LOCUS       sequence-419964-        2998 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Level 0 Part: resistance cassette (ermBL) for the 
            construction of Level 1 plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Li X, Schonberg PY, Wucherpfennig T, Hinze C, Sulaj F, Henle T, 
            Mascher T
  TITLE     Development of a Golden Gate Assembly-Based Genetic Toolbox for 
            Lactiplantibacillus plantarum and Its Application for Engineering 
            Monoterpenoid Biosynthesis.
  JOURNAL   ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 
            10.1021/acssynbio.4c00075. Epub 2024 Sep 10.
  PUBMED    39254046
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00075"; journalName: "ACS Synth Biol"; date: 
            "2024-09-20- 20"; volume: "13"; issue: "9"; pages: "2764-2779"
COMMENT     Sequence Label: pLPR1
FEATURES             Location/Qualifiers
     source          1..2998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(96..684)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(176..195)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     RBS             2127..2138
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      2852..2923
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2939..2966
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
       61 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      121 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      181 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      241 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      301 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      361 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      421 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      481 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
      541 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      601 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      661 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      721 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      781 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
      841 acttggtctg acagctcgag gcttggggtc tcgtgctaga gtgtgttgat agtgcattat
      901 cttaaaattt tgtataatag gaattgaagt taaattagat gctaaaaata ggaattgaag
      961 ttaaattaga tgctaaaaat ttgtaattaa gaaggaggga ttcgtcatgt tggtattcca
     1021 aatgcgtaat gtagataaaa catctactgt tttgaaacag actaaaaaca gtgattacgc
     1081 agataaataa atacgttaga ttaattccta ccagtgacta atcttatgac tttttaaaca
     1141 gataactaaa attacaaaca aatcgtttaa cttcaggaga gattacatga acaaaaatat
     1201 aaaatattct caaaactttt taacgagtga aaaagtactc aaccaaataa taaaacaatt
     1261 gaatttaaaa gaaaccgata ccgtttacga aattggaaca ggtaaagggc atttaacgac
     1321 gaaactggct aaaataagta aacaggtaac gtctattgaa ttagacagtc atctattcaa
     1381 cttatcgtca gaaaaattaa aactgaatac tcgtgtcact ttaattcacc aagatattct
     1441 acagtttcaa ttccctaaca aacagaggta taaaattgtt gggaatattc cttacaattt
     1501 aagcacacaa attattaaaa aagtggtttt tgaaagccgt gcgtctgaca tctatctgac
     1561 tgttgaagaa ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt
     1621 gcacactcaa gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa
     1681 accaaaagta aacagtgtct taataaaact tacccgccat accacagatg ttccagataa
     1741 atattggaag ctatataagt actttgtttc aaaatgggtc aatcgagaat atcgtcaact
     1801 gtttactaaa aatcagtttc gtcaagcaat gaaacacgcc aaagtaaaca atttaagtac
     1861 cattacttat gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa
     1921 ataattctat gagtcgcttt tttaaatttg gaaagttaca cgttaggtct gagacccgga
     1981 aagtgaaacg tgatttcatg cgtcattttg aacattttgt aaatcttatt taataatgtg
     2041 tgcggcaatt cacatttaat ttatgaatgt tttcttaaca tcgcggcaac tcaagaaacg
     2101 gcaggttcgg atcttagcta ctagagaaag aggagaaata ctagatggtt tctaaaggtg
     2161 aagcagtgat caaagaattt atgcgcttca aagttcacat ggaaggttct atgaacggcc
     2221 acgaatttga aattgaaggt gaaggcgaag gtcgtccata cgaaggtact caaaccgcaa
     2281 aactgaaagt taccaaaggt ggtccactgc cattttcttg ggatattctg tctccacaat
     2341 ttatgtacgg ttctcgtgca tttatcaaac acccagcaga tattccagac tactacaaac
     2401 aatcttttcc ggaaggtttc aaatgggaac gtgttatgaa ttttgaagat ggtggtgcag
     2461 ttacggttac ccaagatacc tctctggaag atggtactct gatctacaaa gttaaactgc
     2521 gtggtactaa ctttccacca gatggtccag ttatgcagaa aaaaaccatg ggttgggaag
     2581 catctaccga acgtctgtac ccagaagatg gcgttctgaa aggtgatatc aaaatggcac
     2641 tgcgtctgaa agatggcggt cgttacctgg cagatttcaa aaccacctac aaagcgaaaa
     2701 aaccagttca aatgccaggt gcatacaacg ttgatcgtaa actggatatt accagccaca
     2761 acgaagatta caccgttgtt gaacaatacg aacgttctga aggccgtcac tctaccggtg
     2821 gtatggatga actgtacaaa taaccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     2881 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     2941 ctcaccttcg ggtgggcctt tctgcgttta tacagctcac tcaaaggcgg taatacgg
//
