LOCUS       sequence-419965-       27026 bp    DNA     circular UNA 15-DEC-2025
DEFINITION  A plasmid containing a 17,271 bp fragment of the P. tricornutum 
            chloroplast genome. The yeast marker URA3 was integrated into the 
            chloroplast fragment, which is flanked by SapI sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 27026)
  AUTHORS   Walker EJL, Pampuch M, Chang N, Cochrane RR, Karas BJ
  TITLE     Design and assembly of the 117-kb Phaeodactylum tricornutum 
            chloroplast genome.
  JOURNAL   Plant Physiol. 2023 Dec 19:kiad670. doi: 10.1093/plphys/kiad670.
  PUBMED    38114089
REFERENCE   2  (bases 1 to 27026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 Dec 19:kiad670. doi: 10.1093/plphys/kiad670."
COMMENT     Sequence Label: pCHAP3_URA
FEATURES             Location/Qualifiers
     source          1..27026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1238..1912)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(1913..2060)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     misc_feature    2114..2617
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     misc_feature    2779..3029
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(3032..3787)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      3878..4097
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      4165..4696
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      4173..4787
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     CDS             complement(15550..16353)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCCQQN"
     promoter        complement(16354..16569)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     promoter        22933..23035
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             23036..23695
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(23102..23121)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    23915..23936
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(23951..23956,23957..23974,23975..23981)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    23989..24005
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     23994..24016
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     24013..24029
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24013..24029
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             24025..24300
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGETIEYSSLHACRSTLEDPRVPSSNSPYSESYYNSL
                     AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS"
     misc_feature    24072..24128
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(24130..24149)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(24131..24149)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(24156..24173)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(24156..24172)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(24165..24187)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(24380..24399)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    24467..24500
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    24518..24916
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     misc_feature    25176..25649
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(25722..26693)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(join(26693..27026,1..842))
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
ORIGIN
        1 aaaaactgca gtgcggaggt gtagtcaaac aactcagcag gcgtgggaac aatcagcaca
       61 tcagcagcac atacgacatt aatcgtgccg atacccaggt taggcgcgct gtcaataact
      121 atgacatcat agtcatgagc aacagtttca atggccagtc ggagcatcag gtgtggatcg
      181 gtgggcagtt taccttcatc aaatttgccc attaactcag tttcaatacg gtgcagagcc
      241 agacaggaag gaataatgtc aagccccggc cagcaagtgg gctttattgc ataagtgaca
      301 tcgtcctttt ccccaagata gaaaggcagg agagtgtctt ctgcatgaat atgaagatct
      361 ggtacccatc cgtgatacat tgaggctgtt ccctgggggt cgttaccttc cacgagcaaa
      421 acacgtagcc ccttcagagc cagatcctga gcaagatgaa cagaaactga ggttttgtaa
      481 acgccacctt tatgggcagc aaccccgatc accggtggaa atacgtcttc agcacgtcgc
      541 aatcgcgtac caaacacatc acgcatatga ttaatttgtt caattgtata accaacacgt
      601 tgctcaaccc gtcctcgaat ttccatatcc gggtgcggta gtcgccctgc tttctcggca
      661 tctctgatag cctgagaaga aaccccaact aaatccgctg cttcacctat tctccagcgc
      721 cgggttattt tcctcgcttc cgggctgtca tcattaaact gtgcaatggc gatagccttc
      781 gtcatttcat gaccagcgtt tatgcactgg ttaagtgttt ccatgagttt cattctgaac
      841 atcctttaat cattgctttg cgttttttta ttaaatcttg caatttactg caaagcaaca
      901 acaaaatcgc aaagtcatca aaaaaccgca aagttgttta aaataagagc aacactacaa
      961 aaggagataa gaagcgcaca tacctcagtc acttattatc actagcgtca agtccagact
     1021 cctgtgtaaa atgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     1081 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttcctga
     1141 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatagat cggcaagtgc
     1201 acaaacaata cttaaataaa tactactcag taataaccta tttcttagca tttttgacga
     1261 aatttgctat tttgttagag tcttttacac catttgtctc cacacctccg cttacatcaa
     1321 caccaataac gccatttaat ctaagcgcat caccaacatt ttctggcgtc agtccaccag
     1381 ctaacataaa atgtaagctt tcggggctct cttgccttcc aacccagtca gaaatcgagt
     1441 tccaatccaa aagttcacct gtcccacctg cttctgaatc aaacaaggga ataaacgaat
     1501 gaggtttctg tgaagctgca ctgagtagta tgttgcagtc ttttggaaat acgagtcttt
     1561 taataactgg caaaccgagg aactcttggt attcttgcca cgactcatct ccatgcagtt
     1621 ggacgatatc aatgccgtaa tcattgacca gagccaaaac atcctcctta ggttgattac
     1681 gaaacacgcc aaccaagtat ttcggagtgc ctgaactatt tttatatgct tttacaagac
     1741 ttgaaatttt ccttgcaata accgggtcaa ttgttctctt tctattgggc acacatataa
     1801 tacccagcaa gtcagcatcg gaatctagag cacattctgc ggcctctgtg ctctgcaagc
     1861 cgcaaacttt caccaatgga ccagaactac ctgtgaaatt aataacagac atactccaag
     1921 ctgcctttgt gtgcttaatc acgtatactc acgtgctcaa tagtcaccaa tgccctccct
     1981 cttggccctc tccttttctt ttttcgaccg aattaattct taatcggcaa aaaaagaaaa
     2041 gctccggatc aagattgtac gtaaggtgac aagctatttt tcaataaaga atatcttcca
     2101 ctactgccat ctggatcgct tgcctgtaac ttacacgcgc ctcgtatctt ttaatgatgg
     2161 aataatttgg gaatttactc tgtgtttatt tatttttatg ttttgtattt ggattttaga
     2221 aagtaaataa agaaggtaga agagttacgg aatgaagaaa aaaaantaaa caaaggttta
     2281 aaaaatttca acaaaaagcg tactttacat atatatttat tagacaagaa aagcagatta
     2341 aatagatata cattcgatta acgataagta aaatgtaaaa tcacaggatt ttcgtgtgtg
     2401 gtcttctaca cagacaagat gaaacaattc ggcattaata cctgagagca ggtagagcaa
     2461 ggtaaaaggt agtatttgtt ggcgatcccc ctagagtctt ttacatcttc ggaaaacaaa
     2521 aactattttt tctttaattt ctttttttac tttctatttt taatttatat atttatatta
     2581 aaaaatttaa attataatta tttttatagc acgtgatgct cgccgcagcc gtgtaaccga
     2641 gcatagcgag cgaactggcg aggaagcaaa gaagaactgt tctgtcagat agctcttacg
     2701 ctcagcgcaa gaagaaatat ccaccgtggg aaaaactcca ggtagaggta cacacgcgga
     2761 tagccaattc agagtaataa actgtgataa tcaaccctca tcaatgatga cgaactaacc
     2821 cccgatatca ggtcacatga cgaagggaaa gagaaggaaa tcaactgtga caaactgccc
     2881 tcaaatttgg cttccttaaa aattacagtt caaaaagtat gagaaaatcc atgcaggctg
     2941 aaggaaacag caaaactgtg acaaattacc ctcagtaggt cagaacaaat gtgacgaacc
     3001 accctcaaat ctgtgacaga taaccctcag actatcctgt cgtcatggaa gtgatatcgc
     3061 ggaaggaaaa tacgatatga gtcgtctggc ggcctttctt tttctcaatg tatgagaggc
     3121 gcattggagt tctgctgttg atctcattaa cacagacctg caggaagcgg cggcggaagt
     3181 caggcatacg ctggtaactt tgaggcagct ggtaacgctc tatgatccag tcgattttca
     3241 gagagacgat gcctgagcca tccggcttac gatactgaca cagggattcg tataaacgca
     3301 tggcatacgg attggtgatt tcttttgttt cactaagccg aaactgcgta aaccggttct
     3361 gtaacccgat aaagaaggga atgagatatg ggttgatatg tacactgtaa agccctctgg
     3421 atggactgtg cgcacgtttg ataaaccaag gaaaagattc atagcctttt tcatcgccgg
     3481 catcctcttc agggcgataa aaaaccactt ccttccccgc gaaactcttc aatgcctgcc
     3541 gtatatcctt actggcttcc gcagaggtca atccgaatat ttcagcatat ttagcaacat
     3601 ggatctcgca gataccgtca tgttcctgta gggtgccatc agattttctg atctggtcaa
     3661 cgaacagata cagcatacgt ttttgatccc gggagagact atatgccgcc tcagtgaggt
     3721 cgtttgactg gacgattcgc gggctatttt tacgtttctt gtgattgata accgctgttt
     3781 ccgccatgac agatccatgt gaagtgtgac aagtttttag attgtcacac taaataaaaa
     3841 agagtcaata agcagggata actttgtgaa aaaacagctt cttctgaggg caatttgtca
     3901 cagggttaag ggcaatttgt cacagacagg actgtcattt gagggtgatt tgtcacactg
     3961 aaagggcaat ttgtcacaac accttctcta gaaccagcat ggataaaggc ctacaaggcg
     4021 ctctaaaaaa gaagatctaa aaactataaa aaaaataatt ataaaaatat ccccgtggat
     4081 aagtggataa ccccaaggga agttttttca ggcatcgtgt gtaagcagaa tatataagtg
     4141 ctgttccctg gtgcttcctc gctcactcga ccgggagggt tcgagaaggg ggggcacccc
     4201 ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca aggtttataa
     4261 atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac gggcggaaac
     4321 ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc
     4381 ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag
     4441 tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc cacatcatct
     4501 gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc cagctccacg
     4561 tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc
     4621 ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc gcgaggtatc
     4681 cacaacgccg gcggccggcc gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg
     4741 tttgcagggc catagacggc cgccagccca gcggcgaggg caaccaggct cttcatgcac
     4801 ctgcgcgaca tgaataaggt aattcaatac cttgctcctc agcagcatct aaaacaaata
     4861 catcatcatt acaatcaatt gttgtattga tatcatgttc ttcacttaat aatgtcactt
     4921 tgtaagtaac catataactc cttatgttta ataattataa agtaaaatat tttcttccac
     4981 cttactacat tacattatac tacgtaaatt tgatacaaag aaattttttt tattaaaatt
     5041 ttaaaagatt ttaaattcta tttaaaagtt tgttttaaac gactagcttt tccacgtaaa
     5101 tttcgtagat agtatagttt cgctcgacga acttttgatg aacgtaaaac tgtaatagaa
     5161 tcaatttttg gcgaatgaac taaaaaaaca cgctcaattc cgataccctg taatattttt
     5221 cgaacagtaa ttgtcatatt aatagaacta tttttctttg caataatagt tccctcgtaa
     5281 aactgaactc tttctttagt gccttcgata attttaacac caattctaac attatcacca
     5341 attttaagtg tcggaagatc ttttttaata aatttacttt ctatagcatc tattaccttt
     5401 tgagtcttta actttaacat gaattctaac gattattgta attattttta taagaaaaag
     5461 cttacaatta gatctagaaa aaaacaacat taaaatatat tttctgcatt cgactgggtt
     5521 cgaaccagtg acgttccaga ggaaaacgga ttatgagtcc ggtgccttca accactcggc
     5581 cacgaatgca aaaatggaac tgatgggaat tgaacccatg tccatttaaa aatatctaat
     5641 gtactcgttc acaggtatag tttcaaagaa caaatgttta ataaaattag tctaaactac
     5701 aaaggtttaa agccattaga tgtagtgaac aaaattaggt aataaaattt agttactcag
     5761 ttaaacagca aattgtaata aatttgaata atttttttta atattaaaaa ttactgcttt
     5821 agaaacgaaa gaaattatat taatagcatt tatttaaaat tttgtagatt tttacgaagg
     5881 atacaagctt cgccctgaat catacattag ttatttttaa atgtcgaaac cagatcagcc
     5941 ccacatattt ttaaaatatt ataaaaatgg aaccataagc atgaagggaa tcgaaccctt
     6001 gacttccaga atcggaatct gatgctctat ccactgagct acatgctttc cagaactatt
     6061 acattttcac tttaaagcat actaatctat tattaataaa attgtttaaa aagtggaaat
     6121 ttttccactt tttaaacaat tttattaata atagatttta ccgccgcccc atttttctgt
     6181 tacaattttc ggttgtaata atatatgacc agccatggca gttaaatctt catctgttac
     6241 tgaacgcata cgaggataaa tgtccgcact tttgatactt ggatgaactt cagcaattga
     6301 ttctaaacca tcatatgttg ttggattttt taaaaaatca accaaacctg ctatgttatc
     6361 gcgacgaggt gtcgctaaac ttaatgcttc tggatctaaa cctacgtttg gatttgtttt
     6421 tgtaactcca ccaacatggc aagcaccaca agttgcatta aataatcttt tcccacgttt
     6481 aacttgttca ggagttaaaa cgattgtttt acctgaagaa tcgacaacga ctgttcgggt
     6541 tgcttcgtct aaatcaatag ctgatgccgt tcctataaaa atactaaaaa tagtgaaaaa
     6601 tactaaagaa aagagttgag aagacttctt aaacataatt taaaattatt atttacaaat
     6661 ttgaaaccaa gataataaaa cgagaaagtt tacttccttt tttcctttgt taattttgcc
     6721 atgttagcaa ttaaaccccg tagtcgaata ttttcccatc ccgctactaa tactgtcaca
     6781 ataattagaa cttgactaaa aagaagaatt ggatctaatc tccaaccatg gatcattaga
     6841 atacaactgt ataatagacc aatagtggca aaaaagatat cttcatctct cgctacttcg
     6901 ggcttaacat ttctaagaaa ataaagtatt aacactccca aacttacgat aattcctaaa
     6961 aaaatatttg gaccgaaact aaaatttatc ataatatagc taactttaaa taaactttat
     7021 attatattag ctagaaatag tataaataaa tatgcgattt ataaattaaa tctgtaattt
     7081 taatatgtac tttataaaaa attaattttg ttgactctaa aatcaatata atatctaact
     7141 ctactatatt acaaaactaa cttctcgtaa cgcgatcact ttgactgata aaaactaaaa
     7201 ccccgccaat cgctaatgca ataaacgcac cagcaaataa actagcgata aaacttccta
     7261 atgaaggtgt cattttttat ttcctgttgt ttaaaataat aaggttaaaa atagtaagat
     7321 tgactacaat gaatattgta tcaacttttg taaaaaaaat ataattaata ttaatcgaat
     7381 catatttttc atattattga gatactgact aagaaattga cgaataacta aaactaaaaa
     7441 aaattggatc cggcatatag atatagattt aatactaacc ttgatatttt attttatgct
     7501 gctatcttac gatcctgaca tactaaacaa attcaaaatt atatcaaata ctatacccaa
     7561 agcttattat aggcaaattt tgaaaaaccg tcaactgcaa actaatttaa aactttattg
     7621 taattttctt tagatcgtgc tacaatatat ttggaagccc ggatagctca gttggtagag
     7681 cagtggattg aagatcctcg tgtcaccagt tcgaatctgg ttctgggcat tagctaacta
     7741 catttacgtc tgcgtcgttt gcattaagaa atttcgttct tttttcatca aattttaatt
     7801 ttactgttcc gaccggacca tttcgttgtt ttgcaataat gagttcagtc aattgttttg
     7861 tcttatctaa agatttgttt tcattatagt attcatttcg ataaagcatt aatactaaat
     7921 cagcatcttg ttcaatagag ccactttctc gtaaatcgga taaaattggc tttttatcta
     7981 gtctattttc aacatttcga cttaattggg ataaagcaat aatcggaaca ttaaattccc
     8041 gcgcaatcgt ttttaaggaa cgagttatat ttgagagttc ttggacacga ttttcactct
     8101 tattatctga attttgtaat aactgaagat aatctattat tatcaaacca attcggtttt
     8161 gttcaaataa aattgttttg attttcgatc gaatatcttg aattgataaa ttagatgtgt
     8221 catcaataaa aaacggtaat ttagaaacga tttttataat tttatttaat ttaatccaat
     8281 cattttgata aagcttgccg cttcggaggc gcatttgatt aatatttgtt tccattgcta
     8341 ataaacgata cataatttgt tctttcgaca tttctaagct cagaaataaa accggtagtc
     8401 tcgaattttt aattgtattg attgcaatat ttaatcctaa cgctgttttt cccatagatg
     8461 gtcgagctgc gataataatc aaatccgatt tctgaaaacc ttgtgttaaa gaatcaaggt
     8521 catagaaacc ggaaggtaat ccgaaaagtg aaggatttaa tgatttttct tttaactcga
     8581 aaaaaatatt attaagtaac tctgcactac taaataattt ttgcgaggta atttcatttg
     8641 ttaaattgaa tagcttattt tcaaaatcgc ctaatatttg ttcaagagga agattagtaa
     8701 tataacctga attaatgact tcataaccaa gtttaattaa agaacgtcta ataaacttat
     8761 cttttaccaa actaatatac tcttcaaggt aaaccaaatt agggatttga ctcattaatt
     8821 caattaaaac tttaactcca ccaatttttt gtaaaagccc attatcttgt aaaaaagtga
     8881 gtaaggttag aatatctata cactttttat tcttatataa aatcataacg gctttataaa
     8941 tttcttgatg atttttaaaa taaaaagctt catagggtag tttacgtaat gttatttcga
     9001 ttgcttctga attaatgagt agacaactaa gaatcatttt ttccgctaag aaattatgag
     9061 gtaaatattt atcgataatt aattgatcaa cattgaatgg agtttgttgc atttaatgaa
     9121 ataaaaatat ggattaaaat ttaggcttgt aatttcaatt tattaagttt acaatgaact
     9181 cgggcgtcct tagttcagtt ggtagaacgc tagtctccaa aattagatgt cgagggttca
     9241 agtccttcag ggcgcgtttc tctttcttaa gaggttttta attttttaat atcatagaac
     9301 ttattttaga acaactataa cgttttgatt tcgagtaatc aagttataag tctattaaat
     9361 taaaacgatt aaaataatta ttagcaaact ataatattat atttgtatgg ccaaaaaaat
     9421 tactgcgttg attaagttag ctttacctgc tagtaaagcg actccggcac cgcctgtggg
     9481 accagcttta ggtcaacacg gtgtaaatat tgccgcattt tgtaaagagt ataatgctcg
     9541 tactaatgat aaagcaggat taattattcc tgtagaaatt tcagtttatg aagatagaag
     9601 ttacacattc gttttgaaaa cacctcctgc ttcagtacta ttagcaaatg ctgcaaaaat
     9661 taagaagggt tcgtcgaatc ctaatacggt aagtgttggg tcaataacaa aaactcagtt
     9721 agaagaaatt gcaaacatta aattactaga tttaaatact acgaaaatca gtagtgctat
     9781 gaaaattgtt gaaggaacgg ctcgtaatat gggtatctcc attttagatt aagtgaagat
     9841 taaataaatt tttaacggag aaaagttatg cagaaagtgt cgcgtcgtca taaagaaaat
     9901 ttcgaaagaa cgaagaatat tgtttattca aatatagacg aggctattgc cgttttaaaa
     9961 gaaacagcaa caacaaaatt tgttgaatca gtagaattac atgctaatct aaatattgat
    10021 ccaaaatatg ccgatcagca attgcgtacg actgttacac taccaaacgg catcggcaag
    10081 caagtagtta tagcagttct gacaaatgaa gaaaattttg aagaggctaa atcctcaggt
    10141 ggggatatcg ttggaaatga tgaattgatt gctcaaatta ctcaaggaca gattaatttt
    10201 gatttattaa ttgcaacacc caacatgatg cctaaattag ccaaattagg tcgaatgtta
    10261 ggacccaaag gcttaatgcc ctcaccaaaa tcaggtaccg ttactagtac tctgcaatcg
    10321 acattaacag aatttaaaaa aggtaagttt gaatataagg ctgacaaaac tggaatcgtt
    10381 catgttaatt ttggaaaatc aaattttaca aacgaacaac tagttgaaaa tttaaaagct
    10441 ctataccaat caattgagca aaatcgtccg tcgggtgtaa aaggtaagta ctttaaaagt
    10501 gtatcaattt gtactacaat gggaccatcc gtaaagttag acttagagat atttgcataa
    10561 attttagatt catttcatta ttttactcaa aactaaaatt ttataatatg atttaaattt
    10621 attatgtcag aaaaaattga tcaaatcgtt gaagatttaa aaacattaac attactagaa
    10681 gcttcagaat tagtaacaaa aattgaagaa acatttggtg tcgatgcttc agcagcagct
    10741 agtggtggtg ttgtaatggc agctgctcca gccgtagttg aagaagttga agaaaaaaca
    10801 gaatttaatc taatgcttga tgaagttcca gctgataaaa aaattgctgt tttgaaggtt
    10861 gtacgtagtt taacaggtct aggcttaaaa gaagcgaaag aattagtaga atcagcacca
    10921 aaacaaattc aagagggttt aggaaaagac gcagcagagg aagctaaaaa acaaattgaa
    10981 gcagcgggtg gtaaagcttc attgacttaa ttttaaattt agtttttgat gattaataaa
    11041 ttaaccatga ttgaaaattt tggttaattt attaacacgt tagttgacag cgactatgaa
    11101 gatatgttat aatttgttta tatgttgata ctctaaggaa ataatatata taccggttgt
    11161 taagtctgga tctaaatgtg attttgtaat tttttatctg tcgcggagta gagcagcctg
    11221 gtagctcgtt gggctcataa cccgaaggtc aatggttcaa atccattctc cgctagaaat
    11281 tttcataatt ttaataaaaa attttttttt ttattaaaat tatatagtga acgttaaaca
    11341 tttataaagt tttacagatg acattaaatt tacaaacgcc gtttcctact tttggtggaa
    11401 gtactggagg ctggttacga tcagctgaag ttgaagaaaa atacgcaatt acttggacaa
    11461 gccccaaaga gcaaatattt gagatgccaa ttggtggagc agctatcatg cgtagtggag
    11521 aaaatttatt atacttagcg cgcaaagagc aatgtttagc attgggaacc caattacgta
    11581 catttaaaat taatgattat aaaatttatc gaattttccc aagtggagaa gttcaatatt
    11641 tacatccaaa agatggagtt tttcctgaaa aagtaaatcc aggtcgaact gccgttaata
    11701 accgcgattt ttcgattgga aaaaatccaa atccagcttc tattaagttt agtggaataa
    11761 atacgtatga aagttagtta atatctaaga ttagttttgt tactaatctt ttggcgagat
    11821 ggcagagttg gtcgattgcg tctgatttga aatcagatga atctttacag gttccgtggg
    11881 ttcgaatccc actctcgcct ttatttttta aaattgtttt gcttaaccgt gtcaaaattt
    11941 tctgaaaatt tttatgaata aagtttacga ttggttcgaa gaacgtctag aagttcaagc
    12001 aattgctgat gatatttcta gtaaatatgt accacctcat gtaaatattt tttattgttt
    12061 tggaggtctt gttttaacgt gttttttaat tcaagttgca actggttttg caatgacatt
    12121 ctattatcga cctagtgttg ttgacgcatt tgcatcagta gaatatatta tgacgagtgt
    12181 caattttggt tggttaattc gctcaatcca tcgttggtca gcaagtatga tggtgttgat
    12241 gatggtgtta cacgttttta gagtttattt aactggtgga tttaaaaaac cacgtgaatt
    12301 gacttgggtc acaggtgtca ctttatccgt tgttaccgtt tcatttggtg taacaggtta
    12361 ttcattacca tgggatcagg taggattttg ggcgtgtaaa attgtaactg gtgtaccagc
    12421 ggcagttcca attgtaggtg aacctttagt tttaatctta cgtggtggag aaagtgtagg
    12481 tcaaagtaca ttaacacgtt tctatagtgc tcatactttt gttttaccat tggcggcagc
    12541 agtattaatg ctaactcatt tcttaatgat tcgcaaacaa ggtatctcag gcccattata
    12601 aaggtgtttt aaagaaattt tgaacattaa atttttaacc aattataaaa taattattaa
    12661 taaattctat tatgtcagtc attaaaaaac cagatttaac tgatccaaaa ttacgtgcta
    12721 aattagctaa aggtatgggc cataactatt atggagaacc agcatggcca aatgatcttt
    12781 tatatgtctt cccacttacg atgttaggaa cattaacatg cattgtaggt ttatcagttt
    12841 tagcaccgac tcaactaggt gaaccagctg atccatttaa tacaccatta gaaattttac
    12901 cagaatggta ttttttcccg acatttaatt tattacgcgt gttaccgaat aaattattag
    12961 gagttttagc aatggcagca gttcctttag gattaatcac ggttccattt attgaaaacg
    13021 taaataaatt tcaaaatccg tttcgtcgcc cgcttgcgag tctaactttt atttttgggt
    13081 tttttacagc agtttggtta ggtataggtg catgtgtacc aattgacaaa gctatttcct
    13141 taggtttttg gtaaacaaaa gattttgatc actatctatt aatttgtagt gatcaaaatc
    13201 ttttgtctaa attttaatga tttagtaaat tcatttggat agcaatccca aaataaataa
    13261 atataccaaa accagttaaa atatttaacg atcgttgggc tgaactaggt gagcccaata
    13321 gttcgctttt tgtagcaaaa cttgctaatc cgacattttc ttgtgggatt cgtaaaaaaa
    13381 taatgacaat taaaaataga ctgatagcaa atgcaaatag ttttaacata tttttggact
    13441 aatttaaaaa ttttacacta tgaatgacta actatttata aaaatctaaa gtagttttaa
    13501 tcctcaattt caaagatctc tctaaatacc ttacccactt tatcacctga atcaattgtt
    13561 tctaatggat cttttcgtag tctatgacgt aagcataaag taataatact agcaatatca
    13621 tttatagtaa ctttatcacg tttatgatat gctgcatgag cttttgcagc ccgatttgtt
    13681 acaatatcac cacgtagtcc atctacatct aagcgactac atacttctga aattttaacc
    13741 cgtaaatcgt aatcaatttc aactgttgga agtaattttt gagcggctac gattcggtcg
    13801 cgtaattctt gttgttgaat ttcgtaattt tcaatccata ccattggact ttggtcaaat
    13861 gatgttcttt cttctacaac tttaacacgt aacataggat cttttacggt tctaatctca
    13921 gcgtgcatac cgaaacgatc gagtaactga ggacgtaact cgccttcctc gggatttcct
    13981 gatccaacta aaacaaaacg tgctgggtga cgaattgaaa tgccttcgcg ttcaactgtg
    14041 ttccaaccag atgctgcaga atctaataaa atatcgacaa gatgatcgtc taataagttt
    14101 acttcgtcta cgtaaagtag tcctctattt gctttagcta atagacctgg ttcaaaagct
    14161 tttatacctt cagttaaagc tttttcaata tcaatagtcc cacatactct atcttcagtt
    14221 gctcccagag gcaaatcaac cattggaatc ttgataaatt ccgtttcaat agcttcatcg
    14281 ttctgaattg ctgatttaac ttcgtttccc attaaatcaa gatctgattt atgtgaattg
    14341 aatggatcat ctttaactac ttcaatttct ggaagtaaat cagcaattgc gcgaattgtt
    14401 gttgattttc ctgttcctcg atcccccata atcataactc caccaatttt tggatcaatt
    14461 acgtttaatt gtaaagccaa tttcatttct tcttggccaa caattgctgt aaaaggaaat
    14521 acgggagtag taaatttttt taaatctttg gttaccataa cttattatcg attattaatg
    14581 taaacggttt ttaaaataat aatttctttt taataattta aagaacaatt attcatataa
    14641 tgtcgaacct aatctaatcg ctaaaattcc ggctaataac gcaatacata gcactgtata
    14701 aatttgtgaa tcgtaaatca tcaaaaactc cttatttcca attaaatttg ttttgattct
    14761 aaagaattaa atatcctaaa tgataataaa atatttctac aaatacttta acttcgtata
    14821 tgtaattgta aattaaatta ataaaatcat ttaatttaat ttaagtaaat ttttttacca
    14881 acttgatttt gttacacctg gtaataagca ttgatgagcc atttcacgaa aaacgtgacg
    14941 tgataaacca aaatcacgaa aaactccacg tggacgtcct gttttccaac atcgatttcg
    15001 aattcttgtt tttgcactat tgcgtggtaa cttctgaatt tttgaatgaa gttccaattt
    15061 taagttaaaa ctttcttcag tttggtattt ttttaataaa gttgcacgtt tcaaagcata
    15121 tttcttggtt agtttaatac gctttttctc acgctctatc atacttttct ttgccataat
    15181 taatagtttt ttgtttgaat agagaatttt tggaaattta ataagggtca gtgttgagtt
    15241 aatcttattc tatattaaaa attttagcaa gttaaagtca aaaaattcat agttaattta
    15301 ttactagaat tacgttaatt tagaagttaa aaactctggc gcgcctcaac gacaggagca
    15361 cgatcatgcg cacccgtggc caggacccaa cgctgcgggg ggggggggtt ttctttccaa
    15421 tttttttttt tttcgtcatt ataaaaatca ttacgaccga gattcccggc cgggtaataa
    15481 ctgatataat taaattgaag ctctaatttg tgagtttagt atacatgcat ttacttataa
    15541 tacagttttt tagttttgct ggcagcatct tctcaaatat gcttcccagc ctgcttttct
    15601 gtaacgttca ccctctacct tagcatccct tccctttgca aatagtcctc ttccaacaat
    15661 aataatgtca gatcctgtag agaccacatc atccacggtt ctatactgtt gacccaatgc
    15721 gtctcccttg tcatctaaac ccacaccggg tgtcataatc aaccaatcgt aaccttcatc
    15781 tcttccaccc atgtctcttt gagcaataaa gccgataaca aaatctttgt cgctcttcgc
    15841 aatgtcaaca gtacccttag tatattctcc agtagctagg gagcccttgc atgacaattc
    15901 tgctaacatc aaaaggcctc taggttcctt tgttacttct tccgccgcct gcttcaaacc
    15961 gctaacaata cctgggccca ccacaccgtg tgcattcgta atgtctgccc attctgctat
    16021 tctgtataca cccgcagagt actgcaattt gactgtatta ccaatgtcag caaattttct
    16081 gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc tttagcggct taactgtgcc
    16141 ctccatggaa aaatcagtca agatatccac atgtgttttt agtaaacaaa ttttgggacc
    16201 taatgcttca actaactcca gtaattcctt ggtggtacga acatccaatg aagcacacaa
    16261 gtttgtttgc ttttcgtgca tgatattaaa tagcttggca gcaacaggac taggatgagt
    16321 agcagcacgt tccttatatg tagctttcga catgatttat cttcgtttcc tgcaggtttt
    16381 tgttctgtgc agttgggtta agaatactgg gcaatttcat gtttcttcaa cactacatat
    16441 gcgtatatat accaatctaa gtctgtgctc cttccttcgt tcttccttct gttcggagat
    16501 taccgaatca aaaaaatttc aaggaaaccg aaatcaaaaa aaagaataaa aaaaaaatga
    16561 tgaattgaaa cccccccccc cccgatgcgc cgcgtgcggc tgctggagat ggcggacgcg
    16621 atggatatgt tctgccaagg gcgcgccagt ttttctggac ttttcaagcc tattggtata
    16681 caaaatttgt ctcaaacaaa attaaattga ttttttatct taattttttt tcatcttggc
    16741 ttataatgtt ataatagcta acttttatgt agtttagttg taagaaagtc aaaaaccatt
    16801 attatattga ggaatctatt actatggcat taccatggta tcgtgttcac actgttgttt
    16861 taaatgatcc aggaagatta attgctgttc acattatgca tacagctttg gttgcaggtt
    16921 gggctggatc aatggcctta tacgaattag ctgtattcga tccatcagat ccagttttaa
    16981 atccgatgtg gcgtcaaggg atgtttgtta tgccatttat gacaagacta ggtattactg
    17041 attcttgggg tggttggagt attactggtg aaagtgtatc taaccctggt ctttggagtt
    17101 tcgaaggtgt agcattatcg catattgttt tgtcaggtat gtgcttttta gctgcaatct
    17161 ggcattgggt ttattgggat ttagaattat tccgtgatcc acgaacaggt gaacctgctt
    17221 tagatttacc aaaaatcttt ggtattcatt tatttttatc aggtttatta tgttttggtt
    17281 tcggtgcatt ccacgtaaca ggtttatttg gtcctggtat ctgggtttca gatgcgtatg
    17341 gaatcacagg taaagtacaa ccagtggcgc catcgtgggg tgcagacgga tttaacccgt
    17401 ttaacccagg tggtattgct gcgcaccata ttgcagcagg tctcttcggt attcttgcag
    17461 gcgttttcca cttaacagtt cgtccaccac aacgtttata tcgggcttta agaatgggta
    17521 atattgaaac agttttatct agtagtattt cggccgtttt ctttgcagcg tttgtaacat
    17581 cagggacaat gtggtatgga gctgctgcaa caccaattga attatttggc ccaacacgtt
    17641 atcagtggga tagcggttat ttccaacaag aaattgagcg tcaagttgaa gcttcagtaa
    17701 gtgaaggttt atcggaaagt caagcatggt caagaattcc agataaatta gccttttatg
    17761 attatattgg taataaccca gctaaaggcg gtttattccg tgcgggtccg atgaataaag
    17821 gagatggtat cgcagaagct tggctaggtc atccaatttt ccgtgacaaa gaaggtcgtg
    17881 agttaactgt acgtcgtatg ccagctttct ttgaaacatt ccctgtaatt ttagtagata
    17941 aagatggtat tattcgagct gatattccat tccgtcgtgc agaatcaaaa tatagtattg
    18001 aacaagtggg agtaacagtt gatttttacg gtggtaaatt aaatggtcaa acatttaaag
    18061 atgcaccaac cgttaaaaag tttgctcgta aatcacaatt aggtgaagtt tttgaattcg
    18121 atagaacaag tttagaatca gatggtgtgt tcagaagtag tccacgtggt tggtatacct
    18181 atggtcatgc taactttgct ttattattct tctttggaca tctatggcat ggtggtagaa
    18241 caattttccg tgacgtattt actggaattg gtgctgaagt tacggagcaa gtagaatttg
    18301 gtgtattcca aaaacttggt gataaaacaa cgaaaaaaca gggtgctgta taaaatacag
    18361 ttaatctttt aagttcttta tattaaaata ggatcaaaca atggaagcgt tagtttatac
    18421 atttttactt attggcacct taatcgtaat tttctttgcc gtatttttca gagaaacacc
    18481 tcgaattatt aaaaaataaa accaattaaa atggttttat ttttttaatc ctcatgttct
    18541 tcgaatggat cacgtaaatt ttttgaagcg ggtccaaaag ccgtataaac agaatatgtg
    18601 gtaattccta agagtagact tgagacaaaa attacgataa tagttgctgt ttccatgtta
    18661 tataatatta aaactaacag tttaacatta tataacatat agtagaaaaa ttaatttatt
    18721 aaaaaataaa tctttttatg gcattacgaa caagattagg tgaactttta cgcccgttga
    18781 acgctgaata tggtaaagtt gcaccaggtt ggggaacaac tccaattatg gctgtagtga
    18841 tgggtgcatt cttagtattt ttattaatca ttttacaaat ctataattca tcgttaatta
    18901 ttgaaaatgt agatgtagat tggacaaatg gtattgtgta ataccaaaaa aataatttaa
    18961 aaggttttag ttagaccttt taaattataa aacttaaata aattaaaaat ataaatctat
    19021 ggatattcta agtttagggt gggccgcttt aatgactatg tttacatttt cgttagcgtt
    19081 aacggtttgg gctagaaatg gcttctaatt atttaaactt tttataacat tttaaattga
    19141 aaatattatg gaattaataa aacaaatatt cccgtttgca aattcggaaa ttattacagc
    19201 agcagttgta tgtttctcta tgactttatt cggtctttca ctaggcttcg gtttgttaaa
    19261 agttcaaggt gaataaaaat tcatttaata ataatattta ttatattatt attaaatttg
    19321 aggttaaaat cgggactgac gagactcgaa ctcgcaactt ccgccgtgac agggcggtgc
    19381 tctaaccaat tgaactacaa tcccagttat gagtatgaat gatgtaacct ataacatact
    19441 agattttctt tgacctgtca actattttat attttattta atcgttagaa aggagaaaca
    19501 gggattcgaa ccctgggtac aaaaaattgt acgatagatt agcaatctat tgctttcgac
    19561 cactcagcca tttctcccga actaagaaac taaataacaa gcttattttt ttctaaaatt
    19621 tagtttaaaa gtagatggct ctggtgggat ttgaacctac gaccttgggc ttatgagtcc
    19681 cctgctctaa ccactgagct acagagcctt atatcactct actacaatag attctatcat
    19741 agattaaaca aaatatagta ttaatttagc taatctatgg taaaataaaa tttaatgaat
    19801 acttaaaact atacacaata tattaaaact taatggctgt aaattttata tgagtgattt
    19861 ctggactaat atttcacgtt atcctagatt ttttattagt agtttagctg gctcaatttt
    19921 aattatccta acaccgttta aaaatttatt aaaaattcaa aaatttcaaa ttttagtccc
    19981 cattttactg ttgagttttg taattttact agcttttatt cttattaaca tgactgcaat
    20041 ctaaaaaagt tttcaatttc tgagtaaaaa ttaatacttc tttcataaaa tttgaaattg
    20101 ggccgagttg gattcgaacc aacgtagcgt gacgcagcgg atttacaatc cgcccccttt
    20161 aaccactcgg gcaccgaccc atgttgaaga gtattctaac acaattaaga ttaaaaaaca
    20221 atattagaat tttaataaaa acatttttag aattctgata ttgacgttag ttttattttt
    20281 tcgtatattt ctgggtgtgc atagctaact agtatatgta ttgaataatg ggtaattaac
    20341 tcagcggtag agtgtctgcc ttacaagcag aaggtcacag gttcaaatcc tgtattaccc
    20401 atattttagg gcctatcgtc taacggatta ggacagaaac cttctaagtt tccaatgtag
    20461 gttcgattcc tactgggcct attaacaaat ttaaacaaat ttttattata atttgactct
    20521 ctattttaag attgtttaga cacattggcc ggctaatgaa tttacacttg ttaccaatac
    20581 taatgactaa tggacactcg actaagataa actaaactct cattttaatt tgtttgatct
    20641 cttaattttt actcgagata aacaagttct tagattgaaa aactatttag aataaaaatt
    20701 aaatatcaaa agctggtgaa gggatttgaa cccccaacct acggattaca aatccgttgc
    20761 tctaccattg agctacacca gcacttctac accgtattat actataccat actatttcca
    20821 gtctagtctt taatttaaat aaaaaagatt tttaaaatgg gtattaaaat cttacaaatt
    20881 tgtaagattt taaccgacta aatttcaaat tgattaccac gacgatattg caaaaatgct
    20941 gcaacaaata aaccaagtgc cgatacagtg atcaatccca aaacgatacc agataataaa
    21001 ggttctacca ttgattgtta tcctttaatt ttaatgtaaa cgtgaatact gtcctataaa
    21061 tgattataca gtaacaagtt tctattagaa agttaaaatt atctaaaccc aatagcagct
    21121 tgccatacaa aagctaataa taagaaaaag acagggatga ttggtagcac atctacaatg
    21181 ggactaaaaa caacatatgc ttctggcaaa cgagacaata ataaagtttc cataagtaaa
    21241 gatattatat ttatatataa attttcgaag attaataata ttaattttag tataaaactg
    21301 ttgaaaattt taatttatat taatcttttg attctattct tttttcatat acaattcata
    21361 gtatcaattt cttaaattga tatatatata taaattaaaa tttctttaaa aataaaagta
    21421 tggcagcttc atttctacct gcaattttag ttcccattgt tggtttagtt tttccagctt
    21481 taagcatggc attatttttc atctacgctt caattgatga cattagttag atagtttttt
    21541 agattctcag tacgaacaag taatgagaat ctaaaaatat ttattctaaa attttttata
    21601 atttctattt tttcaataat aaataaaaat tataaactac ttagaaaaga attaatgtat
    21661 aatggtgatt acaagttgat aagaccagaa tagaattttt atttgtctat tctataaata
    21721 atttaattaa aggattatta aaatatgacc attgctattg ggcaaaacca agaacgtggc
    21781 atttttgatc taattgacga ttggttaaag aaagatcgat ttgttttcgt tggttggtca
    21841 ggtttattat tattcccgac agcgtattta gctgttggtg gttggatgac tggtacaaca
    21901 tttgttacat cgtggtacac tcatggttta gcaagttcat atcttgaagg atgtaacttc
    21961 ttaactgctg ctgtttcgag tccagctaac agtatgggtc attcgttatt gcttttatgg
    22021 ggccctgaag cgcaaggtga tttcacacgt tggtgtcaaa tcggtggtct ttgaagagcg
    22081 ggcttcgccc tgtcgctcga ctgcggcgag cactactggc tgtaaaagga cagaccacat
    22141 catggttctg tgttcattag gttgttctgt ccattgctga cataatccgc tccacttcaa
    22201 cgtaacaccg cacgaagatt tctattgttc ctgaaggcat attcaaatcg ttttcgttac
    22261 cgcttgcagg catcatgaca gaacactact tcctataaac gctacacagg ctcctgagat
    22321 taataatgcg gatctctacg ataatgggag attttcccga ctgtttcgtt cgcttctcag
    22381 tggataacag ccagcttctc tgtttaacag acaaaaacag catatccact cagttccaca
    22441 tttccatata aaggccaagg catttattct caggataatt gtttcagcat cgcaaccgca
    22501 tcagactccg gcatcgcaaa ctgcacccgg tgccgggcag ccacatccag cgcaaaaacc
    22561 ttcgtgtaga cttccgttga actgatggac ttatgtccca tcaggctttg cagaactttc
    22621 agcggtatac cggcatacag catgtgcatc gcataggaat ggcggaacgt atgtggtgtg
    22681 accggaacag agaacgtcac accgtcagca gcagcggcgg caaccgcctc cccaatccag
    22741 gtcctgaccg ttctgtccgt cacttcccag atccgcgctt tctctgtcct tcctgtgcga
    22801 cggttacgcc gctccatgag cttatcgcga ataaatacct gtgacggaag atcacttcgc
    22861 agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca acttttggcg
    22921 aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc
    22981 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga
    23041 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
    23101 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
    23161 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
    23221 tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct
    23281 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
    23341 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
    23401 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
    23461 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
    23521 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa
    23581 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg
    23641 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt
    23701 ttaaggcagt tattggtgcc cttaaacgcc tggttgctac gcctgaataa gtgataataa
    23761 gcggatgaat ggcagaaatt cgatgataag ctgtcaaaca tgagaattgg tcgacggccc
    23821 gggcggccgc aaggggttcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    23881 ccgactagaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    23941 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    24001 aacaatttca cacaggaaac agctatgacc atgattacgc caagctattt aggtgagact
    24061 atagaatact caagcttgca tgcctgcagg tcgactctag aggatccccg ggtaccgagc
    24121 tcgaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt
    24181 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
    24241 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagctga
    24301 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgtggt atttcacacc
    24361 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
    24421 acccgccaac acccgctgac gcgaacccct tgcggccgca tcgaatataa cttcgtataa
    24481 tgtatgctat acgaagttat tagcgatgag ctcggacttc cattgttcat tccacggaca
    24541 aaaacagaga aaggaaacga cagaggccaa aaagctcgct ttcagcacct gtcgtttcct
    24601 ttcttttcag agggtatttt aaataaaaac attaagttat gacgaagaag aacggaaacg
    24661 ccttaaaccg gaaaattttc ataaatagcg aaaacccgcg aggtcgccgc cccgtaacct
    24721 gtcggatcac cggaaaggac ccgtaaagtg ataatgatta tcatctacat atcacaacgt
    24781 gcgtggaggc catcaaacca cgtcaaataa tcaattatga cgcaggtatc gtattaattg
    24841 atctgcatca acttaacgta aaaacaactt cagacaatac aaatcagcga cactgaatac
    24901 ggggcaacct catgtccgag ctcgcgagct cgtcgacagc gacacacttg catcggatgc
    24961 agcccggtta acgtgccggc acggcctggg taaccaggta ttttgtccac ataaccgtgc
    25021 gcaaaatgtt gtggataagc aggacacagc agcaatccac agcaggcata caaccgcaca
    25081 ccgaggttac tccgttctac aggttacgac gacatgtcaa tacttgccct tgacaggcat
    25141 tgatggaatc gtagtctcac gctgatagtc tgatcgacaa tacaagtggg accgtggtcc
    25201 cagaccgata atcagaccga caacacgagt tggatcgtgg tcccagacta ataatcagac
    25261 cgacgatacg agtgggaccg tggtcccaga ctaataatca gaccgacgat acgagtggga
    25321 ccgtggttcc agactaataa tcagaccgac gatacgagtg ggaccgtggt cccagactaa
    25381 taatcagacc gacgatacga gtgggaccat ggtcccatac taataatcat accgacgata
    25441 cgagtgggac cgtggtccca gtctgattat cagaccgacg atacgagtgg gaccgtggtc
    25501 ccagactaat aatcagaccg acgatacgag tgggaccgtg gtcccagact aataatcaga
    25561 ccgacgatac gagtgggacc gtggtcccag tctgattatc agaccgacga tacaagtgga
    25621 acagtgggcc cagagagaat attcaggcca gttatgcttt ctggcctgta acaaaggaca
    25681 ttaagtaaag acagataaac gtagactaaa acgaggtcgc atcagggtgc tggcttttca
    25741 agttccttaa gaatggcctc aattttctct atacactcag ttggaacacg agacctgtcc
    25801 aggttaagca ccattttatc gcccttatac aatactgtcg ctccaggagc aaactgatgt
    25861 cgtgagctta aactagttct tgatgcagat gacgttttaa gcacagaagt taaaagagtg
    25921 ataacttctt cagcttcaaa tatcacccca gcttttttct gctcatgaag gttagatgcc
    25981 tgctgcttaa gtaattcctc tttatctgta aaggcttttt gaagtgcatc acctgaccgg
    26041 gcagatagtt caccggggtg agaaaaaaga gcaacaactg atttaggcaa tttggcggtg
    26101 ttgatacagc gggtaataat cttacgtgaa atattttccg catcagccag cgcagaaata
    26161 tttccagcaa attcattctg caatcggctt gcataacgct gaccacgttc ataagcactt
    26221 gttgggcgat aatcgttacc caatctggat aatgcagcca tctgctcatc atccagctcg
    26281 ccaaccagaa cacgataatc actttcggta agtgcagcag ctttacgacg gcgactccca
    26341 tcggcaattt ctatgacacc agatactctt cgaccgaacg ccggtgtctg ttgaccagtc
    26401 agtagaaaag aagggatgag atcatccagt gcgtcctcag taagcagctc ctggtcacgt
    26461 tcattacctg accatacccg agaggtcttc tcaacactat caccccggag cacttcaaga
    26521 gtaaacttca catcccgacc acatacaggc aaagtaatgg cattaccgcg agccattact
    26581 cctacgcgcg caattaacga atccaccatc ggggcagctg gtgtcgataa cgaagtatct
    26641 tcaaccggtt gagtattgag cgtatgtttt ggaataacag gcgcacgctt cattatctaa
    26701 tctcccagcg tggtttaatc agacgatcga aaatttcatt gcagacaggt tcccaaatag
    26761 aaagagcatt tctccaggca ccagttgatg agcgttgatc aatggcctgt tcaaaaacag
    26821 ttctcatccg gatctgacct ttaccaactt catccgtttc acgtacaaca ttttttagaa
    26881 ccatgcttcc ccaggcatcc cgaatttgct cctccatcca cggggactga gagccattgc
    26941 tattgctgta tttggtaagc aaaatacgta catcaggctc gaacccttta agatcaacgt
    27001 tcttgagcag atcacgaagc atatcg
//
