LOCUS       sequence-419955-       10135 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A piggyBac vector containing CMV-PARP1-del.p119K120S-eGFP-IRES-Neo 
            cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10135)
  AUTHORS   Krastev DB, Li S, Sun Y, Wicks AJ, Hoslett G, Weekes D, Badder LM, 
            Knight EG, Marlow R, Pardo MC, Yu L, Talele TT, Bartek J, Choudhary 
            JS, Pommier Y, Pettitt SJ, Tutt ANJ, Ramadan K, Lord CJ
  TITLE     The ubiquitin-dependent ATPase p97 removes cytotoxic trapped PARP1 
            from chromatin.
  JOURNAL   Nat Cell Biol. 2022 Jan;24(1):62-73. doi: 
            10.1038/s41556-021-00807-6. Epub 2022 Jan 10.
  PUBMED    35013556
REFERENCE   2  (bases 1 to 10135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-021-00807-6"; journalName: "Nat Cell Biol"; date: 
            "2022-01"; volume: "24"; issue: "1"; pages: "62-73"
COMMENT     Sequence Label: PB-PARP1-del.p119K120S–eGFP
FEATURES             Location/Qualifiers
     source          1..10135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   62..124
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     ncRNA           complement(486..500)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     524..540
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        567..870
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        871..1074
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1025..1045
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1103..1112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1112..1135
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      4220..4229
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4226..4228,4229..4231,4232..4945)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4226..4228,4229..4231,4232..4942)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4271..4292)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4532..4551)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4879..4900
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(4972..5545,5546..5548,5549..5557)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5138..5155)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5365..5384
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5639..6442
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5702..5721)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6312..6331
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6565..6686
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6602..6621)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6656..6675
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7125..7159
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(7305..7323)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7344..7360)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7344..7360)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7357..7379)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7368..7384
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7392..7398,7399..7416,7417..7422))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7437..7458
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7575..7592)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7746..8334)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7826..7845)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8505..9296,9297..9365))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9128..9147
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9366..9470)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9496..9951)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9633..9654)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9845..9864
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10093..10109
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10116..10135
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10116..10134
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgaattggcg gtattcacga cagcaggctg aataataaaa aaattagaaa ctattattta
       61 accctagaaa gataatcata ttgtgacgta cgttaaagat aatcatgcgt aaaattgacg
      121 catgtgtttt atcggtctgt atatcgaggt ttatttatta atttgaatag atattaagtt
      181 ttattatatt tacacttaca tactaataat aaattcaaca aacaatttat ttatgtttat
      241 ttatttatta aaaaaaaaca aaaactcaaa atttcttcta taaagtaaca aaacttttaa
      301 acattctctc ttttacaaaa ataaacttat tttgtacttt aaaaacagtc atgttgtatt
      361 ataaaataag taattagctt aacctataca taatagaaac aaattatact tattagtcag
      421 tcagaaacaa ctttggccta aagtagcccc ttgaattcaa caaaagaaaa aaacgataaa
      481 agatacatct gtggcttcac tagctagccc cagtcacgac gttgtaaaac gacggccagt
      541 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
      601 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      661 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      721 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      781 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      841 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      901 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      961 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     1021 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta
     1081 gtgaaccgtc agatccgcta gcgccaccat ggactacaaa gacgatgacg acaagcttgc
     1141 cgccgcgaat tcaatggcgg agtcttcgga taagctctat cgagtcgagt acgccaagag
     1201 cgggcgcgcc tcttgcaaga aatgcagcga gagcatcccc aaggactcgc tccggatggc
     1261 catcatggtg cagtcgccca tgtttgatgg aaaagtccca cactggtacc acttctcctg
     1321 cttctggaag gtgggccact ccatccggca ccctgacgtt gaggtggatg ggttctctga
     1381 gcttcggtgg gatgaccagc agaaagtcaa gaagacagcg gaagctggag gagtgacagg
     1441 caaaggccag gatggaattg gtagcaaggc agagaagact ctgggtgact ttgcagcaga
     1501 gtatgccaac agaagtacgt gcaaggggtg tatggagaag atagaaaagg gccaggtgcg
     1561 cctgtccaag aagatggtgg acccggagaa gccacagcta ggcatgattg accgctggta
     1621 ccatccaggc tgctttgtca agaacaggga ggagctgggt ttccggcccg agtacagtgc
     1681 gagtcagctc aagggcttca gcctccttgc tacagaggat aaagaagccc tgaagaagca
     1741 gctcccagga gtcaagagtg aaggaaagag aaaaggcgat gaggtggatg gagtggatga
     1801 agtggcgaag aagaaatcta aaaaagaaaa agacaaggat agtaagcttg aaaaagccct
     1861 aaaggctcag aacgacctga tctggaacat caaggacgag ctaaagaaag tgtgttcaac
     1921 taatgacctg aaggagctac tcatcttcaa caagcagcaa gtgccttctg gggagtcggc
     1981 gatcttggac cgagtagctg atggcatggt gttcggtgcc ctccttccct gcgaggaatg
     2041 ctcgggtcag ctggtcttca agagcgatgc ctattactgc actggggacg tcactgcctg
     2101 gaccaagtgt atggtcaaga cacagacacc caaccggaag gagtgggtaa ccccaaagga
     2161 attccgagaa atctcttacc tcaagaaatt gaaggttaaa aagcaggacc gtatattccc
     2221 cccagaaacc agcgcctccg tggcggccac gcctccgccc tccacagcct cggctcctgc
     2281 tgctgtgaac tcctctgctt cagcagataa gccattatcc aacatgaaga tcctgactct
     2341 cgggaagctg tcccggaaca aggatgaagt gaaggccatg attgagaaac tcggggggaa
     2401 gttgacgggg acggccaaca aggcttccct gtgcatcagc accaaaaagg aggtggaaaa
     2461 gatgaataag aagatggagg aagtaaagga agccaacatc cgagttgtgt ctgaggactt
     2521 cctccaggac gtctccgcct ccaccaagag ccttcaggag ttgttcttag cgcacatctt
     2581 gtccccttgg ggggcagagg tgaaggcaga gcctgttgaa gttgtggccc caagagggaa
     2641 gtcaggggct gcgctctcca aaaaaagcaa gggccaggtc aaggaggaag gtatcaacaa
     2701 atctgaaaag agaatgaaat taactcttaa aggaggagca gctgtggatc ctgattctgg
     2761 actggaacac tctgcgcatg tcctggagaa aggtgggaag gtcttcagtg ccacccttgg
     2821 cctggtggac atcgttaaag gaaccaactc ctactacaag ctgcagcttc tggaggacga
     2881 caaggaaaac aggtattgga tattcaggtc ctggggccgt gtgggtacgg tgatcggtag
     2941 caacaaactg gaacagatgc cgtccaagga ggatgccatt gagcacttca tgaaattata
     3001 tgaagaaaaa accgggaacg cttggcactc caaaaatttc acgaagtatc ccaaaaagtt
     3061 ctaccccctg gagattgact atggccagga tgaagaggca gtgaagaagc tgacagtaaa
     3121 tcctggcacc aagtccaagc tccccaagcc agttcaggac ctcatcaaga tgatctttga
     3181 tgtggaaagt atgaagaaag ccatggtgga gtatgagatc gaccttcaga agatgccctt
     3241 ggggaagctg agcaaaaggc agatccaggc cgcatactcc atcctcagtg aggtccagca
     3301 ggcggtgtct cagggcagca gcgactctca gatcctggat ctctcaaatc gcttttacac
     3361 cctgatcccc cacgactttg ggatgaagaa gcctccgctc ctgaacaatg cagacagtgt
     3421 gcaggccaag gtggaaatgc ttgacaacct gctggacatc gaggtggcct acagtctgct
     3481 caggggaggg tctgatgata gcagcaagga tcccatcgat gtcaactatg agaagctcaa
     3541 aactgacatt aaggtggttg acagagattc tgaagaagcc gagatcatca ggaagtatgt
     3601 taagaacact catgcaacca cacacaatgc gtatgacttg gaagtcatcg atatctttaa
     3661 gatagagcgt gaaggcgaat gccagcgtta caagcccttt aagcagcttc ataaccgaag
     3721 attgctgtgg cacgggtcca ggaccaccaa ctttgctggg atcctgtccc agggtcttcg
     3781 gatagccccg cctgaagcgc ccgtgacagg ctacatgttt ggtaaaggga tctatttcgc
     3841 tgacatggtc tccaagagtg ccaactactg ccatacgtct cagggagacc caataggctt
     3901 aatcctgttg ggagaagttg cccttggaaa catgtatgaa ctgaagcacg cttcacatat
     3961 cagcaagtta cccaagggca agcacagtgt caaaggtttg ggcaaaacta cccctgatcc
     4021 ttcagctaac attagtctgg atggtgtaga cgttcctctt gggaccggga tttcatctgg
     4081 tgtgaatgac acctctctac tatataacga gtacattgtc tatgatattg ctcaggtaaa
     4141 tctgaagtat ctgctgaaac tgaaattcaa ttttaagacc tccctgtggt cggtaccgcg
     4201 ggcccgggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     4261 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     4321 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     4381 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     4441 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     4501 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     4561 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     4621 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     4681 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     4741 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     4801 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     4861 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     4921 cggcatggac gagctgtaca agtaaccgcg gtggtaagaa ttccgccccc ccccctctcc
     4981 ctcccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     5041 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     5101 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcagggt
     5161 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     5221 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     5281 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     5341 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     5401 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     5461 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     5521 ttcctttgaa aaacacgatg ataatatggc cacaacctgt tacattgcac aagataaaaa
     5581 tatatcatca cgaacagtaa aactgtctgc ttacataaac agtaatacaa ggggtgttat
     5641 gggatcggcc attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     5701 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     5761 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     5821 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     5881 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     5941 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     6001 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     6061 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     6121 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     6181 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     6241 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     6301 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     6361 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     6421 ccttcttgac gagttcttct gaagcggccg cgactctaga tcataatcag ccataccaca
     6481 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     6541 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     6601 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6661 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agggtaccag gatccctaaa
     6721 gcgcatgctc cagactgttt attttttttt tcattttttt tttggtcgaa cgaggaggtt
     6781 caagtgcaga agagcagaga ggatatgctc atcgtctaaa gaactaccca ttttattata
     6841 tattagtcac gatatctata acaagaaaat atatatataa taagttatca cgtaagtaga
     6901 acatgaaata acaatataat tatcgtatga gttaaatctt aaaagtcacg taaaagataa
     6961 tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat
     7021 tgacaagcac gcctcacggg agctccaagc ggcgactgag atgtcctaaa tgcacagcga
     7081 cggattcgcg ctatttagaa agagagagca atatttcaag aatgcatgcg tcaattttac
     7141 gcagactatc tttctagggt taaaaaagat ttgcgcttta ctcgacctaa actttaaaca
     7201 cgtcatagaa tcttcgtttg acaaaaacca cattgtggcc aagctgtgtg acgcgacgcg
     7261 cgctaaagaa tggcaaacca agtcgcgcga gccagctttt gttcccttta gtgagggtta
     7321 atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7381 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7441 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7501 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7561 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7621 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7681 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7741 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7801 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7861 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7921 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7981 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8041 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8101 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8161 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     8221 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8281 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8341 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8401 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8461 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8521 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8581 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8641 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8701 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8761 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8821 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8881 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8941 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9001 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9061 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9121 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9181 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9241 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9301 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9361 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     9421 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9481 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     9541 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     9601 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     9661 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     9721 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     9781 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     9841 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     9901 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc
     9961 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
    10021 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
    10081 agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta taggg
//
