LOCUS       sequence-419966-        2949 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Level 0 Part: E. coli shuttle part (ampR_B0017) for the 
            construction of Level 1 plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Li X, Schonberg PY, Wucherpfennig T, Hinze C, Sulaj F, Henle T, 
            Mascher T
  TITLE     Development of a Golden Gate Assembly-Based Genetic Toolbox for 
            Lactiplantibacillus plantarum and Its Application for Engineering 
            Monoterpenoid Biosynthesis.
  JOURNAL   ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 
            10.1021/acssynbio.4c00075. Epub 2024 Sep 10.
  PUBMED    39254046
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00075"; journalName: "ACS Synth Biol"; date: 
            "2024-09-20- 20"; volume: "13"; issue: "9"; pages: "2764-2779"
COMMENT     Sequence Label: pLPE2
FEATURES             Location/Qualifiers
     source          1..2949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(96..684)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(176..195)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(930..998,999..1790)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1148..1167)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      1829..1900
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             2078..2089
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      2803..2874
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2890..2917
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
       61 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      121 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      181 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      241 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      301 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      361 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      421 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      481 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
      541 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      601 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      661 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      721 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      781 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
      841 acttggtctg acagctcgag gcttggggtc tcgggtccat gagacaataa ccctgataaa
      901 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      961 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     1021 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     1081 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     1141 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     1201 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     1261 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     1321 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     1381 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     1441 taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac
     1501 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     1561 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     1621 ataaatctgg agccggtgag cgtggttctc gcggtatcat tgcagcactg gggccagatg
     1681 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     1741 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     1801 aagtttacga agacgttcct ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1861 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagtaac atgagacccg
     1921 gaaagtgaaa cgtgatttca tgcgtcattt tgaacatttt gtaaatctta tttaataatg
     1981 tgtgcggcaa ttcacattta atttatgaat gttttcttaa catcgcggcc atcgcggcaa
     2041 ctcaagaaac ggcaggttcg gatcttagct actagagaaa gaggagaaat actagatggt
     2101 ttctaaaggt gaagcagtga tcaaagaatt tatgcgcttc aaagttcaca tggaaggttc
     2161 tatgaacggc cacgaatttg aaattgaagg tgaaggcgaa ggtcgtccat acgaaggtac
     2221 tcaaaccgca aaactgaaag ttaccaaagg tggtccactg ccattttctt gggatattct
     2281 gtctccacaa tttatgtacg gttctcgtgc atttatcaaa cacccagcag atattccaga
     2341 ctactacaaa caatcttttc cggaaggttt caaatgggaa cgtgttatga attttgaaga
     2401 tggtggtgca gttacggtta cccaagatac ctctctggaa gatggtactc tgatctacaa
     2461 agttaaactg cgtggtacta actttccacc agatggtcca gttatgcaga aaaaaaccat
     2521 gggttgggaa gcatctaccg aacgtctgta cccagaagat ggcgttctga aaggtgatat
     2581 caaaatggca ctgcgtctga aagatggcgg tcgttacctg gcagatttca aaaccaccta
     2641 caaagcgaaa aaaccagttc aaatgccagg tgcatacaac gttgatcgta aactggatat
     2701 taccagccac aacgaagatt acaccgttgt tgaacaatac gaacgttctg aaggccgtca
     2761 ctctaccggt ggtatggatg aactgtacaa ataaccaggc atcaaataaa acgaaaggct
     2821 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     2881 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atacagctca ctcaaaggcg
     2941 gtaatacgg
//
