAGEAPDPSPMASRLRGRREPPVADSTTSQAFPLRAGGN
                     GQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGTLLTGE
                     EKQRVLLEARKAVRGDDGRPTQLPNEVDAAFPLERPDWDYTTQAGRNHLVHYRQLLLAG
                     LQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETNVSMSFIW
                     QSAPDIGRKLERLEDLKNKTLGDLVREAEKIFNKRETPEEREERIRRETEEKEERRRTE
                     DEQKEKERDRRRHREMSKLLATVVSGQKQDRQGGERRRSQLDRDQCAYCKEKGHWAKDC
                     PKKPRGPRGPRPQTSLLTLDD*GGQGQEPPPEPRITLKVGGQPVTFLVDTGAQHSVLTQ
                     NPGPLSDKSAWVQGATGGKRYRWTTDRKVHLATGKVTHSFLHVPDCPYPLLGRDLLTKL
                     KAQIHFEGSGAQVMGPMGQPLQVLTLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAE
                     TGGMGLAVRQAPLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPW
                     NTPLLPVKKPGTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKD
                     AFFCLRLHPTSQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFDEALHRDLADFRI
                     QHPDLILLQYVDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGY
                     LLKEGQRWLTEARKETVMGQPTPKTPRQLREFLGTAGFCRLWIPGFAEMAAPLYPLTKT
                     GTLFNWGPDQQKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWR
                     RPVAYLSKKLDPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPP
                     DRWLSNARMTHYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRP
                     DLTDQPLPDADHTWYTDGSSLLQEGQRKAGAAVTTETEVIWAKALPAGTSAQRAELIAL
                     TQALKMAEGKKLNVYTDSRYAFATAHIHGEIYRRRGLLTSEGKEIKNKDEILALLKALF
                     LPKRLSIIHCPGHQKGHSAEARGNRMADQAARKAAITETPDTSTLLIENSSPYTSEHFH
                     YTVTDIKDLTKLGAIYDKTKKYWVYQGKPVMPDQFTFELLDFLHQLTHLSFSKMKALLE
                     RSHSPYYMLNRDRTLKNITETCKACAQVNASKSAVKQGTRVRGHRPGTHWEIDFTEIKP
                     GLYGYKYLLVFIDTFSGWIEAFPTKKETAKVVTKKLLEEIFPRFGMPQVLGTDNGPAFV
                     SKVSQTVADLLGIDWKLHCAYRPQSSGQVERMNRTIKETLTKLTLATGSRDWVLLLPLA
                     LYRARNTPGPHGLTPYEILYGAPPPLVNFPDPDMTRVTNSPSLQAHLQALYLVQHEVWR
                     PLAAAYQEQLDRPVVPHPYRVGDTVWVRRHQTKNLEPRWKGPYTVLLTTPTALKVDGIA
                     AWIHAAHVKAADPGGGPSSRLTWRVQRSQNPLKIRLTREAP"
     misc_feature    5787..6161
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(5833..5849)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     complement(10537..10553)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(10569..10591)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     promoter        complement(11249..11452)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(11278..11298)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        11453..11832
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(11977..11996)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12156..12174)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(12242..12341,1..5)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
       61 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      121 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      181 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      241 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      301 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      361 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      421 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      481 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      541 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      601 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      661 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      721 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      781 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      841 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
      901 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
      961 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1021 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1081 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1141 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     1201 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1261 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1321 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1381 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1441 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1501 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1561 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1621 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1681 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1741 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1801 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1861 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
     1921 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     1981 agttaggggc gggatgggcg gagtaagacc cgcacccgtt cccaaactgc ttattaaagg
     2041 gggtatggag gtgctggacc ttgtgacagg gccagacagt gtgacagaaa tagaagcagt
     2101 cctgttttga caagttgcct ctggaagcct ctacaatgcc tctcttcttt ttctccagag
     2161 taagcggagg ccaggggccc ccggcctctg cttaatacta aaaaaaacag ctgttgtcat
     2221 agtaatgatt gggtggaaac attctaggcc tgggtggaga ggctttttgc ttcctcttgc
     2281 aaaaccacac tgccctctgg agggcagttg cctagcaact aattaaaaga ggatgtcgca
     2341 cggccagctg cggtcagtta gtcacttcct gcttaactga cttgacattt tctattttaa
     2401 gagtcgggag gaaaattact gtgttggagg ccctccgcca tcttctgaag ctgatccaga
     2461 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     2521 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     2581 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     2641 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atctctagtc
     2701 aaggcactat acatcaaata tttttccata attttcttgt atagcagtgc agctttttcc
     2761 tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc atgactcaaa
     2821 aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt cttttttgga
     2881 ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat ttcttctgag
     2941 caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc agttccatag
     3001 gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta tacacttaaa
     3061 aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa ttatgtcaca
     3121 ccacagaagt aaggttcctt cacaaagatc ctctagctac ctagctagat acactccgct
     3181 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     3241 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     3301 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc cagcttacct
     3361 cccggtggtg ggtcggtggt ccctgggcag gggtctcccg atcccggacg agcccccaaa
     3421 tgaaagaccc ccgctgacgg gtagtcaatc actcagagga gaccctccca aggaacagcg
     3481 agaccacaag tcggatgcaa ctgcaagagg gtttattgga tacacgggta cccgggcgac
     3541 tcagtcaatc ggaggactgg cgccccgagt gaggggttgt gggctctttt attgagctcg
     3601 gggagcagaa gcgcgcgaac agaagcgaga agcgaactga ttggttagtt caaataaggc
     3661 acagggtcat ttcaggtcct tggggcaccc tggaaacatc tgatggttct ctagcttgcc
     3721 aaacctacag gtggggtctt tcattccccc ctttttctgg agactaaata aaatctttta
     3781 ttttatctat ggctcgtact ctataggctt cagctggtga tattgttgag tcaaaactag
     3841 agcctggacc actgatatcc tgtctttaac aaattggact aatcgattaa gaatgcaggg
     3901 tccgaagagc aaaatcatta ggagtacaat gaggggtccc ataatggtag atatcaaggt
     3961 ggtaaaccaa ggggatctgt taaacagtcc ctcaaaccat ccttgagttg actcaaacag
     4021 tttctgtctc tgattaagcc tctctctcaa tttggccatg ctgtctctca ctagtcctgt
     4081 gtggtccgca tagaagcaac attcttcttt tagagcagca cacagccctc cttcttttag
     4141 aaataacaag tctaggcccc ttcgattctg taggacaact tcagacaggg aagtgagaga
     4201 cttttctagg ttagagattg atttttcaac ctccctgaga tcatcctgta ctgcggcttg
     4261 gagctgctgg aattgctgag tggccattag agcagtagtc cctgttccta ttccagcggc
     4321 aattcccccc atggttagtc cacccaataa tagggccagg gttaacgaca ccggttctct
     4381 tttgtgtcgg ttggatctct caaacaggcc gtaaacatag ctgggggaat gataggtgac
     4441 tcttggccag agttcgacaa gaacacaata atcagtggta aggttcagta tggtggtgga
     4501 gatgcatgga gtaagcccgg tactacaagc ccacatggta cctgtagggg caactagata
     4561 ataggaccct cgactgcttg tctgggtggt attacatagg gcctgatgtg ttttgggaac
     4621 tgctcctatg cagagtccct gtccggtcac ttcggacagg gtcaacttgt gttgggaggc
     4681 cacggagcag ttggctggag cagaggtatg gttggagtag gtacccagga cggcaacccc
     4741 ttcgtagtag gggggtcccg ctactagaca caaccagcac tcttgggttt tgtcaggact
     4801 ggtgaggttg agggcttggt aggctccgtc tactaagttt agcagcctat tttccgttcc
     4861 cgccggtgga agttgggtag gggagagagg agtcccactg ggtggtttgg tgactgaagg
     4921 cgacttaaca ggtttgggct tggagagtgg ctgttggtct gccagaacgg ggtttggccc
     4981 tattgggacg cggggtccta gattttggta tctgagtcgg atcccaaatg taagccctgg
     5041 atcttgtccg gagacataca aacgtaagcc ccagtaatgt cctgtggtcc aggaagtaac
     5101 ccgtctcccg gcgtctgtaa accgaataac taaggggttg caccacttat tatctttgca
     5161 tacctggaca gcctggtcag aggtgagatt gttgtttact gtgatgaaat cccatgatga
     5221 ggagggcttc cagtaagctc taccggttgt ctcacagccc caataggcac agtagaagga
     5281 gtctggaccc ccacatgact tggattctcg ggggcggtgg ggcccggggc aaacataaaa
     5341 tccctcattt gatttatgag ttgtctggtc tagcttgagt ctgttccagg cagtgttgca
     5401 ccgaggggtg agggaggtta aaggttcttc gcagtctctg gaacagcctg ggctgctgcc
     5461 ccctgagcaa caagggggcc ccgggggaga agaaaaaggg gattgatatt ctagccccca
     5521 ataagatggt ccatggtggg ctaacataca taaatctggg gtaaggtcag gccaccaggt
     5581 ccacagaggg tggttgccag aagttgccca taccgtctcc cgatctccat tggttacctc
     5641 ccaggtgata ttatagactt gatgaggact ggagccgggc gaagcagtac tgacccctct
     5701 gagcatcaga agaattaagg ggattagggg gcctcgcggg ttaaccttat ttttaagggg
     5761 ttttgagagc gttgaacgcg ccatgtcagt ctagaggatg gtccaccccc ggggtcggca
     5821 gccttcacgt gggcggcgtg tatccaagct gcgatgccgt ctactttgag ggcggtgggg
     5881 gtggtcagca ggactgtgta aggtcctttc cagcgaggtt ctaggttctt agtctggtgt
     5941 cggcggaccc acactgtgtc gccgactcgg taagggtgag gtaccaccgg tcggtccagt
     6001 tgttcttggt aggctgccgc cagaggtctc cagacttcgt gctggactaa gtagagagcc
     6061 tgtaagtgag cttggagaga ggggctgtta gtaactcttg tcatgtcagg gtcagggaag
     6121 tttacaaggg gcgggggtgc cccatataag atctcatatg gggtgaggcc atgggggccc
     6181 ggcgtgttgc gggctcggta cagggctaag gggagtagga gcacccagtc tctagagcca
     6241 gttgcaagcg ttaatttagt taaagtctcc ttgatggttc tattcattct ttctacctgg
     6301 cctgagcttt ggggtctgta tgcacaatgt aatttccaat caatccccaa cagatcggcc
     6361 actgtctgac tcaccttgga gacgaaggca ggcccattgt cagttcccaa tacctgaggc
     6421 atgccgaacc tggggaagat ctcctctagt agcttcttgg ttacgacctt ggcggtttct
     6481 ttcttggttg ggaaggcttc tatccagcca gaaaaggtat ctataaaaac tagaagatat
     6541 ttatagccat acaatccggg ctttatctcg gtgaaatcga tctcccaatg agtgccgggc
     6601 cgatgcccgc ggaccctagt tccctgttta acggcagact tgctggcgtt gacttgtgca
     6661 caagctttgc aggtctcagt gatatttttg agtgttcgat cccggttcag catgtagtag
     6721 ggactgtggc ttctctctag gagagccttc atttttgaga agctgaggtg agtcagctga
     6781 tgaagaaagt ctaataattc aaaagtaaac tggtcaggca tcacaggttt tccttggtag
     6841 acccaatact tctttgtttt atcataaatg gcccccaact tggttaggtc ctttatatca
     6901 gtcactgtgt aatgaaaatg ttctgaggtg tagggtgatg aattttctat gaggagggta
     6961 gaggtgtctg gagtctctgt gatggctgcc tttcgggccg cttggtcagc catccggttg
     7021 cctctagcct cggcgctgtg tcccttttga tgtcctggac aatggattat gctaagtctt
     7081 ttgggcagaa agagggcttt tagtagggcc aagatctcgt ctttattttt gatctctttg
     7141 ccttctgatg tgagcaaccc acgccttctg tatatttctc catggatatg ggcagtagca
     7201 aaagcataac ggctatcagt ataaacattt agcttcttac cttctgccat ctttagggcc
     7261 tgggtgagtg ctatcagttc agcccgctga gcggatgtcc cggctggcag ggctttagcc
     7321 cagattacct cggtctcggt ggtcaccgca gctcccgcct tacgctgtcc ctcttgtaag
     7381 agactgcttc catccgtgta ccaggtgtgg tcggcgtctg ggagcggctg gtccgttagg
     7441 tcgggtcggg ttccgtgggc ttcggccagg atatcaaggc agttgtgttg cagcccttcc
     7501 tcaggcagtg ggagcagcgt agccgggttc agggctacca ccggtccgaa ctggacccgg
     7561 tccgtgtcca aaagcaaggc ctgatagtga gtcatccggg cgttggaaag ccagcggtcg
     7621 gggggttgtt tgactagtgc ctctactgca tggggggcca gaatgactag tggctgtccc
     7681 atggttagct tgcctgcatc ctttgtcagt acggcaatgg ctgctaccat ccgtaggcaa
     7741 gggggccacc cagctgctac tgggtctagc tttttggaca ggtaggccac cggccgacgc
     7801 caaggtccca gtttttgcgt taggacacct ttggcgtagc cctgcttctc gtcgacaaag
     7861 agttcaaagg gcttagtcaa atctggcaac cccagggctg gggcagttag aagagcttgc
     7921 ttgatttctt gataggcctt ttgttggtct gggccccaat taaacagagt ccccgttttg
     7981 gtgagagggt acaagggggc tgccatttct gcaaacccag ggatccagag gcgacagaag
     8041 cctgccgtcc ctaggaactc ccttagttgt cgaggggtct tcggagtagg ctgccccatc
     8101 acagtctctt ttctggcctc agtcagccat ctctgaccct cttttagaag ataccccaga
     8161 tacttgacct gtttctggca aatttgggct ttcttggccg aggcccgata cccgaggttc
     8221 cctagggttt gtaacagggc ccgagtacct tgttggcagt ctagctcaga agtggcggcc
     8281 agcagtaagt catccacgta ctgtagcagg atcaagtctg ggtgctggat ccggaagtct
     8341 gctaggtctc tgtgcagtgc ctcatcaaac agggtgggac tgtttttgaa accctgtggg
     8401 agtctggtcc aggtcaattg tcctgagatt cccatctctg gatctctcca ctcaaaggcg
     8461 aagagaggct gactggtggg gtggagtctc aggcagaaaa aggcatcctt taaatcaagc
     8521 acagtgtacc actggtggga cggtgggagc ccgctcaaga ggttgtaagg gttgggcacg
     8581 gtggggtgga tgtcttccac ccgcttgttg acttctctca gatcctggac aggcctataa
     8641 tcattagtcc ctggtttctt aacgggtagc aggggcgtgt tccaggggga ctggcagggt
     8701 accagtattc cctggtccaa cagtctctgt atgtggggct tgatccccag tctggcttct
     8761 tgtgacatgg ggtattgttt tatggacacg ggggtagagg ttgctttcag aggtatgatc
     8821 agaggagctt ggcgaactgc cagtcccatg cccccggttt ccgcccaggc ctgaggaaaa
     8881 tcagacagcc atgtggaccc tagagaaaca tctggctctt ttgaggtctc atgtagccga
     8941 tactcatctt ctatatttag ggtcaacact tgcaggggct gccccattgg tcccataacc
     9001 tgagctcctg atccctcaaa gtggatttgg gcttttagtt tagtcagcaa atctcttcct
     9061 aacagaggat agggacagtc tggtacatgg aggaaagagt gggtgacctt accggtagct
     9121 agatgtactt tgcgatccgt ggtccagcga taccgctttc ctccagtagc cccttggacc
     9181 caggcagact tatcacttag gggtccagga ttttgggtca gcacggagtg ttgggcccca
     9241 gtatctacca ggaaggtgac gggttgcccc ccgactttga gggttatcct gggttcaggg
     9301 gggggctcct gaccctgacc tccctagtca tctagggtca ggagggaggt ctggggtctt
     9361 ggtccccgag gtcctcgtgg tttcttggga caatctttag cccagtgccc cttttctttg
     9421 cagtaggcac actggtcgcg atcgagttgg gacctccttc gttctcctcc ctgtctatcc
     9481 tgtttctgtc cactaacgac agtggccaat agcttgctca tctctctatg tctcctacga
     9541 tctctttctt tctctttctg ctcatcctct gtcctacggc gttcttcttt ttcctctgtt
     9601 tctctcctga tacgttcctc tctttcttcc ggggtttctc gtttattaaa gatcttttct
     9661 gcctctctaa ccaaatctcc aagcgtcttg ttttttaaat cttctaacct ctctaacttt
     9721 ctcccaatgt ctggggcaga ctgccaaatg aaagacatag acacattagt ttcttgccct
     9781 gggtcctcag ggtcataagg agtgtacctg cgataggctt ccttaagtct ctctaggaag
     9841 gccgagggag actcattggg cccttgtgtt attcctttta ccttggccaa attggtgggg
     9901 cttctgcccg cgttttggag acccgctagg agcaactggc gatagtggac taggtggttc
     9961 ctacctgcct gggtggtgta atcccagtct gggcgctcga ggggaaaagc ggcatcgact
    10021 tcattgggca gttgagtggg gcgcccatca tcgccccgca ccgcctttct agcctctaag
    10081 agcacccgtt gtttttcttc tccggtcagc agagtcccca acagctgctg acagtcgtcc
    10141 caggtgggct gatgggtgat gaggacagac tcgatcagag ctgtcagttt acctggatct
    10201 tcagaaaaag aagggttatt atttttccag ttgtaaaggt cagaagagga gaacggccag
    10261 tattgaagct gtccgtttcc tcctgcgcgg agggggaatg cctgcgaggt agtggagtcg
    10321 gccacagggg gctcccgtct cccacgtagg cgagatgcca ttggggaggg gtccggtgcc
    10381 tctcccgcag gggtcgcttc tccaccattt ccgtccctgt cggaaggggg tggtcttggg
    10441 tccctataag gcggggggtc ttctgtaagt aggtcgatga gcggcccccc actgtcagaa
    10501 agaacttgag gtttaggttt ggcgcctaga gaaggagtga gggctggata aagggaggat
    10561 cgaggcgggg tcgaacgagg aggttcaagg gggagagacg gggcggatgg aggaagagga
    10621 ggcggaggct tagggtgtac aaagggcttg acccagggag gggggtcaaa agccaaggct
    10681 tcccaggtca cgatgtaggg gacctggtct gggtgtccat gcgggccagg tgaaaagacc
    10741 ttgatcttaa cctgggtgat gaggtctcgg ttaaaggtgc cgtctcgcgg ccatccgacg
    10801 ttaaaggttg gccattctgc agagcagaag gtaacccaac gtctcttctt gacatctacc
    10861 gactggttgt gagcgatccg ctcgacatct ttccagtgac ctaaggtcaa acttaaggga
    10921 gtggtaacag tctggcccat attttcagac aaatacagaa acacagtcag acagagacaa
    10981 cacagaacga tgcccaagct tggccagctt acctcccggt ggtgggtcgg tggtccctgg
    11041 gcaggggtct cccgatcccg gacgagcccc caaatgaaag acccccgctg acgggtagtc
    11101 aatcactcag aggagaccct cccaaggaac agcgagacca caagtcggat gcaactgcaa
    11161 gagggtttat tggatacacg ggtacccggg cgactcagtc aatcggagga ctggcgcgcc
    11221 gagtgagggg ttgtgggctc ttttattgag ctctgcttat atagacctcc caccgtacac
    11281 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
    11341 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
    11401 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa
    11461 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
    11521 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
    11581 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
    11641 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
    11701 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
    11761 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca
    11821 ataatcaatg tcaacgcgct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
    11881 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct
    11941 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
    12001 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
    12061 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
    12121 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
    12181 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
    12241 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
    12301 acaataaccc tgataaatgc ttcaataata ttgaaaaagg a
//
