LOCUS       sequence-419939-       25258 bp    DNA     circular UNA 15-DEC-2025
DEFINITION  A plasmid containing a 15,249 bp fragment of the P. tricornutum 
            chloroplast genome. The fragment is flanked by SapI recognition 
            sites, allowing it to be released from the cloning vector pSAP1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 25258)
  AUTHORS   Walker EJL, Pampuch M, Chang N, Cochrane RR, Karas BJ
  TITLE     Design and assembly of the 117-kb Phaeodactylum tricornutum 
            chloroplast genome.
  JOURNAL   Plant Physiol. 2023 Dec 19:kiad670. doi: 10.1093/plphys/kiad670.
  PUBMED    38114089
REFERENCE   2  (bases 1 to 25258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 Dec 19:kiad670. doi: 10.1093/plphys/kiad670."
COMMENT     Sequence Label: pCHAP2
FEATURES             Location/Qualifiers
     source          1..25258
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      3329..3943
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      3420..3951
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      4019..4238
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             4329..5084
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    5087..5337
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     misc_feature    5499..6003
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        6057..6204
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             6205..6879
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSEASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     CDS             7275..8450
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             8450..9421
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNKRDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHTGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    10485..10883
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(10901..10934)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     11002..11021
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11214..11236
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11228..11245
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11229..11245
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11252..11271
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11252..11270
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    11273..11329
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(11372..11388)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11372..11388)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11385..11407)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11396..11412
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11420..11426,11427..11444,11445..11450))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11465..11486
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(11706..12365)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     12280..12299
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(12366..12468)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 tttacaatct aatttaataa ttggtttaac actgttgaag cgggcaacgc gattcgaacg
       61 cgcgacatca accttggcaa ggttgcgctc taccactgag ctatgcccgc taacaaagtt
      121 ttaaacgaat tacttacctt acacacataa tacagaaggc ggataatatt tgtcaatact
      181 ttctttttaa aataatttag aaattatagt taaactataa tttctaaatt attaaagtga
      241 tgatgcaaca ccatcagctg ccgcaattac aagaaccagt gcaacccaag cctgaaaacc
      301 ttttgtaaaa tcagatttgg aattttccca ttcaccggga gtcgctaatg ctactggaac
      361 cgtcacaatt aaacctaatg aaattaaaac caataaagcc gttaaagcag ttatcataag
      421 tatttttaaa aaatttttat attgatgatt cgaataaaat aaatatagta ctaaagatta
      481 cctttcttta atgctaataa aacaattact gctgggcctg ctaacatgat agtccctgtc
      541 gctattagtt gtcctagaac ttgccaatta atcatttttt aaatccttat ttgtcaattt
      601 ttaataaaat tttaagtaat aaaataaccg ataatgttat tttctattct actttaaaac
      661 taataaagta aaaatattat tttaattaat attgtagttg gtatagtttt aaaaaataca
      721 attttttttt aagaaatact ttaatatttt tgcaagttca tgtaatatat tatgtaaggg
      781 ttgctaactc aatggtagag tactcggctt ttaaccgaat agttctgggt tcgagtccca
      841 ggcaacccaa ttttgaactt aacaaaatat ttaatcgatt tattacaaaa aatttcttat
      901 atcttgctta aaattaatta ttaaatattt gattattctt tactcaacat atattgagta
      961 aagaataaaa gtgattatta atcaatagga cgcatcgata acacaggttc attagcacga
     1021 ttaatacctt tctcgaaacc agcagccgcc gcacgagcac gacctgaatg ccaccagtga
     1081 ccaactaaga aaaagaatcc taagaagaaa tgagaacaac ataaccatga acgaggagaa
     1141 acataattaa ctgagttaat ttctgtcgct acaccaccta cggaattaag tgaacctaat
     1201 ggagcgtgtg tcatatattc agctgcacga cgttcttgcc aaggttgaat atcattttta
     1261 attttgttga tatctaaacc atttggacca cgtaatggtt caacccacgg tgcacgtaaa
     1321 tcccaaaaac gcattgtttc accaccaaag atgatttcac cactaggtga acgcattaag
     1381 tatttaccta aaccagttgg accctgtgct gatgaaacat tagcacctaa acgttgatct
     1441 cgaactaaaa acgtgaatgc ttgtgcttgt gaagcctcag gacctgttgg gccatataat
     1501 tcgctcggat aagctgtatt gttataccaa gaatataatg acgctgttaa acccattaat
     1561 gaaattgcag ctaaactata tgataaataa gcttcacctg accatacaaa agcacgacga
     1621 gcccatgcaa atggttttgt gaaaatatgc caaataccac cagtaataca taatacacca
     1681 acccatacat gaccaccaat aacatcttcc atgttattaa ctgatacaac ccaaccgtca
     1741 ccaccaaatg gtgaacggaa tacataacca aaaattacaa ttgggtttaa tgtaggtgtt
     1801 gtaattaaac gaacatcgcc accacctggt gcccaagtat cataaacacc accaatgtac
     1861 attgctttag caactaataa aaaagcacca ctacctaaaa gacataaatg aatccctaga
     1921 atagttgtca tcttgttttt atcacgccag tcataaccaa agaatgggaa tgattcttct
     1981 aatgtatctg gtccaagtaa tgagtggtaa ataccgccaa aacctaaaac agctgatgaa
     2041 attaaatgaa ttacacctac agcaaaatat gggacagtgt tagtaatttc accgccaggg
     2101 ccaattccgt aacctaaagt tgctaaatgt ggcatacaaa tgaaaccttg ttcatataaa
     2161 ggtttctcag gtacgaagtg agaaacttcg aataaaatca ttgctccagc ccaaaatacc
     2221 attaaaccag catgtgcaac gtgtgcaccc aataacttac ctgaaacatt aattaaccga
     2281 gcatttccac tccaccaagc gaatccggta gattcgatgt cgcggcctgc tatactacta
     2341 ttaaagggcg tttccacgtg gcaatacctc ctcagggaat acaaagtttt catgtggttg
     2401 atcttgtgca gccatccaag cacgaatacc ttcgtttaat aaaatatttt ttgtatagaa
     2461 cgtttcaaat tcaggatctt cagcagcacg taattcttgt gatacaaaat cataagcacg
     2521 taaatttaat gctaaaccaa cgataccaat agcacttgtc cataaacctg tcactggtac
     2581 gaatagcatg aaaaaatgta accaacgttt atttgaaaaa gctacaccga aaatttgtga
     2641 ccaaaaacga ttcgctgtaa ccattgaata cgtttcttct gattgtgttg gcgtgaacgc
     2701 acggaatgta tttgcagcat caccatcttc aaataatgtg ttttctactg ttgcaccatg
     2761 aatagcacaa agtaaagcac cacctaaaat tccagcaaca cccatcatat ggaatgggtt
     2821 taatgtccag ttatggaaac cttgtaagaa tagtaaaaaa cggaagattg cagcaacacc
     2881 aaaactaggc gcaaagaacc aacttgcttg acctaatggg tataatagga aaactgaaac
     2941 aaatactgca attggacctg aaaacgcaat tgcattataa ggtcgaatac ctactaaacg
     3001 tgcaatttca aattgacgta aacagaatcc gattaaaccg aatgaaccgt gtaaagcgac
     3061 aaatgtccaa agaccaccga tttgacacca acgtgtgaaa tcaccttgcg cttcagggcc
     3121 ccataaaagc aataacgaat gacccatact gttagctgga ctcgaaacag cagcagttaa
     3181 gaagttacat ccttcaagat atgaacttgc taaaccatga gtgtaccacg atgtaacaaa
     3241 tgttgtacca gtcatccaac caccaacagc taaatacgct gtcgggaata ataataaacc
     3301 tgaccaacca acgaaaacaa tgaagagcct ggttgccctc gccgctgggc tggcggccgt
     3361 ctatggccct gcaaacgcgc cagaaacgcc gtcgaagccg tgtgcgagac accgcggccg
     3421 gccgccggcg ttgtggatac ctcgcggaaa acttggccct cactgacaga tgaggggcgg
     3481 acgttgacac ttgaggggcc gactcacccg gcgcggcgtt gacagatgag gggcaggctc
     3541 gatttcggcc ggagacgtgg agctggccag cctcgcaaat cggcgaaaac gcctgatttt
     3601 acgcgagttt cccacagatg atgtggacaa gcctggggat aagtgccctg cggtattgac
     3661 acttgagggg cgcgactact gacagatgag gggcgcgatc cttgacactt gaggggcaga
     3721 gtgctgacag atgaggggcg cacctattga catttgaggg gctgtccaca ggcagaaaat
     3781 ccagcatttg caagggtttc cgcccgtttt tcggccaccg ctaacctgtc ttttaacctg
     3841 cttttaaacc aatatttata aaccttgttt ttaaccaggg ctgcgccctg tgcgcgtgac
     3901 cgcgcacgcc gaaggggggt gccccccctt ctcgaaccct cccggtcgag tgagcgagga
     3961 agcaccaggg aacagcactt atatattctg cttacacacg atgcctgaaa aaacttccct
     4021 tggggttatc cacttatcca cggggatatt tttataatta ttttttttat agtttttaga
     4081 tcttcttttt tagagcgcct tgtaggcctt tatccatgct ggttctagag aaggtgttgt
     4141 gacaaattgc cctttcagtg tgacaaatca ccctcaaatg acagtcctgt ctgtgacaaa
     4201 ttgcccttaa ccctgtgaca aattgccctc agaagaagct gttttttcac aaagttatcc
     4261 ctgcttattg actctttttt atttagtgtg acaatctaaa aacttgtcac acttcacatg
     4321 gatctgtcat ggcggaaaca gcggttatca atcacaagaa acgtaaaaat agcccgcgaa
     4381 tcgtccagtc aaacgacctc actgaggcgg catatagtct ctcccgggat caaaaacgta
     4441 tgctgtatct gttcgttgac cagatcagaa aatctgatgg caccctacag gaacatgacg
     4501 gtatctgcga gatccatgtt gctaaatatg ctgaaatatt cggattgacc tctgcggaag
     4561 ccagtaagga tatacggcag gcattgaaga gtttcgcggg gaaggaagtg gttttttatc
     4621 gccctgaaga ggatgccggc gatgaaaaag gctatgaatc ttttccttgg tttatcaaac
     4681 gtgcgcacag tccatccaga gggctttaca gtgtacatat caacccatat ctcattccct
     4741 tctttatcgg gttacagaac cggtttacgc agtttcggct tagtgaaaca aaagaaatca
     4801 ccaatccgta tgccatgcgt ttatacgaat ccctgtgtca gtatcgtaag ccggatggct
     4861 caggcatagt ctctctgaaa atcgactgga tcatagagcg ttaccagctg cctcaaagtt
     4921 accagcgtat gcctgacttc cgccgccgct tcctgcaggt ctgtgttaat gagatcaaca
     4981 gcagaactcc aatgcgcctc tcatacattg agaaaaagaa aggccgccag acgactcata
     5041 tcgtattttc cttccgcgat atcacttcca tgacgacagg atagtctgag ggttatctgt
     5101 cacagatttg agggtggttc gtcacatttg ttctgaccta ctgagggtaa tttgtcacag
     5161 ttttgctgtt tccttcagcc tgcatggatt ttctcatact ttttgaactg taatttttaa
     5221 ggaagccaaa tttgagggca gtttgtcaca gttgatttcc ttctctttcc cttcgtcatg
     5281 tgacctgata tcgggggtta gttcgtcatc attgatgagg gttgattatc acagtttatt
     5341 actctgaatt ggctatccgc gtgtgtacct ctacctggag tttttcccac ggtggatatt
     5401 tcttcttgcg ctgagcgtaa gagctatctg acagaacagt tcttctttgc ttcctcgcca
     5461 gttcgctcgc tatgctcggt tacacggctg cggcgagcat cacgtgctat aaaaataatt
     5521 ataatttaaa ttttttaata taaatatata aattaaaaat agaaagtaaa aaaagaaatt
     5581 aaagaaaaaa tagtttttgt tttccgaaga tgtaaaagac tctaggggga tcgccaacaa
     5641 atactacctt ttaccttgct ctacctgctc tcaggtatta atgccgaatt gtttcatctt
     5701 gtctgtgtag aagaccacac acgaaaatcc tgtgatttta cattttactt atcgttaatc
     5761 gaatgtatat ctatttaatc tgcttttctt gtctaataaa tatatatgta aagtacgctt
     5821 tttgttgaaa ttttttaaac ctttgtttat tttttttttc ttcattccgt aactcttcta
     5881 ccttctttat ttactttcta aaatccaaat acaaaacata aaaataaata aacacagagt
     5941 aaattcccaa attattccat cattaaaaga tacgaggcgc gtgtaagtta caggcaagcg
     6001 atccagatgg cagtagtgga agatattctt tattgaaaaa tagcttgtca ccttacgtac
     6061 aatcttgatc cggagctttt ctttttttgc cgattaagaa ttaattcggt cgaaaaaaga
     6121 aaaggagagg gccaagaggg agggcattgg tgactattga gcacgtgagt atacgtgatt
     6181 aagcacacaa aggcagcttg gagtatgtct gttattaatt tcacaggtag ttctggtcca
     6241 ttggtgaaag tttgcggctt gcagagcaca gaggccgcag aatgtgctct agattccgat
     6301 gctgacttgc tgggtattat atgtgtgccc aatagaaaga gaacaattga cccggttatt
     6361 gcaaggaaaa tttcaagtct tgtaaaagca tataaaaata gttcaggcac tccgaaatac
     6421 ttggttggcg tgtttcgtaa tcaacctaag gaggatgttt tggctctggt caatgattac
     6481 ggcattgata tcgtccaact gcatggagat gagtcgtggc aagaatacca agagttcctc
     6541 ggtttgccag ttattaaaag actcgtattt ccaaaagact gcaacatact actcagtgaa
     6601 gcttcacaga aacctcattc gtttattccc ttgtttgatt cagaagcagg tgggacaggt
     6661 gaacttttgg attggaactc gatttctgac tgggttggaa ggcaagagag ccccgaaagc
     6721 ttacatttta tgttagctgg tggactgacg ccagaaaatg ttggtgatgc gcttagatta
     6781 aatggcgtta ttggtgttga tgtaagcgga ggtgtggaga caaatggtgt aaaagactct
     6841 aacaaaatag caaatttcgt caaaaatgct aagaaatagg ttattactga gtagtattta
     6901 tttaagtatt gtttgtgcac ttgccgatct atgcggtgtg aaataccgca cagatgcgta
     6961 aggagaaaat accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     7021 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     7081 aatcatttta cacaggagtc tggacttgac gctagtgata ataagtgact gaggtatgtg
     7141 cgcttcttat ctccttttgt agtgttgctc ttattttaaa caactttgcg gttttttgat
     7201 gactttgcga ttttgttgtt gctttgcagt aaattgcaag atttaataaa aaaacgcaaa
     7261 gcaatgatta aaggatgttc agaatgaaac tcatggaaac acttaaccag tgcataaacg
     7321 ctggtcatga aatgacgaag gctatcgcca ttgcacagtt taatgatgac agcccggaag
     7381 cgaggaaaat aacccggcgc tggagaatag gtgaagcagc ggatttagtt ggggtttctt
     7441 ctcaggctat cagagatgcc gagaaagcag ggcgactacc gcacccggat atggaaattc
     7501 gaggacgggt tgagcaacgt gttggttata caattgaaca aattaatcat atgcgtgatg
     7561 tgtttggtac gcgattgcga cgtgctgaag acgtatttcc accggtgatc ggggttgctg
     7621 cccataaagg tggcgtttac aaaacctcag tttctgttca tcttgctcag gatctggctc
     7681 tgaaggggct acgtgttttg ctcgtggaag gtaacgaccc ccagggaaca gcctcaatgt
     7741 atcacggatg ggtaccagat cttcatattc atgcagaaga cactctcctg cctttctatc
     7801 ttggggaaaa ggacgatgtc acttatgcaa taaagcccac ttgctggccg gggcttgaca
     7861 ttattccttc ctgtctggct ctgcaccgta ttgaaactga gttaatgggc aaatttgatg
     7921 aaggtaaact gcccaccgat ccacacctga tgctccgact ggccattgaa actgttgctc
     7981 atgactatga tgtcatagtt attgacagcg cgcctaacct gggtatcggc acgattaatg
     8041 tcgtatgtgc tgctgatgtg ctgattgttc ccacgcctgc tgagttgttt gactacacct
     8101 ccgcactgca gtttttcgat atgcttcgtg atctgctcaa gaacgttgat cttaaagggt
     8161 tcgagcctga tgtacgtatt ttgcttacca aatacagcaa tagcaatggc tctcagtccc
     8221 cgtggatgga ggagcaaatt cgggatgcct ggggaagcat ggttctaaaa aatgttgtac
     8281 gtgaaacgga tgaagttggt aaaggtcaga tccggatgag aactgttttt gaacaggcca
     8341 ttgatcaacg ctcatcaact ggtgcctgga gaaatgctct ttctatttgg gaacctgtct
     8401 gcaatgaaat tttcgatcgt ctgattaaac cacgctggga gattagataa tgaagcgtgc
     8461 gcctgttatt ccaaaacata cgctcaatac tcaaccggtt gaagatactt cgttatcgac
     8521 accagctgcc ccgatggtgg attcgttaat tgcgcgcgta ggagtaatgg ctcgcggtaa
     8581 tgccattact ttgcctgtat gtggtcggga tgtgaagttt actcttgaag tgctccgggg
     8641 tgatagtgtt gagaagacct ctcgggtatg gtcaggtaat aaacgtgacc aggagctgct
     8701 tactgaggac gcactggatg atctcatccc ttcttttcta ctgactggtc aacagacacc
     8761 ggcgttcggt cgaagagtat ctggtgtcat agaaattgcc gatgggagtc gccgtcgtaa
     8821 agctgctgca cttaccgaaa gtgattatcg tgttctggtt ggcgagctgg atgatgagca
     8881 gatggctgca ttatccagat tgggtaacga ttatcgccca acaagtgctt atgaacgtgg
     8941 tcagcgttat gcaagccgat tgcagaatga atttgctgga aatatttctg cgctggctga
     9001 tgcggaaaat atttcacgta agattattac ccgctgtatc aacaccgcca aattgcctaa
     9061 atcagttgtt gctctttttt ctcacaccgg tgaactatct gcccggtcag gtgatgcact
     9121 tcaaaaagcc tttacagata aagaggaatt acttaagcag caggcatcta accttcatga
     9181 gcagaaaaaa gctggggtga tatttgaagc tgaagaagtt atcactcttt taacttctgt
     9241 gcttaaaacg tcatctgcat caagaactag tttaagctca cgacatcagt ttgctcctgg
     9301 agcgacagta ttgtataagg gcgataaaat ggtgcttaac ctggacaggt ctcgtgttcc
     9361 aactgagtgt atagagaaaa ttgaggccat tcttaaggaa cttgaaaagc cagcaccctg
     9421 atgcgacctc gttttagtct acgtttatct gtctttactt aatgtccttt gttacaggcc
     9481 agaaagcata actggcctga atattctctc tgggcccact gttccacttg tatcgtcggt
     9541 ctgataatca gactgggacc acggtcccac tcgtatcgtc ggtctgatta ttagtctggg
     9601 accacggtcc cactcgtatc gtcggtctga ttattagtct gggaccacgg tcccactcgt
     9661 atcgtcggtc tgataatcag actgggacca cggtcccact cgtatcgtcg gtctgattat
     9721 tagtctggga ccatggtccc actcgtatcg tcggtctgat tattagtctg ggaccacggt
     9781 ccaactcgta tcgtcggtct gattattagt ctggaaccac ggtcccactc gtatcgtcgg
     9841 tctgattatt agtctgggac cacggtccca ctcgtatcgt cggtctgatt attagtctgg
     9901 gaccacggtc caactcgtat cgtcggtctg ataatcagac tgggaccacg gtcccactcg
     9961 tatcgtcggt ctgattatta gtctgggacc atggtcccac tcgtatcgtc ggtctgatta
    10021 ttagtctggg accacggtcc cactcgtatc gtcggtctga ttattagtct ggaaccacgg
    10081 tcccactcgt atcgtcggtc tgattattag tctgggacca cggtcccact cgtatcgtcg
    10141 gtctgattat tagtctggga ccacgatccc actcgtgttg tcggtctgat tatcggtctg
    10201 ggaccacggt cccacttgta ttgtcgatca gactatcagc gtgagactac gattccatca
    10261 atgcctgtca agggcaagta ttgacatgtc gtcgtaacct gtagaacgga gtaacctcgg
    10321 tgtgcggttg tatgcctgct gtggattgct gctgtgtcct gcttatccac aacattttgc
    10381 gcacggttat gtggacaaaa tacctggtta cccaggccgt gccggcacgt taaccgggct
    10441 gcatccgatg caagtgtgtc gctgtcgacg agctcgcgag ctcggacatg aggttgcccc
    10501 gtattcagtg tcgctgattt gtattgtctg aagttgtttt tacgttaagt tgatgcagat
    10561 caattaatac gatacctgcg tcataattga ttatttgacg tggtttgatg gcctccacgc
    10621 acgttgtgat atgtagatga taatcattat cactttacgg gtcctttccg gtgatccgac
    10681 aggttacggg gcggcgacct cgcgggtttt cgctatttat gaaaattttc cggtttaagg
    10741 cgtttccgtt cttcttcgtc ataacttaat gtttttattt aaaataccct ctgaaaagaa
    10801 aggaaacgac aggtgctgaa agcgagcttt ttggcctctg tcgtttcctt tctctgtttt
    10861 tgtccgtgga atgaacaatg gaagtccgag ctcatcgcta ataacttcgt atagcataca
    10921 ttatacgaag ttatattcga tgcggccgca aggggttcgc gtcagcgggt gttggcgggt
    10981 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
    11041 ggtgtgaaat accacacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
    11101 tcagctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    11161 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgcaagggt tttcccagtc
    11221 acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattcga
    11281 gctcggtacc cagggatcct ctagagtcga cctgcaggca tgcaagcttg agtattctat
    11341 agtctcacct aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
    11401 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
    11461 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttctagtcgg
    11521 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgaacccctt gcggccgccc
    11581 gggccgtcga ccaattctca tgtttgacag cttatcatcg aatttctgcc attcatccgc
    11641 ttattatcac ttattcaggc gtagcaacca ggcgtttaag ggcaccaata actgccttaa
    11701 aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
    11761 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
    11821 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
    11881 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
    11941 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
    12001 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
    12061 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
    12121 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
    12181 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
    12241 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
    12301 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
    12361 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
    12421 gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt
    12481 cgccaaaagt tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct
    12541 gcgaagtgat cttccgtcac aggtatttat tcgcgataag ctcatggagc ggcgtaaccg
    12601 tcgcacagga aggacagaga aagcgcggat ctgggaagtg acggacagaa cggtcaggac
    12661 ctggattggg gaggcggttg ccgccgctgc tgctgacggt gtgacgttct ctgttccggt
    12721 cacaccacat acgttccgcc attcctatgc gatgcacatg ctgtatgccg gtataccgct
    12781 gaaagttctg caaagcctga tgggacataa gtccatcagt tcaacggaag tctacacgaa
    12841 ggtttttgcg ctggatgtgg ctgcccggca ccgggtgcag tttgcgatgc cggagtctga
    12901 tgcggttgcg atgctgaaac aattatcctg agaataaatg ccttggcctt tatatggaaa
    12961 tgtggaactg agtggatatg ctgtttttgt ctgttaaaca gagaagctgg ctgttatcca
    13021 ctgagaagcg aacgaaacag tcgggaaaat ctcccattat cgtagagatc cgcattatta
    13081 atctcaggag cctgtgtagc gtttatagga agtagtgttc tgtcatgatg cctgcaagcg
    13141 gtaacgaaaa cgatttgaat atgccttcag gaacaataga aatcttcgtg cggtgttacg
    13201 ttgaagtgga gcggattatg tcagcaatgg acagaacaac ctaatgaaca cagaaccatg
    13261 atgtggtctg tccttttaca gccagtagtg ctcgccgcag tcgagcgaca gggcgaagcc
    13321 cgctcttcaa catccaagcc cagactgata aattattcat accaaatggg ttataaccgt
    13381 taattagtgg tgatgaattt aaccataaat agtcacgtag ccagcccatg atgtaatttg
    13441 atgactcatc aaactgccct gggttaccac cccaaattgt catgtgtttc caatgccaat
    13501 agaatgtcac ccacccaatt gtgtttaaca tccagaacat tgctaaataa aatgcatccc
    13561 aagcagaaat atcacaagta ccaccacgac ctggaccatc acaagggaaa ctataaccaa
    13621 aatctttttt atctggcatt agttttgatc cacgcgcatc taatgcacct ttcacaagaa
    13681 ttaatgtagt agtatgtaaa cctaaagcaa ttgcatgatg aactaaaaag tcaccagggc
    13741 caatttttaa aaataaatca tttttattat tattaatcgc ttctaaccaa cccggtaacc
    13801 aaatttgatt accagctaca gtagctggac tggttgaaga agataataaa gtgttaaatt
    13861 cgtaaactgc tttaccagaa gctgcttgaa tatactcagc aaataatggt tcaaataaaa
    13921 tttgtttttc tggttgacca aaagcaacta cagtatcatt gtggatatat aaacctaacg
    13981 tatggaagcc taagaataat gacgcccaac ttaagtgaga aataatagct tctttatgct
    14041 ctagcatgcg tgctaaaaca ttattcttat ttagctctgg gtcataatca cgaacaaaga
    14101 aaattgcgcc gtgcgcaaag gcgccgacca ttaagaatcc agcaatatat tgatgatgcg
    14161 tatacaatgc tgcttcagta gtaaaatctt tagataagaa agcataagga gtaattgcat
    14221 acatatgttg cgcggttaat gatgtagcaa cgcctaaaca tgctaatgct aaacctagtt
    14281 gcatatgtaa cgaatttgta attgtttcaa acaaaccagt atgaccagca cctaaacggc
    14341 ctccaggagg acggtgagcg tctaaaattt ctttcatatt atggccgata ccaaagtttg
    14401 ttcgatacat atgtcctgca acaataaaaa caaccgcaat cgctaattgg tggtgcgcaa
    14461 tatcacttaa ccataaagat tgtgtttgcg ggtggaaacc acctaaaaat gttaaaatag
    14521 ctgtaccagc accttctgca gttccataaa catgatttgc tgaatctgga tttgctgcgt
    14581 acacagtcca gtcaccactg taaaaaggtg ttaaaccagc cgggtgaggt ggagttgtta
    14641 aaaagttctc ccaaccaaca tgtacacccc gtgatgcagg aatagccacg tgaacaatat
    14701 gtccagtcca agctagcgaa ctgacgccta ataaacctga taagtgatga tttagacgag
    14761 attcattatt tttgaaccag gctaaacttg gtcgaaattt tggttgtaaa tgtaaccaac
    14821 cagcaaataa taaaactgat gctaaaatta ataaaccaac tgaacctaag tataattctt
    14881 gattcgttct aaaaccaata gtataccacc attggtatac gcctgaaaaa gctatattta
    14941 ccggataagt gttaccttta ctaaatgctt taattgctga ttcaccaaaa tgaggatccc
    15001 aaattgcatg tgcaattggt ttcactttta atggattact tacccatttt tcaaaatttc
    15061 cttgccaagc gacatgaaat agatttcctg cagtccacaa gaaaataacg gctaaatgac
    15121 caaaatgcga agcgaaaatt ttttggtata aattttcttc agtcatacca tcatgtgctt
    15181 ctaaatcatg agcagtagcg ataccatacc aaattcttcg cgttgctgga tcttgtgcaa
    15241 gggcttggct aaactttgga aatttagttg ccataattgt tagatgcctt tttatataaa
    15301 ttttagttat gaataaaaac cgtatttagt ttattttagt taactgattg aaacagcacg
    15361 agctaagaag aacgcccaag tagttccaat accacccaat aaataatgag ctaaaccaac
    15421 tgcacgaccc tgagtaatgc ttaatgcacg aggttggatt gcaggagcaa aatttaattt
    15481 attatgagcc caaacaattg attcaattaa ttcttgccaa taaccacgac cactgaacaa
    15541 gaacattaaa ctaaacgccc aaataaagtg agcacctaag aaaattagac cataagctga
    15601 cgcagctgag ccatatgatt gaattacttg cgaagcttgt gaccataaaa aatcacgtaa
    15661 ccaaccatta attgtaattg caccttttgc gaaattacca cctgtaatat gtgaaatact
    15721 accatcaggt gaaatagtac cccagacatc tgattgcatt ttccaactga aatggaaaat
    15781 tacaactgaa atagagttat acatccagaa taagcctaaa aatacatgat cccagcttga
    15841 actttgacac gtaccaccac gacctgggcc atcacatggg aaccggaaac ctaaattagc
    15901 tttatctgga attaattttg aactacgcgc atataaaacg ccttttaata aaattaaaac
    15961 cgttacatga attgtaaatg catgaatatg atgaaccata aaatctgcag ttcctaattg
    16021 aataggcatc attgcgattt ttccaccaac ttctacaact tcaccaccaa aagcataact
    16081 tgttgtcgcc aaagcattcg gagctgttgt acctggtgct aataaatgaa tattttgaat
    16141 ccactgagca aagattggtt gtagttgaat cgctttatct gaaaacatat cttgaggacg
    16201 acctaaagca cgcattgtat cattatgaat atagaagcca aacgcatgac aacctaagaa
    16261 aatacaaacc caatttaaat gcgcaataat cgagtcacga tgacgtaata cacgatctag
    16321 caaattatta taattatttg ctggtgtata atcccgaacc ataaatattg cagcatgggc
    16381 tgctccacca gtaacacaga atcctccaat ccacatatga tgtgtaaata aagaaagttg
    16441 tgttgcataa tcagtagcaa tataaggata cggaggcatt gcgtacatgt ggtgtgccac
    16501 aataatactt aatgaaccca tcattgctag attaattgct aattgagcat gccaagatgt
    16561 tgttaaaatt tcgtataagc ctttatgacc ttcacctgta aatggtcctt tatgagcttc
    16621 taaaatttct ttcatactgt gaccgatacc ccaattagta cggtacatat ggccagcaaa
    16681 aataaataag acagctaatg ctaaatgatg atgagcaata tcacttaacc ataaaccacc
    16741 ggttacaggg tttaaaccac ctttgaatgt taaaaagtct gagtattctc cccaatgacc
    16801 actgaaaaat ggtgctaacc cttttgaaaa acttgggtac agttgactca ttaattcacg
    16861 attaattaaa aattcgtgag gtaatggaat ttcttgtggt gctacacccg catctagtag
    16921 tttattaatc ggtaaagcaa catgaatttg gtgaccagac caagataaac accctaaacc
    16981 taataaacca gctaaatgat gattcatcat cgactcagcg ttttggaacc actctaattt
    17041 cggtgctgct ttatgataat gaaaccagcc agcaaacaac ataatcgcag acattgctaa
    17101 accaccaatt gcaatccaat ataattcaac ttcactggta attccttctg cacgccacat
    17161 ttggaaccaa ccagacgttg tctgaatacc ttggaaatta ccaccaacat caccatttag
    17221 aatttcttga ccaacgattg gccaaacgac ttgagaactt tgtttaatat taactggatc
    17281 agttaaccaa gccgaataat tagagaaata tgctccatgg aagtgcatac cactgatcca
    17341 taaaaatatt actgataact gtccaaagtg cgcactaaaa attttacgcg aaacttcttc
    17401 taaagaactt gtctgactat caaagtcatg agcatctgca tgtaaattcc aaatccaagt
    17461 agttgttttt ggacctttag ctaaagttcg agagaaatga ccaggttgtg cccattttgc
    17521 gaaactagtt tcgactgcgt ctttctcaac aaaaatttgc acgttttttg agcgacggtc
    17581 ggttgagctt attgccatta attttctcct tgttgggaga cgaaaattta tgaaactctc
    17641 ataaaaaata aagaatagac acgaattaac tatctgttaa ttatgagttt tcaattttac
    17701 attatacatt tatttaaaaa attttaggta tttatttttc atttcaaaat taaaaataaa
    17761 ataatgaatt aaaacccata ttgatatgag ttttaattaa tcttagaatt ggattaaaga
    17821 ttaaaaatat aaccctaata aatcagggaa aaaacgatta atctcaataa taaaacctgc
    17881 tgtaaatgtt aaccaaattg tcaataaaac aggagccgtt gataagtatt tttgaaaatt
    17941 ttccataaaa gcttagtttg aatagttaat aaataattaa tgaagaagtt taaatgttaa
    18001 taattaacga ggtgaaactg tcacttcatc ttttggcgct attaattcac cactaatcaa
    18061 ttcttgccat gctgaaattg gccaaatgta acccgttgtc atgattttaa tggccaatgg
    18121 gacattaata ataatttcgc tttccgctgg attcttagtc gtgctaacag cacgaacata
    18181 cttacgccca acccaaccaa tccaacctga aatatagata aatccaaaac ctggaagaat
    18241 aaattcagca gcatgactcc aacgtccatc tgcaactaaa tgtggtagtc cctcagtacc
    18301 gcacaataaa tctgaacgac tatatttgtc aaatctggct tttgttctat caatttgttg
    18361 ttgcaacgct aaagatggag gtgaatcagc ttgatattga ctcattcttt gttcaagttt
    18421 tttgacacta gcatttaaac gttttgtaaa agctggagat tcactacatt tcgttaaccc
    18481 accaatatca gcaactgctt gacccggtgt gaaagttaat aatgctaaaa aaagtaaagt
    18541 tattaaatta aaacgtttca taagaaatta aattcataat tgtaaatttg gtaaaaaatt
    18601 ttagaaaggt aaaattacta ctatttacat aatagtatag attaaaaaat attatttatt
    18661 tgaaaaaaaa attaaaaaaa aattaaaaaa atcaaatctt cattagacac tataaaaagt
    18721 ttttagaagt attcacaaaa tgtaaaatac tctaaaaact aaaggtatta aaaattactc
    18781 gaaatcttta cgatttggat ttcttgatgg atcacttgaa agaagtccaa agataaataa
    18841 cgaaacaaaa aagataatcg ttgtgtaaac taaaattttt aaagttaaca tttaattttt
    18901 cttaaaaatt atttttgata atattaatta tactaaaata aaactattat gaattattta
    18961 attcaaaaaa aattatctaa cttgctctta agctttctca tttgaacgat catagctcaa
    19021 aagaaatgga aaaacacgta aaactgttat ttcagttttt ttaggagttt gaggttttga
    19081 actcaaggac gacttttctc tgagtgaaat atagccttca atcaaaatgt aatcattaac
    19141 tttataataa ttaatgacat cattggctaa atttccccaa aacgttagat taactatgcg
    19201 actaccacga acttgtggta attgaacacg aaattttaca attgtaatat tattttttaa
    19261 agaacgttgt tttggtgttt caataatttt gacaattccc ccaatataat ttgtatcacc
    19321 catttttaat taaatgatta aattcttttg tttttgaaca tcttcaaaat taatatcacg
    19381 caaacgtttt aataaacgaa taaaatattc taagaaataa tcatccaatt gaattatttc
    19441 tgacgaatca attttaaata gcttatataa atcaaatgtt gattttgaaa aattattttc
    19501 agtatttaaa atttccgcaa atgctaggcg atcgctgata ttctgacccc attcaaaaaa
    19561 tccaaaaaat ctactaacaa attttagaag aaataaagga attttttgta cttttgcttc
    19621 ttgtccagcc aattgttcac ataaattaat aatttctgat gaaacccaac cacgtgagcc
    19681 actaagaaaa aatgtttgat taatcgtttc ttgaatttgt aatgaacgta ggcaaaattt
    19741 tgctatatct tgcgtatcca tatacgaaat ataggtattt tcattagtta cccaaatagg
    19801 taaattttct aaaaccggaa ttgcgtattg ttcaatcaat ccttgataaa atccagtcaa
    19861 tcgaaaaatt gtatatggta tttcagattg ttttaattta atctctattc catttttaac
    19921 tttcattaac ggaatatttt caaactgttc tacattttga gcagaaaaaa agataaaacg
    19981 ctttatattg gccgcctttg ctgcttcaat taaatataac ttaccttccc aatcaacttt
    20041 ttttagagag ttttgctcat ttgctcgact tgttgacgca tcaataatgg cagtaattcc
    20101 ttttagacaa ggcgcaattg tctcaggacg agctaaatca ccataaacta gctcaacacc
    20161 ccattctttt agaaagctag cttttcgaaa atttcgaact agacatcgta cttgataacc
    20221 tttggttagg gcttgtaaaa caacttgtcg ccctaatgtt ccagttcctc caataataag
    20281 taaactcata aatcgattaa tttcttaatt attaaatact gtactttgta aaatatctgc
    20341 tgcttcaaga tttactaatt ctttttttgt taatacttgg ccgcggctat caaaaattct
    20401 attcgcttga cgcatacgtg ctcgatctaa ggcattttta acacttctag cattagcaaa
    20461 taacggcttc tctttgcgtt tttgaatata ttgtgtcaaa gctgattcag catctggagt
    20521 taattgatat tgttgttctt ctaacatcaa ttttgcaatt tttaataatt cttcaactgt
    20581 ataatctgga aagtcaatgt ggtttgcgat acgtgatgat aaacctggat ttgattcgta
    20641 gaatttatcc attggttctt tataacctgc taatatcaca actaaatcat cacgttgatt
    20701 ctccattact tgtaataaaa tttcaatagc ttctgagccg tagtcacgtt cattatctgg
    20761 tttgtataaa taataagctt cgtcaataaa taaaactcca cccattgctt ttttaagaac
    20821 ttctttagtt tttggagccg tatgtccaat atattgacct actaaatcat cccgagttac
    20881 cgttaataaa tgaccttttt taatataccc taactgatat aaaatttcgg ccatttttaa
    20941 accaacggtt gttttaccag ttccaggact accagtaaaa gacatatgta ggccaggatg
    21001 ggctgaggtg attcctaagt ttcgtcttaa tttatctatt aataataaag cagcaacttc
    21061 tcgaatccga gtttttactg gtgctaaacc gactaattct tcatctaaca agcttaatgt
    21121 ctttgcaatt ttcgtttttc catattcttc ttgtaaacgt acttgagttg aatccatatt
    21181 gttttacttt ttaaaaataa atttgtagcc aaagtagtcg tatgaaatct gttaatagat
    21241 ttcatacgac tactttaata tatacttaat ttgttataaa gctagggata ctagatagac
    21301 aaataaatgc aaagcctgca cttccacatg ccccaacaaa gaacccacct ttaaattggt
    21361 cccaagcctt ctttgtttgt aaatcatttt cattagatga ttgcgtcttt tcttggccaa
    21421 acgcagctac accgtaaatc gttagaccta aagttaaaat tacaatcaaa ccaactgttg
    21481 aaagaaaacc agctagcaat ccaatctcag aatcacgcaa tggccctaat ttaacaaagg
    21541 ggcccattaa aaagtaacca tgtgctaaac caatttctaa gccacgtaat aatggcgtta
    21601 aaccgaaacg ataggctggt aaattttgta gaattgctcg tgttactgca gatgatgtta
    21661 ttggcgttgc taaatgaccc acaaacggat catcattata gggtttaatg aaattagcca
    21721 taaaattaat ttaaaaatta gtttaattta agaataaaaa tcttctctaa aaaaatagac
    21781 agcaacaaaa atttgctgaa agttttttcc gaccaaaatt tttatataga ttactatata
    21841 atatatacta atatacagaa aaatttttat aaacttcatt ttgaaaaaat aagaaagatt
    21901 ttatgacagt tgctatcgat tactttttat tagtaggatt ctgcttcgca gttacatctg
    21961 gtttatggat aggattaaaa tcaattaaac taatttaatt tctaatttag ctacaccaaa
    22021 tgcaaaacga aattcgtcaa gataaaatta tagggtcacg acggtttagt aattatttct
    22081 gggcattctt tttacttgtc ggaggcttag gatttttatt agctgggata tctagttact
    22141 ttaaagttaa tttactacct tttacaaata cgacggaact ggtttttatt ccacaaggac
    22201 tagttatgat gttctacgga gcattaagtt taggaattag tatctataca ttgcttacaa
    22261 ttattttaga tattggaagc gggtataatg agtacaatag gatagaaaat cttgtgaaag
    22321 ttgtccggaa aggatttcca ggaaaaaatc gtgagatcct tttaacatat tctttatcaa
    22381 atgttcgagc gattgggatt aagatcacag aaggtttaaa tccaacacgc agcatttatt
    22441 tatgtttaaa agatgagcgt aatattcctt taacaccagt tcaagagcca actgctattt
    22501 ctaatcttga agaagaagca acggatttag caaaattttt ggatttaaga ctagaaaatt
    22561 tataagattt tattgctttt cggcccgtat aattctataa tgaggttatg cgggcatagt
    22621 ttagtggtaa aaccttagcc ttccaagcta atgatggggg ttcgattccc cctgcccgct
    22681 ttacataact acaccatatt aagattaaac ttagactgag caacaatctt ttttttatag
    22741 gagaatatct atatgtctgg tggatctacg ggtgaacgtc cgttttcgga tattattact
    22801 agtgtacgtt attggattat tcatagtatc actattccat ctctttttgt atcaggatgg
    22861 ttattcgtta gtactggttt agcatatgac gtatttggaa ctccacgccc aaatgaatat
    22921 ttcacacaag atcgtcaaca aattccatta gtaaacgatc gatttagtgc gaaacaagaa
    22981 ttagaagatt taactaaagg tttataattg aggtaaaaaa atggcaaaaa atattaatca
    23041 acctgtagct tatcctattt ttactttccg ttggttagca attcatggat tagcaatccc
    23101 aacagtattc ttcttaggtg gtattacagc aatgcaattc attcaacgat aaattcatat
    23161 acaatatata gacacgaggt aattttttat gacaggtcca aatccaaata agcaagcagt
    23221 agaattgaat agaacttctc tttattgggg acttttatta atttttgttt tagcagttct
    23281 attctcaagt tatttcttta attaatatta atatcatagg agcttttcaa aatggcaaat
    23341 actggtagaa tacctttatg gcttgtaggt ttagtaggtg gcttagcagt aattacgatg
    23401 cttggtttat ttatctatgg ttcatattct ggtttaggtt catcattata atatactatc
    23461 tgttaatatc cattaaacgt attttaatgg atattagcaa aaacagatgt taaataattt
    23521 gttatcaatt tttgaactgt tttttcaatc ttttcataaa ttttttcatt actcgaccta
    23581 atctattctc taacttccat ctaccatttg ttccgcgtcc aaaccaaccg taccacaact
    23641 ttggtaaaaa tcgttttaag tttttttctt tttcattagg atcttgccac gatgtctggc
    23701 ctccgctata aagactattt tttggtcttg gtccaatatg aagttctgta ttttcgacaa
    23761 aaaatggcat ataaaaatat tgaccccaaa tagcatgaaa tagaccaaaa ataataaaac
    23821 caatatgagc taaaacgaca catgcaataa ttccatttaa aacattaatt tgtaaaatta
    23881 agttgggatc aatataatta gcatatgact ctgtttgtgg aattaataca aatgtttgaa
    23941 aatagtatac acgataaata aaatagataa aaatttgttc aaccatacct attataagta
    24001 aaaatgtcca atgccatcta actaaatatg ggcaatatcg ttttcctatt cgagttataa
    24061 ccgcgtatgc tgcaactcca gaaagttgtg tccaaaattt actacaaatt tcatagattt
    24121 ttggtatgat cttattataa attttataat agagaggaag aatatttgat ttatttggtt
    24181 cttgcaagtt tgatacaatc attgaaattg gatcaatata atatcgaagt ccagtctcag
    24241 gatctatgtt agtaacacct tttgtaagag cataatatat aatttgccct ggattatacc
    24301 aatgtacatt ttcgcctaat tttaaatcag ttaaaaccat ttgcctttgt aatgagcgca
    24361 attggacggc atccattccc aatttatgta aaaatggaag cttcaataat acgcttcgat
    24421 acattgaaat taaatcgatt agatgtcgat accacaaata accagcaaaa agtccaatac
    24481 atgtaatata aaatgaagtc tttaaattgt atcgtaatgt aagaataaca aaacggacaa
    24541 aaagaatgaa aaaaagaaca ttctcaatat cagctaaagt tagcccatcc tgaatttttt
    24601 cccataaacc ctctacgatt aatcgaagag tagttaatga gacatctgca gaaggttcaa
    24661 aattagaaag attaaaatcg tatccatttg ttgctgcttg tcgaatttct cgttttgcag
    24721 gatcttccat tccaaaccaa tttaagactg tttcttgatc aatgttgatt aaaagaaata
    24781 aaagtctaaa aaaatatagc ataaattagt tctactaatc cattaattta aaagtttaaa
    24841 aagttaaata ttttattccc caaacatact gaatttttgt ataaagatca aggttttata
    24901 aataattcac tgacttaatt caccgtatac aagttaacta gtctaagtaa tattctatac
    24961 tagacaaaaa aataatttga ggaaaaattt tggaccaaac tctcctgtaa gaaaaaagtt
    25021 agtacgaatc tcaaatcaag aaaataatta ctttatgatg atctaattaa attatgaaac
    25081 attatttgat ataactatac tatttcaacg gtacatattt attataaaaa gtttaaaaag
    25141 ttgttaaatt gtatatttat atatatataa attaccccca agggaattcg aatccctgtc
    25201 tgctccgtga aagggagctg tcctaggcct ctagacgatg ggggcaaacg accaaaaa
//
