LOCUS       sequence-419962-        4756 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Level 0 Part: inducible Promoter (PsppQ) with two 
            regulatory genes sppK and sppR for the construction of Level 1 
            plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Li X, Schonberg PY, Wucherpfennig T, Hinze C, Sulaj F, Henle T, 
            Mascher T
  TITLE     Development of a Golden Gate Assembly-Based Genetic Toolbox for 
            Lactiplantibacillus plantarum and Its Application for Engineering 
            Monoterpenoid Biosynthesis.
  JOURNAL   ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 
            10.1021/acssynbio.4c00075. Epub 2024 Sep 10.
  PUBMED    39254046
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00075"; journalName: "ACS Synth Biol"; date: 
            "2024-09-20- 20"; volume: "13"; issue: "9"; pages: "2764-2779"
COMMENT     Sequence Label: pLPP5
FEATURES             Location/Qualifiers
     source          1..4756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2700..2757
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2781..2798)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2952..3540)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3032..3051)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3723..3817)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3838..4497)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4412..4431
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     4659..4677
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(4681..4724)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    4727..4748
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcggga ggtcgaccct tcaatagagt tcttaacgtt aatccgaaaa aaactaacgt
       61 taatattaaa aaataagatc cgcttgtgaa ttatgtataa tttgattaga ctaaagaata
      121 ggagaaagta tgatgatatt taaaaaactt tctcgttaag ataggttgtt ggtgagcatg
      181 ttatatacgg atgtatcggt ttccttaatg caaaattttg ttgctatctt attaattttt
      241 ctattatata gatatattca aagaaagata acatttaaac ggatcatatt agatatttta
      301 atagcgatta ttttttcaat attatatctg tttatttcag atgcgtcatt acttgtaatg
      361 gtattaatgc gattagggtg gcattttcat caacaaaaag aaaataagat aaaaacgact
      421 gatacagcta atttaattct aattatcgtg atccagttat tgttagttgc ggttgggact
      481 attattagtc agtttaccat atcgattatc aaaagtgatt tcagccaaaa tatattgaac
      541 aatagtgcaa cagatataac tttattaggt attttctttg ctgttttatt tgacggcttg
      601 ttctttatat tattgaagaa taagcggact gaattacaac atttaaatca agaaatcatt
      661 gaattttcgt tagaaaaaca atattttata tttatattta ttttatttat agtaatagaa
      721 attattttag cagttgggaa tcttcaagga gtaacagcca cgatattatt aaccattatc
      781 attatttttt gtgtccttat cgggatgact ttttggcaag tgatgctttt tttgaaggct
      841 tattcgattc gccaagaagc caatgaccaa ttggtccgga atcaacaact tcaagattat
      901 ctagtcaata tcgaacagca gtacaccgaa ttacggcgat ttaagcatga ttatcaaaac
      961 atcttattat cgttggagag ttttgccgaa aagggcgatc agcaacagtt taaggcgtat
     1021 taccaagaat tattagcaca acggccaatt caaagtgaaa tccaaggggc agtcattgca
     1081 caactcgact acttgaaaaa tgatcctatt cgaggattag tcattcaaaa gtttttggca
     1141 gccaaacagg ctggtgttac tttaaaattc gaaatgaccg aaccaatcga attagcaacc
     1201 gctaatctat taacggttat tcggattatc ggtattttat tagacaatgc gattgaacaa
     1261 gccgttcaag aaaccgatca attggtgagt tgtgctttct tacaatctga tggtttaatc
     1321 gaaattacga ttgaaaatac ggccagtcaa gttaagaatc tccaagcatt ttcagagtta
     1381 ggctattcaa cgaaaggcgc tggtcggggg actggtttag ctaatgtgca ggatttgatt
     1441 gccaaacaaa ccaatttatt cttagaaaca cagattgaaa atagaaagtt acgacagaca
     1501 ttgatgatta cggaggaaac ttaatttgta tcccgtttat ttattagagg atgatttaca
     1561 gcaacaagcg atttatcagc aaattatcgc gaatacgatt atgattaacg aatttgcaat
     1621 gactttaaca tgcgctgcca gtgatactga gacattgttg gcggcaatta aggatcagca
     1681 acgaggttta ttctttttgg atatggaaat tgaggataac cgccaagccg gtttagaagt
     1741 ggcaactaag attcggcaga tgatgccgtt tgcgcaaatt gtcttcatta caacccacga
     1801 ggaactgaca ttattaacgt tagaacgaaa aatagcgcct ttagattaca ttctcaagga
     1861 ccaaacaatg gctgaaatca aaaggcaatt gattgatgat ctattgttag ctgagaagca
     1921 aaacgaggcg gcagcgtatc accgagaaaa tttatttagt tataaaatag gtcctcgctt
     1981 tttctcatta ccattaaagg aagttgttta tttatatact gaaaaagaaa atccgggtca
     2041 tattaatttg ttagccgtta ccagaaaggt tacttttcca ggaaatttaa atgcgctgga
     2101 agcccaatat ccaatgctct ttcggtgtga taaaagttac ttagttaacc tatctaatat
     2161 tgccaattat gacagtaaaa cacggagttt aaaatttgta gatggcagtg aggcaaaagt
     2221 ctcgttccgg aaatcacggg aactagtggc caaattaaaa caaatgatgt agcgcctgca
     2281 gcacgccaaa tgatcccagt aaaaagccac ccgcatggcg ggtggctttt tattagccct
     2341 agaagggctt cccacacgca tttcagcgcc ttagtgcctt agtttgtgaa tcataggtgg
     2401 tatagtcccg aaatacccgt ctaaggaatt gtcagatagg cctaatgact ggcttttata
     2461 atatgagata atgccgactg tactttttac agtcggtttt ctaatgtcac taacctgccc
     2521 cgttagttga agaaggtttt tatattacag ctccagatct accggtttaa tttgaaaatt
     2581 gatattagcg tttaacagtt aaattaatac gttaataatt tttttgtctt taaataggga
     2641 tttgaagcat aatggtgtta cttgagacca ctcatcgcga ctagtagcgg ccgctgcagt
     2701 ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta
     2761 cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2821 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2881 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2941 ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3001 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3061 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3121 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3181 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3241 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3301 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3361 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     3421 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3481 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3541 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3601 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3661 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt
     3721 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
     3781 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
     3841 cgccccgccc tgccactcgt cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     3901 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     3961 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     4021 gtttaaatca aaactggtga aactcaccca gggattggct gatacgaaaa acatattctc
     4081 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     4141 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagtgatg aaaacgtttc
     4201 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     4261 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
     4321 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     4381 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     4441 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
     4501 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
     4561 ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg
     4621 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga
     4681 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
     4741 ttctagagcg atgagt
//
