LOCUS       sequence-419973-       27025 bp    DNA     circular UNA 15-DEC-2025
DEFINITION  A plasmid containing a 17,271 bp fragment of the P. tricornutum 
            chloroplast genome. The yeast marker URA3 was domesticated for SapI 
            and integrated into the chloroplast fragment..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 27025)
  AUTHORS   Walker EJL, Pampuch M, Chang N, Cochrane RR, Karas BJ
  TITLE     Design and assembly of the 117-kb Phaeodactylum tricornutum 
            chloroplast genome.
  JOURNAL   Plant Physiol. 2023 Dec 19:kiad670. doi: 10.1093/plphys/kiad670.
  PUBMED    38114089
REFERENCE   2  (bases 1 to 27025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 Dec 19:kiad670. doi: 10.1093/plphys/kiad670."
COMMENT     Sequence Label: pCHAP3_Sap
FEATURES             Location/Qualifiers
     source          1..27025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..756
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    759..1009
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     misc_feature    1171..1674
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        1728..1875
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             1876..2550
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     CDS             2946..4121
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             4121..5092
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    5165..5638
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    5898..6296
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(6314..6347)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     6415..6434
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(6514..6789)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGETIEYSSLHACRSTLEDPRVPSSNSPYSESYYNSL
                     AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS"
     primer_bind     6627..6649
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6641..6658
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6642..6658
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6665..6684
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6665..6683
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    6686..6742
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(6785..6801)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6785..6801)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6798..6820)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6809..6825
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6833..6839,6840..6857,6858..6863))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6878..6899
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(7119..7778)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7693..7712
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(7779..7881)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        14245..14460
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             14461..15264
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCCQQN"
     rep_origin      26026..26640
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      26117..26648
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      26716..26935
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
ORIGIN
        1 atggcggaaa cagcggttat caatcacaag aaacgtaaaa atagcccgcg aatcgtccag
       61 tcaaacgacc tcactgaggc ggcatatagt ctctcccggg atcaaaaacg tatgctgtat
      121 ctgttcgttg accagatcag aaaatctgat ggcaccctac aggaacatga cggtatctgc
      181 gagatccatg ttgctaaata tgctgaaata ttcggattga cctctgcgga agccagtaag
      241 gatatacggc aggcattgaa gagtttcgcg gggaaggaag tggtttttta tcgccctgaa
      301 gaggatgccg gcgatgaaaa aggctatgaa tcttttcctt ggtttatcaa acgtgcgcac
      361 agtccatcca gagggcttta cagtgtacat atcaacccat atctcattcc cttctttatc
      421 gggttacaga accggtttac gcagtttcgg cttagtgaaa caaaagaaat caccaatccg
      481 tatgccatgc gtttatacga atccctgtgt cagtatcgta agccggatgg ctcaggcatc
      541 gtctctctga aaatcgactg gatcatagag cgttaccagc tgcctcaaag ttaccagcgt
      601 atgcctgact tccgccgccg cttcctgcag gtctgtgtta atgagatcaa cagcagaact
      661 ccaatgcgcc tctcatacat tgagaaaaag aaaggccgcc agacgactca tatcgtattt
      721 tccttccgcg atatcacttc catgacgaca ggatagtctg agggttatct gtcacagatt
      781 tgagggtggt tcgtcacatt tgttctgacc tactgagggt aatttgtcac agttttgctg
      841 tttccttcag cctgcatgga ttttctcata ctttttgaac tgtaattttt aaggaagcca
      901 aatttgaggg cagtttgtca cagttgattt ccttctcttt cccttcgtca tgtgacctga
      961 tatcgggggt tagttcgtca tcattgatga gggttgatta tcacagttta ttactctgaa
     1021 ttggctatcc gcgtgtgtac ctctacctgg agtttttccc acggtggata tttcttcttg
     1081 cgctgagcgt aagagctatc tgacagaaca gttcttcttt gcttcctcgc cagttcgctc
     1141 gctatgctcg gttacacggc tgcggcgagc atcacgtgct ataaaaataa ttataattta
     1201 aattttttaa tataaatata taaattaaaa atagaaagta aaaaaagaaa ttaaagaaaa
     1261 aatagttttt gttttccgaa gatgtaaaag actctagggg gatcgccaac aaatactacc
     1321 ttttaccttg ctctacctgc tctcaggtat taatgccgaa ttgtttcatc ttgtctgtgt
     1381 agaagaccac acacgaaaat cctgtgattt tacattttac ttatcgttaa tcgaatgtat
     1441 atctatttaa tctgcttttc ttgtctaata aatatatatg taaagtacgc tttttgttga
     1501 aattttttaa acctttgttt attttttttt cttcattccg taactcttct accttcttta
     1561 tttactttct aaaatccaaa tacaaaacat aaaaataaat aaacacagag taaattccca
     1621 aattattcca tcattaaaag atacgaggcg cgtgtaagtt acaggcaagc gatccagatg
     1681 gcagtagtgg aagatattct ttattgaaaa atagcttgtc accttacgta caatcttgat
     1741 ccggagcttt tctttttttg ccgattaaga attaattcgg tcgaaaaaag aaaaggagag
     1801 ggccaagagg gagggcattg gtgactattg agcacgtgag tatacgtgat taagcacaca
     1861 aaggcagctt ggagtatgtc tgttattaat ttcacaggta gttctggtcc attggtgaaa
     1921 gtttgcggct tgcagagcac agaggccgca gaatgtgctc tagattccga tgctgacttg
     1981 ctgggtatta tatgtgtgcc caatagaaag agaacaattg acccggttat tgcaaggaaa
     2041 atttcaagtc ttgtaaaagc atataaaaat agttcaggca ctccgaaata cttggttggc
     2101 gtgtttcgta atcaacctaa ggaggatgtt ttggctctgg tcaatgatta cggcattgat
     2161 atcgtccaac tgcatggaga tgagtcgtgg caagaatacc aagagttcct cggtttgcca
     2221 gttattaaaa gactcgtatt tccaaaagac tgcaacatac tactcagtgc agcttcacag
     2281 aaacctcatt cgtttattcc cttgtttgat tcagaagcag gtgggacagg tgaacttttg
     2341 gattggaact cgatttctga ctgggttgga aggcaagaga gccccgaaag cttacatttt
     2401 atgttagctg gtggactgac gccagaaaat gttggtgatg cgcttagatt aaatggcgtt
     2461 attggtgttg atgtaagcgg aggtgtggag acaaatggtg taaaagactc taacaaaata
     2521 gcaaatttcg tcaaaaatgc taagaaatag gttattactg agtagtattt atttaagtat
     2581 tgtttgtgca cttgccgatc tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     2641 taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     2701 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcatttt
     2761 acacaggagt ctggacttga cgctagtgat aataagtgac tgaggtatgt gcgcttctta
     2821 tctccttttg tagtgttgct cttattttaa acaactttgc ggttttttga tgactttgcg
     2881 attttgttgt tgctttgcag taaattgcaa gatttaataa aaaaacgcaa agcaatgatt
     2941 aaaggatgtt cagaatgaaa ctcatggaaa cacttaacca gtgcataaac gctggtcatg
     3001 aaatgacgaa ggctatcgcc attgcacagt ttaatgatga cagcccggaa gcgaggaaaa
     3061 taacccggcg ctggagaata ggtgaagcag cggatttagt tggggtttct tctcaggcta
     3121 tcagagatgc cgagaaagca gggcgactac cgcacccgga tatggaaatt cgaggacggg
     3181 ttgagcaacg tgttggttat acaattgaac aaattaatca tatgcgtgat gtgtttggta
     3241 cgcgattgcg acgtgctgaa gacgtatttc caccggtgat cggggttgct gcccataaag
     3301 gtggcgttta caaaacctca gtttctgttc atcttgctca ggatctggct ctgaaggggc
     3361 tacgtgtttt gctcgtggaa ggtaacgacc cccagggaac agcctcaatg tatcacggat
     3421 gggtaccaga tcttcatatt catgcagaag acactctcct gcctttctat cttggggaaa
     3481 aggacgatgt cacttatgca ataaagccca cttgctggcc ggggcttgac attattcctt
     3541 cctgtctggc tctgcaccgt attgaaactg agttaatggg caaatttgat gaaggtaaac
     3601 tgcccaccga tccacacctg atgctccgac tggccattga aactgttgct catgactatg
     3661 atgtcatagt tattgacagc gcgcctaacc tgggtatcgg cacgattaat gtcgtatgtg
     3721 ctgctgatgt gctgattgtt cccacgcctg ctgagttgtt tgactacacc tccgcactgc
     3781 agtttttcga tatgcttcgt gatctgctca agaacgttga tcttaaaggg ttcgagcctg
     3841 atgtacgtat tttgcttacc aaatacagca atagcaatgg ctctcagtcc ccgtggatgg
     3901 aggagcaaat tcgggatgcc tggggaagca tggttctaaa aaatgttgta cgtgaaacgg
     3961 atgaagttgg taaaggtcag atccggatga gaactgtttt tgaacaggcc attgatcaac
     4021 gctcatcaac tggtgcctgg agaaatgctc tttctatttg ggaacctgtc tgcaatgaaa
     4081 ttttcgatcg tctgattaaa ccacgctggg agattagata atgaagcgtg cgcctgttat
     4141 tccaaaacat acgctcaata ctcaaccggt tgaagatact tcgttatcga caccagctgc
     4201 cccgatggtg gattcgttaa ttgcgcgcgt aggagtaatg gctcgcggta atgccattac
     4261 tttgcctgta tgtggtcggg atgtgaagtt tactcttgaa gtgctccggg gtgatagtgt
     4321 tgagaagacc tctcgggtat ggtcaggtaa tgaacgtgac caggagctgc ttactgagga
     4381 cgcactggat gatctcatcc cttcttttct actgactggt caacagacac cggcgttcgg
     4441 tcgaagagta tctggtgtca tagaaattgc cgatgggagt cgccgtcgta aagctgctgc
     4501 acttaccgaa agtgattatc gtgttctggt tggcgagctg gatgatgagc agatggctgc
     4561 attatccaga ttgggtaacg attatcgccc aacaagtgct tatgaacgtg gtcagcgtta
     4621 tgcaagccga ttgcagaatg aatttgctgg aaatatttct gcgctggctg atgcggaaaa
     4681 tatttcacgt aagattatta cccgctgtat caacaccgcc aaattgccta aatcagttgt
     4741 tgctcttttt tctcaccccg gtgaactatc tgcccggtca ggtgatgcac ttcaaaaagc
     4801 ctttacagat aaagaggaat tacttaagca gcaggcatct aaccttcatg agcagaaaaa
     4861 agctggggtg atatttgaag ctgaagaagt tatcactctt ttaacttctg tgcttaaaac
     4921 gtcatctgca tcaagaacta gtttaagctc acgacatcag tttgctcctg gagcgacagt
     4981 attgtataag ggcgataaaa tggtgcttaa cctggacagg tctcgtgttc caactgagtg
     5041 tatagagaaa attgaggcca ttcttaagga acttgaaaag ccagcaccct gatgcgacct
     5101 cgttttagtc tacgtttatc tgtctttact taatgtcctt tgttacaggc cagaaagcat
     5161 aactggcctg aatattatct ctgggcccac tgttccactt gtatcgtcgg tctgataatc
     5221 agactgggac cacggtccca ctcgtatcgt cggtctgatt attagtctgg gaccacggtc
     5281 ccactcgtat cgtcggtctg attattagtc tgggaccacg gtcccactcg tatcgtcggt
     5341 ctgataatca gactgggacc acggtcccac tcgtatcgtc ggtatgatta ttagtatggg
     5401 accatggtcc cactcgtatc gtcggtctga ttattagtct gggaccacgg tcccactcgt
     5461 atcgtcggtc tgattattag tctggaacca cggtcccact cgtatcgtcg gtctgattat
     5521 tagtctggga ccacggtccc actcgtatcg tcggtctgat tattagtctg ggaccacgat
     5581 ccaactcgtg ttgtcggtct gattatcggt ctgggaccac ggtcccactt gtattgtcga
     5641 tcagactatc agcgtgagac tacgattcca tcaatgcctg tcaagggcaa gtattgacat
     5701 gtcgtcgtaa cctgtagaac ggagtaacct cggtgtgcgg ttgtatgcct gctgtggatt
     5761 gctgctgtgt cctgcttatc cacaacattt tgcgcacggt tatgtggaca aaatacctgg
     5821 ttacccaggc cgtgccggca cgttaaccgg gctgcatccg atgcaagtgt gtcgctgtcg
     5881 acgagctcgc gagctcggac atgaggttgc cccgtattca gtgtcgctga tttgtattgt
     5941 ctgaagttgt ttttacgtta agttgatgca gatcaattaa tacgatacct gcgtcataat
     6001 tgattatttg acgtggtttg atggcctcca cgcacgttgt gatatgtaga tgataatcat
     6061 tatcacttta cgggtccttt ccggtgatcc gacaggttac ggggcggcga cctcgcgggt
     6121 tttcgctatt tatgaaaatt ttccggttta aggcgtttcc gttcttcttc gtcataactt
     6181 aatgttttta tttaaaatac cctctgaaaa gaaaggaaac gacaggtgct gaaagcgagc
     6241 tttttggcct ctgtcgtttc ctttctctgt ttttgtccgt ggaatgaaca atggaagtcc
     6301 gagctcatcg ctaataactt cgtatagcat acattatacg aagttatatt cgatgcggcc
     6361 gcaaggggtt cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     6421 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccacac agatgcgtaa
     6481 ggagaaaata ccgcatcagg cgccattcgc cattcagctg cgcaactgtt gggaagggcg
     6541 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     6601 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     6661 attgtaatac gactcactat agggcgaatt cgagctcggt acccggggat cctctagagt
     6721 cgacctgcag gcatgcaagc ttgagtattc tatagtctca cctaaatagc ttggcgtaat
     6781 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6841 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     6901 ttgcgttgcg ctcactgccc gctttctagt cgggaaacct gtcgtgccag ctgcattaat
     6961 gaatcggcca acgcgaaccc cttgcggccg cccgggccgt cgaccaattc tcatgtttga
     7021 cagcttatca tcgaatttct gccattcatc cgcttattat cacttattca ggcgtagcaa
     7081 ccaggcgttt aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca
     7141 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     7201 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     7261 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     7321 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa
     7381 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     7441 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     7501 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga
     7561 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     7621 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     7681 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     7741 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     7801 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
     7861 gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg
     7921 gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt
     7981 tattcgcgat aagctcatgg agcggcgtaa ccgtcgcaca ggaaggacag agaaagcgcg
     8041 gatctgggaa gtgacggaca gaacggtcag gacctggatt ggggaggcgg ttgccgccgc
     8101 tgctgctgac ggtgtgacgt tctctgttcc ggtcacacca catacgttcc gccattccta
     8161 tgcgatgcac atgctgtatg ccggtatacc gctgaaagtt ctgcaaagcc tgatgggaca
     8221 taagtccatc agttcaacgg aagtctacac gaaggttttt gcgctggatg tggctgcccg
     8281 gcaccgggtg cagtttgcga tgccggagtc tgatgcggtt gcgatgctga aacaattatc
     8341 ctgagaataa atgccttggc ctttatatgg aaatgtggaa ctgagtggat atgctgtttt
     8401 tgtctgttaa acagagaagc tggctgttat ccactgagaa gcgaacgaaa cagtcgggaa
     8461 aatctcccat tatcgtagag atccgcatta ttaatctcag gagcctgtgt agcgtttata
     8521 ggaagtagtg ttctgtcatg atgcctgcaa gcggtaacga aaacgatttg aatatgcctt
     8581 caggaacaat agaaatcttc gtgcggtgtt acgttgaagt ggagcggatt atgtcagcaa
     8641 tggacagaac aacctaatga acacagaacc atgatgtggt ctgtcctttt acagccagta
     8701 gtgctcgccg cagtcgagcg acagggcgaa gcccgctctt caaagaccac cgatttgaca
     8761 ccaacgtgtg aaatcacctt gcgcttcagg gccccataaa agcaataacg aatgacccat
     8821 actgttagct ggactcgaaa cagcagcagt taagaagtta catccttcaa gatatgaact
     8881 tgctaaacca tgagtgtacc acgatgtaac aaatgttgta ccagtcatcc aaccaccaac
     8941 agctaaatac gctgtcggga ataataataa acctgaccaa ccaacgaaaa caaatcgatc
     9001 tttctttaac caatcgtcaa ttagatcaaa aatgccacgt tcttggtttt gcccaatagc
     9061 aatggtcata ttttaataat cctttaatta aattatttat agaatagaca aataaaaatt
     9121 ctattctggt cttatcaact tgtaatcacc attatacatt aattcttttc taagtagttt
     9181 ataattttta tttattattg aaaaaataga aattataaaa aattttagaa taaatatttt
     9241 tagattctca ttacttgttc gtactgagaa tctaaaaaac tatctaacta atgtcatcaa
     9301 ttgaagcgta gatgaaaaat aatgccatgc ttaaagctgg aaaaactaaa ccaacaatgg
     9361 gaactaaaat tgcaggtaga aatgaagctg ccatactttt atttttaaag aaattttaat
     9421 ttatatatat atatcaattt aagaaattga tactatgaat tgtatatgaa aaaagaatag
     9481 aatcaaaaga ttaatataaa ttaaaatttt caacagtttt atactaaaat taatattatt
     9541 aatcttcgaa aatttatata taaatataat atctttactt atggaaactt tattattgtc
     9601 tcgtttgcca gaagcatatg ttgtttttag tcccattgta gatgtgctac caatcatccc
     9661 tgtctttttc ttattattag cttttgtatg gcaagctgct attgggttta gataatttta
     9721 actttctaat agaaacttgt tactgtataa tcatttatag gacagtattc acgtttacat
     9781 taaaattaaa ggataacaat caatggtaga acctttatta tctggtatcg ttttgggatt
     9841 gatcactgta tcggcacttg gtttatttgt tgcagcattt ttgcaatatc gtcgtggtaa
     9901 tcaatttgaa atttagtcgg ttaaaatctt acaaatttgt aagattttaa tacccatttt
     9961 aaaaatcttt tttatttaaa ttaaagacta gactggaaat agtatggtat agtataatac
    10021 ggtgtagaag tgctggtgta gctcaatggt agagcaacgg atttgtaatc cgtaggttgg
    10081 gggttcaaat cccttcacca gcttttgata tttaattttt attctaaata gtttttcaat
    10141 ctaagaactt gtttatctcg agtaaaaatt aagagatcaa acaaattaaa atgagagttt
    10201 agtttatctt agtcgagtgt ccattagtca ttagtattgg taacaagtgt aaattcatta
    10261 gccggccaat gtgtctaaac aatcttaaaa tagagagtca aattataata aaaatttgtt
    10321 taaatttgtt aataggccca gtaggaatcg aacctacatt ggaaacttag aaggtttctg
    10381 tcctaatccg ttagacgata ggccctaaaa tatgggtaat acaggatttg aacctgtgac
    10441 cttctgcttg taaggcagac actctaccgc tgagttaatt acccattatt caatacatat
    10501 actagttagc tatgcacacc cagaaatata cgaaaaaata aaactaacgt caatatcaga
    10561 attctaaaaa tgtttttatt aaaattctaa tattgttttt taatcttaat tgtgttagaa
    10621 tactcttcaa catgggtcgg tgcccgagtg gttaaagggg gcggattgta aatccgctgc
    10681 gtcacgctac gttggttcga atccaactcg gcccaatttc aaattttatg aaagaagtat
    10741 taatttttac tcagaaattg aaaacttttt tagattgcag tcatgttaat aagaataaaa
    10801 gctagtaaaa ttacaaaact caacagtaaa atggggacta aaatttgaaa tttttgaatt
    10861 tttaataaat ttttaaacgg tgttaggata attaaaattg agccagctaa actactaata
    10921 aaaaatctag gataacgtga aatattagtc cagaaatcac tcatataaaa tttacagcca
    10981 ttaagtttta atatattgtg tatagtttta agtattcatt aaattttatt ttaccataga
    11041 ttagctaaat taatactata ttttgtttaa tctatgatag aatctattgt agtagagtga
    11101 tataaggctc tgtagctcag tggttagagc aggggactca taagcccaag gtcgtaggtt
    11161 caaatcccac cagagccatc tacttttaaa ctaaatttta gaaaaaaata agcttgttat
    11221 ttagtttctt agttcgggag aaatggctga gtggtcgaaa gcaatagatt gctaatctat
    11281 cgtacaattt tttgtaccca gggttcgaat ccctgtttct cctttctaac gattaaataa
    11341 aatataaaat agttgacagg tcaaagaaaa tctagtatgt tataggttac atcattcata
    11401 ctcataactg ggattgtagt tcaattggtt agagcaccgc cctgtcacgg cggaagttgc
    11461 gagttcgagt ctcgtcagtc ccgattttaa cctcaaattt aataataata taataaatat
    11521 tattattaaa tgaattttta ttcaccttga acttttaaca aaccgaagcc tagtgaaaga
    11581 ccgaataaag tcatagagaa acatacaact gctgctgtaa taatttccga atttgcaaac
    11641 gggaatattt gttttattaa ttccataata ttttcaattt aaaatgttat aaaaagttta
    11701 aataattaga agccatttct agcccaaacc gttaacgcta acgaaaatgt aaacatagtc
    11761 attaaagcgg cccaccctaa acttagaata tccatagatt tatattttta atttatttaa
    11821 gttttataat ttaaaaggtc taactaaaac cttttaaatt atttttttgg tattacacaa
    11881 taccatttgt ccaatctaca tctacatttt caataattaa cgatgaatta tagatttgta
    11941 aaatgattaa taaaaatact aagaatgcac ccatcactac agccataatt ggagttgttc
    12001 cccaacctgg tgcaacttta ccatattcag cgttcaacgg gcgtaaaagt tcacctaatc
    12061 ttgttcgtaa tgccataaaa agatttattt tttaataaat taatttttct actatatgtt
    12121 atataatgtt aaactgttag ttttaatatt atataacatg gaaacagcaa ctattatcgt
    12181 aatttttgtc tcaagtctac tcttaggaat taccacatat tctgtttata cggcttttgg
    12241 acccgcttca aaaaatttac gtgatccatt cgaagaacat gaggattaaa aaaataaaac
    12301 cattttaatt ggttttattt tttaataatt cgaggtgttt ctctgaaaaa tacggcaaag
    12361 aaaattacga ttaaggtgcc aataagtaaa aatgtataaa ctaacgcttc cattgtttga
    12421 tcctatttta atataaagaa cttaaaagat taactgtatt ttatacagca ccctgttttt
    12481 tcgttgtttt atcaccaagt ttttggaata caccaaattc tacttgctcc gtaacttcag
    12541 caccaattcc agtaaatacg tcacggaaaa ttgttctacc accatgccat agatgtccaa
    12601 agaagaataa taaagcaaag ttagcatgac cataggtata ccaaccacgt ggactacttc
    12661 tgaacacacc atctgattct aaacttgttc tatcgaattc aaaaacttca cctaattgtg
    12721 atttacgagc aaacttttta acggttggtg catctttaaa tgtttgacca tttaatttac
    12781 caccgtaaaa atcaactgtt actcccactt gttcaatact atattttgat tctgcacgac
    12841 ggaatggaat atcagctcga ataataccat ctttatctac taaaattaca gggaatgttt
    12901 caaagaaagc tggcatacga cgtacagtta actcacgacc ttctttgtca cggaaaattg
    12961 gatgacctag ccaagcttct gcgataccat ctcctttatt catcggaccc gcacggaata
    13021 aaccgccttt agctgggtta ttaccaatat aatcataaaa ggctaattta tctggaattc
    13081 ttgaccatgc ttgactttcc gataaacctt cacttactga agcttcaact tgacgctcaa
    13141 tttcttgttg gaaataaccg ctatcccact gataacgtgt tgggccaaat aattcaattg
    13201 gtgttgcagc agctccatac cacattgtcc ctgatgttac aaacgctgca aagaaaacgg
    13261 ccgaaatact actagataaa actgtttcaa tattacccat tcttaaagcc cgatataaac
    13321 gttgtggtgg acgaactgtt aagtggaaaa cgcctgcaag aataccgaag agacctgctg
    13381 caatatggtg cgcagcaata ccacctgggt taaacgggtt aaatccgtct gcaccccacg
    13441 atggcgccac tggttgtact ttacctgtga ttccatacgc atctgaaacc cagataccag
    13501 gaccaaataa acctgttacg tggaatgcac cgaaaccaaa acataataaa cctgataaaa
    13561 ataaatgaat accaaagatt tttggtaaat ctaaagcagg ttcacctgtt cgtggatcac
    13621 ggaataattc taaatcccaa taaacccaat gccagattgc agctaaaaag cacatacctg
    13681 acaaaacaat atgcgataat gctacacctt cgaaactcca aagaccaggg ttagatacac
    13741 tttcaccagt aatactccaa ccaccccaag aatcagtaat acctagtctt gtcataaatg
    13801 gcataacaaa catcccttga cgccacatcg gatttaaaac tggatctgat ggatcgaata
    13861 cagctaattc gtataaggcc attgatccag cccaacctgc aaccaaagct gtatgcataa
    13921 tgtgaacagc aattaatctt cctggatcat ttaaaacaac agtgtgaaca cgataccatg
    13981 gtaatgccat agtaatagat tcctcaatat aataatggtt tttgactttc ttacaactaa
    14041 actacataaa agttagctat tataacatta taagccaaga tgaaaaaaaa ttaagataaa
    14101 aaatcaattt aattttgttt gagacaaatt ttgtatacca ataggcttga aaagtccaga
    14161 aaaactggcg cgcccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac
    14221 gcggcgcatc gggggggggg gggtttcaat tcatcatttt ttttttattc ttttttttga
    14281 tttcggtttc cttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg
    14341 aaggagcaca gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat
    14401 tgcccagtat tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc
    14461 atgtcgaaag ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag
    14521 ctatttaata tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc
    14581 accaaggaat tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca
    14641 catgtggata tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta
    14701 tccgccaagt acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca
    14761 gtcaaattgc agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat
    14821 gcacacggtg tggtgggccc aggtattgtt agcggtttga agcaggcggc ggaagaagta
    14881 acaaaggaac ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctagct
    14941 actggagaat atactaaggg tactgttgac attgcgaaaa gcgacaaaga ttttgttatc
    15001 ggctttattg ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg
    15061 acacccggtg tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg
    15121 gatgatgtgg tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag
    15181 ggaagggatg ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg
    15241 agaagatgct gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac
    15301 tcacaaatta gagcttcaat ttaattatat cagttattac ccggccggga atctcggtcg
    15361 taatgatttt tataatgacg aaaaaaaaaa aaattggaaa gaaaaccccc ccccccgcag
    15421 cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgaggc gcgccagagt
    15481 ttttaacttc taaattaacg taattctagt aataaattaa ctatgaattt tttgacttta
    15541 acttgctaaa atttttaata tagaataaga ttaactcaac actgaccctt attaaatttc
    15601 caaaaattct ctattcaaac aaaaaactat taattatggc aaagaaaagt atgatagagc
    15661 gtgagaaaaa gcgtattaaa ctaaccaaga aatatgcttt gaaacgtgca actttattaa
    15721 aaaaatacca aactgaagaa agttttaact taaaattgga acttcattca aaaattcaga
    15781 agttaccacg caatagtgca aaaacaagaa ttcgaaatcg atgttggaaa acaggacgtc
    15841 cacgtggagt ttttcgtgat tttggtttat cacgtcacgt ttttcgtgaa atggctcatc
    15901 aatgcttatt accaggtgta acaaaatcaa gttggtaaaa aaatttactt aaattaaatt
    15961 aaatgatttt attaatttaa tttacaatta catatacgaa gttaaagtat ttgtagaaat
    16021 attttattat catttaggat atttaattct ttagaatcaa aacaaattta attggaaata
    16081 aggagttttt gatgatttac gattcacaaa tttatacagt gctatgtatt gcgttattag
    16141 ccggaatttt agcgattaga ttaggttcga cattatatga ataattgttc tttaaattat
    16201 taaaaagaaa ttattatttt aaaaaccgtt tacattaata atcgataata agttatggta
    16261 accaaagatt taaaaaaatt tactactccc gtatttcctt ttacagcaat tgttggccaa
    16321 gaagaaatga aattggcttt acaattaaac gtaattgatc caaaaattgg tggagttatg
    16381 attatggggg atcgaggaac aggaaaatca acaacaattc gcgcaattgc tgatttactt
    16441 ccagaaattg aagtagttaa agatgatcca ttcaattcac ataaatcaga tcttgattta
    16501 atgggaaacg aagttaaatc agcaattcag aacgatgaag ctattgaaac ggaatttatc
    16561 aagattccaa tggttgattt gcctctggga gcaactgaag atagagtatg tgggactatt
    16621 gatattgaaa aagctttaac tgaaggtata aaagcttttg aaccaggtct attagctaaa
    16681 gcaaatagag gactacttta cgtagacgaa gtaaacttat tagacgatca tcttgtcgat
    16741 attttattag attctgcagc atctggttgg aacacagttg aacgcgaagg catttcaatt
    16801 cgtcacccag cacgttttgt tttagttgga tcaggaaatc ccgaggaagg cgagttacgt
    16861 cctcagttac tcgatcgttt cggtatgcac gctgagatta gaaccgtaaa agatcctatg
    16921 ttacgtgtta aagttgtaga agaaagaaca tcatttgacc aaagtccaat ggtatggatt
    16981 gaaaattacg aaattcaaca acaagaatta cgcgaccgaa tcgtagccgc tcaaaaatta
    17041 cttccaacag ttgaaattga ttacgattta cgggttaaaa tttcagaagt atgtagtcgc
    17101 ttagatgtag atggactacg tggtgatatt gtaacaaatc gggctgcaaa agctcatgca
    17161 gcatatcata aacgtgataa agttactata aatgatattg ctagtattat tactttatgc
    17221 ttacgtcata gactacgaaa agatccatta gaaacaattg attcaggtga taaagtgggt
    17281 aaggtattta gagagatctt tgaaattgag gattaaaact actttagatt tttataaata
    17341 gttagtcatt catagtgtaa aatttttaaa ttagtccaaa aatatgttaa aactatttgc
    17401 atttgctatc agtctatttt taattgtcat tattttttta cgaatcccac aagaaaatgt
    17461 cggattagca agttttgcta caaaaagcga actattgggc tcacctagtt cagcccaacg
    17521 atcgttaaat attttaactg gttttggtat atttatttat tttgggattg ctatccaaat
    17581 gaatttacta aatcattaaa atttagacaa aagattttga tcactacaaa ttaatagata
    17641 gtgatcaaaa tcttttgttt accaaaaacc taaggaaata gctttgtcaa ttggtacaca
    17701 tgcacctata cctaaccaaa ctgctgtaaa aaacccaaaa ataaaagtta gactcgcaag
    17761 cgggcgacga aacggatttt gaaatttatt tacgttttca ataaatggaa ccgtgattaa
    17821 tcctaaagga actgctgcca ttgctaaaac tcctaataat ttattcggta acacgcgtaa
    17881 taaattaaat gtcgggaaaa aataccattc tggtaaaatt tctaatggtg tattaaatgg
    17941 atcagctggt tcacctagtt gagtcggtgc taaaactgat aaacctacaa tgcatgttaa
    18001 tgttcctaac atcgtaagtg ggaagacata taaaagatca tttggccatg ctggttctcc
    18061 ataatagtta tggcccatac ctttagctaa tttagcacgt aattttggat cagttaaatc
    18121 tggtttttta atgactgaca taatagaatt tattaataat tattttataa ttggttaaaa
    18181 atttaatgtt caaaatttct ttaaaacacc tttataatgg gcctgagata ccttgtttgc
    18241 gaatcattaa gaaatgagtt agcattaata ctgctgccgc caatggtaaa acaaaagtat
    18301 gagcactata gaaacgtgtt aatgtacttt gacctacact ttctccacca cgtaagatta
    18361 aaactaaagg ttcacctaca attggaactg ccgctggtac accagttaca attttacacg
    18421 cccaaaatcc tacctgatcc catggtaatg aataacctgt tacaccaaat gaaacggtaa
    18481 caacggataa agtgacacct gtgacccaag tcaattcacg tggtttttta aatccaccag
    18541 ttaaataaac tctaaaaacg tgtaacacca tcatcaacac catcatactt gctgaccaac
    18601 gatggattga gcgaattaac caaccaaaat tgacactcgt cataatatat tctactgatg
    18661 caaatgcgtc aacaacacta ggtcgataat agaatgtcat tgcaaaacca gttgcaactt
    18721 gaattaaaaa acacgttaaa acaagacctc caaaacaata aaaaatattt acatgaggtg
    18781 gtacatattt actagaaata tcatcagcaa ttgcttgaac ttctagacgt tcttcgaacc
    18841 aatcgtaaac tttattcata aaaattttca gaaaattttg acacggttaa gcaaaacaat
    18901 tttaaaaaat aaaggcgaga gtgggattcg aacccacgga acctgtaaag attcatctga
    18961 tttcaaatca gacgcaatcg accaactctg ccatctcgcc aaaagattag taacaaaact
    19021 aatcttagat attaactaac tttcatacgt atttattcca ctaaacttaa tagaagctgg
    19081 atttggattt tttccaatcg aaaaatcgcg gttattaacg gcagttcgac ctggatttac
    19141 tttttcagga aaaactccat cttttggatg taaatattga acttctccac ttgggaaaat
    19201 tcgataaatt ttataatcat taattttaaa tgtacgtaat tgggttccca atgctaaaca
    19261 ttgctctttg cgcgctaagt ataataaatt ttctccacta cgcatgatag ctgctccacc
    19321 aattggcatc tcaaatattt gctctttggg gcttgtccaa gtaattgcgt atttttcttc
    19381 aacttcagct gatcgtaacc agcctccagt acttccacca aaagtaggaa acggcgtttg
    19441 taaatttaat gtcatctgta aaactttata aatgtttaac gttcactata taattttaat
    19501 aaaaaaaaaa attttttatt aaaattatga aaatttctag cggagaatgg atttgaacca
    19561 ttgaccttcg ggttatgagc ccaacgagct accaggctgc tctactccgc gacagataaa
    19621 aaattacaaa atcacattta gatccagact taacaaccgg tatatatatt atttccttag
    19681 agtatcaaca tataaacaaa ttataacata tcttcatagt cgctgtcaac taacgtgtta
    19741 ataaattaac caaaattttc aatcatggtt aatttattaa tcatcaaaaa ctaaatttaa
    19801 aattaagtca atgaagcttt accacccgct gcttcaattt gttttttagc ttcctctgct
    19861 gcgtcttttc ctaaaccctc ttgaatttgt tttggtgctg attctactaa ttctttcgct
    19921 tcttttaagc ctagacctgt taaactacgt acaaccttca aaacagcaat ttttttatca
    19981 gctggaactt catcaagcat tagattaaat tctgtttttt cttcaacttc ttcaactacg
    20041 gctggagcag ctgccattac aacaccacca ctagctgctg ctgaagcatc gacaccaaat
    20101 gtttcttcaa tttttgttac taattctgaa gcttctagta atgttaatgt ttttaaatct
    20161 tcaacgattt gatcaatttt ttctgacata ataaatttaa atcatattat aaaattttag
    20221 ttttgagtaa aataatgaaa tgaatctaaa atttatgcaa atatctctaa gtctaacttt
    20281 acggatggtc ccattgtagt acaaattgat acacttttaa agtacttacc ttttacaccc
    20341 gacggacgat tttgctcaat tgattggtat agagctttta aattttcaac tagttgttcg
    20401 tttgtaaaat ttgattttcc aaaattaaca tgaacgattc cagttttgtc agccttatat
    20461 tcaaacttac cttttttaaa ttctgttaat gtcgattgca gagtactagt aacggtacct
    20521 gattttggtg agggcattaa gcctttgggt cctaacattc gacctaattt ggctaattta
    20581 ggcatcatgt tgggtgttgc aattaataaa tcaaaattaa tctgtccttg agtaatttga
    20641 gcaatcaatt catcatttcc aacgatatcc ccacctgagg atttagcctc ttcaaaattt
    20701 tcttcatttg tcagaactgc tataactact tgcttgccga tgccgtttgg tagtgtaaca
    20761 gtcgtacgca attgctgatc ggcatatttt ggatcaatat ttagattagc atgtaattct
    20821 actgattcaa caaattttgt tgttgctgtt tcttttaaaa cggcaatagc ctcgtctata
    20881 tttgaataaa caatattctt cgttctttcg aaattttctt tatgacgacg cgacactttc
    20941 tgcataactt ttctccgtta aaaatttatt taatcttcac ttaatctaaa atggagatac
    21001 ccatattacg agccgttcct tcaacaattt tcatagcact actgattttc gtagtattta
    21061 aatctagtaa tttaatgttt gcaatttctt ctaactgagt ttttgttatt gacccaacac
    21121 ttaccgtatt aggattcgac gaacccttct taatttttgc agcatttgct aatagtactg
    21181 aagcaggagg tgttttcaaa acgaatgtgt aacttctatc ttcataaact gaaatttcta
    21241 caggaataat taatcctgct ttatcattag tacgagcatt atactcttta caaaatgcgg
    21301 caatatttac accgtgttga cctaaagctg gtcccacagg cggtgccgga gtcgctttac
    21361 tagcaggtaa agctaactta atcaacgcag taattttttt ggccatacaa atataatatt
    21421 atagtttgct aataattatt ttaatcgttt taatttaata gacttataac ttgattactc
    21481 gaaatcaaaa cgttatagtt gttctaaaat aagttctatg atattaaaaa attaaaaacc
    21541 tcttaagaaa gagaaacgcg ccctgaagga cttgaaccct cgacatctaa ttttggagac
    21601 tagcgttcta ccaactgaac taaggacgcc cgagttcatt gtaaacttaa taaattgaaa
    21661 ttacaagcct aaattttaat ccatattttt atttcattaa atgcaacaaa ctccattcaa
    21721 tgttgatcaa ttaattatcg ataaatattt acctcataat ttcttagcgg aaaaaatgat
    21781 tcttagttgt ctactcatta attcagaagc aatcgaaata acattacgta aactacccta
    21841 tgaagctttt tattttaaaa atcatcaaga aatttataaa gccgttatga ttttatataa
    21901 gaataaaaag tgtatagata ttctaacctt actcactttt ttacaagata atgggctttt
    21961 acaaaaaatt ggtggagtta aagttttaat tgaattaatg agtcaaatcc ctaatttggt
    22021 ttaccttgaa gagtatatta gtttggtaaa agataagttt attagacgtt ctttaattaa
    22081 acttggttat gaagtcatta attcaggtta tattactaat cttcctcttg aacaaatatt
    22141 aggcgatttt gaaaataagc tattcaattt aacaaatgaa attacctcgc aaaaattatt
    22201 tagtagtgca gagttactta ataatatttt tttcgagtta aaagaaaaat cattaaatcc
    22261 ttcacttttc ggattacctt ccggtttcta tgaccttgat tctttaacac aaggttttca
    22321 gaaatcggat ttgattatta tcgcagctcg accatctatg ggaaaaacag cgttaggatt
    22381 aaatattgca atcaatacaa ttaaaaattc gagactaccg gttttatttc tgagcttaga
    22441 aatgtcgaaa gaacaaatta tgtatcgttt attagcaatg gaaacaaata ttaatcaaat
    22501 gcgcctccga agcggcaagc tttatcaaaa tgattggatt aaattaaata aaattataaa
    22561 aatcgtttct aaattaccgt tttttattga tgacacatct aatttatcaa ttcaagatat
    22621 tcgatcgaaa atcaaaacaa ttttatttga acaaaaccga attggtttga taataataga
    22681 ttatcttcag ttattacaaa attcagataa taagagtgaa aatcgtgtcc aagaactctc
    22741 aaatataact cgttccttaa aaacgattgc gcgggaattt aatgttccga ttattgcttt
    22801 atcccaatta agtcgaaatg ttgaaaatag actagataaa aagccaattt tatccgattt
    22861 acgagaaagt ggctctattg aacaagatgc tgatttagta ttaatgcttt atcgaaatga
    22921 atactataat gaaaacaaat ctttagataa gacaaaacaa ttgactgaac tcattattgc
    22981 aaaacaacga aatggtccgg tcggaacagt aaaattaaaa tttgatgaaa aaagaacgaa
    23041 atttcttaat gcaaacgacg cagacgtaaa tgtagttagc taatgcccag aaccagattc
    23101 gaactggtga cacgaggatc ttcaatccac tgctctacca actgagctat ccgggcttcc
    23161 aaatatattg tagcacgatc taaagaaaat tacaataaag ttttaaatta gtttgcagtt
    23221 gacggttttt caaaatttgc ctataataag ctttgggtat agtatttgat ataattttga
    23281 atttgtttag tatgtcagga tcgtaagata gcagcataaa ataaaatatc aaggttagta
    23341 ttaaatctat atctatatgc cggatccaat tttttttagt tttagttatt cgtcaatttc
    23401 ttagtcagta tctcaataat atgaaaaata tgattcgatt aatattaatt atattttttt
    23461 tacaaaagtt gatacaatat tcattgtagt caatcttact atttttaacc ttattatttt
    23521 aaacaacagg aaataaaaaa tgacaccttc attaggaagt tttatcgcta gtttatttgc
    23581 tggtgcgttt attgcattag cgattggcgg ggttttagtt tttatcagtc aaagtgatcg
    23641 cgttacgaga agttagtttt gtaatatagt agagttagat attatattga ttttagagtc
    23701 aacaaaatta attttttata aagtacatat taaaattaca gatttaattt ataaatcgca
    23761 tatttattta tactatttct agctaatata atataaagtt tatttaaagt tagctatatt
    23821 atgataaatt ttagtttcgg tccaaatatt tttttaggaa ttatcgtaag tttgggagtg
    23881 ttaatacttt attttcttag aaatgttaag cccgaagtag cgagagatga agatatcttt
    23941 tttgccacta ttggtctatt atacagttgt attctaatga tccatggttg gagattagat
    24001 ccaattcttc tttttagtca agttctaatt attgtgacag tattagtagc gggatgggaa
    24061 aatattcgac tacggggttt aattgctaac atggcaaaat taacaaagga aaaaaggaag
    24121 taaactttct cgttttatta tcttggtttc aaatttgtaa ataataattt taaattatgt
    24181 ttaagaagtc ttctcaactc ttttctttag tatttttcac tatttttagt atttttatag
    24241 gaacggcatc agctattgat ttagacgaag caacccgaac agtcgttgtc gattcttcag
    24301 gtaaaacaat cgttttaact cctgaacaag ttaaacgtgg gaaaagatta tttaatgcaa
    24361 cttgtggtgc ttgccatgtt ggtggagtta caaaaacaaa tccaaacgta ggtttagatc
    24421 cagaagcatt aagtttagcg acacctcgtc gcgataacat agcaggtttg gttgattttt
    24481 taaaaaatcc aacaacatat gatggtttag aatcaattgc tgaagttcat ccaagtatca
    24541 aaagtgcgga catttatcct cgtatgcgtt cagtaacaga tgaagattta actgccatgg
    24601 ctggtcatat attattacaa ccgaaaattg taacagaaaa atggggcggc ggtaaaatct
    24661 attattaata aaattgttta aaaagtggaa aaatttccac tttttaaaca attttattaa
    24721 taatagatta gtatgcttta aagtgaaaat gtaatagttc tggaaagcat gtagctcagt
    24781 ggatagagca tcagattccg attctggaag tcaagggttc gattcccttc atgcttatgg
    24841 ttccattttt ataatatttt aaaaatatgt ggggctgatc tggtttcgac atttaaaaat
    24901 aactaatgta tgattcaggg cgaagcttgt atccttcgta aaaatctaca aaattttaaa
    24961 taaatgctat taatataatt tctttcgttt ctaaagcagt aatttttaat attaaaaaaa
    25021 attattcaaa tttattacaa tttgctgttt aactgagtaa ctaaatttta ttacctaatt
    25081 ttgttcacta catctaatgg ctttaaacct ttgtagttta gactaatttt attaaacatt
    25141 tgttctttga aactatacct gtgaacgagt acattagata tttttaaatg gacatgggtt
    25201 caattcccat cagttccatt tttgcattcg tggccgagtg gttgaaggca ccggactcat
    25261 aatccgtttt cctctggaac gtcactggtt cgaacccagt cgaatgcaga aaatatattt
    25321 taatgttgtt tttttctaga tctaattgta agctttttct tataaaaata attacaataa
    25381 tcgttagaat tcatgttaaa gttaaagact caaaaggtaa tagatgctat agaaagtaaa
    25441 tttattaaaa aagatcttcc gacacttaaa attggtgata atgttagaat tggtgttaaa
    25501 attatcgaag gcactaaaga aagagttcag ttttacgagg gaactattat tgcaaagaaa
    25561 aatagttcta ttaatatgac aattactgtt cgaaaaatat tacagggtat cggaattgag
    25621 cgtgtttttt tagttcattc gccaaaaatt gattctatta cagttttacg ttcatcaaaa
    25681 gttcgtcgag cgaaactata ctatctacga aatttacgtg gaaaagctag tcgtttaaaa
    25741 caaactttta aatagaattt aaaatctttt aaaattttaa taaaaaaaat ttctttgtat
    25801 caaatttacg tagtataatg taatgtagta aggtggaaga aaatatttta ctttataatt
    25861 attaaacata aggagttata tggttactta caaagtgaca ttattaagtg aagaacatga
    25921 tatcaataca acaattgatt gtaatgatga tgtatttgtt ttagatgctg ctgaggagca
    25981 aggtattgaa ttaccttatt catgtcgcgc aggtgcatga agagcctggt tgccctcgcc
    26041 gctgggctgg cggccgtcta tggccctgca aacgcgccag aaacgccgtc gaagccgtgt
    26101 gcgagacacc gcggccggcc gccggcgttg tggatacctc gcggaaaact tggccctcac
    26161 tgacagatga ggggcggacg ttgacacttg aggggccgac tcacccggcg cggcgttgac
    26221 agatgagggg caggctcgat ttcggccggc gacgtggagc tggccagcct cgcaaatcgg
    26281 cgaaaacgcc tgattttacg cgagtttccc acagatgatg tggacaagcc tggggataag
    26341 tgccctgcgg tattgacact tgaggggcgc gactactgac agatgagggg cgcgatcctt
    26401 gacacttgag gggcagagtg ctgacagatg aggggcgcac ctattgacat ttgaggggct
    26461 gtccacaggc agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta
    26521 acctgtcttt taacctgctt ttaaaccaat atttataaac cttgttttta accagggctg
    26581 cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc gaaccctccc
    26641 ggtcgagtga gcgaggaagc accagggaac agcacttata tattctgctt acacacgatg
    26701 cctgaaaaaa cttcccttgg ggttatccac ttatccacgg ggatattttt ataattattt
    26761 tttttatagt ttttagatct tcttttttag agcgccttgt aggcctttat ccatgctggt
    26821 tctagagaag gtgttgtgac aaattgccct ttcagtgtga caaatcaccc tcaaatgaca
    26881 gtcctgtctg tgacaaattg cccttaaccc tgtgacaaat tgccctcaga agaagctgtt
    26941 ttttcacaaa gttatccctg cttattgact cttttttatt tagtgtgaca atctaaaaac
    27001 ttgtcacact tcacatggat ctgtc
//
